Restriction map for
pFN21AE3193
(Circular) (Six-base) MAPSORT of: pFN21AE3193.seq Check: 2786 from: 1 to: 6052
pFN21AE3193 6052 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2551 3066 2551
Size: 515 5537
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3862 278
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2724 2991 3395 1937
Size: 787 267 404 4594
Fragments arranged by size:
4594 787 404 267
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2718 4115 5499 5767 1632
Size: 1086 1397 1384 268 1917
Fragments arranged by size:
1917 1397 1384 1086 268
AfeI AGC'GCT
Cuts at: 2439 2439
Size: 6052
AflII C'TTAA_G
Cuts at: 820 1017 2831 820
Size: 197 1814 4041
Fragments arranged by size:
4041 1814 197
AflIII A'CryG_T
Cuts at: 1203 4951 5928 1203
Size: 3748 977 1327
Fragments arranged by size:
3748 1327 977
AgeI A'CCGG_T
Cuts at: 3349 4883 3349
Size: 1534 4518
AhdI GACnn_n'nnGTC
Cuts at: 4781 4781
Size: 6052
AleI CACnn'nnGTG
Cuts at: 1166 3035 1166
Size: 1869 4183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2573 1184
Size: 1389 4663
AlwNI CAG_nnn'CTG
Cuts at: 2296 3000 3058 3160 3795 5367 2296
Size: 704 58 102 635 1572 2981
Fragments arranged by size:
2981 1572 704 635 102 58
ApaI G_GGCC'C
Cuts at: 2208 2266 2208
Size: 58 5994
ApaLI G'TGCA_C
Cuts at: 4109 5265 4109
Size: 1156 4896
ApoI r'AATT_y
Cuts at: 1493 3361 3587 3739 5848 1493
Size: 1868 226 152 2109 1697
Fragments arranged by size:
2109 1868 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2265 2362 2444 2661 2703 3377
Size: 73 111 211 97 82 217 42 674
Cuts at: 3377 1870
Size: 4545
Fragments arranged by size:
4545 674 217 211 111 97 82 73
42
AvrII C'CTAG_G
Cuts at: 2143 2143
Size: 6052
BamHI G'GATC_C
Cuts at: 3382 3749 3382
Size: 367 5685
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 2331 2456 2470 2614
Size: 325 217 627 360 184 125 14 144
Cuts at: 2614 3373 4828 618
Size: 759 1455 1842
Fragments arranged by size:
1842 1455 759 627 360 325 217 184
144 125 14
BanII G_rGCy'C
Cuts at: 1391 2208 2266 2598 3371 1391
Size: 817 58 332 773 4072
Fragments arranged by size:
4072 817 773 332 58
BbeI G_GCGC'C
Cuts at: 2151 2460 2151
Size: 309 5743
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2632 3188 928
Size: 971 733 556 3792
Fragments arranged by size:
3792 971 733 556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4243 4277 1903
Size: 34 2306 34 3678
Fragments arranged by size:
3678 2306 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3944 5154 3944
Size: 1210 4842
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2367 2376 2663
Size: 107 122 71 631 605 695 9 287
Cuts at: 2663 4663 136
Size: 2000 1525
Fragments arranged by size:
2000 1525 695 631 605 287 122 107
71 9
BglII A'GATC_T
Cuts at: 2883 6047 2883
Size: 3164 2888
BlpI GC'TnA_GC
Cuts at: 3469 3469
Size: 6052
Bme1580I G_kGCm'C
Cuts at: 1790 2208 2238 2266 2586 2735 4113 5269 1790
Size: 418 30 28 320 149 1378 1156 2573
Fragments arranged by size:
2573 1378 1156 418 320 149 30 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6052
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2622 2806 3783
Size: 416 53 83 399 24 1205 184 977
Cuts at: 3783 4741 442
Size: 958 1753
Fragments arranged by size:
1753 1205 977 958 416 399 184 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6052
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2701 2701
Size: 6052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 3035 3277 4712 1239
Size: 890 906 242 1435 2579
Fragments arranged by size:
2579 1435 906 890 242
Bpu10I CC'TnA_GC
Cuts at: 2342 2342
Size: 6052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3224 4177 5042 5340 3224
Size: 953 865 298 3936
Fragments arranged by size:
3936 953 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3053 4715 882
Size: 617 1554 1662 2219
Fragments arranged by size:
2219 1662 1554 617
BsaAI yAC'GTr
Cuts at: 493 1204 3843 493
Size: 711 2639 2702
Fragments arranged by size:
2702 2639 711
BsaBI GATnn'nnATC
Cuts at: 6046 6046
Size: 6052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 2154 2172 2250
Size: 53 83 186 936 615 6 18 78
Cuts at: 2250 2457 3859 4241 4876 275
Size: 207 1402 382 635 1451
Fragments arranged by size:
1451 1402 936 635 615 382 207 186
83 78 53 18 6
BsaWI w'CCGG_w
Cuts at: 3349 4485 4883 5157 5304 5715 3349
Size: 1136 398 274 147 411 3686
Fragments arranged by size:
3686 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5827
Fragments arranged by size:
5827 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 3158 1186
Size: 1504 468 4080
Fragments arranged by size:
4080 1504 468
BseYI C'CCAG_C
Cuts at: 2075 2200 2452 3064 5255 5751 2075
Size: 125 252 612 2191 496 2376
Fragments arranged by size:
2376 2191 612 496 252 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2107 2270 2285 2588 1163
Size: 573 371 163 15 303 4627
Fragments arranged by size:
4627 573 371 303 163 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4263 5291 1540
Size: 275 186 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4113 4198 5269 4113
Size: 85 1071 4896
Fragments arranged by size:
4896 1071 85
BsmI GAATG_Cn'
Cuts at: 3563 3656 3563
Size: 93 5959
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2238 2266 2586 2598 2735 3371
Size: 399 418 30 28 320 12 137 636
Cuts at: 3371 4113 4198 5269 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 636 418 399 320 137
85 30 28 12
BspEI T'CCGG_A
Cuts at: 5715 5715
Size: 6052
BspHI T'CATG_A
Cuts at: 3941 5671 3941
Size: 1730 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2551 3066 3108 3407 844
Size: 1707 515 42 299 3489
Fragments arranged by size:
3489 1707 515 299 42
BsrBI CCG'CTC
Cuts at: 1394 1823 3939 1394
Size: 429 2116 3507
Fragments arranged by size:
3507 2116 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4547 4721 65
Size: 1183 3299 174 1396
Fragments arranged by size:
3299 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2019 2252 2375 3349 4696 4883 1700
Size: 110 209 233 123 974 1347 187 2869
Fragments arranged by size:
2869 1347 974 233 209 187 123 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6052
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 4112 5124 4112
Size: 1012 5040
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2308 2440 3160 794
Size: 1514 132 720 3686
Fragments arranged by size:
3686 1514 720 132
BstBI TT'CG_AA
Cuts at: 4856 4856
Size: 6052
BstEII G'GTnAC_C
Cuts at: 1184 2806 1184
Size: 1622 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 2635 2635
Size: 6052
BstYI r'GATC_y
Cuts at: 1972 2742 2883 3382 3749 4134 4151 5603 5689
Size: 770 141 499 367 385 17 1452 86
Cuts at: 5689 5701 6047 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 770 499 385 367 346 141
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3141 3141
Size: 6052
BtgI C'CryG_G
Cuts at: 513 1065 2004 2082 2314 2634 2790 2802 513
Size: 552 939 78 232 320 156 12 3763
Fragments arranged by size:
3763 939 552 320 232 156 78 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5313
Fragments arranged by size:
5313 683 56
BtsI GCAGTG_nn'
Cuts at: 3568 4362 4382 3568
Size: 794 20 5238
Fragments arranged by size:
5238 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6017
