Restriction map for
pFN21AE3216
(Circular) (Six-base) MAPSORT of: pFN21AE3216.seq Check: 795 from: 1 to: 6184
pFN21AE3216 6184 bp
With 182 enzymes: SgfI *
March 28, 2012 12:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3994 278
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3527 1937
Size: 1590 4594
Acc65I G'GTAC_C
Cuts at: 3196 3505 3196
Size: 309 5875
AclI AA'CG_TT
Cuts at: 1563 4311 4684 1563
Size: 2748 373 3063
Fragments arranged by size:
3063 2748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2670 2934 3501 4247 5631 5899 1632
Size: 1038 264 567 746 1384 268 1917
Fragments arranged by size:
1917 1384 1038 746 567 268 264
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5987
AflIII A'CryG_T
Cuts at: 1203 5083 6060 1203
Size: 3880 977 1327
Fragments arranged by size:
3880 1327 977
AgeI A'CCGG_T
Cuts at: 5015 5015
Size: 6184
AhdI GACnn_n'nnGTC
Cuts at: 4913 4913
Size: 6184
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3368 1184
Size: 2184 4000
AlwNI CAG_nnn'CTG
Cuts at: 2396 2645 3927 5499 2396
Size: 249 1282 1572 3081
Fragments arranged by size:
3081 1572 1282 249
ApaLI G'TGCA_C
Cuts at: 3380 4241 5397 3380
Size: 861 1156 4167
Fragments arranged by size:
4167 1156 861
ApoI r'AATT_y
Cuts at: 1493 3493 3719 3871 5980 1493
Size: 2000 226 152 2109 1697
Fragments arranged by size:
2109 2000 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4738 160
Size: 872 3706 1606
Fragments arranged by size:
3706 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 3509 1870
Size: 73 114 1452 4545
Fragments arranged by size:
4545 1452 114 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3381 3414 3381
Size: 33 6151
BamHI G'GATC_C
Cuts at: 3514 3881 3514
Size: 367 5817
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2629 3196 3505 4960 618
Size: 325 217 627 842 567 309 1455 1842
Fragments arranged by size:
1842 1455 842 627 567 325 309 217
BanII G_rGCy'C
Cuts at: 1391 3503 1391
Size: 2112 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4375 4409 1903
Size: 34 2438 34 3678
Fragments arranged by size:
3678 2438 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4076 5286 4076
Size: 1210 4974
BclI T'GATC_A
Cuts at: 1548 2569 2824 2968 1548
Size: 1021 255 144 4764
Fragments arranged by size:
4764 1021 255 144
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4795 136
Size: 107 122 71 631 605 3123 1525
Fragments arranged by size:
3123 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2173 6179 2173
Size: 4006 2178
BlpI GC'TnA_GC
Cuts at: 2523 3601 2523
Size: 1078 5106
Bme1580I G_kGCm'C
Cuts at: 1790 3384 4245 5401 1790
Size: 1594 861 1156 2573
Fragments arranged by size:
2573 1594 1156 861
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6184
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2359 3306 3915
Size: 416 53 83 399 24 942 947 609
Cuts at: 3915 4873 442
Size: 958 1753
Fragments arranged by size:
1753 958 947 942 609 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2096 2096
Size: 6184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3414 4844 1239
Size: 2175 1430 2579
Fragments arranged by size:
2579 2175 1430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 4309 5174 5472 2030
Size: 2279 865 298 2742
Fragments arranged by size:
2742 2279 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4847 882
Size: 617 3348 2219
Fragments arranged by size:
3348 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3975 493
Size: 711 2771 2702
Fragments arranged by size:
2771 2702 711
BsaBI GATnn'nnATC
Cuts at: 6178 6178
Size: 6184
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3991 4373 5008 275
Size: 53 83 186 936 2458 382 635 1451
Fragments arranged by size:
2458 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4617 5015 5289 5436 5847 4617
Size: 398 274 147 411 4954
Fragments arranged by size:
4954 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5959
Fragments arranged by size:
5959 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2661 3125 3320 1186
Size: 1475 464 195 4050
Fragments arranged by size:
4050 1475 464 195
BseYI C'CCAG_C
Cuts at: 2509 2518 5387 5883 2509
Size: 9 2869 496 2810
Fragments arranged by size:
2869 2810 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2723 3186 3239 3429 1163
Size: 573 987 463 53 190 3918
Fragments arranged by size:
3918 987 573 463 190 53
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3634 4395 5423 1540
Size: 275 186 1633 761 1028 2301
Fragments arranged by size:
2301 1633 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3357 3384 4245 4330 5401 3357
Size: 27 861 85 1071 4140
Fragments arranged by size:
4140 1071 861 85 27
BsmI GAATG_Cn'
Cuts at: 3121 3695 3788 3121
Size: 574 93 5517
Fragments arranged by size:
5517 574 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3357 3384 3503 4245 4330 5401 1391
Size: 399 1567 27 119 742 85 1071 2174
Fragments arranged by size:
2174 1567 1071 742 399 119 85 27
BspEI T'CCGG_A
Cuts at: 5847 5847
Size: 6184
BspHI T'CATG_A
Cuts at: 4073 5803 4073
Size: 1730 4454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2695 3208 3539 844
Size: 1851 513 331 3489
Fragments arranged by size:
3489 1851 513 331
BsrBI CCG'CTC
Cuts at: 1394 1823 4071 1394
Size: 429 2248 3507
Fragments arranged by size:
3507 2248 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4679 4853 65
Size: 1183 3431 174 1396
Fragments arranged by size:
3431 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4828 5015 1700
Size: 110 3018 187 2869
Fragments arranged by size:
3018 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6184
BssSI C'ACGA_G
Cuts at: 3024 4244 5256 3024
Size: 1220 1012 3952
Fragments arranged by size:
3952 1220 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3224 794
Size: 2430 3754
BstBI TT'CG_AA
Cuts at: 4988 4988
Size: 6184
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6184
BstYI r'GATC_y
Cuts at: 1972 2173 3514 3881 4266 4283 5735 5821 5833
Size: 201 1341 367 385 17 1452 86 12
Cuts at: 5833 6179 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1341 385 367 346 201 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2835 3192 2835
Size: 357 5827
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5632
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5445
Fragments arranged by size:
5445 683 56
BtsI GCAGTG_nn'
Cuts at: 3384 3700 4494 4514 3384
Size: 316 794 20 5054
Fragments arranged by size:
5054 794 316 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6149
DraI TTT'AAA
Cuts at: 2253 3488 2253
Size: 1235 4949
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5191 809
Size: 507 3875 1802
Fragments arranged by size:
3875 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3631 4520 6149
Size: 54 1425 50 275 18 1801 889 1629
Cuts at: 6149 8
Size: 43
Fragments arranged by size:
1801 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3631 1537
Size: 275 1819 4090
Fragments arranged by size:
4090 1819 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4114 1353
Size: 2761 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4771 5145 5291 387
Size: 1064 138 3182 374 146 1280
Fragments arranged by size:
3182 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2670 2934 3414 3501 4247 4844 5631
Size: 393 1038 264 480 87 746 597 787
Cuts at: 5631 5899 1239
Size: 268 1524
Fragments arranged by size:
1524 1038 787 746 597 480 393 268
264 87
EcoRI G'AATT_C
Cuts at: 3493 3871 3493
Size: 378 5806
FspI TGC'GCA
Cuts at: 4690 4690
Size: 6184
HaeII r_GCGC'y
Cuts at: 1678 5331 1678
Size: 3653 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2198 2230 2852 2884 4825
Size: 32 135 32 626 32 622 32 1941
Cuts at: 4825 4857 4899 4931 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1941 626 622 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2871 3528 3774 669
Size: 988 281 933 657 246 3079
Fragments arranged by size:
3079 988 933 657 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6184
HpaI GTT'AAC
Cuts at: 3774 3774
Size: 6184
KpnI G_GTAC'C
Cuts at: 3200 3509 3200
Size: 309 5875
MfeI C'AATT_G
Cuts at: 3783 3783
Size: 6184
MluI A'CGCG_T
Cuts at: 6060 6060
Size: 6184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2501 3087 5298 5482 1305
Size: 1196 586 2211 184 2007
Fragments arranged by size:
2211 2007 1196 586 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4362
Fragments arranged by size:
4362 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4142 4501 4660 518
Size: 648 2976 359 159 2042
Fragments arranged by size:
2976 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2483 2513 3479 3600 4277 5425 5670 1606
Size: 877 30 966 121 677 1148 245 2120
Fragments arranged by size:
2120 1148 966 877 677 245 121 30
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5632
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6184
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6184
NotI GC'GGCC_GC
Cuts at: 3631 3631
Size: 6184
NspI r_CATG'y
Cuts at: 1132 1229 3070 3542 5087 1132
Size: 97 1841 472 1545 2229
Fragments arranged by size:
2229 1841 1545 472 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6184
PciI A'CATG_T
Cuts at: 5083 5083
Size: 6184
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3337 1108
Size: 2229 3955
PfoI T'CCnGG_A
Cuts at: 3210 3210
Size: 6184
PmeI GTTT'AAAC
Cuts at: 3488 3488
Size: 6184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4253 4285 5766 5798 4253
Size: 32 1481 32 4639
Fragments arranged by size:
4639 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6184
PsiI TTA'TAA
Cuts at: 3754 3754
Size: 6184
PspXI vC'TCGA_Gb
Cuts at: 2057 2057
Size: 6184
PstI C_TGCA'G
Cuts at: 830 3373 3536 830
Size: 2543 163 3478
Fragments arranged by size:
3478 2543 163
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6184
PvuII CAG'CTG
Cuts at: 2483 2513 3479 2483
Size: 30 966 5188
Fragments arranged by size:
5188 966 30
SalI G'TCGA_C
Cuts at: 1936 3526 1936
Size: 1590 4594
SbfI CC_TGCA'GG
Cuts at: 3536 3536
Size: 6184
ScaI AGT'ACT
Cuts at: 4432 6144 4432
Size: 1712 4472
SfcI C'TryA_G
Cuts at: 826 1045 2274 2310 3042 3081 3369 3532 4667
Size: 219 1229 36 732 39 288 163 1135
Cuts at: 4667 5348 5539 826
Size: 681 191 1471
Fragments arranged by size:
1471 1229 1135 732 681 288 219 191
163 39 36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6184
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6184
SmaI CCC'GGG
Cuts at: 1872 3511 1872
Size: 1639 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 2057 4288 5189 5451 820
Size: 197 926 102 12 2231 901 262 1553
Fragments arranged by size:
2231 1553 926 901 262 197 102 12
SnaBI TAC'GTA
Cuts at: 493 3975 493
Size: 3482 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6184
SphI G_CATG'C
Cuts at: 1132 3542 1132
Size: 2410 3774
SspI AAT'ATT
Cuts at: 5 52 3125 4108 5
Size: 47 3073 983 2081
Fragments arranged by size:
3073 2081 983 47
StuI AGG'CCT
Cuts at: 1517 3438 1517
Size: 1921 4263
StyI C'CwwG_G
Cuts at: 513 1065 3623 513
Size: 552 2558 3074
Fragments arranged by size:
3074 2558 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2378 4211 4228 4381 4566 5011 325
Size: 1255 798 1833 17 153 185 445 1498
Fragments arranged by size:
1833 1498 1255 798 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4430 6142 96
Size: 274 80 33 51 1443 2453 1712 138
Fragments arranged by size:
2453 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2353 2385 6144 6176 2353
Size: 32 3759 32 2361
Fragments arranged by size:
3759 2361 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6184
XbaI T'CTAG_A
Cuts at: 3520 3520
Size: 6184
XhoI C'TCGA_G
Cuts at: 1943 2057 1943
Size: 114 6070
XmaI C'CCGG_G
Cuts at: 1870 3509 1870
Size: 1639 4545
XmnI GAAnn'nnTTC
Cuts at: 4313 4313
Size: 6184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3992 276
Size: 53 83 186 936 2458 2468
Fragments arranged by size:
2468 2458 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI