Restriction map for
pFN21AE3231
(Circular) (Six-base) MAPSORT of: pFN21AE3231.seq Check: 4412 from: 1 to: 6322
pFN21AE3231 6322 bp
With 182 enzymes: SgfI *
March 28, 2012 12:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2127 2127
Size: 6322
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4132 278
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3665 1937
Size: 1728 4594
Acc65I G'GTAC_C
Cuts at: 2112 3643 2112
Size: 1531 4791
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2936 3194 3569 4385 5769 6037 1632
Size: 1304 258 375 816 1384 268 1917
Fragments arranged by size:
1917 1384 1304 816 375 268 258
AflII C'TTAA_G
Cuts at: 820 1017 3397 820
Size: 197 2380 3745
Fragments arranged by size:
3745 2380 197
AflIII A'CryG_T
Cuts at: 1203 5221 6198 1203
Size: 4018 977 1327
Fragments arranged by size:
4018 1327 977
AgeI A'CCGG_T
Cuts at: 5153 5153
Size: 6322
AhdI GACnn_n'nnGTC
Cuts at: 5051 5051
Size: 6322
AleI CACnn'nnGTG
Cuts at: 1166 2789 1166
Size: 1623 4699
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6322
AlwNI CAG_nnn'CTG
Cuts at: 2599 2892 2911 3001 3217 3385 3492 4065 5637
Size: 293 19 90 216 168 107 573 1572
Cuts at: 5637 2599
Size: 3284
Fragments arranged by size:
3284 1572 573 293 216 168 107 90
19
ApaI G_GGCC'C
Cuts at: 2228 2325 2228
Size: 97 6225
ApaLI G'TGCA_C
Cuts at: 4379 5535 4379
Size: 1156 5166
ApoI r'AATT_y
Cuts at: 1493 3631 3857 4009 6118 1493
Size: 2138 226 152 2109 1697
Fragments arranged by size:
2138 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4876 160
Size: 872 3844 1606
Fragments arranged by size:
3844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 2107 2220 2312 3453 3496 3540
Size: 73 100 64 113 92 1141 43 44
Cuts at: 3540 3647 1870
Size: 107 4545
Fragments arranged by size:
4545 1141 113 107 100 92 73 64
44 43
BamHI G'GATC_C
Cuts at: 3652 4019 3652
Size: 367 5955
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2095 2112 2296 3318 3449
Size: 325 217 627 308 17 184 1022 131
Cuts at: 3449 3643 5098 618
Size: 194 1455 1842
Fragments arranged by size:
1842 1455 1022 627 325 308 217 194
184 131 17
BanII G_rGCy'C
Cuts at: 1391 2044 2108 2228 2259 2325 3087 3101 3416
Size: 653 64 120 31 66 762 14 315
Cuts at: 3416 3641 1391
Size: 225 4072
Fragments arranged by size:
4072 762 653 315 225 120 66 64
31 14
BbeI G_GCGC'C
Cuts at: 2099 2099
Size: 6322
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5351
BbvCI CC'TCA_GC
Cuts at: 2988 2988
Size: 6322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4513 4547 1903
Size: 34 2576 34 3678
Fragments arranged by size:
3678 2576 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4214 5424 4214
Size: 1210 5112
BclI T'GATC_A
Cuts at: 1548 3483 1548
Size: 1935 4387
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3258 4933 136
Size: 107 122 71 631 605 1586 1675 1525
Fragments arranged by size:
1675 1586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6317 6317
Size: 6322
BlpI GC'TnA_GC
Cuts at: 3739 3739
Size: 6322
Bme1580I G_kGCm'C
Cuts at: 1790 2228 2325 3452 4383 5539 1790
Size: 438 97 1127 931 1156 2573
Fragments arranged by size:
2573 1156 1127 931 438 97
BmgBI CAC'GTC
Cuts at: 1318 3264 1318
Size: 1946 4376
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4053 5011 442
Size: 416 53 83 399 24 2636 958 1753
Fragments arranged by size:
2636 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2729 1056
Size: 1673 4649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2576 2579 2888 2933 2978 3254 3395 3539
Size: 1337 3 309 45 45 276 141 144
Cuts at: 3539 3590 3611 4982 1239
Size: 51 21 1371 2579
Fragments arranged by size:
2579 1371 1337 309 276 144 141 51
45 45 21 3
Bpu10I CC'TnA_GC
Cuts at: 2258 2988 2258
Size: 730 5592
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2663 3364 4447 5312 5610 2663
Size: 701 1083 865 298 3375
Fragments arranged by size:
3375 1083 865 701 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2334 4985 882
Size: 617 835 2651 2219
Fragments arranged by size:
2651 2219 835 617
BsaAI yAC'GTr
Cuts at: 493 1204 4113 493
Size: 711 2909 2702
Fragments arranged by size:
2909 2702 711
BsaBI GATnn'nnATC
Cuts at: 6316 6316
Size: 6322
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2096 4129 4511 5146
Size: 53 83 186 936 563 2033 382 635
Cuts at: 5146 275
Size: 1451
Fragments arranged by size:
2033 1451 936 635 563 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4755 5153 5427 5574 5985 4755
Size: 398 274 147 411 5092
Fragments arranged by size:
5092 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2801 2831 1178
Size: 30 165 30 1398 30 4669
Fragments arranged by size:
4669 1398 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2822 3095 1186
Size: 1636 273 4413
Fragments arranged by size:
4413 1636 273
BseYI C'CCAG_C
Cuts at: 2014 2228 2334 2881 2898 3421 3617 5525 6021
Size: 214 106 547 17 523 196 1908 496
Cuts at: 6021 2014
Size: 2315
Fragments arranged by size:
2315 1908 547 523 496 214 196 106
17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2387 1163
Size: 573 651 5098
Fragments arranged by size:
5098 651 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2070 2277 2509 3772 4533 5561
Size: 275 186 69 207 232 1263 761 1028
Cuts at: 5561 1540
Size: 2301
Fragments arranged by size:
2301 1263 1028 761 275 232 207 186
69
BsiHKAI G_wGCw'C
Cuts at: 2924 3087 3101 3434 4383 4468 5539 2924
Size: 163 14 333 949 85 1071 3707
Fragments arranged by size:
3707 1071 949 333 163 85 14
BsmI GAATG_Cn'
Cuts at: 2346 2829 2943 3833 3926 2346
Size: 483 114 890 93 4742
Fragments arranged by size:
4742 890 483 114 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2108 2228 2259 2325 2924 3087
Size: 399 254 64 120 31 66 599 163
Cuts at: 3087 3101 3416 3434 3452 3641 4383 4468 5539
Size: 14 315 18 18 189 742 85 1071
Cuts at: 5539 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 599 399 315 254 189
163 120 85 66 64 31 18 18
14
BspEI T'CCGG_A
Cuts at: 5985 5985
Size: 6322
BspHI T'CATG_A
Cuts at: 4211 5941 4211
Size: 1730 4592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2017 2127 3113 3168 3677 844
Size: 1173 110 986 55 509 3489
Fragments arranged by size:
3489 1173 986 509 110 55
BsrBI CCG'CTC
Cuts at: 1394 1823 2064 4209 1394
Size: 429 241 2145 3507
Fragments arranged by size:
3507 2145 429 241
BsrDI GCAATG_nn'
Cuts at: 65 1248 4817 4991 65
Size: 1183 3569 174 1396
Fragments arranged by size:
3569 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2088 2293 2508 2924 3199 3270 4966
Size: 110 278 205 215 416 275 71 1696
Cuts at: 4966 5153 1700
Size: 187 2869
Fragments arranged by size:
2869 1696 416 278 275 215 205 187
110 71
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6322
BssHII G'CGCG_C
Cuts at: 1925 2188 2426 3188 1925
Size: 263 238 762 5059
Fragments arranged by size:
5059 762 263 238
BssSI C'ACGA_G
Cuts at: 4382 5394 4382
Size: 1012 5310
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2892 794
Size: 2098 4224
BstBI TT'CG_AA
Cuts at: 5126 5126
Size: 6322
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6322
BstXI CCAn_nnnn'nTGG
Cuts at: 2791 2791
Size: 6322
BstYI r'GATC_y
Cuts at: 1972 3172 3214 3652 4019 4404 4421 5873 5959
Size: 1200 42 438 367 385 17 1452 86
Cuts at: 5959 5971 6317 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1200 438 385 367 346 86
42 17 12
Bsu36I CC'TnA_GG
Cuts at: 3443 3443
Size: 6322
BtgI C'CryG_G
Cuts at: 513 1065 2004 2054 2235 2695 2772 3145 513
Size: 552 939 50 181 460 77 373 3690
Fragments arranged by size:
3690 939 552 460 373 181 77 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5583
Fragments arranged by size:
5583 683 56
BtsI GCAGTG_nn'
Cuts at: 2876 3326 3838 4632 4652 2876
Size: 450 512 794 20 4546
Fragments arranged by size:
4546 794 512 450 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6322
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6287
DraI TTT'AAA
Cuts at: 3626 3626
Size: 6322
DraIII CAC_nnn'GTG
Cuts at: 2482 2635 2701 2482
Size: 153 66 6103
Fragments arranged by size:
6103 153 66
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2491 2717 5329 809
Size: 507 1175 226 2612 1802
Fragments arranged by size:
2612 1802 1175 507 226
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2238 2274
Size: 54 1425 50 275 18 237 171 36
Cuts at: 2274 2506 3349 3769 4658 6287 8
Size: 232 843 420 889 1629 43
Fragments arranged by size:
1629 1425 889 843 420 275 237 232
171 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2067 2274 2506 3769 1537
Size: 275 255 207 232 1263 4090
Fragments arranged by size:
4090 1263 275 255 232 207
EarI CTCTTCn'nnn_
Cuts at: 1353 4252 1353
Size: 2899 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2408 2549 4909 5283 5429 387
Size: 1064 138 819 141 2360 374 146 1280
Fragments arranged by size:
2360 1280 1064 819 374 146 141 138
EcoICRI GAG'CTC
Cuts at: 3085 3099 3085
Size: 14 6308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2576 2579 2888 2933 2936 2978 3194
Size: 393 944 3 309 45 3 42 216
Cuts at: 3194 3254 3395 3539 3569 3590 3611 4385 4982
Size: 60 141 144 30 21 21 774 597
Cuts at: 4982 5769 6037 1239
Size: 787 268 1524
Fragments arranged by size:
1524 944 787 774 597 393 309 268
216 144 141 60 45 42 30 21
21 3 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3377 3377
Size: 6322
EcoO109I rG'GnC_Cy
Cuts at: 2224 2225 2321 2817 2908 3127 3370 3382 3565
Size: 1 96 496 91 219 243 12 183
Cuts at: 3565 2224
Size: 4981
Fragments arranged by size:
4981 496 243 219 183 96 91 12
1
EcoRI G'AATT_C
Cuts at: 3631 4009 3631
Size: 378 5944
FseI GG_CCGG'CC
Cuts at: 2512 2512
Size: 6322
FspI TGC'GCA
Cuts at: 2828 4828 2828
Size: 2000 4322
HaeII r_GCGC'y
Cuts at: 1678 2054 2099 2235 5469 1678
Size: 376 45 136 3234 2531
Fragments arranged by size:
3234 2531 376 136 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2801 2833 4963 4995 5037
Size: 32 135 32 1229 32 2130 32 42
Cuts at: 5037 5069 1373
Size: 32 2626
Fragments arranged by size:
2626 2130 1229 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3666 3912 669
Size: 988 281 1728 246 3079
Fragments arranged by size:
3079 1728 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6322
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KasI G'GCGC_C
Cuts at: 2095 2095
Size: 6322
KpnI G_GTAC'C
Cuts at: 2116 3647 2116
Size: 1531 4791
MfeI C'AATT_G
Cuts at: 3921 3921
Size: 6322
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2056 2154 5436 5620 1305
Size: 751 98 3282 184 2007
Fragments arranged by size:
3282 2007 751 184 98
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3351 10
Size: 54 1425 343 1519 2981
Fragments arranged by size:
2981 1519 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 4280 4639 4798 518
Size: 648 1623 1491 359 159 2042
Fragments arranged by size:
2042 1623 1491 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2185 2237 2442 2500 2789 2889 2956
Size: 450 129 52 205 58 289 100 67
Cuts at: 2956 3492 3531 3598 3738 4415 5563 5808 1606
Size: 536 39 67 140 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 536 450 289 245 205
140 129 100 67 67 58 52 39
NaeI GCC'GGC
Cuts at: 2510 3201 2510
Size: 691 5631
NarI GG'CG_CC
Cuts at: 2096 2096
Size: 6322
NcoI C'CATG_G
Cuts at: 513 1065 2004 2772 513
Size: 552 939 768 4063
Fragments arranged by size:
4063 939 768 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6322
NgoMIV G'CCGG_C
Cuts at: 2508 3199 2508
Size: 691 5631
NheI G'CTAG_C
Cuts at: 1052 2725 1052
Size: 1673 4649
NotI GC'GGCC_GC
Cuts at: 2274 3769 2274
Size: 1495 4827
NspI r_CATG'y
Cuts at: 1132 1229 2140 3680 5225 1132
Size: 97 911 1540 1545 2229
Fragments arranged by size:
2229 1545 1540 911 97
PasI CC'CwG_GG
Cuts at: 1348 3407 1348
Size: 2059 4263
PciI A'CATG_T
Cuts at: 5221 5221
Size: 6322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2755 2911 1108
Size: 1647 156 4519
Fragments arranged by size:
4519 1647 156
PfoI T'CCnGG_A
Cuts at: 2910 3037 3055 2910
Size: 127 18 6177
Fragments arranged by size:
6177 127 18
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 5904 5936 4391
Size: 32 1481 32 4777
Fragments arranged by size:
4777 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2817 2908 3127 3370 2817
Size: 91 219 243 5769
Fragments arranged by size:
5769 243 219 91
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 3892 3892
Size: 6322
PspOMI G'GGCC_C
Cuts at: 2224 2321 2224
Size: 97 6225
PstI C_TGCA'G
Cuts at: 830 2127 2976 3674 830
Size: 1297 849 698 3478
Fragments arranged by size:
3478 1297 849 698
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
PvuII CAG'CTG
Cuts at: 2789 2889 2956 3492 3598 2789
Size: 100 67 536 106 5513
Fragments arranged by size:
5513 536 106 100 67
SacI G_AGCT'C
Cuts at: 3087 3101 3087
Size: 14 6308
SacII CC_GC'GG
Cuts at: 2057 2238 2057
Size: 181 6141
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SanDI GG'GwC_CC
Cuts at: 2817 2817
Size: 6322
SbfI CC_TGCA'GG
Cuts at: 3674 3674
Size: 6322
ScaI AGT'ACT
Cuts at: 4570 6282 4570
Size: 1712 4610
SfcI C'TryA_G
Cuts at: 826 1045 2123 2762 2845 2972 3670 4805 5486
Size: 219 1078 639 83 127 698 1135 681
Cuts at: 5486 5677 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1078 698 681 639 219 191
127 83
SfoI GGC'GCC
Cuts at: 2097 2097
Size: 6322
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6322
SmaI CCC'GGG
Cuts at: 1872 2045 2222 3455 3498 3542 3649 1872
Size: 173 177 1233 43 44 107 4545
Fragments arranged by size:
4545 1233 177 173 107 44 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2678 3343 3397 4426 5327 5589
Size: 197 926 735 665 54 1029 901 262
Cuts at: 5589 820
Size: 1553
Fragments arranged by size:
1553 1029 926 901 735 665 262 197
54
SnaBI TAC'GTA
Cuts at: 493 4113 493
Size: 3620 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 2140 3680 1132
Size: 1008 1540 3774
Fragments arranged by size:
3774 1540 1008
SrfI GCCC'GGGC
Cuts at: 2045 3498 2045
Size: 1453 4869
SspI AAT'ATT
Cuts at: 5 52 4246 5
Size: 47 4194 2081
Fragments arranged by size:
4194 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6322
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 2352 2517 2772 3285 3761
Size: 552 939 243 105 165 255 513 476
Cuts at: 3761 513
Size: 3074
Fragments arranged by size:
3074 939 552 513 476 255 243 165
105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2314 3435 3631 4349 4366 4519 4704
Size: 1255 734 1121 196 718 17 153 185
Cuts at: 4704 5149 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1121 734 718 445 196 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4568 6280 96
Size: 274 80 33 51 1443 2591 1712 138
Fragments arranged by size:
2591 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2066 2098 3033 3065 3566 3598 6282 6314 2066
Size: 32 935 32 501 32 2684 32 2074
Fragments arranged by size:
2684 2074 935 501 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2488 1316
Size: 1172 5150
XbaI T'CTAG_A
Cuts at: 3658 3658
Size: 6322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2410 2410
Size: 6322
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6322
XmaI C'CCGG_G
Cuts at: 1870 2043 2220 3453 3496 3540 3647 1870
Size: 173 177 1233 43 44 107 4545
Fragments arranged by size:
4545 1233 177 173 107 44 43
XmnI GAAnn'nnTTC
Cuts at: 4451 4451
Size: 6322
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4130 276
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoRV FalI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SapI SexAI SfiI SwaI