Restriction map for
pFN21AE3223
(Circular) (Six-base) MAPSORT of: pFN21AE3223.seq Check: 5933 from: 1 to: 6223
pFN21AE3223 6223 bp
With 182 enzymes: SgfI *
March 28, 2012 12:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2659 2776 2659
Size: 117 6106
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4033 278
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3566 1937
Size: 1629 4594
Acc65I G'GTAC_C
Cuts at: 3544 3544
Size: 6223
AclI AA'CG_TT
Cuts at: 1563 4350 4723 1563
Size: 2787 373 3063
Fragments arranged by size:
3063 2787 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 3233 3347 4286 5670 5938 1632
Size: 779 822 114 939 1384 268 1917
Fragments arranged by size:
1917 1384 939 822 779 268 114
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6026
AflIII A'CryG_T
Cuts at: 1203 2622 5122 6099 1203
Size: 1419 2500 977 1327
Fragments arranged by size:
2500 1419 1327 977
AgeI A'CCGG_T
Cuts at: 5054 5054
Size: 6223
AhdI GACnn_n'nnGTC
Cuts at: 4952 4952
Size: 6223
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6223
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6223
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2977 3009 2977
Size: 32 6191
AlwNI CAG_nnn'CTG
Cuts at: 2101 2176 2419 2465 2605 2837 3343 3457 3966
Size: 75 243 46 140 232 506 114 509
Cuts at: 3966 5538 2101
Size: 1572 2786
Fragments arranged by size:
2786 1572 509 506 243 232 140 114
75 46
ApaI G_GGCC'C
Cuts at: 3017 3017
Size: 6223
ApaLI G'TGCA_C
Cuts at: 4280 5436 4280
Size: 1156 5067
ApoI r'AATT_y
Cuts at: 1493 3308 3532 3758 3910 6019 1493
Size: 1815 224 226 152 2109 1697
Fragments arranged by size:
2109 1815 1697 226 224 152
AseI AT'TA_AT
Cuts at: 160 1032 4777 160
Size: 872 3745 1606
Fragments arranged by size:
3745 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
AvaI C'yCGr_G
Cuts at: 1870 1943 2768 3548 1870
Size: 73 825 780 4545
Fragments arranged by size:
4545 825 780 73
BamHI G'GATC_C
Cuts at: 2914 3553 3920 2914
Size: 639 367 5217
Fragments arranged by size:
5217 639 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3492 3544 4999 618
Size: 325 217 627 1705 52 1455 1842
Fragments arranged by size:
1842 1705 1455 627 325 217 52
BanII G_rGCy'C
Cuts at: 1391 2660 2745 3017 3075 3542 1391
Size: 1269 85 272 58 467 4072
Fragments arranged by size:
4072 1269 467 272 85 58
BbeI G_GCGC'C
Cuts at: 3496 3496
Size: 6223
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3239 3443 928
Size: 971 1340 204 3708
Fragments arranged by size:
3708 1340 971 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2035 2069 2936 2970 4414 4448 1903
Size: 34 98 34 867 34 1444 34 3678
Fragments arranged by size:
3678 1444 867 98 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4115 5325 4115
Size: 1210 5013
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6223
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4834 136
Size: 107 122 71 631 605 3162 1525
Fragments arranged by size:
3162 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3087 6218 3087
Size: 3131 3092
BlpI GC'TnA_GC
Cuts at: 2595 3640 2595
Size: 1045 5178
Bme1580I G_kGCm'C
Cuts at: 1790 2028 3017 4284 5440 1790
Size: 238 989 1267 1156 2573
Fragments arranged by size:
2573 1267 1156 989 238
BmgBI CAC'GTC
Cuts at: 1318 2623 1318
Size: 1305 4918
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2818 2893 2947
Size: 416 53 83 399 24 1401 75 54
Cuts at: 2947 3954 4912 442
Size: 1007 958 1753
Fragments arranged by size:
1753 1401 1007 958 416 399 83 75
54 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 6223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2940 2979 3147 3239 3299 3428 3452 4883
