Restriction map for
pFN21AE3206
(Circular) (Six-base) MAPSORT of: pFN21AE3206.seq Check: 2277 from: 1 to: 6133
pFN21AE3206 6133 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3476 1937
Size: 1539 4594
Acc65I G'GTAC_C
Cuts at: 2231 3454 2231
Size: 1223 4910
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2181 2588 2999 3192 3348 4196 5580 5848
Size: 549 407 411 193 156 848 1384 268
Cuts at: 5848 1632
Size: 1917
Fragments arranged by size:
1917 1384 848 549 411 407 268 193
156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 2333 5032 6009 1203
Size: 1130 2699 977 1327
Fragments arranged by size:
2699 1327 1130 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AlwNI CAG_nnn'CTG
Cuts at: 2269 2585 3876 5448 2269
Size: 316 1291 1572 2954
Fragments arranged by size:
2954 1572 1291 316
ApaLI G'TGCA_C
Cuts at: 3030 4190 5346 3030
Size: 1160 1156 3817
Fragments arranged by size:
3817 1160 1156
ApoI r'AATT_y
Cuts at: 1493 2649 3213 3224 3442 3668 3820 5929 1493
Size: 1156 564 11 218 226 152 2109 1697
Fragments arranged by size:
2109 1697 1156 564 226 218 152 11
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 3458 1870
Size: 73 1515 4545
Fragments arranged by size:
4545 1515 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2223 2256 2223
Size: 33 6100
BamHI G'GATC_C
Cuts at: 3463 3830 3463
Size: 367 5766
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2231 3454 4909 618
Size: 325 217 627 444 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 444 325 217
BanII G_rGCy'C
Cuts at: 1391 3183 3452 1391
Size: 1792 269 4072
Fragments arranged by size:
4072 1792 269
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2594 928
Size: 971 695 4467
Fragments arranged by size:
4467 971 695
BbvCI CC'TCA_GC
Cuts at: 3164 3164
Size: 6133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4324 4358 1903
Size: 34 2387 34 3678
Fragments arranged by size:
3678 2387 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 2032 1548
Size: 484 5649
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4744 136
Size: 107 122 71 631 605 3072 1525
Fragments arranged by size:
3072 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3046 6128 3046
Size: 3082 3051
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 2718 3034 4194 5350 1790
Size: 928 316 1160 1156 2573
Fragments arranged by size:
2573 1160 1156 928 316
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3864 4822 442
Size: 416 53 83 399 24 2447 958 1753
Fragments arranged by size:
2447 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3437 4793 1239
Size: 2198 1356 2579
Fragments arranged by size:
2579 2198 1356
Bpu10I CC'TnA_GC
Cuts at: 3164 3182 3164
Size: 18 6115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4258 5123 5421 4258
Size: 865 298 4970
Fragments arranged by size:
4970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4796 882
Size: 617 3297 2219
Fragments arranged by size:
3297 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2123 2334 3924 493
Size: 711 919 211 1590 2702
Fragments arranged by size:
2702 1590 919 711 211
BsaBI GATnn'nnATC
Cuts at: 6127 6127
Size: 6133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3940 4322 4957 275
Size: 53 83 186 936 2407 382 635 1451
Fragments arranged by size:
2407 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3262 4566 4964 5238 5385 5796 3262
Size: 1304 398 274 147 411 3599
Fragments arranged by size:
3599 1304 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5908
Fragments arranged by size:
5908 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2467 3020 1186
Size: 1281 553 4299
Fragments arranged by size:
4299 1281 553
BseYI C'CCAG_C
Cuts at: 5336 5832 5336
Size: 496 5637
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5560
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2725 3034 3183 4194 4279 5350 2725
Size: 309 149 1011 85 1071 3508
Fragments arranged by size:
3508 1071 1011 309 149 85
BsmI GAATG_Cn'
Cuts at: 2383 3644 3737 2383
Size: 1261 93 4779
Fragments arranged by size:
4779 1261 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2718 2725 3034 3183 3452 4194 4279
Size: 399 928 7 309 149 269 742 85
Cuts at: 4279 5350 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 928 742 399 309 269 149
85 7
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 4022 5752 4022
Size: 1730 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2564 3488 844
Size: 1720 924 3489
Fragments arranged by size:
3489 1720 924
BsrBI CCG'CTC
Cuts at: 1394 1823 4020 1394
Size: 429 2197 3507
Fragments arranged by size:
3507 2197 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3309 4628 4802 65
Size: 1183 2061 1319 174 1396
Fragments arranged by size:
2061 1396 1319 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4777 4964 1700
Size: 110 2967 187 2869
Fragments arranged by size:
2967 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 4193 5205 4193
Size: 1012 5121
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6133
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6133
BstXI CCAn_nnnn'nTGG
Cuts at: 2338 2338
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 3046 3259 3463 3830 4215 4232 5684 5770
Size: 1074 213 204 367 385 17 1452 86
Cuts at: 5770 5782 6128 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1074 385 367 346 213 204
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4642
Fragments arranged by size:
4642 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3253 487
Size: 683 56 2027 3367
Fragments arranged by size:
3367 2027 683 56
BtsI GCAGTG_nn'
Cuts at: 2176 2280 3649 4443 4463 2176
Size: 104 1369 794 20 3846
Fragments arranged by size:
3846 1369 794 104 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 3042 3437 3042
Size: 395 5738
DraIII CAC_nnn'GTG
Cuts at: 2711 2711
Size: 6133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3399 3580 4469
Size: 54 1425 50 275 18 1569 181 889
Cuts at: 4469 6098 8
Size: 1629 43
Fragments arranged by size:
1629 1569 1425 889 275 181 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3171 4063 1353
Size: 1818 892 3423
Fragments arranged by size:
3423 1818 892
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4720 5094 5240 387
Size: 1064 138 3131 374 146 1280
Fragments arranged by size:
3131 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3181 3181
Size: 6133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2588 2999 3192 3348 3437 4196
Size: 393 549 407 411 193 156 89 759
Cuts at: 4196 4793 5580 5848 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 759 597 549 411 407 393
268 193 156 89
EcoRI G'AATT_C
Cuts at: 3213 3442 3820 3213
Size: 229 378 5526
Fragments arranged by size:
5526 378 229
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 5280 1678
Size: 3602 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4774 4806 4848 4880 1373
Size: 32 135 32 3202 32 42 32 2626
Fragments arranged by size:
3202 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2050 3477 3723 669
Size: 988 281 112 1427 246 3079
Fragments arranged by size:
3079 1427 988 281 246 112
HindIII A'AGCT_T
Cuts at: 748 2458 748
Size: 1710 4423
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KpnI G_GTAC'C
Cuts at: 2235 3458 2235
Size: 1223 4910
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5247 5431 1305
Size: 3942 184 2007
Fragments arranged by size:
3942 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3401 10
Size: 54 1425 343 1569 2742
Fragments arranged by size:
2742 1569 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2166 4091 4450 4609 518
Size: 648 1000 1925 359 159 2042
Fragments arranged by size:
2042 1925 1000 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2354 2977 3368 3549 4226 5374 5619 1606
Size: 748 623 391 181 677 1148 245 2120
Fragments arranged by size:
2120 1148 748 677 623 391 245 181
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4642
Fragments arranged by size:
4642 939 552
NdeI CA'TA_TG
Cuts at: 387 3289 387
Size: 2902 3231
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 2439 3491 5036 1132
Size: 97 1210 1052 1545 2229
Fragments arranged by size:
2229 1545 1210 1052 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6133
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6133
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PmlI CAC'GTG
Cuts at: 2334 2334
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6133
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PstI C_TGCA'G
Cuts at: 830 3485 830
Size: 2655 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
PvuII CAG'CTG
Cuts at: 2354 2977 2354
Size: 623 5510
SacI G_AGCT'C
Cuts at: 3183 3183
Size: 6133
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SapI GCTCTTCn'nnn_
Cuts at: 3171 3171
Size: 6133
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 4381 6093 4381
Size: 1712 4421
SexAI A'CCwGG_T
Cuts at: 2846 2846
Size: 6133
SfcI C'TryA_G
Cuts at: 826 1045 3481 4616 5297 5488 826
Size: 219 2436 1135 681 191 1471
Fragments arranged by size:
2436 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 3460 1872
Size: 1588 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4237 5138 5400 820
Size: 197 926 2294 901 262 1553
Fragments arranged by size:
2294 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 3491 1132
Size: 2359 3774
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6133
StyI C'CwwG_G
Cuts at: 513 1065 2004 2091 2427 3189 3572 513
Size: 552 939 87 336 762 383 3074
Fragments arranged by size:
3074 939 762 552 383 336 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4160 4177 4330 4515 4960 325
Size: 1255 2580 17 153 185 445 1498
Fragments arranged by size:
2580 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2112 2702 4379
Size: 274 80 33 51 1443 135 590 1677
Cuts at: 4379 6091 96
Size: 1712 138
Fragments arranged by size:
1712 1677 1443 590 274 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6093 6125 6093
Size: 32 6101
Tth111I GACn'n_nGTC
Cuts at: 1316 2045 1316
Size: 729 5404
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 3458 1870
Size: 1588 4545
XmnI GAAnn'nnTTC
Cuts at: 4262 4262
Size: 6133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI XcmI