Restriction map for
pFN21AE3237
(Circular) (Six-base) MAPSORT of: pFN21AE3237.seq Check: 376 from: 1 to: 6361
pFN21AE3237 6361 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 4171 278
Size: 53 83 186 936 2026 609 2468
Fragments arranged by size:
2468 2026 936 609 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3704 1937
Size: 1767 4594
Acc65I G'GTAC_C
Cuts at: 3682 3682
Size: 6361
AclI AA'CG_TT
Cuts at: 1563 2830 4488 4861 1563
Size: 1267 1658 373 3063
Fragments arranged by size:
3063 1658 1267 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2038 3447 4424 5808 6076 1632
Size: 406 1409 977 1384 268 1917
Fragments arranged by size:
1917 1409 1384 977 406 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6164
AflIII A'CryG_T
Cuts at: 1203 5260 6237 1203
Size: 4057 977 1327
Fragments arranged by size:
4057 1327 977
AgeI A'CCGG_T
Cuts at: 5192 5192
Size: 6361
AhdI GACnn_n'nnGTC
Cuts at: 5090 5090
Size: 6361
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6361
AlwNI CAG_nnn'CTG
Cuts at: 4104 5676 4104
Size: 1572 4789
ApaLI G'TGCA_C
Cuts at: 4418 5574 4418
Size: 1156 5205
ApoI r'AATT_y
Cuts at: 1493 2404 2486 2519 2696 3002 3188 3248 3386
Size: 911 82 33 177 306 186 60 138
Cuts at: 3386 3670 3896 4048 6157 1493
Size: 284 226 152 2109 1697
Fragments arranged by size:
2109 1697 911 306 284 226 186 177
152 138 82 60 33
AseI AT'TA_AT
Cuts at: 160 1032 4915 160
Size: 872 3883 1606
Fragments arranged by size:
3883 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
AvaI C'yCGr_G
Cuts at: 1870 1943 3686 1870
Size: 73 1743 4545
Fragments arranged by size:
4545 1743 73
BamHI G'GATC_C
Cuts at: 3691 4058 3691
Size: 367 5994
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3682 5137 618
Size: 325 217 627 1895 1455 1842
Fragments arranged by size:
1895 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3680 1391
Size: 2289 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2889 3144 928
Size: 971 990 255 4145
Fragments arranged by size:
4145 990 971 255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4552 4586 1903
Size: 34 2615 34 3678
Fragments arranged by size:
3678 2615 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4253 5463 4253
Size: 1210 5151
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6361
BfrBI ATG'CAT
Cuts at: 2808 2808
Size: 6361
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4972 136
Size: 107 122 71 631 605 3300 1525
Fragments arranged by size:
3300 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6356 6356
Size: 6361
BlpI GC'TnA_GC
Cuts at: 3778 3778
Size: 6361
Bme1580I G_kGCm'C
Cuts at: 1790 4422 5578 1790
Size: 2632 1156 2573
Fragments arranged by size:
2632 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6361
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4092 5050 442
Size: 416 53 83 399 24 2675 958 1753
Fragments arranged by size:
2675 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3135 3480 5021 1239
Size: 1896 345 1541 2579
Fragments arranged by size:
2579 1896 1541 345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4486 5351 5649 4486
Size: 865 298 5198
Fragments arranged by size:
5198 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3111 5024 882
Size: 617 1612 1913 2219
Fragments arranged by size:
2219 1913 1612 617
BsaAI yAC'GTr
Cuts at: 493 1204 4152 493
Size: 711 2948 2702
Fragments arranged by size:
2948 2702 711
BsaBI GATnn'nnATC
Cuts at: 2547 6355 2547
Size: 3808 2553
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3559 4168 4550 5185
Size: 53 83 186 936 2026 609 382 635
Cuts at: 5185 275
Size: 1451
Fragments arranged by size:
2026 1451 936 635 609 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4794 5192 5466 5613 6024 4794
Size: 398 274 147 411 5131
Fragments arranged by size:
5131 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3478 3508 1178
Size: 30 165 30 2075 30 4031
Fragments arranged by size:
4031 2075 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3272 3327 1186
Size: 2086 55 4220
Fragments arranged by size:
4220 2086 55
BseYI C'CCAG_C
Cuts at: 5564 6060 5564
Size: 496 5865
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5788
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3811 4572 5600 1540
Size: 275 186 1810 761 1028 2301
Fragments arranged by size:
2301 1810 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4422 4507 5578 4422
Size: 85 1071 5205
Fragments arranged by size:
5205 1071 85
BsmI GAATG_Cn'
Cuts at: 3353 3872 3965 3353
Size: 519 93 5749
Fragments arranged by size:
5749 519 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2474 2474
Size: 6361
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3680 4422 4507 5578 1391
Size: 399 1890 742 85 1071 2174
Fragments arranged by size:
2174 1890 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6024 6024
Size: 6361
BspHI T'CATG_A
Cuts at: 4250 5980 4250
Size: 1730 4631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3211 3716 844
Size: 2367 505 3489
Fragments arranged by size:
3489 2367 505
BsrBI CCG'CTC
Cuts at: 1394 1823 4248 1394
Size: 429 2425 3507
Fragments arranged by size:
3507 2425 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4856 5030 65
Size: 1183 3608 174 1396
Fragments arranged by size:
3608 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5005 5192 1700
Size: 110 3195 187 2869
Fragments arranged by size:
3195 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6361
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6361
BssSI C'ACGA_G
Cuts at: 4421 5433 4421
Size: 1012 5349
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6361
BstBI TT'CG_AA
Cuts at: 2401 5165 2401
Size: 2764 3597
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6361
BstYI r'GATC_y
Cuts at: 1972 2614 2996 3691 4058 4443 4460 5912 5998
Size: 642 382 695 367 385 17 1452 86
Cuts at: 5998 6010 6356 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 695 642 385 382 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2067 513
Size: 552 939 63 4807
Fragments arranged by size:
4807 939 552 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5622
Fragments arranged by size:
5622 683 56
BtsI GCAGTG_nn'
Cuts at: 2565 3475 3877 4671 4691 2565
Size: 910 402 794 20 4235
Fragments arranged by size:
4235 910 794 402 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6361
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6326
DraI TTT'AAA
Cuts at: 3665 3665
Size: 6361
DraIII CAC_nnn'GTG
Cuts at: 2446 2446
Size: 6361
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2057 5368 809
Size: 507 741 3311 1802
Fragments arranged by size:
3311 1802 741 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 3808 4697
Size: 54 1425 50 275 18 192 1786 889
Cuts at: 4697 6326 8
Size: 1629 43
Fragments arranged by size:
1786 1629 1425 889 275 192 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3808 1537
Size: 275 1996 4090
Fragments arranged by size:
4090 1996 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3147 4291 1353
Size: 1794 1144 3423
Fragments arranged by size:
3423 1794 1144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4948 5322 5468 387
Size: 1064 138 3359 374 146 1280
Fragments arranged by size:
3359 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 3135 3447 3480 4424 5021 5808
Size: 393 406 1097 312 33 944 597 787
Cuts at: 5808 6076 1239
Size: 268 1524
Fragments arranged by size:
1524 1097 944 787 597 406 393 312
268 33
EcoRI G'AATT_C
Cuts at: 3002 3188 3386 3670 4048 3002
Size: 186 198 284 378 5315
Fragments arranged by size:
5315 378 284 198 186
EcoRV GAT'ATC
Cuts at: 2872 2872
Size: 6361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2906 2906
Size: 6361
FspI TGC'GCA
Cuts at: 4867 4867
Size: 6361
HaeII r_GCGC'y
Cuts at: 1678 5508 1678
Size: 3830 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2606 2638 3131 3163 5002
Size: 32 135 32 1034 32 493 32 1839
Cuts at: 5002 5034 5076 5108 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1839 1034 493 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2229 3531 3705 3951 669
Size: 988 281 291 1302 174 246 3079
Fragments arranged by size:
3079 1302 988 291 281 246 174
HindIII A'AGCT_T
Cuts at: 748 2372 3198 748
Size: 1624 826 3911
Fragments arranged by size:
3911 1624 826
HpaI GTT'AAC
Cuts at: 3951 3951
Size: 6361
KpnI G_GTAC'C
Cuts at: 3686 3686
Size: 6361
MfeI C'AATT_G
Cuts at: 2538 3960 2538
Size: 1422 4939
MluI A'CGCG_T
Cuts at: 6237 6237
Size: 6361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2275 2882 5475 5659 1305
Size: 970 607 2593 184 2007
Fragments arranged by size:
2593 2007 970 607 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2024 10
Size: 54 1425 343 192 4347
Fragments arranged by size:
4347 1425 343 192 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2772 4319 4678 4837 518
Size: 648 1606 1547 359 159 2042
Fragments arranged by size:
2042 1606 1547 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2069 3551 3777 4454 5602 5847 1606
Size: 463 1482 226 677 1148 245 2120
Fragments arranged by size:
2120 1482 1148 677 463 245 226
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4870
Fragments arranged by size:
4870 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6361
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6361
NotI GC'GGCC_GC
Cuts at: 3808 3808
Size: 6361
NsiI A_TGCA'T
Cuts at: 2810 2810
Size: 6361
NspI r_CATG'y
Cuts at: 1132 1229 3616 3719 5264 1132
Size: 97 2387 103 1545 2229
Fragments arranged by size:
2387 2229 1545 103 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6361
PciI A'CATG_T
Cuts at: 5260 5260
Size: 6361
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3651 1108
Size: 2543 3818
PmeI GTTT'AAAC
Cuts at: 3665 3665
Size: 6361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3430 3462 4430 4462 5943 5975 3430
Size: 32 968 32 1481 32 3816
Fragments arranged by size:
3816 1481 968 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6361
PsiI TTA'TAA
Cuts at: 3931 3931
Size: 6361
PstI C_TGCA'G
Cuts at: 830 3713 830
Size: 2883 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6361
PvuII CAG'CTG
Cuts at: 3551 3551
Size: 6361
SacII CC_GC'GG
Cuts at: 2070 2070
Size: 6361
SalI G'TCGA_C
Cuts at: 1936 3703 1936
Size: 1767 4594
SbfI CC_TGCA'GG
Cuts at: 3713 3713
Size: 6361
ScaI AGT'ACT
Cuts at: 4609 6321 4609
Size: 1712 4649
SfcI C'TryA_G
Cuts at: 826 1045 2124 3709 4844 5525 5716 826
Size: 219 1079 1585 1135 681 191 1471
Fragments arranged by size:
1585 1471 1135 1079 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6361
SmaI CCC'GGG
Cuts at: 1872 3688 1872
Size: 1816 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4465 5366 5628 820
Size: 197 926 2522 901 262 1553
Fragments arranged by size:
2522 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4152 493
Size: 3659 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6361
SphI G_CATG'C
Cuts at: 1132 3719 1132
Size: 2587 3774
SspI AAT'ATT
Cuts at: 5 52 2980 4285 5
Size: 47 2928 1305 2081
Fragments arranged by size:
2928 2081 1305 47
StuI AGG'CCT
Cuts at: 1517 3394 1517
Size: 1877 4484
StyI C'CwwG_G
Cuts at: 513 1065 2004 3800 513
Size: 552 939 1796 3074
Fragments arranged by size:
3074 1796 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 4388 4405 4558 4743 5188 325
Size: 1255 717 2091 17 153 185 445 1498
Fragments arranged by size:
2091 1498 1255 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4607 6319 96
Size: 274 80 33 51 1443 2630 1712 138
Fragments arranged by size:
2630 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6321 6353 6321
Size: 32 6329
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6361
XbaI T'CTAG_A
Cuts at: 3079 3697 3079
Size: 618 5743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2309 2309
Size: 6361
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6361
XmaI C'CCGG_G
Cuts at: 1870 3686 1870
Size: 1816 4545
XmnI GAAnn'nnTTC
Cuts at: 2100 2831 2949 4490 2100
Size: 731 118 1541 3971
Fragments arranged by size:
3971 1541 731 118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 4169 276
Size: 53 83 186 936 2026 609 2468
Fragments arranged by size:
2468 2026 936 609 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI