Restriction map for
pFN21AE3188
(Circular) (Six-base) MAPSORT of: pFN21AE3188.seq Check: 3626 from: 1 to: 6001
pFN21AE3188 6001 bp
With 182 enzymes: SgfI *
March 28, 2012 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2226 2226
Size: 6001
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2442 3344 1937
Size: 505 902 4594
Fragments arranged by size:
4594 902 505
Acc65I G'GTAC_C
Cuts at: 3322 3322
Size: 6001
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2688 4064 5448 5716 1632
Size: 1056 1376 1384 268 1917
Fragments arranged by size:
1917 1384 1376 1056 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 4900 5877 1203
Size: 3697 977 1327
Fragments arranged by size:
3697 1327 977
AgeI A'CCGG_T
Cuts at: 4832 4832
Size: 6001
AhdI GACnn_n'nnGTC
Cuts at: 4730 4730
Size: 6001
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6001
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2493 2525 2493
Size: 32 5969
AlwNI CAG_nnn'CTG
Cuts at: 2083 2124 2245 2416 2486 2849 2926 2976 3022
Size: 41 121 171 70 363 77 50 46
Cuts at: 3022 3744 5316 2083
Size: 722 1572 2768
Fragments arranged by size:
2768 1572 722 363 171 121 77 70
50 46 41
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 2622 3310 3536 3688 5797 1493
Size: 1129 688 226 152 2109 1697
Fragments arranged by size:
2109 1697 1129 688 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 2314 3326 1870
Size: 73 371 1012 4545
Fragments arranged by size:
4545 1012 371 73
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2153 2198 2355 3322 4777
Size: 325 217 627 366 45 157 967 1455
Cuts at: 4777 618
Size: 1842
Fragments arranged by size:
1842 1455 967 627 366 325 217 157
45
BanII G_rGCy'C
Cuts at: 1391 2027 2291 2321 3320 1391
Size: 636 264 30 999 4072
Fragments arranged by size:
4072 999 636 264 30
BbeI G_GCGC'C
Cuts at: 2157 2202 2359 2157
Size: 45 157 5799
Fragments arranged by size:
5799 157 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2697 2922 928
Size: 971 798 225 4007
Fragments arranged by size:
4007 971 798 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2564 2598 4192 4226 1903
Size: 34 627 34 1594 34 3678
Fragments arranged by size:
3678 1594 627 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3893 5103 3893
Size: 1210 4791
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2196 4612 136
Size: 107 122 71 631 605 524 2416 1525
Fragments arranged by size:
2416 1525 631 605 524 122 107 71
BglII A'GATC_T
Cuts at: 5996 5996
Size: 6001
BlpI GC'TnA_GC
Cuts at: 3418 3418
Size: 6001
Bme1580I G_kGCm'C
Cuts at: 1790 4062 5218 1790
Size: 2272 1156 2573
Fragments arranged by size:
2573 2272 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2956 2986 3732
Size: 416 53 83 399 24 1539 30 746
Cuts at: 3732 4690 442
Size: 958 1753
Fragments arranged by size:
1753 1539 958 746 416 399 83 53
30 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2621 2775 2621
Size: 154 5847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2305 2317 2840 2899 4661 1239
Size: 920 146 12 523 59 1762 2579
Fragments arranged by size:
2579 1762 920 523 146 59 12
Bpu10I CC'TnA_GC
Cuts at: 2409 2409
Size: 6001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3170 4126 4991 5289 3170
Size: 956 865 298 3882
Fragments arranged by size:
3882 956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4664 882
Size: 617 3165 2219
Fragments arranged by size:
3165 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3792 493
Size: 711 2588 2702
Fragments arranged by size:
2702 2588 711
BsaBI GATnn'nnATC
Cuts at: 5995 5995
Size: 6001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2154 2163 2199 2356
Size: 53 83 186 936 621 9 36 157
Cuts at: 2356 3808 4190 4825 275
Size: 1452 382 635 1451
Fragments arranged by size:
1452 1451 936 635 621 382 186 157
83 53 36 9
BsaWI w'CCGG_w
Cuts at: 4434 4832 5106 5253 5664 4434
Size: 398 274 147 411 4771
Fragments arranged by size:
4771 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2132 2162 1178
Size: 30 165 30 729 30 5017
Fragments arranged by size:
5017 729 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2701 3167 3214 1186
Size: 1515 466 47 3973
Fragments arranged by size:
3973 1515 466 47
BseYI C'CCAG_C
Cuts at: 2172 5204 5700 2172
Size: 3032 496 2473
Fragments arranged by size:
3032 2473 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2727 1163
Size: 573 991 4437
Fragments arranged by size:
4437 991 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2343 3451 4212 5240 1540
Size: 275 186 342 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 342 275 186
BsiHKAI G_wGCw'C
Cuts at: 4062 4147 5218 4062
Size: 85 1071 4845
Fragments arranged by size:
4845 1071 85
BsmI GAATG_Cn'
Cuts at: 3512 3605 3512
Size: 93 5908
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2291 2321 3320 4062 4147 5218
Size: 399 237 264 30 999 742 85 1071
Cuts at: 5218 1391
Size: 2174
Fragments arranged by size:
2174 1071 999 742 399 264 237 85
30
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 3890 5620 3890
Size: 1730 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2226 3356 844
Size: 1382 1130 3489
Fragments arranged by size:
3489 1382 1130
BsrBI CCG'CTC
Cuts at: 1394 1823 3888 1394
Size: 429 2065 3507
Fragments arranged by size:
3507 2065 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2720 4496 4670 65
Size: 1183 1472 1776 174 1396
Fragments arranged by size:
1776 1472 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4645 4832 1700
Size: 110 2835 187 2869
Fragments arranged by size:
2869 2835 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6001
BssSI C'ACGA_G
Cuts at: 4061 5073 4061
Size: 1012 4989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2073 794
Size: 1279 4722
BstBI TT'CG_AA
Cuts at: 4805 4805
Size: 6001
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6001
BstYI r'GATC_y
Cuts at: 1972 2539 3331 3698 4083 4100 5552 5638 5650
Size: 567 792 367 385 17 1452 86 12
Cuts at: 5650 5996 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 792 567 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2547 513
Size: 552 939 543 3967
Fragments arranged by size:
3967 939 552 543
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5262
Fragments arranged by size:
5262 683 56
BtsI GCAGTG_nn'
Cuts at: 2131 2302 3517 4311 4331 2131
Size: 171 1215 794 20 3801
Fragments arranged by size:
3801 1215 794 171 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5008 809
Size: 507 3692 1802
Fragments arranged by size:
3692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2113 2247
Size: 54 1425 50 275 18 216 67 134
Cuts at: 2247 2340 3448 4337 5966 8
Size: 93 1108 889 1629 43
Fragments arranged by size:
1629 1425 1108 889 275 216 134 93
67 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2340 3448 1537
Size: 275 528 1108 4090
Fragments arranged by size:
4090 1108 528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2384 2795 3931 1353
Size: 1031 411 1136 3423
Fragments arranged by size:
3423 1136 1031 411
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2017 4588 4962 5108 387
Size: 1064 138 428 2571 374 146 1280
Fragments arranged by size:
2571 1280 1064 428 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2305 2317 2688 2840 2899 4064
Size: 393 527 146 12 371 152 59 1165
Cuts at: 4064 4661 5448 5716 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1165 787 597 527 393 371 268
152 146 59 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2516 3152 2516
Size: 636 5365
EcoO109I rG'GnC_Cy
Cuts at: 2143 2242 2349 2472 2923 3019 2143
Size: 99 107 123 451 96 5125
Fragments arranged by size:
5125 451 123 107 99 96
EcoRI G'AATT_C
Cuts at: 3310 3688 3310
Size: 378 5623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3184 3184
Size: 6001
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 2157 2202 2359 5148 1678
Size: 479 45 157 2789 2531
Fragments arranged by size:
2789 2531 479 157 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2839 2871 4642 4674 4716
Size: 32 135 32 1267 32 1771 32 42
Cuts at: 4716 4748 1373
Size: 32 2626
Fragments arranged by size:
2626 1771 1267 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3591 669
Size: 988 281 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3161 3194 748
Size: 2413 33 3555
Fragments arranged by size:
3555 2413 33
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KasI G'GCGC_C
Cuts at: 2153 2198 2355 2153
Size: 45 157 5799
Fragments arranged by size:
5799 157 45
KpnI G_GTAC'C
Cuts at: 3326 3326
Size: 6001
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2316 2598 2810 5115 5299 1305
Size: 1011 282 212 2305 184 2007
Fragments arranged by size:
2305 2007 1011 282 212 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4179
Fragments arranged by size:
4179 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3959 4318 4477 518
Size: 648 2793 359 159 2042
Fragments arranged by size:
2793 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2172 2374 2582 3417 4094 5242 5487 1606
Size: 566 202 208 835 677 1148 245 2120
Fragments arranged by size:
2120 1148 835 677 566 245 208 202
NarI GG'CG_CC
Cuts at: 2154 2199 2356 2154
Size: 45 157 5799
Fragments arranged by size:
5799 157 45
NcoI C'CATG_G
Cuts at: 513 1065 2004 2547 513
Size: 552 939 543 3967
Fragments arranged by size:
3967 939 552 543
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 2340 3448 2340
Size: 1108 4893
NspI r_CATG'y
Cuts at: 1132 1229 3359 4904 1132
Size: 97 2130 1545 2229
Fragments arranged by size:
2229 2130 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6001
PciI A'CATG_T
Cuts at: 4900 4900
Size: 6001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2137 3022 1108
Size: 1029 885 4087
Fragments arranged by size:
4087 1029 885
PfoI T'CCnGG_A
Cuts at: 2691 3265 2691
Size: 574 5427
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 5583 5615 4070
Size: 32 1481 32 4456
Fragments arranged by size:
4456 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2242 3019 2242
Size: 777 5224
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PstI C_TGCA'G
Cuts at: 830 2241 2373 2463 2587 2816 3353 830
Size: 1411 132 90 124 229 537 3478
Fragments arranged by size:
3478 1411 537 229 132 124 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 2582 2582
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SapI GCTCTTCn'nnn_
Cuts at: 2384 2795 2384
Size: 411 5590
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 4249 5961 4249
Size: 1712 4289
SexAI A'CCwGG_T
Cuts at: 3015 3015
Size: 6001
SfcI C'TryA_G
Cuts at: 826 1045 2237 2369 2459 2583 2812 3349 4484
Size: 219 1192 132 90 124 229 537 1135
Cuts at: 4484 5165 5356 826
Size: 681 191 1471
Fragments arranged by size:
1471 1192 1135 681 537 229 219 191
132 124 90
SfoI GGC'GCC
Cuts at: 2155 2200 2357 2155
Size: 45 157 5799
Fragments arranged by size:
5799 157 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 2316 3328 1872
Size: 444 1012 4545
Fragments arranged by size:
4545 1012 444
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3149 4105 5006 5268 820
Size: 197 926 1206 956 901 262 1553
Fragments arranged by size:
1553 1206 956 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 3359 1132
Size: 2227 3774
SrfI GCCC'GGGC
Cuts at: 2316 2316
Size: 6001
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6001
StyI C'CwwG_G
Cuts at: 513 1065 2004 2547 2829 3106 3440 513
Size: 552 939 543 282 277 334 3074
Fragments arranged by size:
3074 939 552 543 334 282 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4028 4045 4198 4383 4828 325
Size: 1255 2448 17 153 185 445 1498
Fragments arranged by size:
2448 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4247 5959 96
Size: 274 80 33 51 1443 2270 1712 138
Fragments arranged by size:
2270 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2132 2164 5961 5993 2132
Size: 32 3797 32 2140
Fragments arranged by size:
3797 2140 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2447 2851 3017 1316
Size: 1131 404 166 4300
Fragments arranged by size:
4300 1131 404 166
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2981 2011
Size: 970 5031
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 2314 3326 1870
Size: 444 1012 4545
Fragments arranged by size:
4545 1012 444
XmnI GAAnn'nnTTC
Cuts at: 2622 4130 2622
Size: 1508 4493
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SfiI SwaI