Restriction map for
pFN21AE3225
(Circular) (Six-base) MAPSORT of: pFN21AE3225.seq Check: 5764 from: 1 to: 6256
pFN21AE3225 6256 bp
With 182 enzymes: SgfI *
March 28, 2012 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2240 2240
Size: 6256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3599 1937
Size: 1662 4594
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 2888 4383 4756 1563
Size: 1325 1495 373 3063
Fragments arranged by size:
3063 1495 1325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3035 3570 4319 5703 5971 1632
Size: 1403 535 749 1384 268 1917
Fragments arranged by size:
1917 1403 1384 749 535 268
AfeI AGC'GCT
Cuts at: 2060 2060
Size: 6256
AflII C'TTAA_G
Cuts at: 820 1017 2443 820
Size: 197 1426 4633
Fragments arranged by size:
4633 1426 197
AflIII A'CryG_T
Cuts at: 1203 5155 6132 1203
Size: 3952 977 1327
Fragments arranged by size:
3952 1327 977
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 3362 4985 3362
Size: 1623 4633
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 2986 3424 3999 5571 2986
Size: 438 575 1572 3671
Fragments arranged by size:
3671 1572 575 438
ApaI G_GGCC'C
Cuts at: 3193 3193
Size: 6256
ApaLI G'TGCA_C
Cuts at: 4313 5469 4313
Size: 1156 5100
ApoI r'AATT_y
Cuts at: 1493 3565 3791 3943 6052 1493
Size: 2072 226 152 2109 1697
Fragments arranged by size:
2109 2072 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2547 3581 1870
Size: 73 73 531 1034 4545
Fragments arranged by size:
4545 1034 531 73 73
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3218 3577 5032 618
Size: 325 217 627 1431 359 1455 1842
Fragments arranged by size:
1842 1455 1431 627 359 325 217
BanII G_rGCy'C
Cuts at: 1391 2015 2334 3163 3193 3575 1391
Size: 624 319 829 30 382 4072
Fragments arranged by size:
4072 829 624 382 319 30
BbeI G_GCGC'C
Cuts at: 3222 3222
Size: 6256
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2689 928
Size: 971 790 4495
Fragments arranged by size:
4495 971 790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4447 4481 1903
Size: 34 50 34 2426 34 3678
Fragments arranged by size:
3678 2426 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 2048 4867
Size: 107 122 71 631 605 355 21 2819
Cuts at: 4867 136
Size: 1525
Fragments arranged by size:
2819 1525 631 605 355 122 107 71
21
BglII A'GATC_T
Cuts at: 2463 6251 2463
Size: 3788 2468
BlpI GC'TnA_GC
Cuts at: 2009 2987 3673 2009
Size: 978 686 4592
Fragments arranged by size:
4592 978 686
Bme1580I G_kGCm'C
Cuts at: 1790 3193 4317 5473 1790
Size: 1403 1124 1156 2573
Fragments arranged by size:
2573 1403 1156 1124
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2116 2302 3269
Size: 416 53 83 399 24 699 186 967
Cuts at: 3269 3987 4945 442
Size: 718 958 1753
Fragments arranged by size:
1753 967 958 718 699 416 399 186
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2353 2320
Size: 33 6223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2299 2600 3158 3463 4916 1239
Size: 1060 301 558 305 1453 2579
Fragments arranged by size:
2579 1453 1060 558 305 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4381 5246 5544 4381
Size: 865 298 5093
Fragments arranged by size:
5093 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2529 3436 4919 882
Size: 617 1030 907 1483 2219
Fragments arranged by size:
2219 1483 1030 907 617
BsaAI yAC'GTr
Cuts at: 493 1204 3031 4047 493
Size: 711 1827 1016 2702
Fragments arranged by size:
2702 1827 1016 711
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3219 4063 4445 5080
Size: 53 83 186 936 1686 844 382 635
Cuts at: 5080 275
Size: 1451
Fragments arranged by size:
1686 1451 936 844 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4689 5087 5361 5508 5919 4689
Size: 398 274 147 411 5026
Fragments arranged by size:
5026 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2573 2603 3278 3281 3308
Size: 30 165 30 1170 30 675 3 27
Cuts at: 3308 3311 3458 3488 3530 3560 1178
Size: 3 147 30 42 30 3874
Fragments arranged by size:
3874 1170 675 165 147 42 30 30
30 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3286 1186
Size: 2100 4156
BseYI C'CCAG_C
Cuts at: 5459 5955 5459
Size: 496 5760
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3053 1163
Size: 573 1317 4366
Fragments arranged by size:
4366 1317 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 2082 3706 4467 5495 1540
Size: 275 186 42 39 1624 761 1028 2301
Fragments arranged by size:
2301 1624 1028 761 275 186 42 39
BsiHKAI G_wGCw'C
Cuts at: 2015 3030 4317 4402 5473 2015
Size: 1015 1287 85 1071 2798
Fragments arranged by size:
2798 1287 1071 1015 85
BsmI GAATG_Cn'
Cuts at: 2572 3767 3860 2572
Size: 1195 93 4968
Fragments arranged by size:
4968 1195 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2334 3030 3163 3193 3575 4317
Size: 399 225 319 696 133 30 382 742
Cuts at: 4317 4402 5473 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 696 399 382 319 225
133 85 30
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 2097 2238 4145 5875 2097
Size: 141 1907 1730 2478
Fragments arranged by size:
2478 1907 1730 141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2240 2624 3498 3611 844
Size: 1396 384 874 113 3489
Fragments arranged by size:
3489 1396 874 384 113
BsrBI CCG'CTC
Cuts at: 1394 1823 4143 1394
Size: 429 2320 3507
Fragments arranged by size:
3507 2320 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4751 4925 65
Size: 1183 3503 174 1396
Fragments arranged by size:
3503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4900 5087 1700
Size: 110 3090 187 2869
Fragments arranged by size:
3090 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2451 2864 96
Size: 2355 413 3488
Fragments arranged by size:
3488 2355 413
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 2631 2786 1184
Size: 1447 155 4654
Fragments arranged by size:
4654 1447 155
BstXI CCAn_nnnn'nTGG
Cuts at: 3080 3080
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 2463 3586 3953 4338 4355 5807 5893 5905
Size: 491 1123 367 385 17 1452 86 12
Cuts at: 5905 6251 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1123 491 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 3001 3337 513
Size: 552 972 964 336 3432
Fragments arranged by size:
3432 972 964 552 336
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2045 487
Size: 683 56 819 4698
Fragments arranged by size:
4698 819 683 56
BtsI GCAGTG_nn'
Cuts at: 2806 3772 4566 4586 2806
Size: 966 794 20 4476
Fragments arranged by size:
4476 966 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3560 3560
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2079 3703
Size: 54 1425 50 275 18 210 39 1624
Cuts at: 3703 4592 6221 8
Size: 889 1629 43
Fragments arranged by size:
1629 1624 1425 889 275 210 54 50
43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2040 2079 3703 1537
Size: 275 228 39 1624 4090
Fragments arranged by size:
4090 1624 275 228 39
EarI CTCTTCn'nnn_
Cuts at: 1353 2289 3314 4186 1353
Size: 936 1025 872 3423
Fragments arranged by size:
3423 1025 936 872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4843 5217 5363 387
Size: 1064 138 3254 374 146 1280
Fragments arranged by size:
3254 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2013 2013
Size: 6256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2299 2600 3035 3158 3463 3570 4319
Size: 393 667 301 435 123 305 107 749
Cuts at: 4319 4916 5703 5971 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 749 667 597 435 393 305
301 268 123 107
EcoNI CCTnn'n_nnAGG
Cuts at: 3464 3464
Size: 6256
EcoO109I rG'GnC_Cy
Cuts at: 2165 3128 3189 3190 3469 2165
Size: 963 61 1 279 4952
Fragments arranged by size:
4952 963 279 61 1
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
FspI TGC'GCA
Cuts at: 4762 4762
Size: 6256
HaeII r_GCGC'y
Cuts at: 1678 2062 3222 3257 3486 5403 1678
Size: 384 1160 35 229 1917 2531
Fragments arranged by size:
2531 1917 1160 384 229 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2079 2111 2448 2480 2573
Size: 32 135 32 507 32 337 32 93
Cuts at: 2573 2605 3278 3310 3335 3348 3367 3380 3458
Size: 32 673 32 25 13 19 13 78
Cuts at: 3458 3490 3530 3562 4897 4929 4971 5003 1373
Size: 32 40 32 1335 32 42 32 2626
Fragments arranged by size:
2626 1335 673 507 337 135 93 78
42 40 32 32 32 32 32 32
32 32 32 32 25 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3098 3600 3846 669
Size: 988 281 1160 502 246 3079
Fragments arranged by size:
3079 1160 988 502 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6256
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KasI G'GCGC_C
Cuts at: 3218 3218
Size: 6256
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2415 5370 5554 1305
Size: 1110 2955 184 2007
Fragments arranged by size:
2955 2007 1110 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4434
Fragments arranged by size:
4434 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4214 4573 4732 518
Size: 648 3048 359 159 2042
Fragments arranged by size:
3048 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2983 3672 4349 5497 5742 1606
Size: 433 944 689 677 1148 245 2120
Fragments arranged by size:
2120 1148 944 689 677 433 245
NarI GG'CG_CC
Cuts at: 3219 3219
Size: 6256
NcoI C'CATG_G
Cuts at: 513 1065 3337 513
Size: 552 2272 3432
Fragments arranged by size:
3432 2272 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 3349 3614 5159 1132
Size: 97 2120 265 1545 2229
Fragments arranged by size:
2229 2120 1545 265 97
PasI CC'CwG_GG
Cuts at: 1348 3125 1348
Size: 1777 4479
PciI A'CATG_T
Cuts at: 5155 5155
Size: 6256
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2655 1108
Size: 1547 4709
PfoI T'CCnGG_A
Cuts at: 3515 3515
Size: 6256
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PmlI CAC'GTG
Cuts at: 3031 3031
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3389 3421 4325 4357 5838 5870 3389
Size: 32 904 32 1481 32 3775
Fragments arranged by size:
3775 1481 904 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2165 3469 2165
Size: 1304 4952
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PspOMI G'GGCC_C
Cuts at: 3189 3189
Size: 6256
PstI C_TGCA'G
Cuts at: 830 3147 3465 3608 830
Size: 2317 318 143 3478
Fragments arranged by size:
3478 2317 318 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 6256
SacI G_AGCT'C
Cuts at: 2015 2015
Size: 6256
SacII CC_GC'GG
Cuts at: 2040 2040
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3598 1936
Size: 1662 4594
SanDI GG'GwC_CC
Cuts at: 2165 2165
Size: 6256
SbfI CC_TGCA'GG
Cuts at: 3465 3608 3465
Size: 143 6113
ScaI AGT'ACT
Cuts at: 2312 4504 6216 2312
Size: 2192 1712 2352
Fragments arranged by size:
2352 2192 1712
SfcI C'TryA_G
Cuts at: 826 1045 2978 3143 3289 3461 3604 4739 5420
Size: 219 1933 165 146 172 143 1135 681
Cuts at: 5420 5611 826
Size: 191 1471
Fragments arranged by size:
1933 1471 1135 681 219 191 172 165
146 143
SfiI GGCCn_nnn'nGGCC
Cuts at: 2048 2048
Size: 6256
SfoI GGC'GCC
Cuts at: 3220 3220
Size: 6256
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 2018 3583 1872
Size: 146 1565 4545
Fragments arranged by size:
4545 1565 146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2443 4360 5261 5523 820
Size: 197 926 500 1917 901 262 1553
Fragments arranged by size:
1917 1553 926 901 500 262 197
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 2690 152
Size: 2538 3718
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 2259 1517
Size: 742 5514
StyI C'CwwG_G
Cuts at: 513 1065 2880 3079 3337 3695 513
Size: 552 1815 199 258 358 3074
Fragments arranged by size:
3074 1815 552 358 258 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2805 4283 4300 4453 4638 5083 325
Size: 1255 1225 1478 17 153 185 445 1498
Fragments arranged by size:
1498 1478 1255 1225 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 2451 2864
Size: 274 80 33 51 1443 333 141 413
Cuts at: 2864 4502 6214 96
Size: 1638 1712 138
Fragments arranged by size:
1712 1638 1443 413 333 274 141 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2630 2662 2837 2869 6216 6248 2630
Size: 32 175 32 3347 32 2638
Fragments arranged by size:
3347 2638 175 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3443 1316
Size: 2127 4129
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2778 2778
Size: 6256
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6256
XmaI C'CCGG_G
Cuts at: 1870 2016 3581 1870
Size: 146 1565 4545
Fragments arranged by size:
4545 1565 146
XmnI GAAnn'nnTTC
Cuts at: 4385 4385
Size: 6256
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspXI PsrI PvuII
SapI SexAI SrfI SwaI