Restriction map for
pFN21AE3233
(Circular) (Six-base) MAPSORT of: pFN21AE3233.seq Check: 7889 from: 1 to: 6334
pFN21AE3233 6334 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2842 2991 2842
Size: 149 6185
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2223 3677 1937
Size: 286 1454 4594
Fragments arranged by size:
4594 1454 286
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2402 2616 3621 4397 5781 6049 1632
Size: 557 213 214 1005 776 1384 268 1917
Fragments arranged by size:
1917 1384 1005 776 557 268 214 213
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 5233 6210 1203
Size: 4030 977 1327
Fragments arranged by size:
4030 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 5063 5063
Size: 6334
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6334
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 6302
AlwNI CAG_nnn'CTG
Cuts at: 2536 4077 5649 2536
Size: 1541 1572 3221
Fragments arranged by size:
3221 1572 1541
ApaI G_GGCC'C
Cuts at: 2560 3252 2560
Size: 692 5642
ApaLI G'TGCA_C
Cuts at: 4391 5547 4391
Size: 1156 5178
ApoI r'AATT_y
Cuts at: 1493 2780 3643 3869 4021 6130 1493
Size: 1287 863 226 152 2109 1697
Fragments arranged by size:
2109 1697 1287 863 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2745 3055 3659 1870
Size: 73 80 722 310 604 4545
Fragments arranged by size:
4545 722 604 310 80 73
BamHI G'GATC_C
Cuts at: 3664 4031 3664
Size: 367 5967
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2238 3655 5110 618
Size: 325 217 627 451 1417 1455 1842
Fragments arranged by size:
1842 1455 1417 627 451 325 217
BanII G_rGCy'C
Cuts at: 1391 2024 2063 2560 2886 2927 3252 3653 1391
Size: 633 39 497 326 41 325 401 4072
Fragments arranged by size:
4072 633 497 401 326 325 41 39
BbeI G_GCGC'C
Cuts at: 2242 2242
Size: 6334
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3349 3501 3575 928
Size: 971 1450 152 74 3687
Fragments arranged by size:
3687 1450 971 152 74
BbvCI CC'TCA_GC
Cuts at: 2564 2564
Size: 6334
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2594 2628 4525 4559 1903
Size: 34 657 34 1897 34 3678
Fragments arranged by size:
3678 1897 657 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4226 5436 4226
Size: 1210 5124
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6334
BfrBI ATG'CAT
Cuts at: 2012 2012
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4945 136
Size: 107 122 71 631 605 3273 1525
Fragments arranged by size:
3273 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2088 6329 2088
Size: 4241 2093
BlpI GC'TnA_GC
Cuts at: 3153 3322 3751 3153
Size: 169 429 5736
Fragments arranged by size:
5736 429 169
Bme1580I G_kGCm'C
Cuts at: 1790 2262 2560 3122 3252 4395 5551 1790
Size: 472 298 562 130 1143 1156 2573
Fragments arranged by size:
2573 1156 1143 562 472 298 130
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4065 5023 442
Size: 416 53 83 399 24 2648 958 1753
Fragments arranged by size:
2648 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2077 2852 2858 3023 4994 1239
Size: 838 775 6 165 1971 2579
Fragments arranged by size:
2579 1971 838 775 165 6
Bpu10I CC'TnA_GC
Cuts at: 2564 2564
Size: 6334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2996 3505 4459 5324 5622 2015
Size: 981 509 954 865 298 2727
Fragments arranged by size:
2727 981 954 865 509 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2033 2924 4997 882
Size: 617 534 891 2073 2219
Fragments arranged by size:
2219 2073 891 617 534
BsaAI yAC'GTr
Cuts at: 493 1204 4125 493
Size: 711 2921 2702
Fragments arranged by size:
2921 2702 711
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2239 4141 4523 5158
Size: 53 83 186 936 706 1902 382 635
Cuts at: 5158 275
Size: 1451
Fragments arranged by size:
1902 1451 936 706 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2799 4767 5165 5439 5586 5997 2799
Size: 1968 398 274 147 411 3136
Fragments arranged by size:
3136 1968 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6109
Fragments arranged by size:
6109 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2894 3104 3160 3494 1186
Size: 1708 210 56 334 4026
Fragments arranged by size:
4026 1708 334 210 56
BseYI C'CCAG_C
Cuts at: 2261 2292 2696 2863 3403 3576 5537 6033 2261
Size: 31 404 167 540 173 1961 496 2562
Fragments arranged by size:
2562 1961 540 496 404 173 167 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3012 1163
Size: 573 1276 4485
Fragments arranged by size:
4485 1276 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3784 4545 5573 1540
Size: 275 186 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2379 2920 4395 4480 5551 2379
Size: 541 1475 85 1071 3162
Fragments arranged by size:
3162 1475 1071 541 85
BsmI GAATG_Cn'
Cuts at: 2012 3845 3938 2012
Size: 1833 93 4408
Fragments arranged by size:
4408 1833 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2063 2262 2379 2560 2886 2920
Size: 399 234 39 199 117 181 326 34
Cuts at: 2920 2927 3122 3252 3653 4395 4480 5551 1391
Size: 7 195 130 401 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 401 399 326 234 199
195 181 130 117 85 39 34 7
BspEI T'CCGG_A
Cuts at: 2799 5997 2799
Size: 3198 3136
BspHI T'CATG_A
Cuts at: 4223 5953 4223
Size: 1730 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2842 2991 3689 844
Size: 1998 149 698 3489
Fragments arranged by size:
3489 1998 698 149
BsrBI CCG'CTC
Cuts at: 1394 1823 3007 4221 1394
Size: 429 1184 1214 3507
Fragments arranged by size:
3507 1214 1184 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2952 4829 5003 65
Size: 1183 1704 1877 174 1396
Fragments arranged by size:
1877 1704 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4978 5165 1700
Size: 110 3168 187 2869
Fragments arranged by size:
3168 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6334
BssSI C'ACGA_G
Cuts at: 3380 4394 5406 3380
Size: 1014 1012 4308
Fragments arranged by size:
4308 1014 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2536 794
Size: 1742 4592
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstYI r'GATC_y
Cuts at: 1972 2088 3346 3664 4031 4416 4433 5885 5971
Size: 116 1258 318 367 385 17 1452 86
Cuts at: 5971 5983 6329 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1258 385 367 346 318 116
86 17 12
BstZ17I GTA'TAC
Cuts at: 2224 2224
Size: 6334
Bsu36I CC'TnA_GG
Cuts at: 3477 3477
Size: 6334
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4843
Fragments arranged by size:
4843 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5595
Fragments arranged by size:
5595 683 56
BtsI GCAGTG_nn'
Cuts at: 2104 2139 2979 3636 3850 4644 4664 2104
Size: 35 840 657 214 794 20 3774
Fragments arranged by size:
3774 840 794 657 214 35 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DraIII CAC_nnn'GTG
Cuts at: 2327 2327
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2437 5341 809
Size: 507 1121 2904 1802
Fragments arranged by size:
2904 1802 1121 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3781 4670 6299
Size: 54 1425 50 275 18 1951 889 1629
Cuts at: 6299 8
Size: 43
Fragments arranged by size:
1951 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2382 2592 3105 3543 4264 1353
Size: 1029 210 513 438 721 3423
Fragments arranged by size:
3423 1029 721 513 438 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2612 4921 5295 5441 387
Size: 1064 138 1023 2309 374 146 1280
Fragments arranged by size:
2309 1280 1064 1023 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2077 2189 2402 2616 2852 2858 3023
Size: 393 445 112 213 214 236 6 165
Cuts at: 3023 3621 4397 4994 5781 6049 1239
Size: 598 776 597 787 268 1524
Fragments arranged by size:
1524 787 776 598 597 445 393 268
236 214 213 165 112 6
EcoO109I rG'GnC_Cy
Cuts at: 2557 3249 2557
Size: 692 5642
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FspI TGC'GCA
Cuts at: 4840 4840
Size: 6334
HaeII r_GCGC'y
Cuts at: 1678 2242 2299 5481 1678
Size: 564 57 3182 2531
Fragments arranged by size:
3182 2531 564 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2075 2077 2107 2459
Size: 32 135 32 473 30 2 30 352
Cuts at: 2459 2491 2708 2740 4975 5007 5049 5081 1373
Size: 32 217 32 2235 32 42 32 2626
Fragments arranged by size:
2626 2235 473 352 217 135 42 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3678 3924 669
Size: 988 281 1740 246 3079
Fragments arranged by size:
3079 1740 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6334
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KasI G'GCGC_C
Cuts at: 2238 2238
Size: 6334
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2097 5448 5632 1305
Size: 792 3351 184 2007
Fragments arranged by size:
3351 2007 792 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4512
Fragments arranged by size:
4512 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2822 4292 4651 4810 518
Size: 648 1656 1470 359 159 2042
Fragments arranged by size:
2042 1656 1470 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2115 2185 3033 3157 3407 3750 4427 5575
Size: 509 70 848 124 250 343 677 1148
Cuts at: 5575 5820 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 848 677 509 343 250 245
124 70
NarI GG'CG_CC
Cuts at: 2239 2239
Size: 6334
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4843
Fragments arranged by size:
4843 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NsiI A_TGCA'T
Cuts at: 2014 2014
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 2725 3692 5237 1132
Size: 97 1496 967 1545 2229
Fragments arranged by size:
2229 1545 1496 967 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6334
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6334
PfoI T'CCnGG_A
Cuts at: 2943 2943
Size: 6334
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5916 5948 4403
Size: 32 1481 32 4789
Fragments arranged by size:
4789 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PspOMI G'GGCC_C
Cuts at: 2556 3248 2556
Size: 692 5642
PspXI vC'TCGA_Gb
Cuts at: 3055 3055
Size: 6334
PstI C_TGCA'G
Cuts at: 830 2532 2690 2988 3686 830
Size: 1702 158 298 698 3478
Fragments arranged by size:
3478 1702 698 298 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 2115 2185 3157 3407 2115
Size: 70 972 250 5042
Fragments arranged by size:
5042 972 250 70
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SapI GCTCTTCn'nnn_
Cuts at: 3105 3105
Size: 6334
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 4582 6294 4582
Size: 1712 4622
SexAI A'CCwGG_T
Cuts at: 2211 2211
Size: 6334
SfcI C'TryA_G
Cuts at: 826 1045 2528 2686 2984 3682 4817 5498 5689
Size: 219 1483 158 298 698 1135 681 191
Cuts at: 5689 826
Size: 1471
Fragments arranged by size:
1483 1471 1135 698 681 298 219 191
158
SfoI GGC'GCC
Cuts at: 2240 2240
Size: 6334
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 2747 3661 1872
Size: 875 914 4545
Fragments arranged by size:
4545 914 875
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 3011 3055 3520 4438 5339
Size: 197 926 87 981 44 465 918 901
Cuts at: 5339 5601 820
Size: 262 1553
Fragments arranged by size:
1553 981 926 918 901 465 262 197
87 44
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 3692 1132
Size: 2560 3774
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 3126 1517
Size: 1609 4725
StyI C'CwwG_G
Cuts at: 513 1065 2004 2242 3773 513
Size: 552 939 238 1531 3074
Fragments arranged by size:
3074 1531 939 552 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2170 3062 3219 3454 4361 4378 4531
Size: 1255 590 892 157 235 907 17 153
Cuts at: 4531 4716 5161 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 907 892 590 445 235 185
157 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4580 6292 96
Size: 274 80 33 51 1443 2603 1712 138
Fragments arranged by size:
2603 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6294 6326 6294
Size: 32 6302
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2827 2827
Size: 6334
XhoI C'TCGA_G
Cuts at: 1943 3055 1943
Size: 1112 5222
XmaI C'CCGG_G
Cuts at: 1870 2745 3659 1870
Size: 875 914 4545
Fragments arranged by size:
4545 914 875
XmnI GAAnn'nnTTC
Cuts at: 3356 4463 3356
Size: 1107 5227
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BsiWI BsmBI BstXI EcoICRI
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PmlI PpuMI PsrI RsrII SacI SacII SanDI
SfiI SrfI SwaI