Size: 1701 39 168 92 60 129 24 1431
Cuts at: 4883 1239
Size: 2579
Fragments arranged by size:
2579 1701 1431 168 129 92 60 39
24
Bpu10I CC'TnA_GC
Cuts at: 2781 3414 2781
Size: 633 5590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3104 3410 4348 5213 5511 3104
Size: 306 938 865 298 3816
Fragments arranged by size:
3816 938 865 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3041 4886 882
Size: 617 1542 1845 2219
Fragments arranged by size:
2219 1845 1542 617
BsaAI yAC'GTr
Cuts at: 493 1204 2023 4014 493
Size: 711 819 1991 2702
Fragments arranged by size:
2702 1991 819 711
BsaBI GATnn'nnATC
Cuts at: 6217 6217
Size: 6223
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2633 3493 4030 4412
Size: 53 83 186 936 1100 860 537 382
Cuts at: 4412 5047 275
Size: 635 1451
Fragments arranged by size:
1451 1100 936 860 635 537 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4656 5054 5328 5475 5886 4656
Size: 398 274 147 411 4993
Fragments arranged by size:
4993 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 2977 3007 1178
Size: 30 165 30 1032 30 512 30 4394
Fragments arranged by size:
4394 1032 512 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2459 2462 2547 2786 3137 3407 1186
Size: 1273 3 85 239 351 270 4002
Fragments arranged by size:
4002 1273 351 270 239 85 3
BseYI C'CCAG_C
Cuts at: 2169 3336 5426 5922 2169
Size: 1167 2090 496 2470
Fragments arranged by size:
2470 2090 1167 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2714 3389 1163
Size: 573 978 675 3997
Fragments arranged by size:
3997 978 675 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3673 4434 5462 1540
Size: 275 186 1672 761 1028 2301
Fragments arranged by size:
2301 1672 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3075 4284 4369 5440 3075
Size: 1209 85 1071 3858
Fragments arranged by size:
3858 1209 1071 85
BsmI GAATG_Cn'
Cuts at: 3734 3827 3734
Size: 93 6130
BsmBI CGTCTCn'nnnn_
Cuts at: 2937 2937
Size: 6223
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2660 2745 3017 3075 3542 4284
Size: 399 238 632 85 272 58 467 742
Cuts at: 4284 4369 5440 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 632 467 399 272 238
85 85 58
BspEI T'CCGG_A
Cuts at: 5886 5886
Size: 6223
BspHI T'CATG_A
Cuts at: 4112 5842 4112
Size: 1730 4493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2568 2659 2776 3432 3578 844
Size: 1724 91 117 656 146 3489
Fragments arranged by size:
3489 1724 656 146 117 91
BsrBI CCG'CTC
Cuts at: 1394 1823 4110 1394
Size: 429 2287 3507
Fragments arranged by size:
3507 2287 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4718 4892 65
Size: 1183 3470 174 1396
Fragments arranged by size:
3470 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4867 5054 1700
Size: 110 3057 187 2869
Fragments arranged by size:
3057 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6223
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6223
BssSI C'ACGA_G
Cuts at: 4283 5295 4283
Size: 1012 5211
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2465 2674 2798 794
Size: 1671 209 124 4219
Fragments arranged by size:
4219 1671 209 124
BstBI TT'CG_AA
Cuts at: 5027 5027
Size: 6223
BstEII G'GTnAC_C
Cuts at: 1184 3199 1184
Size: 2015 4208
BstYI r'GATC_y
Cuts at: 1972 2379 2914 3087 3223 3553 3920 4305 4322
Size: 407 535 173 136 330 367 385 17
Cuts at: 4322 5774 5860 5872 6218 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 535 407 385 367 346 330
173 136 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3204 513
Size: 552 939 1200 3532
Fragments arranged by size:
3532 1200 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5484
Fragments arranged by size:
5484 683 56
BtsI GCAGTG_nn'
Cuts at: 2077 2203 2838 3029 3114 3739 4533 4553 2077
Size: 126 635 191 85 625 794 20 3747
Fragments arranged by size:
3747 794 635 625 191 126 85 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3273 3308 529
Size: 35 2709 35 3444
Fragments arranged by size:
3444 2709 35 35
DraI TTT'AAA
Cuts at: 3527 3527
Size: 6223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5230 809
Size: 507 3914 1802
Fragments arranged by size:
3914 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2263 3670 4559
Size: 54 1425 50 275 18 433 1407 889
Cuts at: 4559 6188 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1407 889 433 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3670 1537
Size: 275 1858 4090
Fragments arranged by size:
4090 1858 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 2278 4153 1353
Size: 801 124 1875 3423
Fragments arranged by size:
3423 1875 801 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4810 5184 5330 387
Size: 1064 138 3221 374 146 1280
Fragments arranged by size:
3221 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3073 3073
Size: 6223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2411 2940 2979 3147 3233 3239 3299
Size: 393 779 529 39 168 86 6 60
Cuts at: 3299 3347 3428 3452 4286 4883 5670 5938 1239
Size: 48 81 24 834 597 787 268 1524
Fragments arranged by size:
1524 834 787 779 597 529 393 268
168 86 81 60 48 39 24 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2552 3283 2552
Size: 731 5492
EcoO109I rG'GnC_Cy
Cuts at: 2184 2557 3013 2184
Size: 373 456 5394
Fragments arranged by size:
5394 456 373
EcoRI G'AATT_C
Cuts at: 3308 3532 3910 3308
Size: 224 378 5621
Fragments arranged by size:
5621 378 224
FspI TGC'GCA
Cuts at: 4729 4729
Size: 6223
HaeII r_GCGC'y
Cuts at: 1678 3496 5370 1678
Size: 1818 1874 2531
Fragments arranged by size:
2531 1874 1818
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2293 2325 2751 2783 2819
Size: 32 135 32 721 32 426 32 36
Cuts at: 2819 2851 3040 3072 3122 3154 4864 4896 4938
Size: 32 189 32 50 32 1710 32 42
Cuts at: 4938 4970 1373
Size: 32 2626
Fragments arranged by size:
2626 1710 721 426 189 135 50 42
36 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2371 3567 3813 669
Size: 988 281 433 1196 246 3079
Fragments arranged by size:
3079 1196 988 433 281 246
HindIII A'AGCT_T
Cuts at: 748 3236 748
Size: 2488 3735
HpaI GTT'AAC
Cuts at: 3813 3813
Size: 6223
KasI G'GCGC_C
Cuts at: 3492 3492
Size: 6223
KpnI G_GTAC'C
Cuts at: 3548 3548
Size: 6223
MfeI C'AATT_G
Cuts at: 3822 3822
Size: 6223
MluI A'CGCG_T
Cuts at: 6099 6099
Size: 6223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5337 5521 1305
Size: 4032 184 2007
Fragments arranged by size:
4032 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4401
Fragments arranged by size:
4401 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 4181 4540 4699 518
Size: 648 1455 1560 359 159 2042
Fragments arranged by size:
2042 1560 1455 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2173 2281 2419 2572 2591 3157 3253 3340
Size: 567 108 138 153 19 566 96 87
Cuts at: 3340 3489 3639 4316 5464 5709 1606
Size: 149 150 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 567 566 245 153 150
149 138 108 96 87 19
NarI GG'CG_CC
Cuts at: 3493 3493
Size: 6223
NcoI C'CATG_G
Cuts at: 513 1065 2004 3204 513
Size: 552 939 1200 3532
Fragments arranged by size:
3532 1200 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6223
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6223
NotI GC'GGCC_GC
Cuts at: 3670 3670
Size: 6223
NspI r_CATG'y
Cuts at: 1132 1229 2149 3581 5126 1132
Size: 97 920 1432 1545 2229
Fragments arranged by size:
2229 1545 1432 920 97
PasI CC'CwG_GG
Cuts at: 1348 2605 1348
Size: 1257 4966
PciI A'CATG_T
Cuts at: 5122 5122
Size: 6223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3343 1108
Size: 2235 3988
PfoI T'CCnGG_A
Cuts at: 2139 2217 2895 2917 2139
Size: 78 678 22 5445
Fragments arranged by size:
5445 678 78 22
PmeI GTTT'AAAC
Cuts at: 3527 3527
Size: 6223
PmlI CAC'GTG
Cuts at: 2023 2023
Size: 6223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2977 3009 4292 4324 5805 5837 2977
Size: 32 1283 32 1481 32 3363
Fragments arranged by size:
3363 1481 1283 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2184 2557 2184
Size: 373 5850
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6223
PsiI TTA'TAA
Cuts at: 3793 3793
Size: 6223
PspOMI G'GGCC_C
Cuts at: 3013 3013
Size: 6223
PspXI vC'TCGA_Gb
Cuts at: 2768 2768
Size: 6223
PstI C_TGCA'G
Cuts at: 830 2084 2424 3177 3453 3575 830
Size: 1254 340 753 276 122 3478
Fragments arranged by size:
3478 1254 753 340 276 122
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6223
PvuII CAG'CTG
Cuts at: 2173 2419 2572 3157 3253 3340 2173
Size: 246 153 585 96 87 5056
Fragments arranged by size:
5056 585 246 153 96 87
SacI G_AGCT'C
Cuts at: 3075 3075
Size: 6223
SalI G'TCGA_C
Cuts at: 1936 3565 1936
Size: 1629 4594
SbfI CC_TGCA'GG
Cuts at: 3177 3575 3177
Size: 398 5825
ScaI AGT'ACT
Cuts at: 4471 6183 4471
Size: 1712 4511
SfcI C'TryA_G
Cuts at: 826 1045 2080 2420 3173 3449 3571 4706 5387
Size: 219 1035 340 753 276 122 1135 681
Cuts at: 5387 5578 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1035 753 681 340 276 219
191 122
SfoI GGC'GCC
Cuts at: 3494 3494
Size: 6223
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6223
SmaI CCC'GGG
Cuts at: 1872 3550 1872
Size: 1678 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2768 3083 3389 4327 5228 5490
Size: 197 926 825 315 306 938 901 262
Cuts at: 5490 820
Size: 1553
Fragments arranged by size:
1553 938 926 901 825 315 306 262
197
SnaBI TAC'GTA
Cuts at: 493 4014 493
Size: 3521 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6223
SphI G_CATG'C
Cuts at: 1132 3581 1132
Size: 2449 3774
SspI AAT'ATT
Cuts at: 5 52 2257 4147 5
Size: 47 2205 1890 2081
Fragments arranged by size:
2205 2081 1890 47
StuI AGG'CCT
Cuts at: 1517 2243 1517
Size: 726 5497
StyI C'CwwG_G
Cuts at: 513 1065 2004 3204 3284 3441 3662 513
Size: 552 939 1200 80 157 221 3074
Fragments arranged by size:
3074 1200 939 552 221 157 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3312 4250 4267 4420 4605 5050 325
Size: 1255 1732 938 17 153 185 445 1498
Fragments arranged by size:
1732 1498 1255 938 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4469 6181 96
Size: 274 80 33 51 1443 2492 1712 138
Fragments arranged by size:
2492 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2435 2467 6183 6215 2435
Size: 32 3716 32 2443
Fragments arranged by size:
3716 2443 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6223
XbaI T'CTAG_A
Cuts at: 3559 3559
Size: 6223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2177 3193 2177
Size: 1016 5207
XhoI C'TCGA_G
Cuts at: 1943 2768 1943
Size: 825 5398
XmaI C'CCGG_G
Cuts at: 1870 3548 1870
Size: 1678 4545
XmnI GAAnn'nnTTC
Cuts at: 3059 4352 3059
Size: 1293 4930
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4031 276
Size: 53 83 186 936 2497 2468
Fragments arranged by size:
2497 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI