Restriction map for
pFN21AE3204
(Circular) (Six-base) MAPSORT of: pFN21AE3204.seq Check: 9339 from: 1 to: 6124
pFN21AE3204 6124 bp
With 182 enzymes: SgfI *
March 28, 2012 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2677 2677
Size: 6124
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2009 3467 1937
Size: 72 1458 4594
Fragments arranged by size:
4594 1458 72
Acc65I G'GTAC_C
Cuts at: 3445 3445
Size: 6124
AclI AA'CG_TT
Cuts at: 1563 2212 4251 4624 1563
Size: 649 2039 373 3063
Fragments arranged by size:
3063 2039 649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2292 2300 2621 2702 2930 3098 4187 5571
Size: 660 8 321 81 228 168 1089 1384
Cuts at: 5571 5839 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1089 660 321 268 228 168
81 8
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 5023 6000 1203
Size: 3820 977 1327
Fragments arranged by size:
3820 1327 977
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 4853 4853
Size: 6124
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6124
AlwNI CAG_nnn'CTG
Cuts at: 2821 2936 2965 3867 5439 2821
Size: 115 29 902 1572 3506
Fragments arranged by size:
3506 1572 902 115 29
ApaI G_GGCC'C
Cuts at: 3158 3158
Size: 6124
ApaLI G'TGCA_C
Cuts at: 4181 5337 4181
Size: 1156 4968
ApoI r'AATT_y
Cuts at: 1493 2159 2696 3137 3433 3659 3811 5920 1493
Size: 666 537 441 296 226 152 2109 1697
Fragments arranged by size:
2109 1697 666 537 441 296 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2585 4678 160
Size: 872 1553 2093 1606
Fragments arranged by size:
2093 1606 1553 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 3449 1870
Size: 73 1506 4545
Fragments arranged by size:
4545 1506 73
AvrII C'CTAG_G
Cuts at: 2548 2548
Size: 6124
BamHI G'GATC_C
Cuts at: 3454 3821 3454
Size: 367 5757
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2844 3445 4900 618
Size: 325 217 627 1057 601 1455 1842
Fragments arranged by size:
1842 1455 1057 627 601 325 217
BanII G_rGCy'C
Cuts at: 1391 3158 3422 3443 1391
Size: 1767 264 21 4072
Fragments arranged by size:
4072 1767 264 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2562 2789 928
Size: 971 663 227 4263
Fragments arranged by size:
4263 971 663 227
BbvCI CC'TCA_GC
Cuts at: 3338 3338
Size: 6124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4315 4349 1903
Size: 34 2378 34 3678
Fragments arranged by size:
3678 2378 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3216 4016 5226 3216
Size: 800 1210 4114
Fragments arranged by size:
4114 1210 800
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6124
BfrBI ATG'CAT
Cuts at: 3310 3310
Size: 6124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4735 136
Size: 107 122 71 631 605 3063 1525
Fragments arranged by size:
3063 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2905 6119 2905
Size: 3214 2910
BlpI GC'TnA_GC
Cuts at: 3541 3541
Size: 6124
Bme1580I G_kGCm'C
Cuts at: 1790 2847 3158 4185 5341 1790
Size: 1057 311 1027 1156 2573
Fragments arranged by size:
2573 1156 1057 1027 311
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2055 3416 3855
Size: 416 53 83 399 24 638 1361 439
Cuts at: 3855 4813 442
Size: 958 1753
Fragments arranged by size:
1753 1361 958 638 439 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2133 2217 2843 4784 1239
Size: 884 10 84 626 1941 2579
Fragments arranged by size:
2579 1941 884 626 84 10
Bpu10I CC'TnA_GC
Cuts at: 2053 2183 2759 3338 2053
Size: 130 576 579 4839
Fragments arranged by size:
4839 579 576 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4249 5114 5412 4249
Size: 865 298 4961
Fragments arranged by size:
4961 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3198 4787 882
Size: 617 1699 1589 2219
Fragments arranged by size:
2219 1699 1589 617
BsaAI yAC'GTr
Cuts at: 493 1204 3915 493
Size: 711 2711 2702
Fragments arranged by size:
2711 2702 711
BsaBI GATnn'nnATC
Cuts at: 2538 6118 2538
Size: 3580 2544
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3931 4313 4948 275
Size: 53 83 186 936 2398 382 635 1451
Fragments arranged by size:
2398 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4557 4955 5229 5376 5787 4557
Size: 398 274 147 411 4894
Fragments arranged by size:
4894 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5899
Fragments arranged by size:
5899 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3133 1186
Size: 1947 4177
BseYI C'CCAG_C
Cuts at: 2333 2371 5327 5823 2333
Size: 38 2956 496 2634
Fragments arranged by size:
2956 2634 496 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3383 1163
Size: 573 1647 3904
Fragments arranged by size:
3904 1647 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3574 4335 5363 1540
Size: 275 186 1573 761 1028 2301
Fragments arranged by size:
2301 1573 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4185 4270 5341 4185
Size: 85 1071 4968
Fragments arranged by size:
4968 1071 85
BsmI GAATG_Cn'
Cuts at: 3635 3728 3635
Size: 93 6031
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2847 3158 3422 3443 4185 4270 5341
Size: 399 1057 311 264 21 742 85 1071
Cuts at: 5341 1391
Size: 2174
Fragments arranged by size:
2174 1071 1057 742 399 311 264 85
21
BspEI T'CCGG_A
Cuts at: 5787 5787
Size: 6124
BspHI T'CATG_A
Cuts at: 2344 4013 5743 2344
Size: 1669 1730 2725
Fragments arranged by size:
2725 1730 1669
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 2677 3479 844
Size: 1259 574 802 3489
Fragments arranged by size:
3489 1259 802 574
BsrBI CCG'CTC
Cuts at: 1394 1823 3376 4011 1394
Size: 429 1553 635 3507
Fragments arranged by size:
3507 1553 635 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2439 4619 4793 65
Size: 1183 1191 2180 174 1396
Fragments arranged by size:
2180 1396 1191 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4768 4955 1700
Size: 110 2958 187 2869
Fragments arranged by size:
2958 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 4184 5196 4184
Size: 1012 5112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6124
BstBI TT'CG_AA
Cuts at: 2228 4928 2228
Size: 2700 3424
BstEII G'GTnAC_C
Cuts at: 1184 2294 1184
Size: 1110 5014
BstYI r'GATC_y
Cuts at: 1972 2905 3454 3821 4206 4223 5675 5761 5773
Size: 933 549 367 385 17 1452 86 12
Cuts at: 5773 6119 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 933 549 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2410 513
Size: 552 1345 4227
Fragments arranged by size:
4227 1345 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5385
Fragments arranged by size:
5385 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 3640 4434 4454 2245
Size: 1395 794 20 3915
Fragments arranged by size:
3915 1395 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 2778 3428 2778
Size: 650 5474
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2413 2746 3571
Size: 54 1425 50 275 18 583 333 825
Cuts at: 3571 4460 6089 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 825 583 333 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2562 3104 3177 4054 1353
Size: 671 538 542 73 877 3423
Fragments arranged by size:
3423 877 671 542 538 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4711 5085 5231 387
Size: 1064 138 3122 374 146 1280
Fragments arranged by size:
3122 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2133 2217 2292 2300 2621 2702
Size: 393 491 10 84 75 8 321 81
Cuts at: 2702 2843 2930 3098 4187 4784 5571 5839 1239
Size: 141 87 168 1089 597 787 268 1524
Fragments arranged by size:
1524 1089 787 597 491 393 321 268
168 141 87 84 81 75 10 8
EcoNI CCTnn'n_nnAGG
Cuts at: 3148 3148
Size: 6124
EcoO109I rG'GnC_Cy
Cuts at: 2057 3154 3155 2057
Size: 1097 1 5026
Fragments arranged by size:
5026 1097 1
EcoRI G'AATT_C
Cuts at: 3137 3433 3811 3137
Size: 296 378 5450
Fragments arranged by size:
5450 378 296
FspI TGC'GCA
Cuts at: 4630 4630
Size: 6124
HaeII r_GCGC'y
Cuts at: 1678 5271 1678
Size: 3593 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2776 2808 3009 3041 3176
Size: 32 135 32 1204 32 201 32 135
Cuts at: 3176 3208 4765 4797 4839 4871 1373
Size: 32 1557 32 42 32 2626
Fragments arranged by size:
2626 1557 1204 201 135 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2920 3468 3714 669
Size: 988 281 982 548 246 3079
Fragments arranged by size:
3079 988 982 548 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6124
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KpnI G_GTAC'C
Cuts at: 3449 3449
Size: 6124
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5238 5422 1305
Size: 3933 184 2007
Fragments arranged by size:
3933 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2415 2748 10
Size: 54 1425 343 583 333 3386
Fragments arranged by size:
3386 1425 583 343 333 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2816 4082 4441 4600 518
Size: 648 1650 1266 359 159 2042
Fragments arranged by size:
2042 1650 1266 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3171 3382 3540 4217 5365 5610 1606
Size: 1565 211 158 677 1148 245 2120
Fragments arranged by size:
2120 1565 1148 677 245 211 158
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5572
NdeI CA'TA_TG
Cuts at: 387 3004 387
Size: 2617 3507
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6124
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NsiI A_TGCA'T
Cuts at: 3312 3312
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 3482 5027 1132
Size: 97 2253 1545 2229
Fragments arranged by size:
2253 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6124
PciI A'CATG_T
Cuts at: 5023 5023
Size: 6124
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6124
PfoI T'CCnGG_A
Cuts at: 2100 2100
Size: 6124
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 5706 5738 4193
Size: 32 1481 32 4579
Fragments arranged by size:
4579 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2057 2057
Size: 6124
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PspOMI G'GGCC_C
Cuts at: 3154 3154
Size: 6124
PstI C_TGCA'G
Cuts at: 830 2278 3476 830
Size: 1448 1198 3478
Fragments arranged by size:
3478 1448 1198
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
PvuII CAG'CTG
Cuts at: 3382 3382
Size: 6124
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 4372 6084 4372
Size: 1712 4412
SfcI C'TryA_G
Cuts at: 826 1045 2274 3250 3472 4607 5288 5479 826
Size: 219 1229 976 222 1135 681 191 1471
Fragments arranged by size:
1471 1229 1135 976 681 222 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 3451 1872
Size: 1579 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4228 5129 5391 820
Size: 197 926 2285 901 262 1553
Fragments arranged by size:
2285 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 3482 1132
Size: 2350 3774
SspI AAT'ATT
Cuts at: 5 52 2498 4048 5
Size: 47 2446 1550 2081
Fragments arranged by size:
2446 2081 1550 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6124
StyI C'CwwG_G
Cuts at: 513 1065 2548 2749 2865 3563 513
Size: 552 1483 201 116 698 3074
Fragments arranged by size:
3074 1483 698 552 201 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3119 4151 4168 4321 4506 4951 325
Size: 1255 1539 1032 17 153 185 445 1498
Fragments arranged by size:
1539 1498 1255 1032 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3216 4370 6082
Size: 274 80 33 51 1443 1239 1154 1712
Cuts at: 6082 96
Size: 138
Fragments arranged by size:
1712 1443 1239 1154 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2463 2495 6084 6116 2463
Size: 32 3589 32 2471
Fragments arranged by size:
3589 2471 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6124
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3094 3094
Size: 6124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 3449 1870
Size: 1579 4545
XmnI GAAnn'nnTTC
Cuts at: 4253 4253
Size: 6124
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI