Restriction map for
pFN21AE3235
(Circular) (Six-base) MAPSORT of: pFN21AE3235.seq Check: 3302 from: 1 to: 6349
pFN21AE3235 6349 bp
With 182 enzymes: SgfI *
March 28, 2012 12:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3692 1937
Size: 1755 4594
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2540 3005 4412 5796 6064 1632
Size: 908 465 1407 1384 268 1917
Fragments arranged by size:
1917 1407 1384 908 465 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 5248 6225 1203
Size: 4045 977 1327
Fragments arranged by size:
4045 1327 977
AgeI A'CCGG_T
Cuts at: 3188 5180 3188
Size: 1992 4357
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 2950 3191 3524 1166
Size: 1784 241 333 3991
Fragments arranged by size:
3991 1784 333 241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2968 3171 3269 1184
Size: 1784 203 98 4264
Fragments arranged by size:
4264 1784 203 98
AlwNI CAG_nnn'CTG
Cuts at: 4092 5664 4092
Size: 1572 4777
ApaLI G'TGCA_C
Cuts at: 2891 3476 4406 5562 2891
Size: 585 930 1156 3678
Fragments arranged by size:
3678 1156 930 585
ApoI r'AATT_y
Cuts at: 1493 3658 3884 4036 6145 1493
Size: 2165 226 152 2109 1697
Fragments arranged by size:
2165 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 3302 3674 1870
Size: 73 121 1238 372 4545
Fragments arranged by size:
4545 1238 372 121 73
BamHI G'GATC_C
Cuts at: 3679 4046 3679
Size: 367 5982
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2542 3263 3670 5125 618
Size: 325 217 627 755 721 407 1455 1842
Fragments arranged by size:
1842 1455 755 721 627 407 325 217
BanII G_rGCy'C
Cuts at: 1391 2661 3228 3303 3309 3668 1391
Size: 1270 567 75 6 359 4072
Fragments arranged by size:
4072 1270 567 359 75 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2342 928
Size: 971 443 4935
Fragments arranged by size:
4935 971 443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4540 4574 1903
Size: 34 2603 34 3678
Fragments arranged by size:
3678 2603 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 2355 1548
Size: 807 5542
BfrBI ATG'CAT
Cuts at: 3494 3494
Size: 6349
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3395 4960 136
Size: 107 122 71 631 605 1723 1565 1525
Fragments arranged by size:
1723 1565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2673 3438 6344 2673
Size: 765 2906 2678
Fragments arranged by size:
2906 2678 765
BlpI GC'TnA_GC
Cuts at: 2975 3766 2975
Size: 791 5558
Bme1580I G_kGCm'C
Cuts at: 1790 2162 2379 2547 2895 3480 4410 5566 1790
Size: 372 217 168 348 585 930 1156 2573
Fragments arranged by size:
2573 1156 930 585 372 348 217 168
BmgBI CAC'GTC
Cuts at: 1318 2049 3253 3424 1318
Size: 731 1204 171 4243
Fragments arranged by size:
4243 1204 731 171
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2172 4080 5038
Size: 416 53 83 399 24 755 1908 958
Cuts at: 5038 442
Size: 1753
Fragments arranged by size:
1908 1753 958 755 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2924 5009 1239
Size: 1685 2085 2579
Fragments arranged by size:
2579 2085 1685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2495 4474 5339 5637 2495
Size: 1979 865 298 3207
Fragments arranged by size:
3207 1979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2093 3029 5012 882
Size: 617 594 936 1983 2219
Fragments arranged by size:
2219 1983 936 617 594
BsaAI yAC'GTr
Cuts at: 493 1204 2890 3349 4140 493
Size: 711 1686 459 791 2702
Fragments arranged by size:
2702 1686 791 711 459
BsaBI GATnn'nnATC
Cuts at: 6343 6343
Size: 6349
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4156 4538 5173 275
Size: 53 83 186 936 2623 382 635 1451
Fragments arranged by size:
2623 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3188 4782 5180 5454 5601 6012 3188
Size: 1594 398 274 147 411 3525
Fragments arranged by size:
3525 1594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2675 2705 3086 3116 1178
Size: 30 165 30 1272 30 381 30 4411
Fragments arranged by size:
4411 1272 381 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2921 3641 1186
Size: 1735 720 3894
Fragments arranged by size:
3894 1735 720
BseYI C'CCAG_C
Cuts at: 5552 6048 5552
Size: 496 5853
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2903 3349 1163
Size: 573 1167 446 4163
Fragments arranged by size:
4163 1167 573 446
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2258 2743 3586 3799 4560 5588
Size: 275 186 257 485 843 213 761 1028
Cuts at: 5588 1540
Size: 2301
Fragments arranged by size:
2301 1028 843 761 485 275 257 213
186
BsiHKAI G_wGCw'C
Cuts at: 2661 2895 2993 3096 3309 3411 3480 4410 4495
Size: 234 98 103 213 102 69 930 85
Cuts at: 4495 5566 2661
Size: 1071 3444
Fragments arranged by size:
3444 1071 930 234 213 103 102 98
85 69
BsmI GAATG_Cn'
Cuts at: 3860 3953 3860
Size: 93 6256
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2379 2547 2661 2895 2993 3096
Size: 399 372 217 168 114 234 98 103
Cuts at: 3096 3228 3303 3309 3411 3480 3668 4410 4495
Size: 132 75 6 102 69 188 742 85
Cuts at: 4495 5566 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 372 234 217 188
168 132 114 103 102 98 85 75
69 6
BspEI T'CCGG_A
Cuts at: 6012 6012
Size: 6349
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2989 3704 844
Size: 2145 715 3489
Fragments arranged by size:
3489 2145 715
BsrBI CCG'CTC
Cuts at: 1394 1823 2853 4236 1394
Size: 429 1030 1383 3507
Fragments arranged by size:
3507 1383 1030 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2128 4844 5018 65
Size: 1183 880 2716 174 1396
Fragments arranged by size:
2716 1396 1183 880 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2079 3188 3209 4993 5180 1700
Size: 110 269 1109 21 1784 187 2869
Fragments arranged by size:
2869 1784 1109 269 187 110 21
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 3205 3235 1925
Size: 1280 30 5039
Fragments arranged by size:
5039 1280 30
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2890 794
Size: 2096 4253
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6349
BstXI CCAn_nnnn'nTGG
Cuts at: 2311 2824 3526 2311
Size: 513 702 5134
Fragments arranged by size:
5134 702 513
BstYI r'GATC_y
Cuts at: 1972 2673 3438 3679 4046 4431 4448 5900 5986
Size: 701 765 241 367 385 17 1452 86
Cuts at: 5986 5998 6344 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 765 701 385 367 346 241
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3317 3429 3317
Size: 112 6237
BtgI C'CryG_G
Cuts at: 513 1065 2004 2397 3219 3375 3519 513
Size: 552 939 393 822 156 144 3343
Fragments arranged by size:
3343 939 822 552 393 156 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3354 487
Size: 683 56 2128 3482
Fragments arranged by size:
3482 2128 683 56
BtsI GCAGTG_nn'
Cuts at: 2329 3405 3625 3865 4659 4679 2329
Size: 1076 220 240 794 20 3999
Fragments arranged by size:
3999 1076 794 240 220 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6314
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DraIII CAC_nnn'GTG
Cuts at: 3424 3484 3424
Size: 60 6289
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2837 3536 5356 809
Size: 507 1521 699 1820 1802
Fragments arranged by size:
1820 1802 1521 699 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2255 2346 2740
Size: 54 1425 50 275 18 425 91 394
Cuts at: 2740 2865 3387 3796 4685 6314 8
Size: 125 522 409 889 1629 43
Fragments arranged by size:
1629 1425 889 522 425 409 394 275
125 91 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2255 2740 3796 1537
Size: 275 443 485 1056 4090
Fragments arranged by size:
4090 1056 485 443 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2525 2981 4279 1353
Size: 1172 456 1298 3423
Fragments arranged by size:
3423 1298 1172 456
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2690 4936 5310 5456 387
Size: 1064 138 1101 2246 374 146 1280
Fragments arranged by size:
2246 1280 1101 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2659 3307 2659
Size: 648 5701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2540 2924 3005 4412 5009 5796 6064
Size: 393 908 384 81 1407 597 787 268
Cuts at: 6064 1239
Size: 1524
Fragments arranged by size:
1524 1407 908 787 597 393 384 268
81
EcoO109I rG'GnC_Cy
Cuts at: 2365 2365
Size: 6349
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 6349
FspI TGC'GCA
Cuts at: 3257 4855 3257
Size: 1598 4751
FspAI rTGC'GCAy
Cuts at: 3257 3257
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 2170 5496 1678
Size: 492 3326 2531
Fragments arranged by size:
3326 2531 492
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2675 2707 3495 3527 4990
Size: 32 135 32 1103 32 788 32 1463
Cuts at: 4990 5022 5064 5096 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1463 1103 788 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3693 3939 669
Size: 988 281 1755 246 3079
Fragments arranged by size:
3079 1755 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6349
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2634 2718 5463 5647 1305
Size: 1329 84 2745 184 2007
Fragments arranged by size:
2745 2007 1329 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2348 2867 10
Size: 54 1425 343 516 519 3492
Fragments arranged by size:
3492 1425 519 516 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2475 2950 3191 3524 4307 4666 4825
Size: 648 1309 475 241 333 783 359 159
Cuts at: 4825 518
Size: 2042
Fragments arranged by size:
2042 1309 783 648 475 359 333 241
159
MspA1I CmG'CkG
Cuts at: 1606 2902 2962 3765 4442 5590 5835 1606
Size: 1296 60 803 677 1148 245 2120
Fragments arranged by size:
2120 1296 1148 803 677 245 60
NaeI GCC'GGC
Cuts at: 2081 2081
Size: 6349
NcoI C'CATG_G
Cuts at: 513 1065 2004 2397 3219 513
Size: 552 939 393 822 3643
Fragments arranged by size:
3643 939 822 552 393
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NgoMIV G'CCGG_C
Cuts at: 2079 2079
Size: 6349
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 2255 3796 2255
Size: 1541 4808
NruI TCG'CGA
Cuts at: 3045 3045
Size: 6349
NsiI A_TGCA'T
Cuts at: 3496 3496
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 3013 3707 5252 1132
Size: 97 1784 694 1545 2229
Fragments arranged by size:
2229 1784 1545 694 97
PasI CC'CwG_GG
Cuts at: 1348 2379 1348
Size: 1031 5318
PciI A'CATG_T
Cuts at: 5248 5248
Size: 6349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3569 1108
Size: 2461 3888
PfoI T'CCnGG_A
Cuts at: 2661 3411 2661
Size: 750 5599
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PmlI CAC'GTG
Cuts at: 2890 3349 2890
Size: 459 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PstI C_TGCA'G
Cuts at: 830 3701 830
Size: 2871 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 2902 2902
Size: 6349
SacI G_AGCT'C
Cuts at: 2661 3309 2661
Size: 648 5701
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SapI GCTCTTCn'nnn_
Cuts at: 2981 2981
Size: 6349
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SfcI C'TryA_G
Cuts at: 826 1045 3697 4832 5513 5704 826
Size: 219 2652 1135 681 191 1471
Fragments arranged by size:
2652 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 3188 3209 1700
Size: 1488 21 4840
Fragments arranged by size:
4840 1488 21
SmaI CCC'GGG
Cuts at: 1872 3676 1872
Size: 1804 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2510 4453 5354 5616 820
Size: 197 926 567 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 567 262 197
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3013 3707 1132
Size: 1881 694 3774
Fragments arranged by size:
3774 1881 694
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 2297 1517
Size: 780 5569
StyI C'CwwG_G
Cuts at: 513 1065 2004 2397 3219 3327 3788 513
Size: 552 939 393 822 108 461 3074
Fragments arranged by size:
3074 939 822 552 461 393 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2371 2487 4376 4393 4546 4731 5176
Size: 1255 791 116 1889 17 153 185 445
Cuts at: 5176 325
Size: 1498
Fragments arranged by size:
1889 1498 1255 791 445 185 153 116
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2769 2802 4595
Size: 274 80 33 51 1443 792 33 1793
Cuts at: 4595 6307 96
Size: 1712 138
Fragments arranged by size:
1793 1712 1443 792 274 138 80 51
33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3086 3118 6309 6341 3086
Size: 32 3191 32 3094
Fragments arranged by size:
3191 3094 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3125 3605 1316
Size: 1809 480 4060
Fragments arranged by size:
4060 1809 480
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2821 2821
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 3674 1870
Size: 1804 4545
XmnI GAAnn'nnTTC
Cuts at: 4478 4478
Size: 6349
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV
FseI KasI NarI PacI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI