Restriction map for
pFN21AE3207
(Circular) (Six-base) MAPSORT of: pFN21AE3207.seq Check: 8804 from: 1 to: 6136
pFN21AE3207 6136 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3946 278
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3479 1937
Size: 1542 4594
Acc65I G'GTAC_C
Cuts at: 3457 3457
Size: 6136
AclI AA'CG_TT
Cuts at: 1563 3435 4263 4636 1563
Size: 1872 828 373 3063
Fragments arranged by size:
3063 1872 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4199 5583 5851 1632
Size: 2567 1384 268 1917
Fragments arranged by size:
2567 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3053 820
Size: 197 2036 3903
Fragments arranged by size:
3903 2036 197
AflIII A'CryG_T
Cuts at: 1203 5035 6012 1203
Size: 3832 977 1327
Fragments arranged by size:
3832 1327 977
AgeI A'CCGG_T
Cuts at: 4967 4967
Size: 6136
AhdI GACnn_n'nnGTC
Cuts at: 2244 4865 2244
Size: 2621 3515
AleI CACnn'nnGTG
Cuts at: 1166 2886 1166
Size: 1720 4416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2218 1184
Size: 1034 5102
AlwNI CAG_nnn'CTG
Cuts at: 2605 2774 3879 5451 2605
Size: 169 1105 1572 3290
Fragments arranged by size:
3290 1572 1105 169
ApaLI G'TGCA_C
Cuts at: 4193 5349 4193
Size: 1156 4980
ApoI r'AATT_y
Cuts at: 1493 2504 3120 3213 3445 3671 3823 5932 1493
Size: 1011 616 93 232 226 152 2109 1697
Fragments arranged by size:
2109 1697 1011 616 232 226 152 93
AseI AT'TA_AT
Cuts at: 160 1032 2709 4690 160
Size: 872 1677 1981 1606
Fragments arranged by size:
1981 1677 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
AvaI C'yCGr_G
Cuts at: 1870 1943 2301 2309 2317 3461 1870
Size: 73 358 8 8 1144 4545
Fragments arranged by size:
4545 1144 358 73 8 8
BamHI G'GATC_C
Cuts at: 2357 2365 3466 3833 2357
Size: 8 1101 367 4660
Fragments arranged by size:
4660 1101 367 8
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 3457 4912 618
Size: 325 217 627 232 1438 1455 1842
Fragments arranged by size:
1842 1455 1438 627 325 232 217
BanII G_rGCy'C
Cuts at: 1391 2058 2097 2110 2310 3455 1391
Size: 667 39 13 200 1145 4072
Fragments arranged by size:
4072 1145 667 200 39 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2661 2976 928
Size: 971 762 315 4088
Fragments arranged by size:
4088 971 762 315
BbvCI CC'TCA_GC
Cuts at: 2636 2636
Size: 6136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4327 4361 1903
Size: 34 50 34 2306 34 3678
Fragments arranged by size:
3678 2306 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4028 5238 4028
Size: 1210 4926
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6136
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4747 136
Size: 107 122 71 631 605 3075 1525
Fragments arranged by size:
3075 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6131 6131
Size: 6136
BlpI GC'TnA_GC
Cuts at: 3553 3553
Size: 6136
Bme1580I G_kGCm'C
Cuts at: 1790 4197 5353 1790
Size: 2407 1156 2573
Fragments arranged by size:
2573 2407 1156
BmgBI CAC'GTC
Cuts at: 1318 2419 1318
Size: 1101 5035
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2383 2496 2563
Size: 416 53 83 399 24 966 113 67
Cuts at: 2563 3867 4825 442
Size: 1304 958 1753
Fragments arranged by size:
1753 1304 966 958 416 399 113 83
67 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4796 1239
Size: 3557 2579
Bpu10I CC'TnA_GC
Cuts at: 2636 2767 2636
Size: 131 6005
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3252 4261 5126 5424 3252
Size: 1009 865 298 3964
Fragments arranged by size:
3964 1009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2133 2319 2443 4799 882
Size: 617 634 186 124 2356 2219
Fragments arranged by size:
2356 2219 634 617 186 124
BsaAI yAC'GTr
Cuts at: 493 1204 3927 493
Size: 711 2723 2702
Fragments arranged by size:
2723 2702 711
BsaBI GATnn'nnATC
Cuts at: 2732 6130 2732
Size: 3398 2738
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3943 4325 4960 275
Size: 53 83 186 936 2410 382 635 1451
Fragments arranged by size:
2410 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4569 4967 5241 5388 5799 4569
Size: 398 274 147 411 4906
Fragments arranged by size:
4906 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5911
Fragments arranged by size:
5911 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2623 2653 1186
Size: 1437 30 4669
Fragments arranged by size:
4669 1437 30
BseYI C'CCAG_C
Cuts at: 2462 5339 5835 2462
Size: 2877 496 2763
Fragments arranged by size:
2877 2763 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 1163
Size: 573 324 5239
Fragments arranged by size:
5239 573 324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3586 4347 5375 1540
Size: 275 186 1585 761 1028 2301
Fragments arranged by size:
2301 1585 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2110 2166 2310 4197 4282 5353 2110
Size: 56 144 1887 85 1071 2893
Fragments arranged by size:
2893 1887 1071 144 85 56
BsmI GAATG_Cn'
Cuts at: 3325 3647 3740 3325
Size: 322 93 5721
Fragments arranged by size:
5721 322 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2425 2425
Size: 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2097 2110 2166 2310 3455 4197
Size: 399 268 39 13 56 144 1145 742
Cuts at: 4197 4282 5353 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1145 1071 742 399 268 144 85
56 39 13
BspEI T'CCGG_A
Cuts at: 5799 5799
Size: 6136
BspHI T'CATG_A
Cuts at: 4025 5755 4025
Size: 1730 4406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 2151 3491 844
Size: 1271 36 1340 3489
Fragments arranged by size:
3489 1340 1271 36
BsrBI CCG'CTC
Cuts at: 1394 1823 2322 4023 1394
Size: 429 499 1701 3507
Fragments arranged by size:
3507 1701 499 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4631 4805 65
Size: 1183 3383 174 1396
Fragments arranged by size:
3383 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2334 4780 4967 1700
Size: 110 524 2446 187 2869
Fragments arranged by size:
2869 2446 524 187 110
BsrGI T'GTAC_A
Cuts at: 96 3253 96
Size: 3157 2979
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6136
BssSI C'ACGA_G
Cuts at: 2165 4196 5208 2165
Size: 2031 1012 3093
Fragments arranged by size:
3093 2031 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2632 794
Size: 1838 4298
BstBI TT'CG_AA
Cuts at: 4940 4940
Size: 6136
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6136
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3375 2011
Size: 1364 4772
BstYI r'GATC_y
Cuts at: 1972 2357 2365 3466 3833 4218 4235 5687 5773
Size: 385 8 1101 367 385 17 1452 86
Cuts at: 5773 5785 6131 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1101 385 385 367 346 86
17 12 8
BtgI C'CryG_G
Cuts at: 513 1065 2023 513
Size: 552 958 4626
Fragments arranged by size:
4626 958 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5397
Fragments arranged by size:
5397 683 56
BtsI GCAGTG_nn'
Cuts at: 3652 4446 4466 3652
Size: 794 20 5322
Fragments arranged by size:
5322 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6101
DraI TTT'AAA
Cuts at: 3440 3440
Size: 6136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5143 809
Size: 507 3827 1802
Fragments arranged by size:
3827 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2596 2921 3583
Size: 54 1425 50 275 18 766 325 662
Cuts at: 3583 4472 6101 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 766 662 325 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3583 1537
Size: 275 1771 4090
Fragments arranged by size:
4090 1771 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 2403 2910 4066 1353
Size: 930 120 507 1156 3423
Fragments arranged by size:
3423 1156 930 507 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4723 5097 5243 387
Size: 1064 138 3134 374 146 1280
Fragments arranged by size:
3134 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2108 2308 2108
Size: 200 5936
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4199 4796 5583 5851 1239
Size: 393 2567 597 787 268 1524
Fragments arranged by size:
2567 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2087 2048
Size: 39 6097
EcoO109I rG'GnC_Cy
Cuts at: 2127 2127
Size: 6136
EcoRI G'AATT_C
Cuts at: 3120 3445 3823 3120
Size: 325 378 5433
Fragments arranged by size:
5433 378 325
EcoRV GAT'ATC
Cuts at: 2734 2734
Size: 6136
FspI TGC'GCA
Cuts at: 4642 4642
Size: 6136
HaeII r_GCGC'y
Cuts at: 1678 5283 1678
Size: 3605 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2230 2262 2963 2995 4777
Size: 32 135 32 658 32 701 32 1782
Cuts at: 4777 4809 4851 4883 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1782 701 658 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3480 3726 669
Size: 988 281 1542 246 3079
Fragments arranged by size:
3079 1542 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6136
HpaI GTT'AAC
Cuts at: 3726 3726
Size: 6136
KpnI G_GTAC'C
Cuts at: 3461 3461
Size: 6136
MfeI C'AATT_G
Cuts at: 3735 3735
Size: 6136
MluI A'CGCG_T
Cuts at: 6012 6012
Size: 6136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2788 5250 5434 1305
Size: 1483 2462 184 2007
Fragments arranged by size:
2462 2007 1483 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2923 10
Size: 54 1425 343 1091 3223
Fragments arranged by size:
3223 1425 1091 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2886 3063 4094 4453 4612 518
Size: 648 843 877 177 1031 359 159 2042
Fragments arranged by size:
2042 1031 877 843 648 359 177 159
MspA1I CmG'CkG
Cuts at: 1606 2025 3552 4229 5377 5622 1606
Size: 419 1527 677 1148 245 2120
Fragments arranged by size:
2120 1527 1148 677 419 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5584
NdeI CA'TA_TG
Cuts at: 387 3340 387
Size: 2953 3183
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6136
NotI GC'GGCC_GC
Cuts at: 3583 3583
Size: 6136
NspI r_CATG'y
Cuts at: 1132 1229 2635 3494 5039 1132
Size: 97 1406 859 1545 2229
Fragments arranged by size:
2229 1545 1406 859 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6136
PciI A'CATG_T
Cuts at: 5035 5035
Size: 6136
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2860 1108
Size: 1752 4384
PmeI GTTT'AAAC
Cuts at: 3440 3440
Size: 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 5718 5750 4205
Size: 32 1481 32 4591
Fragments arranged by size:
4591 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2127 2127
Size: 6136
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6136
PsiI TTA'TAA
Cuts at: 3706 3706
Size: 6136
PspXI vC'TCGA_Gb
Cuts at: 2309 2317 2309
Size: 8 6128
PstI C_TGCA'G
Cuts at: 830 3488 830
Size: 2658 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6136
SacI G_AGCT'C
Cuts at: 2110 2310 2110
Size: 200 5936
SacII CC_GC'GG
Cuts at: 2026 2026
Size: 6136
SalI G'TCGA_C
Cuts at: 1936 3478 1936
Size: 1542 4594
SapI GCTCTTCn'nnn_
Cuts at: 2403 2403
Size: 6136
SbfI CC_TGCA'GG
Cuts at: 3488 3488
Size: 6136
ScaI AGT'ACT
Cuts at: 4384 6096 4384
Size: 1712 4424
SexAI A'CCwGG_T
Cuts at: 3382 3382
Size: 6136
SfcI C'TryA_G
Cuts at: 826 1045 3484 4619 5300 5491 826
Size: 219 2439 1135 681 191 1471
Fragments arranged by size:
2439 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6136
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6136
SmaI CCC'GGG
Cuts at: 1872 3463 1872
Size: 1591 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2301 2309 2317 3053 3267 4240
Size: 197 926 358 8 8 736 214 973
Cuts at: 4240 5141 5403 820
Size: 901 262 1553
Fragments arranged by size:
1553 973 926 901 736 358 262 214
197 8 8
SnaBI TAC'GTA
Cuts at: 493 3927 493
Size: 3434 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6136
SphI G_CATG'C
Cuts at: 1132 3494 1132
Size: 2362 3774
SspI AAT'ATT
Cuts at: 5 52 4060 5
Size: 47 4008 2081
Fragments arranged by size:
4008 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6136
StyI C'CwwG_G
Cuts at: 513 1065 2649 3575 513
Size: 552 1584 926 3074
Fragments arranged by size:
3074 1584 926 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2100 4163 4180 4333 4518 4963 325
Size: 1255 520 2063 17 153 185 445 1498
Fragments arranged by size:
2063 1498 1255 520 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2628 3253 3370
Size: 274 80 33 51 1443 651 625 117
Cuts at: 3370 4382 6094 96
Size: 1012 1712 138
Fragments arranged by size:
1712 1443 1012 651 625 274 138 117
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2342 2374 6096 6128 2342
Size: 32 3722 32 2350
Fragments arranged by size:
3722 2350 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6136
XbaI T'CTAG_A
Cuts at: 3472 3472
Size: 6136
XhoI C'TCGA_G
Cuts at: 1943 2301 2309 2317 1943
Size: 358 8 8 5762
Fragments arranged by size:
5762 358 8 8
XmaI C'CCGG_G
Cuts at: 1870 3461 1870
Size: 1591 4545
XmnI GAAnn'nnTTC
Cuts at: 2065 2531 4265 2065
Size: 466 1734 3936
Fragments arranged by size:
3936 1734 466
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3944 276
Size: 53 83 186 936 2410 2468
Fragments arranged by size:
2468 2410 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PsrI PvuII RsrII SanDI SfiI
SfoI SrfI SwaI XcmI