DraI TTT'AAA
Cuts at: 3356 3356
Size: 6052
DraIII CAC_nnn'GTG
Cuts at: 2611 3079 2611
Size: 468 5584
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5059 809
Size: 507 3743 1802
Fragments arranged by size:
3743 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2368 2512 2869
Size: 54 1425 50 275 18 538 144 357
Cuts at: 2869 3012 3089 3499 4388 6017 8
Size: 143 77 410 889 1629 43
Fragments arranged by size:
1629 1425 889 538 410 357 275 144
143 77 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2908 3982 1353
Size: 1555 1074 3423
Fragments arranged by size:
3423 1555 1074
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 4639 5013 5159 387
Size: 1064 138 490 2560 374 146 1280
Fragments arranged by size:
2560 1280 1064 490 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2718 3035 3277 4115 4712 5499
Size: 393 497 589 317 242 838 597 787
Cuts at: 5499 5767 1239
Size: 268 1524
Fragments arranged by size:
1524 838 787 597 589 497 393 317
268 242
EcoNI CCTnn'n_nnAGG
Cuts at: 2534 2534
Size: 6052
EcoO109I rG'GnC_Cy
Cuts at: 2204 2205 2262 3097 3304 2204
Size: 1 57 835 207 4952
Fragments arranged by size:
4952 835 207 57 1
EcoRI G'AATT_C
Cuts at: 3361 3739 3361
Size: 378 5674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3218 3218
Size: 6052
FspI TGC'GCA
Cuts at: 2572 4558 2572
Size: 1986 4066
HaeII r_GCGC'y
Cuts at: 1678 2151 2198 2296 2441 2460 2530 5199 1678
Size: 473 47 98 145 19 70 2669 2531
Fragments arranged by size:
2669 2531 473 145 98 70 47 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3031 3063 3244 3276 3288
Size: 32 135 32 1459 32 181 32 12
Cuts at: 3288 3320 4693 4725 4767 4799 1373
Size: 32 1373 32 42 32 2626
Fragments arranged by size:
2626 1459 1373 181 135 42 32 32
32 32 32 32 32 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2725 3396 3642 669
Size: 988 281 787 671 246 3079
Fragments arranged by size:
3079 988 787 671 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6052
HpaI GTT'AAC
Cuts at: 3642 3642
Size: 6052
KasI G'GCGC_C
Cuts at: 2147 2456 2147
Size: 309 5743
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 3651 3651
Size: 6052
MluI A'CGCG_T
Cuts at: 5928 5928
Size: 6052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5166 5350 1305
Size: 3861 184 2007
Fragments arranged by size:
3861 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2871 3091 10
Size: 54 1425 343 1039 220 2971
Fragments arranged by size:
2971 1425 1039 343 220 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3035 4010 4369 4528 518
Size: 648 1869 975 359 159 2042
Fragments arranged by size:
2042 1869 975 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2308 2316 2329 2338 2518 2602 3250
Size: 444 258 8 13 9 180 84 648
Cuts at: 3250 3468 4145 5293 5538 1606
Size: 218 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 648 444 258 245 218
180 84 13 9 8
NaeI GCC'GGC
Cuts at: 2021 2254 2377 2021
Size: 233 123 5696
Fragments arranged by size:
5696 233 123
NarI GG'CG_CC
Cuts at: 2148 2457 2148
Size: 309 5743
NcoI C'CATG_G
Cuts at: 513 1065 2004 2082 513
Size: 552 939 78 4483
Fragments arranged by size:
4483 939 552 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6052
NgoMIV G'CCGG_C
Cuts at: 2019 2252 2375 2019
Size: 233 123 5696
Fragments arranged by size:
5696 233 123
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6052
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 3410 4955 1132
Size: 97 2181 1545 2229
Fragments arranged by size:
2229 2181 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6052
PciI A'CATG_T
Cuts at: 4951 4951
Size: 6052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2818 3253 1108
Size: 1710 435 3907
Fragments arranged by size:
3907 1710 435
PfoI T'CCnGG_A
Cuts at: 2106 2589 2643 2106
Size: 483 54 5515
Fragments arranged by size:
5515 483 54
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 5634 5666 4121
Size: 32 1481 32 4507
Fragments arranged by size:
4507 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3097 3304 3097
Size: 207 5845
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6052
PsiI TTA'TAA
Cuts at: 3622 3622
Size: 6052
PspOMI G'GGCC_C
Cuts at: 2204 2262 2204
Size: 58 5994
PspXI vC'TCGA_Gb
Cuts at: 2444 2444
Size: 6052
PstI C_TGCA'G
Cuts at: 830 2562 3165 3312 3404 830
Size: 1732 603 147 92 3478
Fragments arranged by size:
3478 1732 603 147 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
PvuII CAG'CTG
Cuts at: 2308 2338 2518 2308
Size: 30 180 5842
Fragments arranged by size:
5842 180 30
RsrII CG'GwC_CG
Cuts at: 2647 2647
Size: 6052
SacII CC_GC'GG
Cuts at: 2317 2317
Size: 6052
SalI G'TCGA_C
Cuts at: 1936 2723 3394 1936
Size: 787 671 4594
Fragments arranged by size:
4594 787 671
SapI GCTCTTCn'nnn_
Cuts at: 2908 2908
Size: 6052
SbfI CC_TGCA'GG
Cuts at: 3404 3404
Size: 6052
ScaI AGT'ACT
Cuts at: 4300 6012 4300
Size: 1712 4340
SexAI A'CCwGG_T
Cuts at: 2718 2718
Size: 6052
SfcI C'TryA_G
Cuts at: 826 1045 2558 3161 3308 3400 4535 5216 5407
Size: 219 1513 603 147 92 1135 681 191
Cuts at: 5407 826
Size: 1471
Fragments arranged by size:
1513 1471 1135 681 603 219 191 147
92
SfoI GGC'GCC
Cuts at: 2149 2458 2149
Size: 309 5743
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6052
SmaI CCC'GGG
Cuts at: 1872 2267 2364 2663 2705 3379 1872
Size: 395 97 299 42 674 4545
Fragments arranged by size:
4545 674 395 299 97 42
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 2831 3203 4156 5057 5319
Size: 197 926 501 387 372 953 901 262
Cuts at: 5319 820
Size: 1553
Fragments arranged by size:
1553 953 926 901 501 387 372 262
197
SnaBI TAC'GTA
Cuts at: 493 3843 493
Size: 3350 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 3410 1132
Size: 2278 3774
SrfI GCCC'GGGC
Cuts at: 2364 2663 2705 2364
Size: 299 42 5711
Fragments arranged by size:
5711 299 42
SspI AAT'ATT
Cuts at: 5 52 3976 5
Size: 47 3924 2081
Fragments arranged by size:
3924 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6052
StyI C'CwwG_G
Cuts at: 513 1065 2004 2082 2143 3491 513
Size: 552 939 78 61 1348 3074
Fragments arranged by size:
3074 1348 939 552 78 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4079 4096 4249 4434 4879 325
Size: 1255 2499 17 153 185 445 1498
Fragments arranged by size:
2499 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4298 6010 96
Size: 274 80 33 51 1443 2321 1712 138
Fragments arranged by size:
2321 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3121 3153 6012 6044 3121
Size: 32 2859 32 3129
Fragments arranged by size:
3129 2859 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2720 2812 1316
Size: 1404 92 4556
Fragments arranged by size:
4556 1404 92
XbaI T'CTAG_A
Cuts at: 3388 3388
Size: 6052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2779 2779
Size: 6052
XhoI C'TCGA_G
Cuts at: 1943 2444 1943
Size: 501 5551
XmaI C'CCGG_G
Cuts at: 1870 2265 2362 2661 2703 3377 1870
Size: 395 97 299 42 674 4545
Fragments arranged by size:
4545 674 395 299 97 42
XmnI GAAnn'nnTTC
Cuts at: 4181 4181
Size: 6052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3860 276
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I
EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI
PsrI SacI SanDI SfiI SwaI