Restriction map for
pFN21AE3226
(Circular) (Six-base) MAPSORT of: pFN21AE3226.seq Check: 9274 from: 1 to: 6262
pFN21AE3226 6262 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4072 278
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3605 1937
Size: 1668 4594
Acc65I G'GTAC_C
Cuts at: 3583 3583
Size: 6262
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2196 2211 2409 2935 3080 4325 5709 5977
Size: 564 15 198 526 145 1245 1384 268
Cuts at: 5977 1632
Size: 1917
Fragments arranged by size:
1917 1384 1245 564 526 268 198 145
15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 2714 5161 6138 1203
Size: 1511 2447 977 1327
Fragments arranged by size:
2447 1511 1327 977
AgeI A'CCGG_T
Cuts at: 2443 5093 2443
Size: 2650 3612
AhdI GACnn_n'nnGTC
Cuts at: 4991 4991
Size: 6262
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6262
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 6230
AlwNI CAG_nnn'CTG
Cuts at: 4005 5577 4005
Size: 1572 4690
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 2309 2545 2897 3033 3571 3797 3949 6058
Size: 816 236 352 136 538 226 152 2109
Cuts at: 6058 1493
Size: 1697
Fragments arranged by size:
2109 1697 816 538 352 236 226 152
136
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 3587 1870
Size: 73 93 1551 4545
Fragments arranged by size:
4545 1551 93 73
BamHI G'GATC_C
Cuts at: 3592 3959 3592
Size: 367 5895
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3583 5038 618
Size: 325 217 627 1796 1455 1842
Fragments arranged by size:
1842 1796 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 3581 1391
Size: 646 1544 4072
Fragments arranged by size:
4072 1544 646
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2379 928
Size: 971 480 4811
Fragments arranged by size:
4811 971 480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2396 2430 4453 4487 1903
Size: 34 459 34 2023 34 3678
Fragments arranged by size:
3678 2023 459 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2404 4154 5364 2404
Size: 1750 1210 3302
Fragments arranged by size:
3302 1750 1210
BclI T'GATC_A
Cuts at: 1548 3097 1548
Size: 1549 4713
BfrBI ATG'CAT
Cuts at: 2807 2807
Size: 6262
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4873 136
Size: 107 122 71 631 605 3201 1525
Fragments arranged by size:
3201 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6257 6257
Size: 6262
BlpI GC'TnA_GC
Cuts at: 3679 3679
Size: 6262
Bme1580I G_kGCm'C
Cuts at: 1790 2659 4323 5479 1790
Size: 869 1664 1156 2573
Fragments arranged by size:
2573 1664 1156 869
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3993 4951 442
Size: 416 53 83 399 24 2576 958 1753
Fragments arranged by size:
2576 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2229 3332 4922 1239
Size: 990 1103 1590 2579
Fragments arranged by size:
2579 1590 1103 990
Bpu10I CC'TnA_GC
Cuts at: 2031 2291 2031
Size: 260 6002
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4387 5252 5550 4387
Size: 865 298 5099
Fragments arranged by size:
5099 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3531 4925 882
Size: 617 2032 1394 2219
Fragments arranged by size:
2219 2032 1394 617
BsaAI yAC'GTr
Cuts at: 493 1204 2969 4053 493
Size: 711 1765 1084 2702
Fragments arranged by size:
2702 1765 1084 711
BsaBI GATnn'nnATC
Cuts at: 6256 6256
Size: 6262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4069 4451 5086 275
Size: 53 83 186 936 2536 382 635 1451
Fragments arranged by size:
2536 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2443 4695 5093 5367 5514 5925 2443
Size: 2252 398 274 147 411 2780
Fragments arranged by size:
2780 2252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3305 3335 1178
Size: 30 165 30 1902 30 4105
Fragments arranged by size:
4105 1902 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3378 1186
Size: 2192 4070
BseYI C'CCAG_C
Cuts at: 2060 5465 5961 2060
Size: 3405 496 2361
Fragments arranged by size:
3405 2361 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5689
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3712 4473 5501 1540
Size: 275 186 1711 761 1028 2301
Fragments arranged by size:
2301 1711 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4323 4408 5479 4323
Size: 85 1071 5106
Fragments arranged by size:
5106 1071 85
BsmI GAATG_Cn'
Cuts at: 2680 3024 3773 3866 2680
Size: 344 749 93 5076
Fragments arranged by size:
5076 749 344 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2659 3581 4323 4408 5479 1391
Size: 399 247 622 922 742 85 1071 2174
Fragments arranged by size:
2174 1071 922 742 622 399 247 85
BspEI T'CCGG_A
Cuts at: 5925 5925
Size: 6262
BspHI T'CATG_A
Cuts at: 3196 4151 5881 3196
Size: 955 1730 3577
Fragments arranged by size:
3577 1730 955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3617 844
Size: 2773 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4149 1394
Size: 429 2326 3507
Fragments arranged by size:
3507 2326 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4757 4931 65
Size: 1183 3509 174 1396
Fragments arranged by size:
3509 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2443 4906 5093 1700
Size: 110 633 2463 187 2869
Fragments arranged by size:
2869 2463 633 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6262
BssSI C'ACGA_G
Cuts at: 2415 4322 5334 2415
Size: 1907 1012 3343
Fragments arranged by size:
3343 1907 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6262
BstBI TT'CG_AA
Cuts at: 5066 5066
Size: 6262
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6262
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6262
BstYI r'GATC_y
Cuts at: 1972 2008 3592 3959 4344 4361 5813 5899 5911
Size: 36 1584 367 385 17 1452 86 12
Cuts at: 5911 6257 1972
Size: 346 1977
Fragments arranged by size:
1977 1584 1452 385 367 346 86 36
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2072 513
Size: 552 939 68 4703
Fragments arranged by size:
4703 939 552 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2010 487
Size: 683 56 784 4739
Fragments arranged by size:
4739 784 683 56
BtsI GCAGTG_nn'
Cuts at: 3778 4572 4592 3778
Size: 794 20 5448
Fragments arranged by size:
5448 794 20
ClaI AT'CG_AT
Cuts at: 1554 3270 1554
Size: 1716 4546
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6227
DraI TTT'AAA
Cuts at: 2592 2622 2881 2941 3566 2592
Size: 30 259 60 625 5288
Fragments arranged by size:
5288 625 259 60 30
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5269 809
Size: 507 3953 1802
Fragments arranged by size:
3953 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 3091 3709
Size: 54 1425 50 275 18 303 958 618
Cuts at: 3709 4598 6227 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 958 889 618 303 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3709 1537
Size: 275 1897 4090
Fragments arranged by size:
4090 1897 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3177 3401 4192 1353
Size: 1824 224 791 3423
Fragments arranged by size:
3423 1824 791 224
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4849 5223 5369 387
Size: 1064 138 3260 374 146 1280
Fragments arranged by size:
3260 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2196 2211 2229 2409 2935 3080 3332
Size: 393 564 15 18 180 526 145 252
Cuts at: 3332 4325 4922 5709 5977 1239
Size: 993 597 787 268 1524
Fragments arranged by size:
1524 993 787 597 564 526 393 268
252 180 145 18 15
EcoO109I rG'GnC_Cy
Cuts at: 2057 2057
Size: 6262
EcoRI G'AATT_C
Cuts at: 3033 3571 3949 3033
Size: 538 378 5346
Fragments arranged by size:
5346 538 378
EcoRV GAT'ATC
Cuts at: 2488 2488
Size: 6262
FspI TGC'GCA
Cuts at: 4768 4768
Size: 6262
HaeII r_GCGC'y
Cuts at: 1678 5409 1678
Size: 3731 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4903 4935 4977 5009 1373
Size: 32 135 32 3331 32 42 32 2626
Fragments arranged by size:
3331 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2401 2740 3606 3852 669
Size: 988 281 463 339 866 246 3079
Fragments arranged by size:
3079 988 866 463 339 281 246
HindIII A'AGCT_T
Cuts at: 748 3462 748
Size: 2714 3548
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KpnI G_GTAC'C
Cuts at: 3587 3587
Size: 6262
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2733 3068 5376 5560 1305
Size: 1428 335 2308 184 2007
Fragments arranged by size:
2308 2007 1428 335 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 3093 10
Size: 54 1425 343 303 958 3179
Fragments arranged by size:
3179 1425 958 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2367 2808 2981 4220 4579 4738 518
Size: 648 1201 441 173 1239 359 159 2042
Fragments arranged by size:
2042 1239 1201 648 441 359 173 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2131 3678 4355 5503 5748 1606
Size: 442 83 1547 677 1148 245 2120
Fragments arranged by size:
2120 1547 1148 677 442 245 83
NcoI C'CATG_G
Cuts at: 513 1065 2004 2072 513
Size: 552 939 68 4703
Fragments arranged by size:
4703 939 552 68
NdeI CA'TA_TG
Cuts at: 387 2809 387
Size: 2422 3840
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NsiI A_TGCA'T
Cuts at: 2809 2809
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 2633 2986 3241 3251 3620 5165 1132
Size: 97 1404 353 255 10 369 1545 2229
Fragments arranged by size:
2229 1545 1404 369 353 255 97 10
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6262
PciI A'CATG_T
Cuts at: 5161 5161
Size: 6262
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6262
PfoI T'CCnGG_A
Cuts at: 3324 3324
Size: 6262
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 3378 3410 4331 4363 5844 5876 3305
Size: 32 41 32 921 32 1481 32 3691
Fragments arranged by size:
3691 1481 921 41 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2057 2057
Size: 6262
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6262
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PstI C_TGCA'G
Cuts at: 830 3335 3614 830
Size: 2505 279 3478
Fragments arranged by size:
3478 2505 279
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
PvuII CAG'CTG
Cuts at: 2131 2131
Size: 6262
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SanDI GG'GwC_CC
Cuts at: 2057 2057
Size: 6262
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 4510 6222 4510
Size: 1712 4550
SfcI C'TryA_G
Cuts at: 826 1045 2255 2428 2685 2856 3331 3610 4745
Size: 219 1210 173 257 171 475 279 1135
Cuts at: 4745 5426 5617 826
Size: 681 191 1471
Fragments arranged by size:
1471 1210 1135 681 475 279 257 219
191 173 171
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 2038 3589 1872
Size: 166 1551 4545
Fragments arranged by size:
4545 1551 166
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4366 5267 5529 820
Size: 197 926 2423 901 262 1553
Fragments arranged by size:
2423 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6262
SphI G_CATG'C
Cuts at: 1132 3251 3620 1132
Size: 2119 369 3774
Fragments arranged by size:
3774 2119 369
SspI AAT'ATT
Cuts at: 5 52 4186 5
Size: 47 4134 2081
Fragments arranged by size:
4134 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6262
StyI C'CwwG_G
Cuts at: 513 1065 2004 2072 3701 513
Size: 552 939 68 1629 3074
Fragments arranged by size:
3074 1629 939 552 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4289 4306 4459 4644 5089 325
Size: 1255 2709 17 153 185 445 1498
Fragments arranged by size:
2709 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2777 3052 4508
Size: 274 80 33 51 1443 800 275 1456
Cuts at: 4508 6220 96
Size: 1712 138
Fragments arranged by size:
1712 1456 1443 800 275 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6222 6254 6222
Size: 32 6230
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6262
XbaI T'CTAG_A
Cuts at: 3598 3598
Size: 6262
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 2036 3587 1870
Size: 166 1551 4545
Fragments arranged by size:
4545 1551 166
XmnI GAAnn'nnTTC
Cuts at: 4391 4391
Size: 6262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4070 276
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI