(Circular) (Six-base) MAPSORT of: pFN21AE3193.seq Check: 2786 from: 1 to: 6052 pFN21AE3193 6052 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2551 3066 2551 Size: 515 5537 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3862 278 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2724 2991 3395 1937 Size: 787 267 404 4594 Fragments arranged by size: 4594 787 404 267 Acc65I G'GTAC_C Cuts at: 3373 3373 Size: 6052 AclI AA'CG_TT Cuts at: 1563 4179 4552 1563 Size: 2616 373 3063 Fragments arranged by size: 3063 2616 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2718 4115 5499 5767 1632 Size: 1086 1397 1384 268 1917 Fragments arranged by size: 1917 1397 1384 1086 268 AfeI AGC'GCT Cuts at: 2439 2439 Size: 6052 AflII C'TTAA_G Cuts at: 820 1017 2831 820 Size: 197 1814 4041 Fragments arranged by size: 4041 1814 197 AflIII A'CryG_T Cuts at: 1203 4951 5928 1203 Size: 3748 977 1327 Fragments arranged by size: 3748 1327 977 AgeI A'CCGG_T Cuts at: 3349 4883 3349 Size: 1534 4518 AhdI GACnn_n'nnGTC Cuts at: 4781 4781 Size: 6052 AleI CACnn'nnGTG Cuts at: 1166 3035 1166 Size: 1869 4183 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2573 1184 Size: 1389 4663 AlwNI CAG_nnn'CTG Cuts at: 2296 3000 3058 3160 3795 5367 2296 Size: 704 58 102 635 1572 2981 Fragments arranged by size: 2981 1572 704 635 102 58 ApaI G_GGCC'C Cuts at: 2208 2266 2208 Size: 58 5994 ApaLI G'TGCA_C Cuts at: 4109 5265 4109 Size: 1156 4896 ApoI r'AATT_y Cuts at: 1493 3361 3587 3739 5848 1493 Size: 1868 226 152 2109 1697 Fragments arranged by size: 2109 1868 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4606 160 Size: 872 3574 1606 Fragments arranged by size: 3574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 AvaI C'yCGr_G Cuts at: 1870 1943 2054 2265 2362 2444 2661 2703 3377 Size: 73 111 211 97 82 217 42 674 Cuts at: 3377 1870 Size: 4545 Fragments arranged by size: 4545 674 217 211 111 97 82 73 42 AvrII C'CTAG_G Cuts at: 2143 2143 Size: 6052 BamHI G'GATC_C Cuts at: 3382 3749 3382 Size: 367 5685 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2147 2331 2456 2470 2614 Size: 325 217 627 360 184 125 14 144 Cuts at: 2614 3373 4828 618 Size: 759 1455 1842 Fragments arranged by size: 1842 1455 759 627 360 325 217 184 144 125 14 BanII G_rGCy'C Cuts at: 1391 2208 2266 2598 3371 1391 Size: 817 58 332 773 4072 Fragments arranged by size: 4072 817 773 332 58 BbeI G_GCGC'C Cuts at: 2151 2460 2151 Size: 309 5743 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2632 3188 928 Size: 971 733 556 3792 Fragments arranged by size: 3792 971 733 556 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4243 4277 1903 Size: 34 2306 34 3678 Fragments arranged by size: 3678 2306 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3944 5154 3944 Size: 1210 4842 BclI T'GATC_A Cuts at: 1548 1548 Size: 6052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2367 2376 2663 Size: 107 122 71 631 605 695 9 287 Cuts at: 2663 4663 136 Size: 2000 1525 Fragments arranged by size: 2000 1525 695 631 605 287 122 107 71 9 BglII A'GATC_T Cuts at: 2883 6047 2883 Size: 3164 2888 BlpI GC'TnA_GC Cuts at: 3469 3469 Size: 6052 Bme1580I G_kGCm'C Cuts at: 1790 2208 2238 2266 2586 2735 4113 5269 1790 Size: 418 30 28 320 149 1378 1156 2573 Fragments arranged by size: 2573 1378 1156 418 320 149 30 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6052 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2622 2806 3783 Size: 416 53 83 399 24 1205 184 977 Cuts at: 3783 4741 442 Size: 958 1753 Fragments arranged by size: 1753 1205 977 958 416 399 184 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6052 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2701 2701 Size: 6052 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 3035 3277 4712 1239 Size: 890 906 242 1435 2579 Fragments arranged by size: 2579 1435 906 890 242 Bpu10I CC'TnA_GC Cuts at: 2342 2342 Size: 6052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3224 4177 5042 5340 3224 Size: 953 865 298 3936 Fragments arranged by size: 3936 953 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3053 4715 882 Size: 617 1554 1662 2219 Fragments arranged by size: 2219 1662 1554 617 BsaAI yAC'GTr Cuts at: 493 1204 3843 493 Size: 711 2639 2702 Fragments arranged by size: 2702 2639 711 BsaBI GATnn'nnATC Cuts at: 6046 6046 Size: 6052 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 2154 2172 2250 Size: 53 83 186 936 615 6 18 78 Cuts at: 2250 2457 3859 4241 4876 275 Size: 207 1402 382 635 1451 Fragments arranged by size: 1451 1402 936 635 615 382 207 186 83 78 53 18 6 BsaWI w'CCGG_w Cuts at: 3349 4485 4883 5157 5304 5715 3349 Size: 1136 398 274 147 411 3686 Fragments arranged by size: 3686 1136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5827 Fragments arranged by size: 5827 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2690 3158 1186 Size: 1504 468 4080 Fragments arranged by size: 4080 1504 468 BseYI C'CCAG_C Cuts at: 2075 2200 2452 3064 5255 5751 2075 Size: 125 252 612 2191 496 2376 Fragments arranged by size: 2376 2191 612 496 252 125 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2107 2270 2285 2588 1163 Size: 573 371 163 15 303 4627 Fragments arranged by size: 4627 573 371 303 163 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3502 4263 5291 1540 Size: 275 186 1501 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4113 4198 5269 4113 Size: 85 1071 4896 Fragments arranged by size: 4896 1071 85 BsmI GAATG_Cn' Cuts at: 3563 3656 3563 Size: 93 5959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2238 2266 2586 2598 2735 3371 Size: 399 418 30 28 320 12 137 636 Cuts at: 3371 4113 4198 5269 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 636 418 399 320 137 85 30 28 12 BspEI T'CCGG_A Cuts at: 5715 5715 Size: 6052 BspHI T'CATG_A Cuts at: 3941 5671 3941 Size: 1730 4322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2551 3066 3108 3407 844 Size: 1707 515 42 299 3489 Fragments arranged by size: 3489 1707 515 299 42 BsrBI CCG'CTC Cuts at: 1394 1823 3939 1394 Size: 429 2116 3507 Fragments arranged by size: 3507 2116 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4547 4721 65 Size: 1183 3299 174 1396 Fragments arranged by size: 3299 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2019 2252 2375 3349 4696 4883 1700 Size: 110 209 233 123 974 1347 187 2869 Fragments arranged by size: 2869 1347 974 233 209 187 123 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6052 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6052 BssSI C'ACGA_G Cuts at: 4112 5124 4112 Size: 1012 5040 BstAPI GCAn_nnn'nTGC Cuts at: 794 2308 2440 3160 794 Size: 1514 132 720 3686 Fragments arranged by size: 3686 1514 720 132 BstBI TT'CG_AA Cuts at: 4856 4856 Size: 6052 BstEII G'GTnAC_C Cuts at: 1184 2806 1184 Size: 1622 4430 BstXI CCAn_nnnn'nTGG Cuts at: 2635 2635 Size: 6052 BstYI r'GATC_y Cuts at: 1972 2742 2883 3382 3749 4134 4151 5603 5689 Size: 770 141 499 367 385 17 1452 86 Cuts at: 5689 5701 6047 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 770 499 385 367 346 141 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3141 3141 Size: 6052 BtgI C'CryG_G Cuts at: 513 1065 2004 2082 2314 2634 2790 2802 513 Size: 552 939 78 232 320 156 12 3763 Fragments arranged by size: 3763 939 552 320 232 156 78 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5313 Fragments arranged by size: 5313 683 56 BtsI GCAGTG_nn' Cuts at: 3568 4362 4382 3568 Size: 794 20 5238 Fragments arranged by size: 5238 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6052 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6017 DraI TTT'AAA Cuts at: 3356 3356 Size: 6052 DraIII CAC_nnn'GTG Cuts at: 2611 3079 2611 Size: 468 5584 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5059 809 Size: 507 3743 1802 Fragments arranged by size: 3743 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2368 2512 2869 Size: 54 1425 50 275 18 538 144 357 Cuts at: 2869 3012 3089 3499 4388 6017 8 Size: 143 77 410 889 1629 43 Fragments arranged by size: 1629 1425 889 538 410 357 275 144 143 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3499 1537 Size: 275 1687 4090 Fragments arranged by size: 4090 1687 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2908 3982 1353 Size: 1555 1074 3423 Fragments arranged by size: 3423 1555 1074 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2079 4639 5013 5159 387 Size: 1064 138 490 2560 374 146 1280 Fragments arranged by size: 2560 1280 1064 490 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2718 3035 3277 4115 4712 5499 Size: 393 497 589 317 242 838 597 787 Cuts at: 5499 5767 1239 Size: 268 1524 Fragments arranged by size: 1524 838 787 597 589 497 393 317 268 242 EcoNI CCTnn'n_nnAGG Cuts at: 2534 2534 Size: 6052 EcoO109I rG'GnC_Cy Cuts at: 2204 2205 2262 3097 3304 2204 Size: 1 57 835 207 4952 Fragments arranged by size: 4952 835 207 57 1 EcoRI G'AATT_C Cuts at: 3361 3739 3361 Size: 378 5674 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3218 3218 Size: 6052 FspI TGC'GCA Cuts at: 2572 4558 2572 Size: 1986 4066 HaeII r_GCGC'y Cuts at: 1678 2151 2198 2296 2441 2460 2530 5199 1678 Size: 473 47 98 145 19 70 2669 2531 Fragments arranged by size: 2669 2531 473 145 98 70 47 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3031 3063 3244 3276 3288 Size: 32 135 32 1459 32 181 32 12 Cuts at: 3288 3320 4693 4725 4767 4799 1373 Size: 32 1373 32 42 32 2626 Fragments arranged by size: 2626 1459 1373 181 135 42 32 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 2725 3396 3642 669 Size: 988 281 787 671 246 3079 Fragments arranged by size: 3079 988 787 671 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6052 HpaI GTT'AAC Cuts at: 3642 3642 Size: 6052 KasI G'GCGC_C Cuts at: 2147 2456 2147 Size: 309 5743 KpnI G_GTAC'C Cuts at: 3377 3377 Size: 6052 MfeI C'AATT_G Cuts at: 3651 3651 Size: 6052 MluI A'CGCG_T Cuts at: 5928 5928 Size: 6052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5166 5350 1305 Size: 3861 184 2007 Fragments arranged by size: 3861 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2871 3091 10 Size: 54 1425 343 1039 220 2971 Fragments arranged by size: 2971 1425 1039 343 220 54 MslI CAynn'nnrTG Cuts at: 518 1166 3035 4010 4369 4528 518 Size: 648 1869 975 359 159 2042 Fragments arranged by size: 2042 1869 975 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2308 2316 2329 2338 2518 2602 3250 Size: 444 258 8 13 9 180 84 648 Cuts at: 3250 3468 4145 5293 5538 1606 Size: 218 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 648 444 258 245 218 180 84 13 9 8 NaeI GCC'GGC Cuts at: 2021 2254 2377 2021 Size: 233 123 5696 Fragments arranged by size: 5696 233 123 NarI GG'CG_CC Cuts at: 2148 2457 2148 Size: 309 5743 NcoI C'CATG_G Cuts at: 513 1065 2004 2082 513 Size: 552 939 78 4483 Fragments arranged by size: 4483 939 552 78 NdeI CA'TA_TG Cuts at: 387 387 Size: 6052 NgoMIV G'CCGG_C Cuts at: 2019 2252 2375 2019 Size: 233 123 5696 Fragments arranged by size: 5696 233 123 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6052 NotI GC'GGCC_GC Cuts at: 3499 3499 Size: 6052 NspI r_CATG'y Cuts at: 1132 1229 3410 4955 1132 Size: 97 2181 1545 2229 Fragments arranged by size: 2229 2181 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6052 PciI A'CATG_T Cuts at: 4951 4951 Size: 6052 PflMI CCAn_nnn'nTGG Cuts at: 1108 2818 3253 1108 Size: 1710 435 3907 Fragments arranged by size: 3907 1710 435 PfoI T'CCnGG_A Cuts at: 2106 2589 2643 2106 Size: 483 54 5515 Fragments arranged by size: 5515 483 54 PmeI GTTT'AAAC Cuts at: 3356 3356 Size: 6052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4121 4153 5634 5666 4121 Size: 32 1481 32 4507 Fragments arranged by size: 4507 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3097 3304 3097 Size: 207 5845 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6052 PsiI TTA'TAA Cuts at: 3622 3622 Size: 6052 PspOMI G'GGCC_C Cuts at: 2204 2262 2204 Size: 58 5994 PspXI vC'TCGA_Gb Cuts at: 2444 2444 Size: 6052 PstI C_TGCA'G Cuts at: 830 2562 3165 3312 3404 830 Size: 1732 603 147 92 3478 Fragments arranged by size: 3478 1732 603 147 92 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6052 PvuII CAG'CTG Cuts at: 2308 2338 2518 2308 Size: 30 180 5842 Fragments arranged by size: 5842 180 30 RsrII CG'GwC_CG Cuts at: 2647 2647 Size: 6052 SacII CC_GC'GG Cuts at: 2317 2317 Size: 6052 SalI G'TCGA_C Cuts at: 1936 2723 3394 1936 Size: 787 671 4594 Fragments arranged by size: 4594 787 671 SapI GCTCTTCn'nnn_ Cuts at: 2908 2908 Size: 6052 SbfI CC_TGCA'GG Cuts at: 3404 3404 Size: 6052 ScaI AGT'ACT Cuts at: 4300 6012 4300 Size: 1712 4340 SexAI A'CCwGG_T Cuts at: 2718 2718 Size: 6052 SfcI C'TryA_G Cuts at: 826 1045 2558 3161 3308 3400 4535 5216 5407 Size: 219 1513 603 147 92 1135 681 191 Cuts at: 5407 826 Size: 1471 Fragments arranged by size: 1513 1471 1135 681 603 219 191 147 92 SfoI GGC'GCC Cuts at: 2149 2458 2149 Size: 309 5743 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6052 SmaI CCC'GGG Cuts at: 1872 2267 2364 2663 2705 3379 1872 Size: 395 97 299 42 674 4545 Fragments arranged by size: 4545 674 395 299 97 42 SmlI C'TyrA_G Cuts at: 820 1017 1943 2444 2831 3203 4156 5057 5319 Size: 197 926 501 387 372 953 901 262 Cuts at: 5319 820 Size: 1553 Fragments arranged by size: 1553 953 926 901 501 387 372 262 197 SnaBI TAC'GTA Cuts at: 493 3843 493 Size: 3350 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6052 SphI G_CATG'C Cuts at: 1132 3410 1132 Size: 2278 3774 SrfI GCCC'GGGC Cuts at: 2364 2663 2705 2364 Size: 299 42 5711 Fragments arranged by size: 5711 299 42 SspI AAT'ATT Cuts at: 5 52 3976 5 Size: 47 3924 2081 Fragments arranged by size: 3924 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6052 StyI C'CwwG_G Cuts at: 513 1065 2004 2082 2143 3491 513 Size: 552 939 78 61 1348 3074 Fragments arranged by size: 3074 1348 939 552 78 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4079 4096 4249 4434 4879 325 Size: 1255 2499 17 153 185 445 1498 Fragments arranged by size: 2499 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4298 6010 96 Size: 274 80 33 51 1443 2321 1712 138 Fragments arranged by size: 2321 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3121 3153 6012 6044 3121 Size: 32 2859 32 3129 Fragments arranged by size: 3129 2859 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2720 2812 1316 Size: 1404 92 4556 Fragments arranged by size: 4556 1404 92 XbaI T'CTAG_A Cuts at: 3388 3388 Size: 6052 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2779 2779 Size: 6052 XhoI C'TCGA_G Cuts at: 1943 2444 1943 Size: 501 5551 XmaI C'CCGG_G Cuts at: 1870 2265 2362 2661 2703 3377 1870 Size: 395 97 299 42 674 4545 Fragments arranged by size: 4545 674 395 299 97 42 XmnI GAAnn'nnTTC Cuts at: 4181 4181 Size: 6052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3860 276 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI SacI SanDI SfiI SwaI