Restriction map for
pFN21AE3209
(Circular) (Six-base) MAPSORT of: pFN21AE3209.seq Check: 813 from: 1 to: 6139
pFN21AE3209 6139 bp
With 182 enzymes: SgfI *
June 13, 2018 20:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2526 3052 2526
Size: 526 5613
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3949 278
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2331 3482 1937
Size: 394 1151 4594
Fragments arranged by size:
4594 1151 394
Acc65I G'GTAC_C
Cuts at: 3141 3460 3141
Size: 319 5820
AclI AA'CG_TT
Cuts at: 1563 4266 4639 1563
Size: 2703 373 3063
Fragments arranged by size:
3063 2703 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2293 2460 3167 3416 4202 5586 5854 1632
Size: 661 167 707 249 786 1384 268 1917
Fragments arranged by size:
1917 1384 786 707 661 268 249 167
AfeI AGC'GCT
Cuts at: 2439 2439
Size: 6139
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5942
AflIII A'CryG_T
Cuts at: 1203 3411 5038 6015 1203
Size: 2208 1627 977 1327
Fragments arranged by size:
2208 1627 1327 977
AgeI A'CCGG_T
Cuts at: 4970 4970
Size: 6139
AhdI GACnn_n'nnGTC
Cuts at: 4868 4868
Size: 6139
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6139
AlwNI CAG_nnn'CTG
Cuts at: 2053 2518 3067 3882 5454 2053
Size: 465 549 815 1572 2738
Fragments arranged by size:
2738 1572 815 549 465
ApaI G_GGCC'C
Cuts at: 2046 2116 2210 2501 2895 2046
Size: 70 94 291 394 5290
Fragments arranged by size:
5290 394 291 94 70
ApaLI G'TGCA_C
Cuts at: 4196 5352 4196
Size: 1156 4983
ApoI r'AATT_y
Cuts at: 1493 3448 3674 3826 5935 1493
Size: 1955 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4693 160
Size: 872 3661 1606
Fragments arranged by size:
3661 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2039 2433 2441 2698 2825 3464
Size: 73 89 7 394 8 257 127 639
Cuts at: 3464 1870
Size: 4545
Fragments arranged by size:
4545 639 394 257 127 89 73 8
7
BamHI G'GATC_C
Cuts at: 3469 3836 3469
Size: 367 5772
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2178 2584 2704 3141 3460
Size: 325 217 627 391 406 120 437 319
Cuts at: 3460 4915 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 437 406 391 325 319
217 120
BanII G_rGCy'C
Cuts at: 1391 2046 2116 2210 2501 2772 2832 2895 3327
Size: 655 70 94 291 271 60 63 432
Cuts at: 3327 3458 1391
Size: 131 4072
Fragments arranged by size:
4072 655 432 291 271 131 94 70
63 60
BbeI G_GCGC'C
Cuts at: 2588 2588
Size: 6139
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3089 928
Size: 971 1190 3978
Fragments arranged by size:
3978 1190 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4330 4364 1903
Size: 34 2393 34 3678
Fragments arranged by size:
3678 2393 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4031 5241 4031
Size: 1210 4929
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6139
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2271 2428 2652
Size: 107 122 71 631 605 599 157 224
Cuts at: 2652 2882 4750 136
Size: 230 1868 1525
Fragments arranged by size:
1868 1525 631 605 599 230 224 157
122 107 71
BglII A'GATC_T
Cuts at: 6134 6134
Size: 6139
BlpI GC'TnA_GC
Cuts at: 3556 3556
Size: 6139
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2116 2210 2501 2709 2895 4200 5356
Size: 256 70 94 291 208 186 1305 1156
Cuts at: 5356 1790
Size: 2573
Fragments arranged by size:
2573 1305 1156 291 256 208 186 94
70
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6139
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2898 3870 4828
Size: 416 53 83 399 24 1481 972 958
Cuts at: 4828 442
Size: 1753
Fragments arranged by size:
1753 1481 972 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2882 4799 1239
Size: 917 726 1917 2579
Fragments arranged by size:
2579 1917 917 726
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4264 5129 5427 4264
Size: 865 298 4976
Fragments arranged by size:
4976 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2814 4802 882
Size: 617 1315 1988 2219
Fragments arranged by size:
2219 1988 1315 617
BsaAI yAC'GTr
Cuts at: 493 1204 3930 493
Size: 711 2726 2702
Fragments arranged by size:
2726 2702 711
BsaBI GATnn'nnATC
Cuts at: 3378 6133 3378
Size: 2755 3384
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2585 3946 4328 4963
Size: 53 83 186 936 1052 1361 382 635
Cuts at: 4963 275
Size: 1451
Fragments arranged by size:
1451 1361 1052 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4572 4970 5244 5391 5802 4572
Size: 398 274 147 411 4909
Fragments arranged by size:
4909 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5914
Fragments arranged by size:
5914 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 2702 1186
Size: 1468 48 4623
Fragments arranged by size:
4623 1468 48
BseYI C'CCAG_C
Cuts at: 2046 2210 2673 2714 2978 5342 5838 2046
Size: 164 463 41 264 2364 496 2347
Fragments arranged by size:
2364 2347 496 463 264 164 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5566
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2142 2196 2282 3589 4350 5378
Size: 275 186 141 54 86 1307 761 1028
Cuts at: 5378 1540
Size: 2301
Fragments arranged by size:
2301 1307 1028 761 275 186 141 86
54
BsiHKAI G_wGCw'C
Cuts at: 3134 4200 4285 5356 3134
Size: 1066 85 1071 3917
Fragments arranged by size:
3917 1071 1066 85
BsmI GAATG_Cn'
Cuts at: 3650 3743 3650
Size: 93 6046
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2116 2210 2501 2709 2772 2832
Size: 399 256 70 94 291 208 63 60
Cuts at: 2832 2895 3134 3327 3458 4200 4285 5356 1391
Size: 63 239 193 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 291 256 239 208
193 131 94 85 70 63 63 60
BspEI T'CCGG_A
Cuts at: 5802 5802
Size: 6139
BspHI T'CATG_A
Cuts at: 4028 5758 4028
Size: 1730 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2526 3052 3494 844
Size: 1682 526 442 3489
Fragments arranged by size:
3489 1682 526 442
BsrBI CCG'CTC
Cuts at: 1394 1823 2021 4026 1394
Size: 429 198 2005 3507
Fragments arranged by size:
3507 2005 429 198
BsrDI GCAATG_nn'
Cuts at: 65 1248 4634 4808 65
Size: 1183 3386 174 1396
Fragments arranged by size:
3386 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2099 2265 4783 4970 1700
Size: 110 289 166 2518 187 2869
Fragments arranged by size:
2869 2518 289 187 166 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6139
BssHII G'CGCG_C
Cuts at: 1925 2274 2938 1925
Size: 349 664 5126
Fragments arranged by size:
5126 664 349
BssSI C'ACGA_G
Cuts at: 4199 5211 4199
Size: 1012 5127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6139
BstBI TT'CG_AA
Cuts at: 4943 4943
Size: 6139
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6139
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2755 2779 2581
Size: 174 24 5941
Fragments arranged by size:
5941 174 24
BstYI r'GATC_y
Cuts at: 1972 2167 3469 3836 4221 4238 5690 5776 5788
Size: 195 1302 367 385 17 1452 86 12
Cuts at: 5788 6134 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1302 385 367 346 195 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 2459 2566 2574 3417 513
Size: 552 939 132 323 107 8 843 3235
Fragments arranged by size:
3235 939 843 552 323 132 107 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5400
Fragments arranged by size:
5400 683 56
BtsI GCAGTG_nn'
Cuts at: 2129 2790 3116 3655 4449 4469 2129
Size: 661 326 539 794 20 3799
Fragments arranged by size:
3799 794 661 539 326 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6104
DraI TTT'AAA
Cuts at: 3443 3443
Size: 6139
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5146 809
Size: 507 3830 1802
Fragments arranged by size:
3830 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2139 2193
Size: 54 1425 50 275 18 267 42 54
Cuts at: 2193 2279 2394 2547 2757 3005 3051 3586 4475
Size: 86 115 153 210 248 46 535 889
Cuts at: 4475 6104 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 535 275 267 248 210
153 115 86 54 54 50 46 43
42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2139 2193 2279 3586 1537
Size: 275 327 54 86 1307 4090
Fragments arranged by size:
4090 1307 327 275 86 54
EarI CTCTTCn'nnn_
Cuts at: 1353 2528 3371 4069 1353
Size: 1175 843 698 3423
Fragments arranged by size:
3423 1175 843 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2659 4726 5100 5246 387
Size: 1064 138 1070 2067 374 146 1280
Fragments arranged by size:
2067 1280 1070 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2293 2460 2882 3167 3416 4202
Size: 393 524 137 167 422 285 249 786
Cuts at: 4202 4799 5586 5854 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 786 597 524 422 393 285
268 249 167 137
EcoNI CCTnn'n_nnAGG
Cuts at: 2606 2606
Size: 6139
EcoO109I rG'GnC_Cy
Cuts at: 2036 2043 2112 2113 2207 2695 2892 3361 2036
Size: 7 69 1 94 488 197 469 4814
Fragments arranged by size:
4814 488 469 197 94 69 7 1
EcoRI G'AATT_C
Cuts at: 3448 3826 3448
Size: 378 5761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3172 3172
Size: 6139
FspI TGC'GCA
Cuts at: 4645 4645
Size: 6139
HaeII r_GCGC'y
Cuts at: 1678 2217 2441 2588 2996 5286 1678
Size: 539 224 147 408 2290 2531
Fragments arranged by size:
2531 2290 539 408 224 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3241 3273 4780 4812 4854
Size: 32 135 32 1669 32 1507 32 42
Cuts at: 4854 4886 1373
Size: 32 2626
Fragments arranged by size:
2626 1669 1507 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2332 3483 3729 669
Size: 988 281 394 1151 246 3079
Fragments arranged by size:
3079 1151 988 394 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6139
HpaI GTT'AAC
Cuts at: 3729 3729
Size: 6139
KasI G'GCGC_C
Cuts at: 2584 2584
Size: 6139
KpnI G_GTAC'C
Cuts at: 3145 3464 3145
Size: 319 5820
MfeI C'AATT_G
Cuts at: 3738 3738
Size: 6139
MluI A'CGCG_T
Cuts at: 6015 6015
Size: 6139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3288 5253 5437 1305
Size: 1983 1965 184 2007
Fragments arranged by size:
2007 1983 1965 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2759 10
Size: 54 1425 343 927 3390
Fragments arranged by size:
3390 1425 927 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3416 4097 4456 4615 518
Size: 648 2250 681 359 159 2042
Fragments arranged by size:
2250 2042 681 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2138 2176 2461 2488 2568 2677 2855
Size: 444 88 38 285 27 80 109 178
Cuts at: 2855 2971 3555 4232 5380 5625 1606
Size: 116 584 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 584 444 285 245 178
116 109 88 80 38 27
NaeI GCC'GGC
Cuts at: 2101 2267 2101
Size: 166 5973
NarI GG'CG_CC
Cuts at: 2585 2585
Size: 6139
NcoI C'CATG_G
Cuts at: 513 1065 2004 2574 513
Size: 552 939 570 4078
Fragments arranged by size:
4078 939 570 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6139
NgoMIV G'CCGG_C
Cuts at: 2099 2265 2099
Size: 166 5973
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6139
NotI GC'GGCC_GC
Cuts at: 2139 2193 3586 2139
Size: 54 1393 4692
Fragments arranged by size:
4692 1393 54
NspI r_CATG'y
Cuts at: 1132 1229 2242 3231 3415 3497 5042 1132
Size: 97 1013 989 184 82 1545 2229
Fragments arranged by size:
2229 1545 1013 989 184 97 82
PasI CC'CwG_GG
Cuts at: 1348 2183 2494 1348
Size: 835 311 4993
Fragments arranged by size:
4993 835 311
PciI A'CATG_T
Cuts at: 3411 5038 3411
Size: 1627 4512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2224 1108
Size: 1116 5023
PfoI T'CCnGG_A
Cuts at: 2088 2898 3134 3369 2088
Size: 810 236 235 4858
Fragments arranged by size:
4858 810 236 235
PmeI GTTT'AAAC
Cuts at: 3443 3443
Size: 6139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 5721 5753 4208
Size: 32 1481 32 4594
Fragments arranged by size:
4594 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2036 2695 3361 2036
Size: 659 666 4814
Fragments arranged by size:
4814 666 659
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6139
PsiI TTA'TAA
Cuts at: 3709 3709
Size: 6139
PspOMI G'GGCC_C
Cuts at: 2042 2112 2206 2497 2891 2042
Size: 70 94 291 394 5290
Fragments arranged by size:
5290 394 291 94 70
PspXI vC'TCGA_Gb
Cuts at: 2433 2441 2433
Size: 8 6131
PstI C_TGCA'G
Cuts at: 830 2157 2796 3491 830
Size: 1327 639 695 3478
Fragments arranged by size:
3478 1327 695 639
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6139
PvuII CAG'CTG
Cuts at: 2050 2677 2971 2050
Size: 627 294 5218
Fragments arranged by size:
5218 627 294
RsrII CG'GwC_CG
Cuts at: 2299 2299
Size: 6139
SacII CC_GC'GG
Cuts at: 2139 2462 2569 2139
Size: 323 107 5709
Fragments arranged by size:
5709 323 107
SalI G'TCGA_C
Cuts at: 1936 2330 3481 1936
Size: 394 1151 4594
Fragments arranged by size:
4594 1151 394
SanDI GG'GwC_CC
Cuts at: 2036 2695 2036
Size: 659 5480
SapI GCTCTTCn'nnn_
Cuts at: 2528 2528
Size: 6139
SbfI CC_TGCA'GG
Cuts at: 2157 3491 2157
Size: 1334 4805
ScaI AGT'ACT
Cuts at: 4387 6099 4387
Size: 1712 4427
SfcI C'TryA_G
Cuts at: 826 1045 2153 2792 3487 4622 5303 5494 826
Size: 219 1108 639 695 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 695 681 639 219 191
SfoI GGC'GCC
Cuts at: 2586 2586
Size: 6139
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6139
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6139
SmaI CCC'GGG
Cuts at: 1872 2034 2041 2700 3466 1872
Size: 162 7 659 766 4545
Fragments arranged by size:
4545 766 659 162 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2433 2441 4243 5144 5406 820
Size: 197 926 490 8 1802 901 262 1553
Fragments arranged by size:
1802 1553 926 901 490 262 197 8
SnaBI TAC'GTA
Cuts at: 493 3930 493
Size: 3437 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6139
SphI G_CATG'C
Cuts at: 1132 3497 1132
Size: 2365 3774
SspI AAT'ATT
Cuts at: 5 52 4063 5
Size: 47 4011 2081
Fragments arranged by size:
4011 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6139
StyI C'CwwG_G
Cuts at: 513 1065 2004 2574 2613 3355 3578 513
Size: 552 939 570 39 742 223 3074
Fragments arranged by size:
3074 939 742 570 552 223 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4166 4183 4336 4521 4966 325
Size: 1255 2586 17 153 185 445 1498
Fragments arranged by size:
2586 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 4385 6097
Size: 274 80 33 51 1443 282 2126 1712
Cuts at: 6097 96
Size: 138
Fragments arranged by size:
2126 1712 1443 282 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2940 2972 6099 6131 2940
Size: 32 3127 32 2948
Fragments arranged by size:
3127 2948 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2225 1316
Size: 909 5230
XbaI T'CTAG_A
Cuts at: 3475 3475
Size: 6139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2180 2323 3415 2180
Size: 143 1092 4904
Fragments arranged by size:
4904 1092 143
XhoI C'TCGA_G
Cuts at: 1943 2433 2441 1943
Size: 490 8 5641
Fragments arranged by size:
5641 490 8
XmaI C'CCGG_G
Cuts at: 1870 2032 2039 2698 3464 1870
Size: 162 7 659 766 4545
Fragments arranged by size:
4545 766 659 162 7
XmnI GAAnn'nnTTC
Cuts at: 4268 4268
Size: 6139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3947 276
Size: 53 83 186 936 2413 2468
Fragments arranged by size:
2468 2413 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI NruI NsiI PacI PmlI PsrI SacI SexAI
SfiI SrfI SwaI