Restriction map for
pFN21AE3192
(Circular) (Six-base) MAPSORT of: pFN21AE3192.seq Check: 1287 from: 1 to: 6046
pFN21AE3192 6046 bp
With 182 enzymes: SgfI *
March 28, 2012 12:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3856 278
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3389 1937
Size: 1452 4594
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2967 4109 5493 5761 1632
Size: 1335 1142 1384 268 1917
Fragments arranged by size:
1917 1384 1335 1142 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 2757 4945 5922 1203
Size: 1554 2188 977 1327
Fragments arranged by size:
2188 1554 1327 977
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6046
AlwNI CAG_nnn'CTG
Cuts at: 2045 2736 3789 5361 2045
Size: 691 1053 1572 2730
Fragments arranged by size:
2730 1572 1053 691
ApaLI G'TGCA_C
Cuts at: 4103 5259 4103
Size: 1156 4890
ApoI r'AATT_y
Cuts at: 1493 3355 3581 3733 5842 1493
Size: 1862 226 152 2109 1697
Fragments arranged by size:
2109 1862 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4600 160
Size: 872 3568 1606
Fragments arranged by size:
3568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 2286 3371 1870
Size: 73 343 1085 4545
Fragments arranged by size:
4545 1085 343 73
BamHI G'GATC_C
Cuts at: 3376 3743 3376
Size: 367 5679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2877 3367 4822 618
Size: 325 217 627 1090 490 1455 1842
Fragments arranged by size:
1842 1455 1090 627 490 325 217
BanII G_rGCy'C
Cuts at: 1391 2149 2332 3365 1391
Size: 758 183 1033 4072
Fragments arranged by size:
4072 1033 758 183
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5075
BbvCI CC'TCA_GC
Cuts at: 2046 2046
Size: 6046
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4237 4271 1903
Size: 34 50 34 2216 34 3678
Fragments arranged by size:
3678 2216 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3204 3938 5148 3204
Size: 734 1210 4102
Fragments arranged by size:
4102 1210 734
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4657 136
Size: 107 122 71 631 605 2985 1525
Fragments arranged by size:
2985 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2116 2476 6041 2116
Size: 360 3565 2121
Fragments arranged by size:
3565 2121 360
BlpI GC'TnA_GC
Cuts at: 3463 3463
Size: 6046
Bme1580I G_kGCm'C
Cuts at: 1790 4107 5263 1790
Size: 2317 1156 2573
Fragments arranged by size:
2573 2317 1156
BmgBI CAC'GTC
Cuts at: 1318 2760 1318
Size: 1442 4604
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3020 3777 4735
Size: 416 53 83 399 24 1603 757 958
Cuts at: 4735 442
Size: 1753
Fragments arranged by size:
1753 1603 958 757 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2243 2243
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2154 2213 2397 2785 4706 1239
Size: 878 37 59 184 388 1921 2579
Fragments arranged by size:
2579 1921 878 388 184 59 37
Bpu10I CC'TnA_GC
Cuts at: 2046 2541 2046
Size: 495 5551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 4171 5036 5334 2567
Size: 1604 865 298 3279
Fragments arranged by size:
3279 1604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2415 2682 4709 882
Size: 617 916 267 2027 2219
Fragments arranged by size:
2219 2027 916 617 267
BsaAI yAC'GTr
Cuts at: 493 1204 3837 493
Size: 711 2633 2702
Fragments arranged by size:
2702 2633 711
BsaBI GATnn'nnATC
Cuts at: 2597 6040 2597
Size: 3443 2603
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2704 3853 4235 4870
Size: 53 83 186 936 1171 1149 382 635
Cuts at: 4870 275
Size: 1451
Fragments arranged by size:
1451 1171 1149 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4479 4877 5151 5298 5709 4479
Size: 398 274 147 411 4816
Fragments arranged by size:
4816 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2395 2425 2636 2666 1178
Size: 30 165 30 992 30 211 30 4558
Fragments arranged by size:
4558 992 211 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6046
BseYI C'CCAG_C
Cuts at: 5249 5745 5249
Size: 496 5550
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2683 1163
Size: 573 947 4526
Fragments arranged by size:
4526 947 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3496 4257 5285 1540
Size: 275 186 1495 761 1028 2301
Fragments arranged by size:
2301 1495 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2149 2332 4107 4192 5263 2149
Size: 183 1775 85 1071 2932
Fragments arranged by size:
2932 1775 1071 183 85
BsmI GAATG_Cn'
Cuts at: 3557 3650 3557
Size: 93 5953
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2149 2332 3365 4107 4192 5263 1391
Size: 399 359 183 1033 742 85 1071 2174
Fragments arranged by size:
2174 1071 1033 742 399 359 183 85
BspEI T'CCGG_A
Cuts at: 5709 5709
Size: 6046
BspHI T'CATG_A
Cuts at: 3935 5665 3935
Size: 1730 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3401 844
Size: 2557 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3933 1394
Size: 429 2110 3507
Fragments arranged by size:
3507 2110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4541 4715 65
Size: 1183 3293 174 1396
Fragments arranged by size:
3293 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4690 4877 1700
Size: 110 2880 187 2869
Fragments arranged by size:
2880 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2595 3130 3285 96
Size: 2499 535 155 2857
Fragments arranged by size:
2857 2499 535 155
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6046
BssSI C'ACGA_G
Cuts at: 2790 4106 5118 2790
Size: 1316 1012 3718
Fragments arranged by size:
3718 1316 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3263 794
Size: 2469 3577
BstBI TT'CG_AA
Cuts at: 4850 4850
Size: 6046
BstEII G'GTnAC_C
Cuts at: 1184 3182 1184
Size: 1998 4048
BstYI r'GATC_y
Cuts at: 1972 2116 2476 3376 3743 4128 4145 5597 5683
Size: 144 360 900 367 385 17 1452 86
Cuts at: 5683 5695 6041 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 900 385 367 360 346 144
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2034 2034
Size: 6046
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5307
Fragments arranged by size:
5307 683 56
BtsI GCAGTG_nn'
Cuts at: 3562 4356 4376 3562
Size: 794 20 5232
Fragments arranged by size:
5232 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DraIII CAC_nnn'GTG
Cuts at: 2884 2884
Size: 6046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5053 809
Size: 507 3737 1802
Fragments arranged by size:
3737 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2920 3493 4382
Size: 54 1425 50 275 18 1090 573 889
Cuts at: 4382 6011 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1090 889 573 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3493 1537
Size: 275 1681 4090
Fragments arranged by size:
4090 1681 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2300 2943 3976 1353
Size: 947 643 1033 3423
Fragments arranged by size:
3423 1033 947 643
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4633 5007 5153 387
Size: 1064 138 3044 374 146 1280
Fragments arranged by size:
3044 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2147 2330 2147
Size: 183 5863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2154 2213 2397 2785 2967 4109
Size: 393 485 37 59 184 388 182 1142
Cuts at: 4109 4706 5493 5761 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1142 787 597 485 393 388 268
184 182 59 37
EcoO109I rG'GnC_Cy
Cuts at: 2587 2587
Size: 6046
EcoRI G'AATT_C
Cuts at: 3355 3733 3355
Size: 378 5668
FspI TGC'GCA
Cuts at: 4552 4552
Size: 6046
HaeII r_GCGC'y
Cuts at: 1678 5193 1678
Size: 3515 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 2621 2636 2653
Size: 32 135 32 896 32 121 15 17
Cuts at: 2653 2661 2668 2693 2942 2974 4687 4719 4761
Size: 8 7 25 249 32 1713 32 42
Cuts at: 4761 4793 1373
Size: 32 2626
Fragments arranged by size:
2626 1713 896 249 135 121 42 32
32 32 32 32 32 25 17 15
8 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2937 3390 3636 669
Size: 988 281 999 453 246 3079
Fragments arranged by size:
3079 999 988 453 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2410 5160 5344 1305
Size: 1105 2750 184 2007
Fragments arranged by size:
2750 2007 1105 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2922 10
Size: 54 1425 343 1090 3134
Fragments arranged by size:
3134 1425 1090 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 4004 4363 4522 518
Size: 648 1404 1434 359 159 2042
Fragments arranged by size:
2042 1434 1404 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3462 4139 5287 5532 1606
Size: 1856 677 1148 245 2120
Fragments arranged by size:
2120 1856 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5494
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 3493 3493
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 3404 4949 1132
Size: 97 2175 1545 2229
Fragments arranged by size:
2229 2175 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6046
PciI A'CATG_T
Cuts at: 4945 4945
Size: 6046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6046
PfoI T'CCnGG_A
Cuts at: 2762 2762
Size: 6046
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2587 2587
Size: 6046
PshAI GACnn'nnGTC
Cuts at: 1613 3257 1613
Size: 1644 4402
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2971 3003 2971
Size: 32 6014
PstI C_TGCA'G
Cuts at: 830 2124 3259 3398 830
Size: 1294 1135 139 3478
Fragments arranged by size:
3478 1294 1135 139
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6046
SacI G_AGCT'C
Cuts at: 2149 2332 2149
Size: 183 5863
SalI G'TCGA_C
Cuts at: 1936 3388 1936
Size: 1452 4594
SapI GCTCTTCn'nnn_
Cuts at: 2943 2943
Size: 6046
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SfcI C'TryA_G
Cuts at: 826 1045 2120 2866 3164 3255 3394 4529 5210
Size: 219 1075 746 298 91 139 1135 681
Cuts at: 5210 5401 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1075 746 681 298 219 191
139 91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 3373 1872
Size: 1501 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2286 2546 4150 5051 5313 820
Size: 197 926 343 260 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 343 262 260 197
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 3404 1132
Size: 2272 3774
SspI AAT'ATT
Cuts at: 5 52 2891 3970 5
Size: 47 2839 1079 2081
Fragments arranged by size:
2839 2081 1079 47
StuI AGG'CCT
Cuts at: 1517 3340 1517
Size: 1823 4223
StyI C'CwwG_G
Cuts at: 513 1065 3485 513
Size: 552 2420 3074
Fragments arranged by size:
3074 2420 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2599 4073 4090 4243 4428 4873 325
Size: 1255 1019 1474 17 153 185 445 1498
Fragments arranged by size:
1498 1474 1255 1019 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 3130 3285
Size: 274 80 33 51 1443 618 535 155
Cuts at: 3285 4292 6004 96
Size: 1007 1712 138
Fragments arranged by size:
1712 1443 1007 618 535 274 155 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6006 6038 6006
Size: 32 6014
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6046
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XhoI C'TCGA_G
Cuts at: 1943 2286 1943
Size: 343 5703
XmaI C'CCGG_G
Cuts at: 1870 3371 1870
Size: 1501 4545
XmnI GAAnn'nnTTC
Cuts at: 4175 4175
Size: 6046
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3854 276
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PvuII RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI