Restriction map for
pFN21AE3190
(Circular) (Six-base) MAPSORT of: pFN21AE3190.seq Check: 6169 from: 1 to: 6031
pFN21AE3190 6031 bp
With 182 enzymes: SgfI *
July 14, 2018 22:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2064 2064
Size: 6031
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3841 278
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3374 1937
Size: 1437 4594
Acc65I G'GTAC_C
Cuts at: 3352 3352
Size: 6031
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 3312 4094 5478 5746 1632
Size: 653 1027 782 1384 268 1917
Fragments arranged by size:
1917 1384 1027 782 653 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5834
AflIII A'CryG_T
Cuts at: 1203 3228 4930 5907 1203
Size: 2025 1702 977 1327
Fragments arranged by size:
2025 1702 1327 977
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 4760 4760
Size: 6031
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6031
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2049 2072 2234 1184
Size: 865 23 162 4981
Fragments arranged by size:
4981 865 162 23
AlwNI CAG_nnn'CTG
Cuts at: 3290 3774 5346 3290
Size: 484 1572 3975
Fragments arranged by size:
3975 1572 484
ApaLI G'TGCA_C
Cuts at: 4088 5244 4088
Size: 1156 4875
ApoI r'AATT_y
Cuts at: 1493 2146 2201 2338 2553 2889 3242 3340 3566
Size: 653 55 137 215 336 353 98 226
Cuts at: 3566 3718 5827 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 653 353 336 226 215 152
137 98 55
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 3356 1870
Size: 73 1413 4545
Fragments arranged by size:
4545 1413 73
AvrII C'CTAG_G
Cuts at: 2182 2182
Size: 6031
BamHI G'GATC_C
Cuts at: 3361 3728 3361
Size: 367 5664
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3352 4807 618
Size: 325 217 627 1565 1455 1842
Fragments arranged by size:
1842 1565 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3350 1391
Size: 1959 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2112 2494 2658 2858 928
Size: 971 213 382 164 200 4101
Fragments arranged by size:
4101 971 382 213 200 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4222 4256 1903
Size: 34 2285 34 3678
Fragments arranged by size:
3678 2285 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3923 5133 3923
Size: 1210 4821
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2731 4642 136
Size: 107 122 71 631 605 1059 1911 1525
Fragments arranged by size:
1911 1525 1059 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3180 6026 3180
Size: 2846 3185
BlpI GC'TnA_GC
Cuts at: 3448 3448
Size: 6031
Bme1580I G_kGCm'C
Cuts at: 1790 4092 5248 1790
Size: 2302 1156 2573
Fragments arranged by size:
2573 2302 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6031
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3166 3762 4720
Size: 416 53 83 399 24 1749 596 958
Cuts at: 4720 442
Size: 1753
Fragments arranged by size:
1753 1749 958 596 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4691 1239
Size: 3452 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2301 2528 2629 4156 5021 5319 2301
Size: 227 101 1527 865 298 3013
Fragments arranged by size:
3013 1527 865 298 227 101
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2750 4694 882
Size: 617 1251 1944 2219
Fragments arranged by size:
2219 1944 1251 617
BsaAI yAC'GTr
Cuts at: 493 1204 2789 3822 493
Size: 711 1585 1033 2702
Fragments arranged by size:
2702 1585 1033 711
BsaBI GATnn'nnATC
Cuts at: 2270 2802 6025 2270
Size: 532 3223 2276
Fragments arranged by size:
3223 2276 532
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3838 4220 4855 275
Size: 53 83 186 936 2305 382 635 1451
Fragments arranged by size:
2305 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4464 4862 5136 5283 5694 4464
Size: 398 274 147 411 4801
Fragments arranged by size:
4801 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5806
Fragments arranged by size:
5806 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6031
BseYI C'CCAG_C
Cuts at: 5234 5730 5234
Size: 496 5535
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5458
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3481 4242 5270 1540
Size: 275 186 1480 761 1028 2301
Fragments arranged by size:
2301 1480 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4092 4177 5248 4092
Size: 85 1071 4875
Fragments arranged by size:
4875 1071 85
BsmI GAATG_Cn'
Cuts at: 3542 3635 3542
Size: 93 5938
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3350 4092 4177 5248 1391
Size: 399 1560 742 85 1071 2174
Fragments arranged by size:
2174 1560 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5694 5694
Size: 6031
BspHI T'CATG_A
Cuts at: 3920 5650 3920
Size: 1730 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2064 3386 844
Size: 1220 1322 3489
Fragments arranged by size:
3489 1322 1220
BsrBI CCG'CTC
Cuts at: 1394 1823 2062 3918 1394
Size: 429 239 1856 3507
Fragments arranged by size:
3507 1856 429 239
BsrDI GCAATG_nn'
Cuts at: 65 1248 2389 4526 4700 65
Size: 1183 1141 2137 174 1396
Fragments arranged by size:
2137 1396 1183 1141 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 3120 4675 4862 1700
Size: 110 199 1111 1555 187 2869
Fragments arranged by size:
2869 1555 1111 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 2386 96
Size: 2290 3741
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6031
BssSI C'ACGA_G
Cuts at: 4091 5103 4091
Size: 1012 5019
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6031
BstBI TT'CG_AA
Cuts at: 4835 4835
Size: 6031
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6031
BstYI r'GATC_y
Cuts at: 1972 3180 3361 3728 4113 4130 5582 5668 5680
Size: 1208 181 367 385 17 1452 86 12
Cuts at: 5680 6026 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1208 385 367 346 181 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4540
Fragments arranged by size:
4540 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5292
Fragments arranged by size:
5292 683 56
BtsI GCAGTG_nn'
Cuts at: 2917 3547 4341 4361 2917
Size: 630 794 20 4587
Fragments arranged by size:
4587 794 630 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5996
DraI TTT'AAA
Cuts at: 3335 3335
Size: 6031
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5038 809
Size: 507 3722 1802
Fragments arranged by size:
3722 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3213 3478
Size: 54 1425 50 275 18 177 1206 265
Cuts at: 3478 4367 5996 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1206 889 275 265 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3478 1537
Size: 275 1666 4090
Fragments arranged by size:
4090 1666 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2304 2684 2826 3961 1353
Size: 951 380 142 1135 3423
Fragments arranged by size:
3423 1135 951 380 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4618 4992 5138 387
Size: 1064 138 3029 374 146 1280
Fragments arranged by size:
3029 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 3312 4094 4691 5478 5746 1239
Size: 393 653 1027 782 597 787 268 1524
Fragments arranged by size:
1524 1027 787 782 653 597 393 268
EcoRI G'AATT_C
Cuts at: 2889 3340 3718 2889
Size: 451 378 5202
Fragments arranged by size:
5202 451 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3338 3338
Size: 6031
FseI GG_CCGG'CC
Cuts at: 3124 3124
Size: 6031
FspI TGC'GCA
Cuts at: 2037 4537 2037
Size: 2500 3531
HaeII r_GCGC'y
Cuts at: 1678 5178 1678
Size: 3500 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2281 2313 4672 4704 4746
Size: 32 135 32 709 32 2359 32 42
Cuts at: 4746 4778 1373
Size: 32 2626
Fragments arranged by size:
2626 2359 709 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3375 3621 669
Size: 988 281 1437 246 3079
Fragments arranged by size:
3079 1437 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6031
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KpnI G_GTAC'C
Cuts at: 3356 3356
Size: 6031
MfeI C'AATT_G
Cuts at: 2481 3630 2481
Size: 1149 4882
MluI A'CGCG_T
Cuts at: 5907 5907
Size: 6031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5145 5329 1305
Size: 3840 184 2007
Fragments arranged by size:
3840 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4209
Fragments arranged by size:
4209 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2668 3066 3989 4348 4507 518
Size: 648 1502 398 923 359 159 2042
Fragments arranged by size:
2042 1502 923 648 398 359 159
MspA1I CmG'CkG
Cuts at: 1606 2235 3141 3447 4124 5272 5517 1606
Size: 629 906 306 677 1148 245 2120
Fragments arranged by size:
2120 1148 906 677 629 306 245
NaeI GCC'GGC
Cuts at: 2011 3122 2011
Size: 1111 4920
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4540
Fragments arranged by size:
4540 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6031
NgoMIV G'CCGG_C
Cuts at: 2009 3120 2009
Size: 1111 4920
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6031
NotI GC'GGCC_GC
Cuts at: 3478 3478
Size: 6031
NspI r_CATG'y
Cuts at: 1132 1229 3232 3389 4934 1132
Size: 97 2003 157 1545 2229
Fragments arranged by size:
2229 2003 1545 157 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6031
PciI A'CATG_T
Cuts at: 3228 4930 3228
Size: 1702 4329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6031
PfoI T'CCnGG_A
Cuts at: 2281 2281
Size: 6031
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 5613 5645 4100
Size: 32 1481 32 4486
Fragments arranged by size:
4486 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2157 2235 1613
Size: 544 78 5409
Fragments arranged by size:
5409 544 78
PsiI TTA'TAA
Cuts at: 3601 3601
Size: 6031
PstI C_TGCA'G
Cuts at: 830 2074 2593 3383 830
Size: 1244 519 790 3478
Fragments arranged by size:
3478 1244 790 519
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6031
PvuII CAG'CTG
Cuts at: 2235 3141 2235
Size: 906 5125
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SbfI CC_TGCA'GG
Cuts at: 3383 3383
Size: 6031
ScaI AGT'ACT
Cuts at: 4279 5991 4279
Size: 1712 4319
SfcI C'TryA_G
Cuts at: 826 1045 2070 2589 3129 3379 4514 5195 5386
Size: 219 1025 519 540 250 1135 681 191
Cuts at: 5386 826
Size: 1471
Fragments arranged by size:
1471 1135 1025 681 540 519 250 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6031
SmaI CCC'GGG
Cuts at: 1872 3358 1872
Size: 1486 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2316 2507 2644 4135 5036 5298
Size: 197 926 373 191 137 1491 901 262
Cuts at: 5298 820
Size: 1553
Fragments arranged by size:
1553 1491 926 901 373 262 197 191
137
SnaBI TAC'GTA
Cuts at: 493 3822 493
Size: 3329 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6031
SphI G_CATG'C
Cuts at: 1132 3389 1132
Size: 2257 3774
SspI AAT'ATT
Cuts at: 5 52 3015 3955 5
Size: 47 2963 940 2081
Fragments arranged by size:
2963 2081 940 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6031
StyI C'CwwG_G
Cuts at: 513 1065 2004 2182 3470 513
Size: 552 939 178 1288 3074
Fragments arranged by size:
3074 1288 939 552 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4058 4075 4228 4413 4858 325
Size: 1255 2478 17 153 185 445 1498
Fragments arranged by size:
2478 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2386 2957 4277
Size: 274 80 33 51 1443 409 571 1320
Cuts at: 4277 5989 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1320 571 409 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5991 6023 5991
Size: 32 5999
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6031
XbaI T'CTAG_A
Cuts at: 3367 3367
Size: 6031
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 3356 1870
Size: 1486 4545
XmnI GAAnn'nnTTC
Cuts at: 2202 4160 2202
Size: 1958 4073
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3839 276
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI