Restriction map for
pFN21AE3217
(Circular) (Six-base) MAPSORT of: pFN21AE3217.seq Check: 2247 from: 1 to: 6187
pFN21AE3217 6187 bp
With 182 enzymes: SgfI *
March 28, 2012 12:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 2489 2700 3001 3085 3169 4250 5634
Size: 467 390 211 301 84 84 1081 1384
Cuts at: 5634 5902 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1081 467 390 301 268 211
84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 5086 6063 1203
Size: 3883 977 1327
Fragments arranged by size:
3883 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6187
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2802 2834 2802
Size: 32 6155
AlwNI CAG_nnn'CTG
Cuts at: 2266 2293 3930 5502 2266
Size: 27 1637 1572 2951
Fragments arranged by size:
2951 1637 1572 27
ApaLI G'TGCA_C
Cuts at: 2246 4244 5400 2246
Size: 1998 1156 3033
Fragments arranged by size:
3033 1998 1156
ApoI r'AATT_y
Cuts at: 1493 2857 2967 3051 3219 3303 3387 3496 3722
Size: 1364 110 84 168 84 84 109 226
Cuts at: 3722 3874 5983 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1364 226 168 152 110 109
84 84 84
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 2190 3512 1870
Size: 73 247 1322 4545
Fragments arranged by size:
4545 1322 247 73
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3508 4963 618
Size: 325 217 627 1721 1455 1842
Fragments arranged by size:
1842 1721 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2945 3029 3120 3197 3365 3506 1391
Size: 1554 84 91 77 168 141 4072
Fragments arranged by size:
4072 1554 168 141 91 84 77
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5216
BbvCI CC'TCA_GC
Cuts at: 2620 2620
Size: 6187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2202 2236 4378 4412 1903
Size: 34 265 34 2142 34 3678
Fragments arranged by size:
3678 2142 265 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4079 5289 4079
Size: 1210 4977
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BfrBI ATG'CAT
Cuts at: 2993 2993
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4798 136
Size: 107 122 71 631 605 3126 1525
Fragments arranged by size:
3126 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 3196 3364 3604 3196
Size: 168 240 5779
Fragments arranged by size:
5779 240 168
Bme1580I G_kGCm'C
Cuts at: 1790 2250 4248 5404 1790
Size: 460 1998 1156 2573
Fragments arranged by size:
2573 1998 1156 460
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3918 4876 442
Size: 416 53 83 399 24 2501 958 1753
Fragments arranged by size:
2501 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2025 2149 2288 2393 2414 2673 2997 3165
Size: 786 124 139 105 21 259 324 168
Cuts at: 3165 3249 3333 3417 4847 1239
Size: 84 84 84 1430 2579
Fragments arranged by size:
2579 1430 786 324 259 168 139 124
105 84 84 84 21
Bpu10I CC'TnA_GC
Cuts at: 2620 2620
Size: 6187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4312 5177 5475 4312
Size: 865 298 5024
Fragments arranged by size:
5024 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2977 4850 882
Size: 617 1478 1873 2219
Fragments arranged by size:
2219 1873 1478 617
BsaAI yAC'GTr
Cuts at: 493 1204 3978 493
Size: 711 2774 2702
Fragments arranged by size:
2774 2702 711
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3994 4376 5011 275
Size: 53 83 186 936 2461 382 635 1451
Fragments arranged by size:
2461 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2162 2192 1178
Size: 30 165 30 759 30 5173
Fragments arranged by size:
5173 759 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 2390 2411 1186
Size: 1138 66 21 4962
Fragments arranged by size:
4962 1138 66 21
BseYI C'CCAG_C
Cuts at: 3375 5390 5886 3375
Size: 2015 496 3676
Fragments arranged by size:
3676 2015 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2516 1163
Size: 573 780 4834
Fragments arranged by size:
4834 780 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3637 4398 5426 1540
Size: 275 186 1636 761 1028 2301
Fragments arranged by size:
2301 1636 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2250 2467 2945 3029 3120 3197 3365 4248 4333
Size: 217 478 84 91 77 168 883 85
Cuts at: 4333 5404 2250
Size: 1071 3033
Fragments arranged by size:
3033 1071 883 478 217 168 91 85
84 77
BsmI GAATG_Cn'
Cuts at: 3698 3791 3698
Size: 93 6094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2467 2945 3029 3120 3197 3365
Size: 399 460 217 478 84 91 77 168
Cuts at: 3365 3506 4248 4333 5404 1391
Size: 141 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 478 460 399 217 168
141 91 85 84 77
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 2830 4076 5806 2830
Size: 1246 1730 3211
Fragments arranged by size:
3211 1730 1246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3542 844
Size: 2698 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4074 1394
Size: 429 2251 3507
Fragments arranged by size:
3507 2251 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4682 4856 65
Size: 1183 3434 174 1396
Fragments arranged by size:
3434 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2205 4831 5018 1700
Size: 110 395 2626 187 2869
Fragments arranged by size:
2869 2626 395 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6187
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 4247 5259 4247
Size: 1012 5175
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6187
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstXI CCAn_nnnn'nTGG
Cuts at: 2026 2108 2026
Size: 82 6105
BstYI r'GATC_y
Cuts at: 1972 2262 3517 3884 4269 4286 5738 5824 5836
Size: 290 1255 367 385 17 1452 86 12
Cuts at: 5836 6182 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1255 385 367 346 290 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2307 2307
Size: 6187
BtgI C'CryG_G
Cuts at: 513 1065 2805 513
Size: 552 1740 3895
Fragments arranged by size:
3895 1740 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5448
Fragments arranged by size:
5448 683 56
BtsI GCAGTG_nn'
Cuts at: 3703 4497 4517 3703
Size: 794 20 5373
Fragments arranged by size:
5373 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2533 2775 5194 809
Size: 507 1217 242 2419 1802
Fragments arranged by size:
2419 1802 1217 507 242
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2339 2425
Size: 54 1425 50 275 18 465 44 86
Cuts at: 2425 3634 4523 6152 8
Size: 1209 889 1629 43
Fragments arranged by size:
1629 1425 1209 889 465 275 86 54
50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3634 1537
Size: 275 1822 4090
Fragments arranged by size:
4090 1822 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4117 1353
Size: 2764 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4774 5148 5294 387
Size: 1064 138 3185 374 146 1280
Fragments arranged by size:
3185 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2943 3027 3118 3195 3363 2943
Size: 84 91 77 168 5767
Fragments arranged by size:
5767 168 91 84 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2025 2099 2149 2288 2393 2414 2489
Size: 393 393 74 50 139 105 21 75
Cuts at: 2489 2673 2700 2997 3001 3085 3165 3169 3249
Size: 184 27 297 4 84 80 4 80
Cuts at: 3249 3333 3417 4250 4847 5634 5902 1239
Size: 84 84 833 597 787 268 1524
Fragments arranged by size:
1524 833 787 597 393 393 297 268
184 139 105 84 84 84 80 80
75 74 50 27 21 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2306 2306
Size: 6187
EcoRI G'AATT_C
Cuts at: 2857 2967 3051 3303 3387 3496 3874 2857
Size: 110 84 252 84 109 378 5170
Fragments arranged by size:
5170 378 252 110 109 84 84
EcoRV GAT'ATC
Cuts at: 2634 2634
Size: 6187
FspI TGC'GCA
Cuts at: 4693 4693
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 5334 1678
Size: 3656 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2512 2544 2944 2976 4828
Size: 32 135 32 940 32 400 32 1852
Cuts at: 4828 4860 4902 4934 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1852 940 400 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2887 3531 3777 669
Size: 988 281 949 644 246 3079
Fragments arranged by size:
3079 988 949 644 281 246
HindIII A'AGCT_T
Cuts at: 748 2319 2685 748
Size: 1571 366 4250
Fragments arranged by size:
4250 1571 366
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2262 5301 5485 1305
Size: 957 3039 184 2007
Fragments arranged by size:
3039 2007 957 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 2427 10
Size: 54 1425 343 465 130 3770
Fragments arranged by size:
3770 1425 465 343 130 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2835 3060 4145 4504 4663 518
Size: 648 1669 225 1085 359 159 2042
Fragments arranged by size:
2042 1669 1085 648 359 225 159
MspA1I CmG'CkG
Cuts at: 1606 3603 4280 5428 5673 1606
Size: 1997 677 1148 245 2120
Fragments arranged by size:
2120 1997 1148 677 245
NaeI GCC'GGC
Cuts at: 2207 2207
Size: 6187
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5635
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NgoMIV G'CCGG_C
Cuts at: 2205 2205
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NsiI A_TGCA'T
Cuts at: 2995 2995
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 2997 3258 3545 5090 1132
Size: 97 1768 261 287 1545 2229
Fragments arranged by size:
2229 1768 1545 287 261 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2348 1108
Size: 1240 4947
PfoI T'CCnGG_A
Cuts at: 2265 2547 2650 2265
Size: 282 103 5802
Fragments arranged by size:
5802 282 103
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6187
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PstI C_TGCA'G
Cuts at: 830 2734 3539 830
Size: 1904 805 3478
Fragments arranged by size:
3478 1904 805
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
SacI G_AGCT'C
Cuts at: 2945 3029 3120 3197 3365 2945
Size: 84 91 77 168 5767
Fragments arranged by size:
5767 168 91 84 77
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 2730 3535 4670 5351 5542 826
Size: 219 1685 805 1135 681 191 1471
Fragments arranged by size:
1685 1471 1135 805 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4291 5192 5454 820
Size: 197 926 2348 901 262 1553
Fragments arranged by size:
2348 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 2930 3182 3453 1517
Size: 1413 252 271 4251
Fragments arranged by size:
4251 1413 271 252
StyI C'CwwG_G
Cuts at: 513 1065 2253 2402 3626 513
Size: 552 1188 149 1224 3074
Fragments arranged by size:
3074 1224 1188 552 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3389 4214 4231 4384 4569 5014 325
Size: 1255 1809 825 17 153 185 445 1498
Fragments arranged by size:
1809 1498 1255 825 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4433 6145 96
Size: 274 80 33 51 1443 2456 1712 138
Fragments arranged by size:
2456 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6147 6179 6147
Size: 32 6155
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2555 2555
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 2058 3182 4316 2058
Size: 1124 1134 3929
Fragments arranged by size:
3929 1134 1124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstZ17I DraIII EcoO109I FalI FseI FspAI KasI
NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI