Restriction map for
pFN21AE3215
(Circular) (Six-base) MAPSORT of: pFN21AE3215.seq Check: 9898 from: 1 to: 6178
pFN21AE3215 6178 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2956 2956
Size: 6178
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3521 1937
Size: 1584 4594
Acc65I G'GTAC_C
Cuts at: 3499 3499
Size: 6178
AclI AA'CG_TT
Cuts at: 1563 4305 4678 1563
Size: 2742 373 3063
Fragments arranged by size:
3063 2742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3324 4241 5625 5893 1632
Size: 1692 917 1384 268 1917
Fragments arranged by size:
1917 1692 1384 917 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 2743 5077 6054 1203
Size: 1540 2334 977 1327
Fragments arranged by size:
2334 1540 1327 977
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 4907 4907
Size: 6178
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3042 1184
Size: 1858 4320
AlwNI CAG_nnn'CTG
Cuts at: 2948 3921 5493 2948
Size: 973 1572 3633
Fragments arranged by size:
3633 1572 973
ApaLI G'TGCA_C
Cuts at: 4235 5391 4235
Size: 1156 5022
ApoI r'AATT_y
Cuts at: 1493 2079 2908 3487 3713 3865 5974 1493
Size: 586 829 579 226 152 2109 1697
Fragments arranged by size:
2109 1697 829 586 579 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4732 160
Size: 872 3700 1606
Fragments arranged by size:
3700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 3503 1870
Size: 73 1560 4545
Fragments arranged by size:
4545 1560 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2304 2337 2304
Size: 33 6145
BamHI G'GATC_C
Cuts at: 2048 3508 3875 2048
Size: 1460 367 4351
Fragments arranged by size:
4351 1460 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3499 4954 618
Size: 325 217 627 1712 1455 1842
Fragments arranged by size:
1842 1712 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3326 3497 1391
Size: 1935 171 4072
Fragments arranged by size:
4072 1935 171
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5207
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4369 4403 1903
Size: 34 2432 34 3678
Fragments arranged by size:
3678 2432 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4070 5280 4070
Size: 1210 4968
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4789 136
Size: 107 122 71 631 605 3117 1525
Fragments arranged by size:
3117 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6173 6173
Size: 6178
BlpI GC'TnA_GC
Cuts at: 3595 3595
Size: 6178
Bme1580I G_kGCm'C
Cuts at: 1790 4239 5395 1790
Size: 2449 1156 2573
Fragments arranged by size:
2573 2449 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3289 3909 4867
Size: 416 53 83 399 24 1872 620 958
Cuts at: 4867 442
Size: 1753
Fragments arranged by size:
1872 1753 958 620 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2507 2507
Size: 6178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2778 3198 4838 1239
Size: 1539 420 1640 2579
Fragments arranged by size:
2579 1640 1539 420
Bpu10I CC'TnA_GC
Cuts at: 2655 2655
Size: 6178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4303 5168 5466 4303
Size: 865 298 5015
Fragments arranged by size:
5015 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4841 882
Size: 617 3342 2219
Fragments arranged by size:
3342 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3969 493
Size: 711 2765 2702
Fragments arranged by size:
2765 2702 711
BsaBI GATnn'nnATC
Cuts at: 2291 6172 2291
Size: 3881 2297
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3985 4367 5002 275
Size: 53 83 186 936 2452 382 635 1451
Fragments arranged by size:
2452 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4611 5009 5283 5430 5841 4611
Size: 398 274 147 411 4948
Fragments arranged by size:
4948 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3127 3157 1178
Size: 30 165 30 1724 30 4199
Fragments arranged by size:
4199 1724 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2781 3033 3087 3201 3255 1186
Size: 1595 252 54 114 54 4109
Fragments arranged by size:
4109 1595 252 114 54 54
BseYI C'CCAG_C
Cuts at: 2941 5381 5877 2941
Size: 2440 496 3242
Fragments arranged by size:
3242 2440 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2109 2480 3240 3354 1163
Size: 573 373 371 760 114 3987
Fragments arranged by size:
3987 760 573 373 371 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2831 3628 4389 5417 1540
Size: 275 186 830 797 761 1028 2301
Fragments arranged by size:
2301 1028 830 797 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2760 2791 4239 4324 5395 2760
Size: 31 1448 85 1071 3543
Fragments arranged by size:
3543 1448 1071 85 31
BsmI GAATG_Cn'
Cuts at: 3689 3782 3689
Size: 93 6085
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2760 2791 3326 3497 4239 4324 5395
Size: 399 970 31 535 171 742 85 1071
Cuts at: 5395 1391
Size: 2174
Fragments arranged by size:
2174 1071 970 742 535 399 171 85
31
BspEI T'CCGG_A
Cuts at: 5841 5841
Size: 6178
BspHI T'CATG_A
Cuts at: 2718 4067 5797 2718
Size: 1349 1730 3099
Fragments arranged by size:
3099 1730 1349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2131 2956 3080 3533 844
Size: 1287 825 124 453 3489
Fragments arranged by size:
3489 1287 825 453 124
BsrBI CCG'CTC
Cuts at: 1394 1823 4065 1394
Size: 429 2242 3507
Fragments arranged by size:
3507 2242 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4673 4847 65
Size: 1183 3425 174 1396
Fragments arranged by size:
3425 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4822 5009 1700
Size: 110 3012 187 2869
Fragments arranged by size:
3012 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2477 96
Size: 2381 3797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6178
BssSI C'ACGA_G
Cuts at: 2481 4238 5250 2481
Size: 1757 1012 3409
Fragments arranged by size:
3409 1757 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2746 794
Size: 1952 4226
BstBI TT'CG_AA
Cuts at: 4982 4982
Size: 6178
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6178
BstYI r'GATC_y
Cuts at: 1972 2048 3508 3875 4260 4277 5729 5815 5827
Size: 76 1460 367 385 17 1452 86 12
Cuts at: 5827 6173 1972
Size: 346 1977
Fragments arranged by size:
1977 1460 1452 385 367 346 86 76
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2808 513
Size: 552 939 804 3883
Fragments arranged by size:
3883 939 804 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2552 487
Size: 683 56 1326 4113
Fragments arranged by size:
4113 1326 683 56
BtsI GCAGTG_nn'
Cuts at: 2480 3694 4488 4508 2480
Size: 1214 794 20 4150
Fragments arranged by size:
4150 1214 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2718 2753 529
Size: 35 2154 35 3954
Fragments arranged by size:
3954 2154 35 35
DraI TTT'AAA
Cuts at: 3482 3482
Size: 6178
DraIII CAC_nnn'GTG
Cuts at: 2177 2177
Size: 6178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5185 809
Size: 507 3869 1802
Fragments arranged by size:
3869 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3625 4514 6143
Size: 54 1425 50 275 18 1795 889 1629
Cuts at: 6143 8
Size: 43
Fragments arranged by size:
1795 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3625 1537
Size: 275 1813 4090
Fragments arranged by size:
4090 1813 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2498 4108 1353
Size: 1145 1610 3423
Fragments arranged by size:
3423 1610 1145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2691 3399 4765 5139 5285 387
Size: 1064 138 1102 708 1366 374 146 1280
Fragments arranged by size:
1366 1280 1102 1064 708 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2778 3198 3324 4241 4838 5625 5893
Size: 393 1146 420 126 917 597 787 268
Cuts at: 5893 1239
Size: 1524
Fragments arranged by size:
1524 1146 917 787 597 420 393 268
126
EcoNI CCTnn'n_nnAGG
Cuts at: 3213 3213
Size: 6178
EcoO109I rG'GnC_Cy
Cuts at: 3218 3218
Size: 6178
EcoRI G'AATT_C
Cuts at: 2079 3487 3865 2079
Size: 1408 378 4392
Fragments arranged by size:
4392 1408 378
FspI TGC'GCA
Cuts at: 4684 4684
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 5325 1678
Size: 3647 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3127 3159 4819 4851 4893
Size: 32 135 32 1555 32 1660 32 42
Cuts at: 4893 4925 1373
Size: 32 2626
Fragments arranged by size:
2626 1660 1555 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2978 3522 3768 669
Size: 988 281 1040 544 246 3079
Fragments arranged by size:
3079 1040 988 544 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6178
HpaI GTT'AAC
Cuts at: 3768 3768
Size: 6178
KpnI G_GTAC'C
Cuts at: 3503 3503
Size: 6178
MfeI C'AATT_G
Cuts at: 3777 3777
Size: 6178
MluI A'CGCG_T
Cuts at: 6054 6054
Size: 6178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5292 5476 1305
Size: 3987 184 2007
Fragments arranged by size:
3987 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4356
Fragments arranged by size:
4356 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2328 2720 4136 4495 4654 518
Size: 648 1162 392 1416 359 159 2042
Fragments arranged by size:
2042 1416 1162 648 392 359 159
MspA1I CmG'CkG
Cuts at: 1606 3594 4271 5419 5664 1606
Size: 1988 677 1148 245 2120
Fragments arranged by size:
2120 1988 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2808 513
Size: 552 939 804 3883
Fragments arranged by size:
3883 939 804 552
NdeI CA'TA_TG
Cuts at: 387 2574 2688 387
Size: 2187 114 3877
Fragments arranged by size:
3877 2187 114
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 2387 2747 3536 5081 1132
Size: 97 1158 360 789 1545 2229
Fragments arranged by size:
2229 1545 1158 789 360 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6178
PciI A'CATG_T
Cuts at: 2743 5077 2743
Size: 2334 3844
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3431 1108
Size: 2323 3855
PfoI T'CCnGG_A
Cuts at: 2260 3214 3430 2260
Size: 954 216 5008
Fragments arranged by size:
5008 954 216
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 5760 5792 4247
Size: 32 1481 32 4633
Fragments arranged by size:
4633 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3218 3218
Size: 6178
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6178
PsiI TTA'TAA
Cuts at: 2199 2666 3276 3748 2199
Size: 467 610 472 4629
Fragments arranged by size:
4629 610 472 467
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2470 2502 2470
Size: 32 6146
PstI C_TGCA'G
Cuts at: 830 3091 3530 830
Size: 2261 439 3478
Fragments arranged by size:
3478 2261 439
PvuI CG_AT'CG
Cuts at: 2001 2831 2001
Size: 830 5348
SalI G'TCGA_C
Cuts at: 1936 3520 1936
Size: 1584 4594
SbfI CC_TGCA'GG
Cuts at: 3530 3530
Size: 6178
ScaI AGT'ACT
Cuts at: 4426 6138 4426
Size: 1712 4466
SexAI A'CCwGG_T
Cuts at: 2173 2173
Size: 6178
SfcI C'TryA_G
Cuts at: 826 1045 2446 3087 3526 4661 5342 5533 826
Size: 219 1401 641 439 1135 681 191 1471
Fragments arranged by size:
1471 1401 1135 681 641 439 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 3505 1872
Size: 1633 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4282 5183 5445 820
Size: 197 926 2339 901 262 1553
Fragments arranged by size:
2339 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 3536 1132
Size: 2404 3774
SspI AAT'ATT
Cuts at: 5 52 4102 5
Size: 47 4050 2081
Fragments arranged by size:
4050 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6178
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 2808 3617 513
Size: 552 939 48 756 809 3074
Fragments arranged by size:
3074 939 809 756 552 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 2821 4205 4222 4375 4560 5005
Size: 1255 465 776 1384 17 153 185 445
Cuts at: 5005 325
Size: 1498
Fragments arranged by size:
1498 1384 1255 776 465 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2477 4424 6136
Size: 274 80 33 51 1443 500 1947 1712
Cuts at: 6136 96
Size: 138
Fragments arranged by size:
1947 1712 1443 500 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6138 6170 6138
Size: 32 6146
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6178
XbaI T'CTAG_A
Cuts at: 3514 3514
Size: 6178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2579 2579
Size: 6178
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6178
XmaI C'CCGG_G
Cuts at: 1870 3503 1870
Size: 1633 4545
XmnI GAAnn'nnTTC
Cuts at: 2616 2847 4307 2616
Size: 231 1460 4487
Fragments arranged by size:
4487 1460 231
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI