Restriction map for
pFN21AE3241
(Circular) (Six-base) MAPSORT of: pFN21AE3241.seq Check: 6457 from: 1 to: 6394
pFN21AE3241 6394 bp
With 182 enzymes: SgfI *
March 28, 2012 12:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2259 2934 2259
Size: 675 5719
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4204 278
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2946 3441 3737 1937
Size: 1009 495 296 4594
Fragments arranged by size:
4594 1009 495 296
Acc65I G'GTAC_C
Cuts at: 2832 3715 2832
Size: 883 5511
AclI AA'CG_TT
Cuts at: 1563 4521 4894 1563
Size: 2958 373 3063
Fragments arranged by size:
3063 2958 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2566 2702 3236 4457 5841 6109 1632
Size: 934 136 534 1221 1384 268 1917
Fragments arranged by size:
1917 1384 1221 934 534 268 136
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6197
AflIII A'CryG_T
Cuts at: 1203 3600 5293 6270 1203
Size: 2397 1693 977 1327
Fragments arranged by size:
2397 1693 1327 977
AgeI A'CCGG_T
Cuts at: 5225 5225
Size: 6394
AhdI GACnn_n'nnGTC
Cuts at: 5123 5123
Size: 6394
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6394
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2555 2587 2555
Size: 32 6362
AlwNI CAG_nnn'CTG
Cuts at: 2347 2449 4137 5709 2347
Size: 102 1688 1572 3032
Fragments arranged by size:
3032 1688 1572 102
ApaLI G'TGCA_C
Cuts at: 4451 5607 4451
Size: 1156 5238
ApoI r'AATT_y
Cuts at: 1493 3515 3527 3703 3929 4081 6190 1493
Size: 2022 12 176 226 152 2109 1697
Fragments arranged by size:
2109 2022 1697 226 176 152 12
AseI AT'TA_AT
Cuts at: 160 1032 4948 160
Size: 872 3916 1606
Fragments arranged by size:
3916 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2094 2963 3086 3719 1870
Size: 73 115 36 869 123 633 4545
Fragments arranged by size:
4545 869 633 123 115 73 36
BamHI G'GATC_C
Cuts at: 3724 4091 3724
Size: 367 6027
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2832 3467 3715 5170 618
Size: 325 217 627 1045 635 248 1455 1842
Fragments arranged by size:
1842 1455 1045 635 627 325 248 217
BanII G_rGCy'C
Cuts at: 1391 2268 2439 3093 3187 3240 3656 3713 1391
Size: 877 171 654 94 53 416 57 4072
Fragments arranged by size:
4072 877 654 416 171 94 57 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5423
BbvCI CC'TCA_GC
Cuts at: 2165 2165
Size: 6394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2734 2768 4585 4619 1903
Size: 34 797 34 1817 34 3678
Fragments arranged by size:
3678 1817 797 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4286 5496 4286
Size: 1210 5184
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6394
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2465 5005 136
Size: 107 122 71 631 605 793 2540 1525
Fragments arranged by size:
2540 1525 793 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6389 6389
Size: 6394
BlpI GC'TnA_GC
Cuts at: 3811 3811
Size: 6394
Bme1580I G_kGCm'C
Cuts at: 1790 3358 4455 5611 1790
Size: 1568 1097 1156 2573
Fragments arranged by size:
2573 1568 1156 1097
BmgBI CAC'GTC
Cuts at: 1318 3463 1318
Size: 2145 4249
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2335 4125 5083
Size: 416 53 83 399 24 918 1790 958
Cuts at: 5083 442
Size: 1753
Fragments arranged by size:
1790 1753 958 918 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2192 2282 2294 2939 3317 3403 3413 3640
Size: 953 90 12 645 378 86 10 227
Cuts at: 3640 5054 1239
Size: 1414 2579
Fragments arranged by size:
2579 1414 953 645 378 227 90 86
12 10
Bpu10I CC'TnA_GC
Cuts at: 2165 2165
Size: 6394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 2942 4519 5384 5682 2105
Size: 837 1577 865 298 2817
Fragments arranged by size:
2817 1577 865 837 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 3329 5057 882
Size: 617 519 1311 1728 2219
Fragments arranged by size:
2219 1728 1311 617 519
BsaAI yAC'GTr
Cuts at: 493 1204 4185 493
Size: 711 2981 2702
Fragments arranged by size:
2981 2702 711
BsaBI GATnn'nnATC
Cuts at: 2807 6388 2807
Size: 3581 2813
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4201 4583 5218 275
Size: 53 83 186 936 2668 382 635 1451
Fragments arranged by size:
2668 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2997 3222 4827 5225 5499 5646 6057 2997
Size: 225 1605 398 274 147 411 3334
Fragments arranged by size:
3334 1605 411 398 274 225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 3101 3131 3183
Size: 30 165 30 1152 30 516 30 52
Cuts at: 3183 3213 1178
Size: 30 4359
Fragments arranged by size:
4359 1152 516 165 52 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2870 3119 3578 3652 1186
Size: 1684 249 459 74 3928
Fragments arranged by size:
3928 1684 459 249 74
BseYI C'CCAG_C
Cuts at: 2550 3398 5597 6093 2550
Size: 848 2199 496 2851
Fragments arranged by size:
2851 2199 848 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2912 1163
Size: 573 1176 4645
Fragments arranged by size:
4645 1176 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2640 3844 4605 5633 1540
Size: 275 186 639 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 639 275 186
BsiHKAI G_wGCw'C
Cuts at: 2268 2925 3093 4455 4540 5611 2268
Size: 657 168 1362 85 1071 3051
Fragments arranged by size:
3051 1362 1071 657 168 85
BsmI GAATG_Cn'
Cuts at: 3905 3998 3905
Size: 93 6301
BsmBI CGTCTCn'nnnn_
Cuts at: 3569 3569
Size: 6394
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2439 2925 3093 3187 3240 3358
Size: 399 478 171 486 168 94 53 118
Cuts at: 3358 3656 3713 4455 4540 5611 1391
Size: 298 57 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 486 478 399 298 171
168 118 94 85 57 53
BspEI T'CCGG_A
Cuts at: 2997 6057 2997
Size: 3060 3334
BspHI T'CATG_A
Cuts at: 2811 4283 6013 2811
Size: 1472 1730 3192
Fragments arranged by size:
3192 1730 1472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 2736 2934 3347 3749 844
Size: 1415 477 198 413 402 3489
Fragments arranged by size:
3489 1415 477 413 402 198
BsrBI CCG'CTC
Cuts at: 1394 1823 4281 1394
Size: 429 2458 3507
Fragments arranged by size:
3507 2458 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2998 4889 5063 65
Size: 1183 1750 1891 174 1396
Fragments arranged by size:
1891 1750 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2254 2639 5038 5225 1700
Size: 110 444 385 2399 187 2869
Fragments arranged by size:
2869 2399 444 385 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6394
BssHII G'CGCG_C
Cuts at: 1925 2881 1925
Size: 956 5438
BssSI C'ACGA_G
Cuts at: 4454 5466 4454
Size: 1012 5382
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6394
BstBI TT'CG_AA
Cuts at: 5198 5198
Size: 6394
BstEII G'GTnAC_C
Cuts at: 1184 3142 1184
Size: 1958 4436
BstYI r'GATC_y
Cuts at: 1972 2424 3000 3724 4091 4476 4493 5945 6031
Size: 452 576 724 367 385 17 1452 86
Cuts at: 6031 6043 6389 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 724 576 452 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2519 2519
Size: 6394
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2556 3021 3120 3187 513
Size: 552 939 24 528 465 99 67 3720
Fragments arranged by size:
3720 939 552 528 465 99 67 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2035 487
Size: 683 56 809 4846
Fragments arranged by size:
4846 809 683 56
BtsI GCAGTG_nn'
Cuts at: 2537 3910 4704 4724 2537
Size: 1373 794 20 4207
Fragments arranged by size:
4207 1373 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6394
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6359
DraI TTT'AAA
Cuts at: 3698 3698
Size: 6394
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2585 2993 5401 809
Size: 507 1269 408 2408 1802
Fragments arranged by size:
2408 1802 1269 507 408
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2112 3018
Size: 54 1425 50 275 18 204 78 906
Cuts at: 3018 3165 3841 4730 6359 8
Size: 147 676 889 1629 43
Fragments arranged by size:
1629 1425 906 889 676 275 204 147
78 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3841 1537
Size: 275 2029 4090
Fragments arranged by size:
4090 2029 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2372 2444 2681 2894 3371 4324 1353
Size: 1019 72 237 213 477 953 3423
Fragments arranged by size:
3423 1019 953 477 237 213 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2953 4981 5355 5501 387
Size: 1064 138 1364 2028 374 146 1280
Fragments arranged by size:
2028 1364 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2266 3091 2266
Size: 825 5569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2192 2282 2294 2566 2702 2939 3236
Size: 393 560 90 12 272 136 237 297
Cuts at: 3236 3317 3403 3413 3640 4457 5054 5841 6109
Size: 81 86 10 227 817 597 787 268
Cuts at: 6109 1239
Size: 1524
Fragments arranged by size:
1524 817 787 597 560 393 297 272
268 237 227 136 90 86 81 12
10
EcoNI CCTnn'n_nnAGG
Cuts at: 2384 2453 3368 2384
Size: 69 915 5410
Fragments arranged by size:
5410 915 69
EcoO109I rG'GnC_Cy
Cuts at: 2414 2587 3066 3173 3304 3373 2414
Size: 173 479 107 131 69 5435
Fragments arranged by size:
5435 479 173 131 107 69
EcoRI G'AATT_C
Cuts at: 3515 3703 4081 3515
Size: 188 378 5828
Fragments arranged by size:
5828 378 188
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 6394
FspI TGC'GCA
Cuts at: 4900 4900
Size: 6394
HaeII r_GCGC'y
Cuts at: 1678 3293 3319 3610 5541 1678
Size: 1615 26 291 1931 2531
Fragments arranged by size:
2531 1931 1615 291 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2811 2843 5035
Size: 32 135 32 428 32 779 32 2192
Cuts at: 5035 5067 5109 5141 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2192 779 428 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3738 3984 669
Size: 988 281 1800 246 3079
Fragments arranged by size:
3079 1800 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6394
HpaI GTT'AAC
Cuts at: 3984 3984
Size: 6394
KpnI G_GTAC'C
Cuts at: 2836 3719 2836
Size: 883 5511
MfeI C'AATT_G
Cuts at: 3993 3993
Size: 6394
MluI A'CGCG_T
Cuts at: 6270 6270
Size: 6394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5508 5692 1305
Size: 4203 184 2007
Fragments arranged by size:
4203 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 2114 10
Size: 54 1425 343 204 78 4290
Fragments arranged by size:
4290 1425 343 204 78 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4352 4711 4870 518
Size: 648 3186 359 159 2042
Fragments arranged by size:
3186 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2134 3391 3810 4487 5635 5880 1606
Size: 528 1257 419 677 1148 245 2120
Fragments arranged by size:
2120 1257 1148 677 528 419 245
NaeI GCC'GGC
Cuts at: 2256 2256
Size: 6394
NcoI C'CATG_G
Cuts at: 513 1065 2004 2556 513
Size: 552 939 552 4351
Fragments arranged by size:
4351 939 552 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6394
NgoMIV G'CCGG_C
Cuts at: 2254 2254
Size: 6394
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6394
NotI GC'GGCC_GC
Cuts at: 3841 3841
Size: 6394
NspI r_CATG'y
Cuts at: 1132 1229 3752 5297 1132
Size: 97 2523 1545 2229
Fragments arranged by size:
2523 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3136 1348
Size: 1788 4606
PciI A'CATG_T
Cuts at: 5293 5293
Size: 6394
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2347 2557 3555 1108
Size: 1239 210 998 3947
Fragments arranged by size:
3947 1239 998 210
PfoI T'CCnGG_A
Cuts at: 2379 2427 2490 2574 2871 2379
Size: 48 63 84 297 5902
Fragments arranged by size:
5902 297 84 63 48
PmeI GTTT'AAAC
Cuts at: 3698 3698
Size: 6394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2555 2587 4463 4495 5976 6008 2555
Size: 32 1876 32 1481 32 2941
Fragments arranged by size:
2941 1876 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2587 3066 3173 3304 3373 2587
Size: 479 107 131 69 5608
Fragments arranged by size:
5608 479 131 107 69
PshAI GACnn'nnGTC
Cuts at: 1613 2793 1613
Size: 1180 5214
PsiI TTA'TAA
Cuts at: 3964 3964
Size: 6394
PstI C_TGCA'G
Cuts at: 830 2375 2457 2532 3099 3746 830
Size: 1545 82 75 567 647 3478
Fragments arranged by size:
3478 1545 647 567 82 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6394
PvuII CAG'CTG
Cuts at: 3391 3391
Size: 6394
SacI G_AGCT'C
Cuts at: 2268 3093 2268
Size: 825 5569
SalI G'TCGA_C
Cuts at: 1936 3736 1936
Size: 1800 4594
SanDI GG'GwC_CC
Cuts at: 3066 3066
Size: 6394
SapI GCTCTTCn'nnn_
Cuts at: 2444 2444
Size: 6394
SbfI CC_TGCA'GG
Cuts at: 3099 3746 3099
Size: 647 5747
ScaI AGT'ACT
Cuts at: 4642 6354 4642
Size: 1712 4682
SfcI C'TryA_G
Cuts at: 826 1045 2371 2453 2528 3095 3742 4877 5558
Size: 219 1326 82 75 567 647 1135 681
Cuts at: 5558 5749 826
Size: 191 1471
Fragments arranged by size:
1471 1326 1135 681 647 567 219 191
82 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6394
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6394
SmaI CCC'GGG
Cuts at: 1872 2096 3721 1872
Size: 224 1625 4545
Fragments arranged by size:
4545 1625 224
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 2957 4498 5399 5661 820
Size: 197 926 177 837 1541 901 262 1553
Fragments arranged by size:
1553 1541 926 901 837 262 197 177
SnaBI TAC'GTA
Cuts at: 493 4185 493
Size: 3692 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6394
SphI G_CATG'C
Cuts at: 1132 3752 1132
Size: 2620 3774
SspI AAT'ATT
Cuts at: 5 52 4318 5
Size: 47 4266 2081
Fragments arranged by size:
4266 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6394
StyI C'CwwG_G
Cuts at: 513 1065 2004 2556 3010 3051 3833 513
Size: 552 939 552 454 41 782 3074
Fragments arranged by size:
3074 939 782 552 552 454 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3455 4421 4438 4591 4776 5221 325
Size: 1255 1875 966 17 153 185 445 1498
Fragments arranged by size:
1875 1498 1255 966 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2511 2737 4640
Size: 274 80 33 51 1443 534 226 1903
Cuts at: 4640 6352 96
Size: 1712 138
Fragments arranged by size:
1903 1712 1443 534 274 226 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3183 3215 6354 6386 3183
Size: 32 3139 32 3191
Fragments arranged by size:
3191 3139 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6394
XbaI T'CTAG_A
Cuts at: 3730 3730
Size: 6394
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6394
XmaI C'CCGG_G
Cuts at: 1870 2094 3719 1870
Size: 224 1625 4545
Fragments arranged by size:
4545 1625 224
XmnI GAAnn'nnTTC
Cuts at: 3519 4523 3519
Size: 1004 5390
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4202 276
Size: 53 83 186 936 2668 2468
Fragments arranged by size:
2668 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI