Restriction map for
pFN21AE3232
(Circular) (Six-base) MAPSORT of: pFN21AE3232.seq Check: 1550 from: 1 to: 6325
pFN21AE3232 6325 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3202 3202
Size: 6325
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2613 3668 1937
Size: 676 1055 4594
Fragments arranged by size:
4594 1055 676
Acc65I G'GTAC_C
Cuts at: 3646 3646
Size: 6325
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2329 4388 5772 6040 1632
Size: 697 2059 1384 268 1917
Fragments arranged by size:
2059 1917 1384 697 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6128
AflIII A'CryG_T
Cuts at: 1203 2694 3069 5224 6201 1203
Size: 1491 375 2155 977 1327
Fragments arranged by size:
2155 1491 1327 977 375
AgeI A'CCGG_T
Cuts at: 2075 5156 2075
Size: 3081 3244
AhdI GACnn_n'nnGTC
Cuts at: 5054 5054
Size: 6325
AleI CACnn'nnGTG
Cuts at: 1166 2216 2531 1166
Size: 1050 315 4960
Fragments arranged by size:
4960 1050 315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6325
AlwNI CAG_nnn'CTG
Cuts at: 3623 4068 5640 3623
Size: 445 1572 4308
Fragments arranged by size:
4308 1572 445
ApaI G_GGCC'C
Cuts at: 3365 3365
Size: 6325
ApaLI G'TGCA_C
Cuts at: 4382 5538 4382
Size: 1156 5169
ApoI r'AATT_y
Cuts at: 1493 3634 3860 4012 6121 1493
Size: 2141 226 152 2109 1697
Fragments arranged by size:
2141 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4879 160
Size: 872 3847 1606
Fragments arranged by size:
3847 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 2214 2654 3650 1870
Size: 73 271 440 996 4545
Fragments arranged by size:
4545 996 440 271 73
BamHI G'GATC_C
Cuts at: 3655 4022 3655
Size: 367 5958
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2378 2536 3284 3646
Size: 325 217 627 285 306 158 748 362
Cuts at: 3646 5101 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 748 627 362 325 306 285
217 158
BanII G_rGCy'C
Cuts at: 1391 2877 3048 3365 3644 1391
Size: 1486 171 317 279 4072
Fragments arranged by size:
4072 1486 317 279 171
BbeI G_GCGC'C
Cuts at: 2382 2540 2382
Size: 158 6167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2665 2745 928
Size: 971 766 80 4508
Fragments arranged by size:
4508 971 766 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4516 4550 1903
Size: 34 50 34 2495 34 3678
Fragments arranged by size:
3678 2495 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4217 5427 4217
Size: 1210 5115
BclI T'GATC_A
Cuts at: 1548 2778 1548
Size: 1230 5095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4936 136
Size: 107 122 71 631 605 3264 1525
Fragments arranged by size:
3264 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6320 6320
Size: 6325
BlpI GC'TnA_GC
Cuts at: 2940 3742 2940
Size: 802 5523
Bme1580I G_kGCm'C
Cuts at: 1790 2294 3287 3365 4386 5542 1790
Size: 504 993 78 1021 1156 2573
Fragments arranged by size:
2573 1156 1021 993 504 78
BmgBI CAC'GTC
Cuts at: 1318 2697 1318
Size: 1379 4946
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2277 2461 4056
Size: 416 53 83 399 24 860 184 1595
Cuts at: 4056 5014 442
Size: 958 1753
Fragments arranged by size:
1753 1595 958 860 416 399 184 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2614 2651 2945 4985 1239
Size: 1375 37 294 2040 2579
Fragments arranged by size:
2579 2040 1375 294 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4450 5315 5613 4450
Size: 865 298 5162
Fragments arranged by size:
5162 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2711 3228 4988 882
Size: 617 1212 517 1760 2219
Fragments arranged by size:
2219 1760 1212 617 517
BsaAI yAC'GTr
Cuts at: 493 1204 4116 493
Size: 711 2912 2702
Fragments arranged by size:
2912 2702 711
BsaBI GATnn'nnATC
Cuts at: 6319 6319
Size: 6325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2128 2379 2537 4132
Size: 53 83 186 936 595 251 158 1595
Cuts at: 4132 4514 5149 275
Size: 382 635 1451
Fragments arranged by size:
1595 1451 936 635 595 382 251 186
158 83 53
BsaWI w'CCGG_w
Cuts at: 2075 2805 4758 5156 5430 5577 5988 2075
Size: 730 1953 398 274 147 411 2412
Fragments arranged by size:
2412 1953 730 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6100
Fragments arranged by size:
6100 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2696 2931 3092 1186
Size: 1510 235 161 4419
Fragments arranged by size:
4419 1510 235 161
BseYI C'CCAG_C
Cuts at: 2052 2474 3549 5528 6024 2052
Size: 422 1075 1979 496 2353
Fragments arranged by size:
2353 1979 1075 496 422
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2589 1163
Size: 573 853 4899
Fragments arranged by size:
4899 853 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2225 3341 3775 4536 5564 1540
Size: 275 186 224 1116 434 761 1028 2301
Fragments arranged by size:
2301 1116 1028 761 434 275 224 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2877 4386 4471 5542 2025
Size: 852 1509 85 1071 2808
Fragments arranged by size:
2808 1509 1071 852 85
BsmI GAATG_Cn'
Cuts at: 3836 3929 3836
Size: 93 6232
BsmBI CGTCTCn'nnnn_
Cuts at: 2016 2016
Size: 6325
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2294 2877 3048 3287 3365 3644
Size: 399 235 269 583 171 239 78 279
Cuts at: 3644 4386 4471 5542 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 583 399 279 269 239
235 171 85 78
BspEI T'CCGG_A
Cuts at: 2805 5988 2805
Size: 3183 3142
BspHI T'CATG_A
Cuts at: 4214 5944 4214
Size: 1730 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2466 3202 3680 844
Size: 1622 736 478 3489
Fragments arranged by size:
3489 1622 736 478
BsrBI CCG'CTC
Cuts at: 1394 1823 2635 4212 1394
Size: 429 812 1577 3507
Fragments arranged by size:
3507 1577 812 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4820 4994 65
Size: 1183 3572 174 1396
Fragments arranged by size:
3572 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2066 2075 2224 4969 5156 1700
Size: 110 256 9 149 2745 187 2869
Fragments arranged by size:
2869 2745 256 187 149 110 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6325
BssSI C'ACGA_G
Cuts at: 2666 4385 5397 2666
Size: 1719 1012 3594
Fragments arranged by size:
3594 1719 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6325
BstBI TT'CG_AA
Cuts at: 5129 5129
Size: 6325
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6325
BstXI CCAn_nnnn'nTGG
Cuts at: 2728 2728
Size: 6325
BstYI r'GATC_y
Cuts at: 1972 2731 3605 3655 4022 4407 4424 5876 5962
Size: 759 874 50 367 385 17 1452 86
Cuts at: 5962 5974 6320 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 874 759 385 367 346 86
50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3012 513
Size: 552 1947 3826
Fragments arranged by size:
3826 1947 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2430 487
Size: 683 56 1204 4382
Fragments arranged by size:
4382 1204 683 56
BtsI GCAGTG_nn'
Cuts at: 2800 3456 3841 4635 4655 2800
Size: 656 385 794 20 4470
Fragments arranged by size:
4470 794 656 385 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3412 3447 529
Size: 35 2848 35 3407
Fragments arranged by size:
3407 2848 35 35
DraI TTT'AAA
Cuts at: 3369 3629 3369
Size: 260 6065
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5332 809
Size: 507 4016 1802
Fragments arranged by size:
4016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2222 2226
Size: 54 1425 50 275 18 234 158 4
Cuts at: 2226 2484 3338 3772 4661 6290 8
Size: 258 854 434 889 1629 43
Fragments arranged by size:
1629 1425 889 854 434 275 258 234
158 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2222 3338 3772 1537
Size: 275 410 1116 434 4090
Fragments arranged by size:
4090 1116 434 410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2348 2882 2893 2906 4255 1353
Size: 995 534 11 13 1349 3423
Fragments arranged by size:
3423 1349 995 534 13 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2139 2570 4912 5286 5432 387
Size: 1064 138 550 431 2342 374 146 1280
Fragments arranged by size:
2342 1280 1064 550 431 374 146 138
EcoICRI GAG'CTC
Cuts at: 2875 2875
Size: 6325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2329 2614 2651 2945 4388 4985 5772
Size: 393 697 285 37 294 1443 597 787
Cuts at: 5772 6040 1239
Size: 268 1524
Fragments arranged by size:
1524 1443 787 697 597 393 294 285
268 37
EcoO109I rG'GnC_Cy
Cuts at: 2455 3361 3362 2455
Size: 906 1 5418
Fragments arranged by size:
5418 906 1
EcoRI G'AATT_C
Cuts at: 3634 4012 3634
Size: 378 5947
FseI GG_CCGG'CC
Cuts at: 2228 2228
Size: 6325
FspI TGC'GCA
Cuts at: 2246 2750 4831 2246
Size: 504 2081 3740
Fragments arranged by size:
3740 2081 504
HaeII r_GCGC'y
Cuts at: 1678 2147 2382 2429 2483 2540 5472 1678
Size: 469 235 47 54 57 2932 2531
Fragments arranged by size:
2932 2531 469 235 57 54 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2763 2795 2956 2988 4966
Size: 32 135 32 1191 32 161 32 1978
Cuts at: 4966 4998 5040 5072 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1978 1191 161 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3067 3669 3915 669
Size: 988 281 1129 602 246 3079
Fragments arranged by size:
3079 1129 988 602 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6325
HpaI GTT'AAC
Cuts at: 3067 3915 3067
Size: 848 5477
KasI G'GCGC_C
Cuts at: 2378 2536 2378
Size: 158 6167
KpnI G_GTAC'C
Cuts at: 3650 3650
Size: 6325
MfeI C'AATT_G
Cuts at: 3924 3924
Size: 6325
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5439 5623 1305
Size: 4134 184 2007
Fragments arranged by size:
4134 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4503
Fragments arranged by size:
4503 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2216 2531 2819 2832 3017 4283 4642
Size: 648 1050 315 288 13 185 1266 359
Cuts at: 4642 4801 518
Size: 159 2042
Fragments arranged by size:
2042 1266 1050 648 359 315 288 185
159 13
MspA1I CmG'CkG
Cuts at: 1606 2489 3741 4418 5566 5811 1606
Size: 883 1252 677 1148 245 2120
Fragments arranged by size:
2120 1252 1148 883 677 245
NaeI GCC'GGC
Cuts at: 2068 2226 2068
Size: 158 6167
NarI GG'CG_CC
Cuts at: 2379 2537 2379
Size: 158 6167
NcoI C'CATG_G
Cuts at: 513 1065 3012 513
Size: 552 1947 3826
Fragments arranged by size:
3826 1947 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6325
NgoMIV G'CCGG_C
Cuts at: 2066 2224 2066
Size: 158 6167
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6325
NotI GC'GGCC_GC
Cuts at: 3772 3772
Size: 6325
NspI r_CATG'y
Cuts at: 1132 1229 3073 3683 5228 1132
Size: 97 1844 610 1545 2229
Fragments arranged by size:
2229 1844 1545 610 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6325
PciI A'CATG_T
Cuts at: 3069 5224 3069
Size: 2155 4170
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6325
PfoI T'CCnGG_A
Cuts at: 2676 2676
Size: 6325
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4394 4426 5907 5939 4394
Size: 32 1481 32 4780
Fragments arranged by size:
4780 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2455 2455
Size: 6325
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6325
PsiI TTA'TAA
Cuts at: 3895 3895
Size: 6325
PspOMI G'GGCC_C
Cuts at: 3361 3361
Size: 6325
PspXI vC'TCGA_Gb
Cuts at: 2654 2654
Size: 6325
PstI C_TGCA'G
Cuts at: 830 3677 830
Size: 2847 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
SacI G_AGCT'C
Cuts at: 2877 2877
Size: 6325
SalI G'TCGA_C
Cuts at: 1936 3667 1936
Size: 1731 4594
SapI GCTCTTCn'nnn_
Cuts at: 2348 2882 2893 2348
Size: 534 11 5780
Fragments arranged by size:
5780 534 11
SbfI CC_TGCA'GG
Cuts at: 3677 3677
Size: 6325
ScaI AGT'ACT
Cuts at: 2516 3129 4573 6285 2516
Size: 613 1444 1712 2556
Fragments arranged by size:
2556 1712 1444 613
SfcI C'TryA_G
Cuts at: 826 1045 2614 2704 3673 4808 5489 5680 826
Size: 219 1569 90 969 1135 681 191 1471
Fragments arranged by size:
1569 1471 1135 969 681 219 191 90
SfoI GGC'GCC
Cuts at: 2380 2538 2380
Size: 158 6167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 2075 1700
Size: 375 5950
SmaI CCC'GGG
Cuts at: 1872 2216 3652 1872
Size: 344 1436 4545
Fragments arranged by size:
4545 1436 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 4429 5330 5592 820
Size: 197 926 711 1775 901 262 1553
Fragments arranged by size:
1775 1553 926 901 711 262 197
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6325
SphI G_CATG'C
Cuts at: 1132 3683 1132
Size: 2551 3774
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6325
StyI C'CwwG_G
Cuts at: 513 1065 3012 3356 3568 3764 513
Size: 552 1947 344 212 196 3074
Fragments arranged by size:
3074 1947 552 344 212 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3252 3302 4352 4369 4522 4707 5152
Size: 1255 1672 50 1050 17 153 185 445
Cuts at: 5152 325
Size: 1498
Fragments arranged by size:
1672 1498 1255 1050 445 185 153 50
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 3127 4571
Size: 274 80 33 51 1443 537 613 1444
Cuts at: 4571 6283 96
Size: 1712 138
Fragments arranged by size:
1712 1444 1443 613 537 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3450 3482 6285 6317 3450
Size: 32 2803 32 3458
Fragments arranged by size:
3458 2803 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6325
XbaI T'CTAG_A
Cuts at: 3661 3661
Size: 6325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2770 3271 3296 2770
Size: 501 25 5799
Fragments arranged by size:
5799 501 25
XhoI C'TCGA_G
Cuts at: 1943 2654 1943
Size: 711 5614
XmaI C'CCGG_G
Cuts at: 1870 2214 3650 1870
Size: 344 1436 4545
Fragments arranged by size:
4545 1436 344
XmnI GAAnn'nnTTC
Cuts at: 4454 4454
Size: 6325
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SrfI SwaI