Restriction map for
pFN21AE3220
(Circular) (Six-base) MAPSORT of: pFN21AE3220.seq Check: 5085 from: 1 to: 6193
pFN21AE3220 6193 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3536 1937
Size: 1599 4594
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2462 2830 2880 3036 4256 5640 5908 1632
Size: 830 368 50 156 1220 1384 268 1917
Fragments arranged by size:
1917 1384 1220 830 368 268 156 50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 2184 2898 5092 6069 1203
Size: 981 714 2194 977 1327
Fragments arranged by size:
2194 1327 981 977 714
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 4922 4922
Size: 6193
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AlwNI CAG_nnn'CTG
Cuts at: 2242 2853 3936 5508 2242
Size: 611 1083 1572 2927
Fragments arranged by size:
2927 1572 1083 611
ApaLI G'TGCA_C
Cuts at: 4250 5406 4250
Size: 1156 5037
ApoI r'AATT_y
Cuts at: 1493 2674 3502 3728 3880 5989 1493
Size: 1181 828 226 152 2109 1697
Fragments arranged by size:
2109 1697 1181 828 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 3518 1870
Size: 73 1575 4545
Fragments arranged by size:
4545 1575 73
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3514 4969 618
Size: 325 217 627 1727 1455 1842
Fragments arranged by size:
1842 1727 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3512 1391
Size: 2121 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2607 928
Size: 971 708 4514
Fragments arranged by size:
4514 971 708
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2674 4085 5295 2674
Size: 1411 1210 3572
Fragments arranged by size:
3572 1411 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BfrBI ATG'CAT
Cuts at: 3350 3350
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4804 136
Size: 107 122 71 631 605 3132 1525
Fragments arranged by size:
3132 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6188 6188
Size: 6193
BlpI GC'TnA_GC
Cuts at: 3610 3610
Size: 6193
Bme1580I G_kGCm'C
Cuts at: 1790 4254 5410 1790
Size: 2464 1156 2573
Fragments arranged by size:
2573 2464 1156
BmgBI CAC'GTC
Cuts at: 1318 2987 1318
Size: 1669 4524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3145 3924 4882
Size: 416 53 83 399 24 1728 779 958
Cuts at: 4882 442
Size: 1753
Fragments arranged by size:
1753 1728 958 779 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2594 1056
Size: 1538 4655
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3172 3172
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2158 2273 2976 4853 1239
Size: 919 115 703 1877 2579
Fragments arranged by size:
2579 1877 919 703 115
Bpu10I CC'TnA_GC
Cuts at: 2808 3442 2808
Size: 634 5559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2315 3166 3440 4318 5183 5481 2315
Size: 851 274 878 865 298 3027
Fragments arranged by size:
3027 878 865 851 298 274
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4856 882
Size: 617 3357 2219
Fragments arranged by size:
3357 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2185 3112 3984 493
Size: 711 981 927 872 2702
Fragments arranged by size:
2702 981 927 872 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4000 4382 5017 275
Size: 53 83 186 936 2467 382 635 1451
Fragments arranged by size:
2467 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4626 5024 5298 5445 5856 4626
Size: 398 274 147 411 4963
Fragments arranged by size:
4963 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5968
Fragments arranged by size:
5968 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2973 1186
Size: 1787 4406
BseYI C'CCAG_C
Cuts at: 3067 5396 5892 3067
Size: 2329 496 3368
Fragments arranged by size:
3368 2329 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2219 2255 1163
Size: 573 483 36 5101
Fragments arranged by size:
5101 573 483 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4254 4339 5410 4254
Size: 85 1071 5037
Fragments arranged by size:
5037 1071 85
BsmI GAATG_Cn'
Cuts at: 2353 3704 3797 2353
Size: 1351 93 4749
Fragments arranged by size:
4749 1351 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3512 4254 4339 5410 1391
Size: 399 1722 742 85 1071 2174
Fragments arranged by size:
2174 1722 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5856 5856
Size: 6193
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3548 844
Size: 2704 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2166 2215 4080 1394
Size: 429 343 49 1865 3507
Fragments arranged by size:
3507 1865 429 343 49
BsrDI GCAATG_nn'
Cuts at: 65 1248 4688 4862 65
Size: 1183 3440 174 1396
Fragments arranged by size:
3440 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3233 4837 5024 1700
Size: 110 1423 1604 187 2869
Fragments arranged by size:
2869 1604 1423 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 2464 4253 5265 2464
Size: 1789 1012 3392
Fragments arranged by size:
3392 1789 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 794
Size: 1448 4745
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 3044 1184
Size: 1860 4333
BstYI r'GATC_y
Cuts at: 1972 3523 3890 4275 4292 5744 5830 5842 6188
Size: 1551 367 385 17 1452 86 12 346
Cuts at: 6188 1972
Size: 1977
Fragments arranged by size:
1977 1551 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2715 513
Size: 552 1650 3991
Fragments arranged by size:
3991 1650 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5454
Fragments arranged by size:
5454 683 56
BtsI GCAGTG_nn'
Cuts at: 3709 4503 4523 3709
Size: 794 20 5379
Fragments arranged by size:
5379 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2452 2487 529
Size: 35 1888 35 4235
Fragments arranged by size:
4235 1888 35 35
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DraIII CAC_nnn'GTG
Cuts at: 2280 2280
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2788 5200 809
Size: 507 1472 2412 1802
Fragments arranged by size:
2412 1802 1472 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 3640 4529
Size: 54 1425 50 275 18 219 1591 889
Cuts at: 4529 6158 8
Size: 1629 43
Fragments arranged by size:
1629 1591 1425 889 275 219 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2069 2173 2631 2919 4123 1353
Size: 716 104 458 288 1204 3423
Fragments arranged by size:
3423 1204 716 458 288 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4780 5154 5300 387
Size: 1064 138 3191 374 146 1280
Fragments arranged by size:
3191 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2273 2462 2830 2880 2976 3036
Size: 393 526 115 189 368 50 96 60
Cuts at: 3036 4256 4853 5640 5908 1239
Size: 1220 597 787 268 1524
Fragments arranged by size:
1524 1220 787 597 526 393 368 268
189 115 96 60 50
EcoO109I rG'GnC_Cy
Cuts at: 2121 2812 2121
Size: 691 5502
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 5340 1678
Size: 3662 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2023 2055 4834 4866 4908
Size: 32 135 32 451 32 2779 32 42
Cuts at: 4908 4940 1373
Size: 32 2626
Fragments arranged by size:
2779 2626 451 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2430 2904 3537 3783 669
Size: 988 281 492 474 633 246 3079
Fragments arranged by size:
3079 988 633 492 474 281 246
HindIII A'AGCT_T
Cuts at: 748 2291 748
Size: 1543 4650
HpaI GTT'AAC
Cuts at: 2430 2904 3783 2430
Size: 474 879 4840
Fragments arranged by size:
4840 879 474
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2641 3387 3502 5307 5491 1305
Size: 1336 746 115 1805 184 2007
Fragments arranged by size:
2007 1805 1336 746 184 115
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4371
Fragments arranged by size:
4371 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3467 4151 4510 4669 518
Size: 648 2301 684 359 159 2042
Fragments arranged by size:
2301 2042 684 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2411 3609 4286 5434 5679 1606
Size: 805 1198 677 1148 245 2120
Fragments arranged by size:
2120 1198 1148 805 677 245
NaeI GCC'GGC
Cuts at: 3235 3235
Size: 6193
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5641
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NgoMIV G'CCGG_C
Cuts at: 3233 3233
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 2590 1052
Size: 1538 4655
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NsiI A_TGCA'T
Cuts at: 3352 3352
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 3354 3551 5096 1132
Size: 97 2125 197 1545 2229
Fragments arranged by size:
2229 2125 1545 197 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6193
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6193
PfoI T'CCnGG_A
Cuts at: 2307 2307
Size: 6193
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2466 2481 2498 2513 4262 4294 5775 5807 2466
Size: 15 17 15 1749 32 1481 32 2852
Fragments arranged by size:
2852 1749 1481 32 32 17 15 15
PpuMI rG'GwC_Cy
Cuts at: 2121 2812 2121
Size: 691 5502
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 6161
PstI C_TGCA'G
Cuts at: 830 2129 3545 830
Size: 1299 1416 3478
Fragments arranged by size:
3478 1416 1299
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SapI GCTCTTCn'nnn_
Cuts at: 2069 2173 2069
Size: 104 6089
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 2125 2621 2651 2919 3541 4676 5357
Size: 219 1080 496 30 268 622 1135 681
Cuts at: 5357 5548 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1080 681 622 496 268 219
191 30
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2294 3181 3455 4297 5198 5460
Size: 197 926 351 887 274 842 901 262
Cuts at: 5460 820
Size: 1553
Fragments arranged by size:
1553 926 901 887 842 351 274 262
197
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 3062 4117 5
Size: 47 3010 1055 2081
Fragments arranged by size:
3010 2081 1055 47
StuI AGG'CCT
Cuts at: 1517 2936 1517
Size: 1419 4774
StyI C'CwwG_G
Cuts at: 513 1065 3632 513
Size: 552 2567 3074
Fragments arranged by size:
3074 2567 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4220 4237 4390 4575 5020 325
Size: 1255 2640 17 153 185 445 1498
Fragments arranged by size:
2640 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2353 3378 4439
Size: 274 80 33 51 1443 376 1025 1061
Cuts at: 4439 6151 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1061 1025 376 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6153 6185 6153
Size: 32 6161
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6193
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 2570 4322 2570
Size: 1752 4441
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI PacI
PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI