Restriction map for
pFN21AE3230
(Circular) (Six-base) MAPSORT of: pFN21AE3230.seq Check: 3712 from: 1 to: 6313
pFN21AE3230 6313 bp
With 182 enzymes: SgfI *
March 28, 2012 13:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3440 3440
Size: 6313
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3172 4123 278
Size: 53 83 186 936 1636 951 2468
Fragments arranged by size:
2468 1636 951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3540 3656 1937
Size: 1603 116 4594
Fragments arranged by size:
4594 1603 116
Acc65I G'GTAC_C
Cuts at: 3634 3634
Size: 6313
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2032 2363 4376 5760 6028 1632
Size: 400 331 2013 1384 268 1917
Fragments arranged by size:
2013 1917 1384 400 331 268
AfeI AGC'GCT
Cuts at: 2798 2798
Size: 6313
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 5212 6189 1203
Size: 4009 977 1327
Fragments arranged by size:
4009 1327 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 5042 5042
Size: 6313
AleI CACnn'nnGTG
Cuts at: 1166 3004 1166
Size: 1838 4475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3022 1184
Size: 1838 4475
AlwNI CAG_nnn'CTG
Cuts at: 2218 2338 2403 4056 5628 2218
Size: 120 65 1653 1572 2903
Fragments arranged by size:
2903 1653 1572 120 65
ApaLI G'TGCA_C
Cuts at: 2165 2715 4370 5526 2165
Size: 550 1655 1156 2952
Fragments arranged by size:
2952 1655 1156 550
ApoI r'AATT_y
Cuts at: 1493 2138 2538 2580 2662 3622 3848 4000 6109
Size: 645 400 42 82 960 226 152 2109
Cuts at: 6109 1493
Size: 1697
Fragments arranged by size:
2109 1697 960 645 400 226 152 82
42
AseI AT'TA_AT
Cuts at: 160 1032 4867 160
Size: 872 3835 1606
Fragments arranged by size:
3835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 3523 3572 3597 3638 1870
Size: 73 612 968 49 25 41 4545
Fragments arranged by size:
4545 968 612 73 49 41 25
BamHI G'GATC_C
Cuts at: 3643 4010 3643
Size: 367 5946
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2708 2903 3634 5089 618
Size: 325 217 627 921 195 731 1455 1842
Fragments arranged by size:
1842 1455 921 731 627 325 217 195
BanII G_rGCy'C
Cuts at: 1391 2329 2940 3632 1391
Size: 938 611 692 4072
Fragments arranged by size:
4072 938 692 611
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2089 928
Size: 971 190 5152
Fragments arranged by size:
5152 971 190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2003 2037 4504 4538 1903
Size: 34 66 34 2467 34 3678
Fragments arranged by size:
3678 2467 66 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3067 4205 5415 3067
Size: 1138 1210 3965
Fragments arranged by size:
3965 1210 1138
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6313
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2333 3407 4924
Size: 107 122 71 631 605 661 1074 1517
Cuts at: 4924 136
Size: 1525
Fragments arranged by size:
1525 1517 1074 661 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2128 6308 2128
Size: 4180 2133
BlpI GC'TnA_GC
Cuts at: 2738 3730 2738
Size: 992 5321
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2719 2906 3468 4374 5530 1790
Size: 379 550 187 562 906 1156 2573
Fragments arranged by size:
2573 1156 906 562 550 379 187
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2431 2847 3553
Size: 416 53 83 399 24 1014 416 706
Cuts at: 3553 4044 5002 442
Size: 491 958 1753
Fragments arranged by size:
1753 1014 958 706 491 416 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2178 2343 2407 2471 2796 2894 3088 4973
Size: 939 165 64 64 325 98 194 1885
Cuts at: 4973 1239
Size: 2579
Fragments arranged by size:
2579 1885 939 325 194 165 98 64
64
Bpu10I CC'TnA_GC
Cuts at: 2472 2472
Size: 6313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2250 4438 5303 5601 2250
Size: 2188 865 298 2962
Fragments arranged by size:
2962 2188 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4976 882
Size: 617 3477 2219
Fragments arranged by size:
3477 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4104 493
Size: 711 2900 2702
Fragments arranged by size:
2900 2702 711
BsaBI GATnn'nnATC
Cuts at: 6307 6307
Size: 6313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3169 4120 4502 5137
Size: 53 83 186 936 1636 951 382 635
Cuts at: 5137 275
Size: 1451
Fragments arranged by size:
1636 1451 951 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3607 4746 5144 5418 5565 5976 3607
Size: 1139 398 274 147 411 3944
Fragments arranged by size:
3944 1139 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2343 2373 2375 2405 1178
Size: 30 165 30 940 30 2 30 5086
Fragments arranged by size:
5086 940 165 30 30 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2744 3339 1186
Size: 1558 595 4160
Fragments arranged by size:
4160 1558 595
BseYI C'CCAG_C
Cuts at: 2040 2328 2848 2985 5516 6012 2040
Size: 288 520 137 2531 496 2341
Fragments arranged by size:
2531 2341 520 496 288 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5740
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4524 5552 1540
Size: 275 186 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 2169 2473 2719 3235 4374 4459 5530 2016
Size: 153 304 246 516 1139 85 1071 2799
Fragments arranged by size:
2799 1139 1071 516 304 246 153 85
BsmI GAATG_Cn'
Cuts at: 3206 3824 3917 3206
Size: 618 93 5602
Fragments arranged by size:
5602 618 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2169 2329 2473 2719 2906 2940
Size: 399 226 153 160 144 246 187 34
Cuts at: 2940 3235 3468 3632 4374 4459 5530 1391
Size: 295 233 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 295 246 233 226
187 164 160 153 144 85 34
BspEI T'CCGG_A
Cuts at: 3607 5976 3607
Size: 2369 3944
BspHI T'CATG_A
Cuts at: 3027 4202 5932 3027
Size: 1175 1730 3408
Fragments arranged by size:
3408 1730 1175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2050 3440 3668 844
Size: 1206 1390 228 3489
Fragments arranged by size:
3489 1390 1206 228
BsrBI CCG'CTC
Cuts at: 1394 1823 4200 1394
Size: 429 2377 3507
Fragments arranged by size:
3507 2377 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2886 4808 4982 65
Size: 1183 1638 1922 174 1396
Fragments arranged by size:
1922 1638 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4957 5144 1700
Size: 110 3147 187 2869
Fragments arranged by size:
3147 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 4373 5385 4373
Size: 1012 5301
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6313
BstBI TT'CG_AA
Cuts at: 5117 5117
Size: 6313
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstXI CCAn_nnnn'nTGG
Cuts at: 3103 3103
Size: 6313
BstYI r'GATC_y
Cuts at: 1972 2128 3039 3643 4010 4395 4412 5864 5950
Size: 156 911 604 367 385 17 1452 86
Cuts at: 5950 5962 6308 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 911 604 385 367 346 156
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4822
Fragments arranged by size:
4822 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5574
Fragments arranged by size:
5574 683 56
BtsI GCAGTG_nn'
Cuts at: 3086 3396 3829 4623 4643 3086
Size: 310 433 794 20 4756
Fragments arranged by size:
4756 794 433 310 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6278
DraI TTT'AAA
Cuts at: 3013 3617 3013
Size: 604 5709
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2891 5320 809
Size: 507 1575 2429 1802
Fragments arranged by size:
2429 1802 1575 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3255 3315 3760
Size: 54 1425 50 275 18 1425 60 445
Cuts at: 3760 4649 6278 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1425 889 445 275 60 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 1537
Size: 275 1948 4090
Fragments arranged by size:
4090 1948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4243 1353
Size: 2890 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4900 5274 5420 387
Size: 1064 138 435 2876 374 146 1280
Fragments arranged by size:
2876 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2178 2343 2363 2407 2471 2796
Size: 393 400 146 165 20 44 64 325
Cuts at: 2796 2894 3088 4376 4973 5760 6028 1239
Size: 98 194 1288 597 787 268 1524
Fragments arranged by size:
1524 1288 787 597 400 393 325 268
194 165 146 98 64 44 20
EcoO109I rG'GnC_Cy
Cuts at: 2496 3547 3602 2496
Size: 1051 55 5207
Fragments arranged by size:
5207 1051 55
EcoRI G'AATT_C
Cuts at: 3622 4000 3622
Size: 378 5935
EcoRV GAT'ATC
Cuts at: 2224 2224
Size: 6313
FspI TGC'GCA
Cuts at: 4819 4819
Size: 6313
HaeII r_GCGC'y
Cuts at: 1678 2800 5460 1678
Size: 1122 2660 2531
Fragments arranged by size:
2660 2531 1122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 3024 3041 3056
Size: 32 135 32 803 32 617 17 15
Cuts at: 3056 3073 4954 4986 5028 5060 1373
Size: 17 1881 32 42 32 2626
Fragments arranged by size:
2626 1881 803 617 135 42 32 32
32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2980 3657 3903 669
Size: 988 281 1042 677 246 3079
Fragments arranged by size:
3079 1042 988 677 281 246
HindIII A'AGCT_T
Cuts at: 748 2344 2735 748
Size: 1596 391 4326
Fragments arranged by size:
4326 1596 391
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KpnI G_GTAC'C
Cuts at: 3638 3638
Size: 6313
MfeI C'AATT_G
Cuts at: 3912 3912
Size: 6313
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5427 5611 1305
Size: 4122 184 2007
Fragments arranged by size:
4122 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3257 3317 10
Size: 54 1425 343 1425 60 3006
Fragments arranged by size:
3006 1425 1425 343 60 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 3004 3101 4271 4630 4789 518
Size: 648 1006 832 97 1170 359 159 2042
Fragments arranged by size:
2042 1170 1006 832 648 359 159 97
MspA1I CmG'CkG
Cuts at: 1606 2028 2449 2484 3729 4406 5554 5799 1606
Size: 422 421 35 1245 677 1148 245 2120
Fragments arranged by size:
2120 1245 1148 677 422 421 245 35
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4822
Fragments arranged by size:
4822 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6313
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 3671 5216 1132
Size: 97 2442 1545 2229
Fragments arranged by size:
2442 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3212 1348
Size: 1864 4449
PciI A'CATG_T
Cuts at: 5212 5212
Size: 6313
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2141 2218 1108
Size: 1033 77 5203
Fragments arranged by size:
5203 1033 77
PfoI T'CCnGG_A
Cuts at: 2812 3065 2812
Size: 253 6060
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2496 2496
Size: 6313
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6313
PsiI TTA'TAA
Cuts at: 3883 3883
Size: 6313
PspXI vC'TCGA_Gb
Cuts at: 3572 3572
Size: 6313
PstI C_TGCA'G
Cuts at: 830 3093 3665 830
Size: 2263 572 3478
Fragments arranged by size:
3478 2263 572
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
PvuII CAG'CTG
Cuts at: 2449 2484 2449
Size: 35 6278
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SanDI GG'GwC_CC
Cuts at: 2496 2496
Size: 6313
SbfI CC_TGCA'GG
Cuts at: 3665 3665
Size: 6313
ScaI AGT'ACT
Cuts at: 2199 4561 6273 2199
Size: 2362 1712 2239
Fragments arranged by size:
2362 2239 1712
SfcI C'TryA_G
Cuts at: 826 1045 2641 3089 3661 4796 5477 5668 826
Size: 219 1596 448 572 1135 681 191 1471
Fragments arranged by size:
1596 1471 1135 681 572 448 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 3525 3599 3640 1872
Size: 1653 74 41 4545
Fragments arranged by size:
4545 1653 74 41
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2265 3572 4417 5318 5580 820
Size: 197 926 322 1307 845 901 262 1553
Fragments arranged by size:
1553 1307 926 901 845 322 262 197
SnaBI TAC'GTA
Cuts at: 493 4104 493
Size: 3611 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6313
SphI G_CATG'C
Cuts at: 1132 3671 1132
Size: 2539 3774
SspI AAT'ATT
Cuts at: 5 52 4237 5
Size: 47 4185 2081
Fragments arranged by size:
4185 2081 47
StuI AGG'CCT
Cuts at: 1517 2561 3279 1517
Size: 1044 718 4551
Fragments arranged by size:
4551 1044 718
StyI C'CwwG_G
Cuts at: 513 1065 2004 2617 2704 3752 513
Size: 552 939 613 87 1048 3074
Fragments arranged by size:
3074 1048 939 613 552 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2453 4340 4357 4510 4695 5140 325
Size: 1255 873 1887 17 153 185 445 1498
Fragments arranged by size:
1887 1498 1255 873 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2197 4559 6271
Size: 274 80 33 51 1443 220 2362 1712
Cuts at: 6271 96
Size: 138
Fragments arranged by size:
2362 1712 1443 274 220 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2343 2375 2808 2840 3447 3479 6273 6305 2343
Size: 32 433 32 607 32 2794 32 2351
Fragments arranged by size:
2794 2351 607 433 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6313
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2202 2702 2947 2202
Size: 500 245 5568
Fragments arranged by size:
5568 500 245
XhoI C'TCGA_G
Cuts at: 1943 3572 1943
Size: 1629 4684
XmaI C'CCGG_G
Cuts at: 1870 3523 3597 3638 1870
Size: 1653 74 41 4545
Fragments arranged by size:
4545 1653 74 41
XmnI GAAnn'nnTTC
Cuts at: 2096 4442 2096
Size: 2346 3967
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3170 4121 276
Size: 53 83 186 936 1636 951 2468
Fragments arranged by size:
2468 1636 951 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI