Restriction map for
pFN21AE3221
(Circular) (Six-base) MAPSORT of: pFN21AE3221.seq Check: 2260 from: 1 to: 6208
pFN21AE3221 6208 bp
With 182 enzymes: SgfI *
March 28, 2012 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2144 2960 4271 5655 5923 1632
Size: 512 816 1311 1384 268 1917
Fragments arranged by size:
1917 1384 1311 816 512 268
AfeI AGC'GCT
Cuts at: 2459 2459
Size: 6208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 2379 5107 6084 1203
Size: 1176 2728 977 1327
Fragments arranged by size:
2728 1327 1176 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2974 1184
Size: 1790 4418
AlwNI CAG_nnn'CTG
Cuts at: 2476 3951 5523 2476
Size: 1475 1572 3161
Fragments arranged by size:
3161 1572 1475
ApaI G_GGCC'C
Cuts at: 2038 2038
Size: 6208
ApaLI G'TGCA_C
Cuts at: 2464 2930 3419 4265 5421 2464
Size: 466 489 846 1156 3251
Fragments arranged by size:
3251 1156 846 489 466
ApoI r'AATT_y
Cuts at: 1493 3113 3517 3743 3895 6004 1493
Size: 1620 404 226 152 2109 1697
Fragments arranged by size:
2109 1697 1620 404 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2037 2445 2784 2868 3533 1870
Size: 73 94 408 339 84 665 4545
Fragments arranged by size:
4545 665 408 339 94 84 73
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2023 2589 3529 4984 618
Size: 325 217 627 236 566 940 1455 1842
Fragments arranged by size:
1842 1455 940 627 566 325 236 217
BanII G_rGCy'C
Cuts at: 1391 2038 2220 3246 3417 3527 1391
Size: 647 182 1026 171 110 4072
Fragments arranged by size:
4072 1026 647 182 171 110
BbeI G_GCGC'C
Cuts at: 2027 2027
Size: 6208
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3014 3351 928
Size: 971 1115 337 3785
Fragments arranged by size:
3785 1115 971 337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4399 4433 1903
Size: 34 2462 34 3678
Fragments arranged by size:
3678 2462 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2329 4100 5310 2329
Size: 1771 1210 3227
Fragments arranged by size:
3227 1771 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2246 3061
Size: 107 122 71 631 605 337 237 815
Cuts at: 3061 4819 136
Size: 1758 1525
Fragments arranged by size:
1758 1525 815 631 605 337 237 122
107 71
BglII A'GATC_T
Cuts at: 6203 6203
Size: 6208
BlpI GC'TnA_GC
Cuts at: 2486 2643 3625 2486
Size: 157 982 5069
Fragments arranged by size:
5069 982 157
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2468 2934 3423 4269 5425 1790
Size: 248 430 466 489 846 1156 2573
Fragments arranged by size:
2573 1156 846 489 466 430 248
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3939 4897 442
Size: 416 53 83 399 24 2522 958 1753
Fragments arranged by size:
2522 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2950 2950
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2765 3140 3311 3491 4868 1239
Size: 872 654 375 171 180 1377 2579
Fragments arranged by size:
2579 1377 872 654 375 180 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 3470 4333 5198 5496 2381
Size: 1089 863 865 298 3093
Fragments arranged by size:
3093 1089 865 863 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3251 4871 882
Size: 617 1752 1620 2219
Fragments arranged by size:
2219 1752 1620 617
BsaAI yAC'GTr
Cuts at: 493 1204 2380 3999 493
Size: 711 1176 1619 2702
Fragments arranged by size:
2702 1619 1176 711
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2024 3236 4015 4397
Size: 53 83 186 936 491 1212 779 382
Cuts at: 4397 5032 275
Size: 635 1451
Fragments arranged by size:
1451 1212 936 779 635 491 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3447 4641 5039 5313 5460 5871 3447
Size: 1194 398 274 147 411 3784
Fragments arranged by size:
3784 1194 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2708 2738 1178
Size: 30 165 30 1305 30 4648
Fragments arranged by size:
4648 1305 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 2270 2810 2894 3017 3224 1186
Size: 1018 66 540 84 123 207 4170
Fragments arranged by size:
4170 1018 540 207 123 84 66
BseYI C'CCAG_C
Cuts at: 5411 5907 5411
Size: 496 5712
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2480 2651 3011 3338 1163
Size: 573 744 171 360 327 4033
Fragments arranged by size:
4033 744 573 360 327 171
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 3658 4419 5447 1540
Size: 275 186 1447 210 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 210 186
BsiHKAI G_wGCw'C
Cuts at: 2168 2220 2468 2922 2934 3417 3423 3479 4269
Size: 52 248 454 12 483 6 56 790
Cuts at: 4269 4354 5425 2168
Size: 85 1071 2951
Fragments arranged by size:
2951 1071 790 483 454 248 85 56
52 12 6
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2168 2220 2468 2922 2934 3246
Size: 399 248 130 52 248 454 12 312
Cuts at: 3246 3417 3423 3479 3527 4269 4354 5425 1391
Size: 171 6 56 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 312 248 248
171 130 85 56 52 48 12 6
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 4097 5827 4097
Size: 1730 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2122 2673 3033 3384 3563 844
Size: 1278 551 360 351 179 3489
Fragments arranged by size:
3489 1278 551 360 351 179
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 2056 4095 1394
Size: 429 227 6 2039 3507
Fragments arranged by size:
3507 2039 429 227 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 2802 4703 4877 65
Size: 1183 1554 1901 174 1396
Fragments arranged by size:
1901 1554 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 2883 3348 3357 4852 5039 1700
Size: 110 210 863 465 9 1495 187 2869
Fragments arranged by size:
2869 1495 863 465 210 187 110 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6208
BssHII G'CGCG_C
Cuts at: 1925 3311 1925
Size: 1386 4822
BssSI C'ACGA_G
Cuts at: 2252 3416 3437 4268 5280 2252
Size: 1164 21 831 1012 3180
Fragments arranged by size:
3180 1164 1012 831 21
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2287 794
Size: 1493 4715
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstXI CCAn_nnnn'nTGG
Cuts at: 2774 3019 2774
Size: 245 5963
BstYI r'GATC_y
Cuts at: 1972 2262 2472 3538 3905 4290 4307 5759 5845
Size: 290 210 1066 367 385 17 1452 86
Cuts at: 5845 5857 6203 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1066 385 367 346 290 210
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2178 2521 3018 3493 513
Size: 552 939 174 343 497 475 3228
Fragments arranged by size:
3228 939 552 497 475 343 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5469
Fragments arranged by size:
5469 683 56
BtsI GCAGTG_nn'
Cuts at: 2956 3080 3287 3724 4518 4538 2956
Size: 124 207 437 794 20 4626
Fragments arranged by size:
4626 794 437 207 124 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3475 3510 529
Size: 35 2911 35 3227
Fragments arranged by size:
3227 2911 35 35
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DraIII CAC_nnn'GTG
Cuts at: 2917 2917
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2786 5215 809
Size: 507 1470 2429 1802
Fragments arranged by size:
2429 1802 1470 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2697 3459 3655
Size: 54 1425 50 275 18 867 762 196
Cuts at: 3655 4544 6173 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 867 762 275 196 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3655 1537
Size: 275 1843 4090
Fragments arranged by size:
4090 1843 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2912 2927 4138 1353
Size: 1559 15 1211 3423
Fragments arranged by size:
3423 1559 1211 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3056 4795 5169 5315 387
Size: 1064 138 1467 1739 374 146 1280
Fragments arranged by size:
1739 1467 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2218 3415 2218
Size: 1197 5011
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2144 2765 2960 3140 3311 3491
Size: 393 479 33 621 195 180 171 180
Cuts at: 3491 4271 4868 5655 5923 1239
Size: 780 597 787 268 1524
Fragments arranged by size:
1524 787 780 621 597 479 393 268
195 180 180 171 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2353 3437 2353
Size: 1084 5124
EcoO109I rG'GnC_Cy
Cuts at: 2872 3063 3400 3498 2872
Size: 191 337 98 5582
Fragments arranged by size:
5582 337 191 98
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 2027 2461 3396 5355 1678
Size: 349 434 935 1959 2531
Fragments arranged by size:
2531 1959 935 434 349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 2708 2740 3392
Size: 32 135 32 794 32 310 32 652
Cuts at: 3392 3424 4849 4881 4923 4955 1373
Size: 32 1425 32 42 32 2626
Fragments arranged by size:
2626 1425 794 652 310 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3552 3798 669
Size: 988 281 1614 246 3079
Fragments arranged by size:
3079 1614 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KasI G'GCGC_C
Cuts at: 2023 2023
Size: 6208
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5322 5506 1305
Size: 4017 184 2007
Fragments arranged by size:
4017 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4386
Fragments arranged by size:
4386 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3275 4166 4525 4684 518
Size: 648 2109 891 359 159 2042
Fragments arranged by size:
2109 2042 891 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2284 2711 3070 3364 3624 4301 5449 5694
Size: 678 427 359 294 260 677 1148 245
Cuts at: 5694 1606
Size: 2120
Fragments arranged by size:
2120 1148 678 677 427 359 294 260
245
NaeI GCC'GGC
Cuts at: 2022 2885 2022
Size: 863 5345
NarI GG'CG_CC
Cuts at: 2024 2024
Size: 6208
NcoI C'CATG_G
Cuts at: 513 1065 2004 2178 3018 513
Size: 552 939 174 840 3703
Fragments arranged by size:
3703 939 840 552 174
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NgoMIV G'CCGG_C
Cuts at: 2020 2883 2020
Size: 863 5345
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 2152 2842 3566 5111 1132
Size: 97 923 690 724 1545 2229
Fragments arranged by size:
2229 1545 923 724 690 97
PasI CC'CwG_GG
Cuts at: 1348 3175 1348
Size: 1827 4381
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2687 1108
Size: 1579 4629
PfoI T'CCnGG_A
Cuts at: 2088 3117 3468 2088
Size: 1029 351 4828
Fragments arranged by size:
4828 1029 351
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2872 3400 3498 2872
Size: 528 98 5582
Fragments arranged by size:
5582 528 98
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PspOMI G'GGCC_C
Cuts at: 2034 2034
Size: 6208
PstI C_TGCA'G
Cuts at: 830 2507 3075 3560 830
Size: 1677 568 485 3478
Fragments arranged by size:
3478 1677 568 485
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2284 3364 2284
Size: 1080 5128
SacI G_AGCT'C
Cuts at: 2220 3417 2220
Size: 1197 5011
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SanDI GG'GwC_CC
Cuts at: 2872 3498 2872
Size: 626 5582
SapI GCTCTTCn'nnn_
Cuts at: 2927 2927
Size: 6208
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 2327 4456 6168 2327
Size: 2129 1712 2367
Fragments arranged by size:
2367 2129 1712
SfcI C'TryA_G
Cuts at: 826 1045 2503 3071 3556 4691 5372 5563 826
Size: 219 1458 568 485 1135 681 191 1471
Fragments arranged by size:
1471 1458 1135 681 568 485 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3061 3061
Size: 6208
SfoI GGC'GCC
Cuts at: 2025 2025
Size: 6208
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 2039 2447 2786 2870 3535 1872
Size: 167 408 339 84 665 4545
Fragments arranged by size:
4545 665 408 339 167 84
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2396 3485 4312 5213 5475 820
Size: 197 926 453 1089 827 901 262 1553
Fragments arranged by size:
1553 1089 926 901 827 453 262 197
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SrfI GCCC'GGGC
Cuts at: 2447 2447
Size: 6208
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 2240 2348 1517
Size: 723 108 5377
Fragments arranged by size:
5377 723 108
StyI C'CwwG_G
Cuts at: 513 1065 2004 2140 2178 3012 3018 3647 513
Size: 552 939 136 38 834 6 629 3074
Fragments arranged by size:
3074 939 834 629 552 136 38 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4235 4252 4405 4590 5035 325
Size: 1255 2655 17 153 185 445 1498
Fragments arranged by size:
2655 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 2755 4454
Size: 274 80 33 51 1443 348 430 1699
Cuts at: 4454 6166 96
Size: 1712 138
Fragments arranged by size:
1712 1699 1443 430 348 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2087 2119 6168 6200 2087
Size: 32 4049 32 2095
Fragments arranged by size:
4049 2095 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3172 3172
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 2037 2445 2784 2868 3533 1870
Size: 167 408 339 84 665 4545
Fragments arranged by size:
4545 665 408 339 167 84
XmnI GAAnn'nnTTC
Cuts at: 2240 4337 2240
Size: 2097 4111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SexAI SwaI