Restriction map for
pFN21AE3185
(Circular) (Six-base) MAPSORT of: pFN21AE3185.seq Check: 6574 from: 1 to: 5980
pFN21AE3185 5980 bp
With 182 enzymes: SgfI *
March 28, 2012 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2387 3323 1937
Size: 450 936 4594
Fragments arranged by size:
4594 936 450
Acc65I G'GTAC_C
Cuts at: 2486 3301 2486
Size: 815 5165
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3296 4043 5427 5695 1632
Size: 1664 747 1384 268 1917
Fragments arranged by size:
1917 1664 1384 747 268
AfeI AGC'GCT
Cuts at: 2867 2867
Size: 5980
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 2243 2375 2444 1166
Size: 1077 132 69 4702
Fragments arranged by size:
4702 1077 132 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2852 1184
Size: 1668 4312
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 5948
AlwNI CAG_nnn'CTG
Cuts at: 2728 3723 5295 2728
Size: 995 1572 3413
Fragments arranged by size:
3413 1572 995
ApaI G_GGCC'C
Cuts at: 2369 2500 2369
Size: 131 5849
ApaLI G'TGCA_C
Cuts at: 3089 4037 5193 3089
Size: 948 1156 3876
Fragments arranged by size:
3876 1156 948
ApoI r'AATT_y
Cuts at: 1493 2045 2165 2963 3289 3515 3667 5776 1493
Size: 552 120 798 326 226 152 2109 1697
Fragments arranged by size:
2109 1697 798 552 326 226 152 120
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2349 3305 1870
Size: 73 406 956 4545
Fragments arranged by size:
4545 956 406 73
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2183 2486 2995 3301 4756
Size: 325 217 627 396 303 509 306 1455
Cuts at: 4756 618
Size: 1842
Fragments arranged by size:
1842 1455 627 509 396 325 306 303
217
BanII G_rGCy'C
Cuts at: 1391 2369 2500 2714 3299 1391
Size: 978 131 214 585 4072
Fragments arranged by size:
4072 978 585 214 131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5009
BbvCI CC'TCA_GC
Cuts at: 2743 2743
Size: 5980
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2498 2532 4171 4205 1903
Size: 34 561 34 1639 34 3678
Fragments arranged by size:
3678 1639 561 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2915 3872 5082 2915
Size: 957 1210 3813
Fragments arranged by size:
3813 1210 957
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2192 4591 136
Size: 107 122 71 631 605 520 2399 1525
Fragments arranged by size:
2399 1525 631 605 520 122 107 71
BglII A'GATC_T
Cuts at: 2133 5975 2133
Size: 3842 2138
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 2369 2500 2998 3093 4041 5197 1790
Size: 579 131 498 95 948 1156 2573
Fragments arranged by size:
2573 1156 948 579 498 131 95
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3198 3206 4640 1239
Size: 1959 8 1434 2579
Fragments arranged by size:
2579 1959 1434 8
Bpu10I CC'TnA_GC
Cuts at: 2743 2743
Size: 5980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3021 3038 4105 4970 5268 3021
Size: 17 1067 865 298 3733
Fragments arranged by size:
3733 1067 865 298 17
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4643 882
Size: 617 3144 2219
Fragments arranged by size:
3144 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3261 4413 4811 5085 5232 5643 3261
Size: 1152 398 274 147 411 3598
Fragments arranged by size:
3598 1152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2796 2826 3122 3152 1178
Size: 30 165 30 1393 30 296 30 4006
Fragments arranged by size:
4006 1393 296 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3143 1186
Size: 1957 4023
BseYI C'CCAG_C
Cuts at: 2700 5183 5679 2700
Size: 2483 496 3001
Fragments arranged by size:
3001 2483 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5407
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2236 3430 4191 5219 1540
Size: 275 186 235 1194 761 1028 2301
Fragments arranged by size:
2301 1194 1028 761 275 235 186
BsiHKAI G_wGCw'C
Cuts at: 3093 4041 4126 5197 3093
Size: 948 85 1071 3876
Fragments arranged by size:
3876 1071 948 85
BsmI GAATG_Cn'
Cuts at: 3491 3584 3491
Size: 93 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2369 2500 2714 2998 3093 3299 4041
Size: 399 579 131 214 284 95 206 742
Cuts at: 4041 4126 5197 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 579 399 284 214 206
131 95 85
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3335 844
Size: 2491 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2232 2512 3867 1394
Size: 429 409 280 1355 3507
Fragments arranged by size:
3507 1355 429 409 280
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4624 4811 1700
Size: 110 2814 187 2869
Fragments arranged by size:
2869 2814 187 110
BsrGI T'GTAC_A
Cuts at: 96 2790 96
Size: 2694 3286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5980
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 2717 3169 1184
Size: 1533 452 3995
Fragments arranged by size:
3995 1533 452
BstXI CCAn_nnnn'nTGG
Cuts at: 2377 2446 2377
Size: 69 5911
BstYI r'GATC_y
Cuts at: 1972 2133 3271 3310 3677 4062 4079 5531 5617
Size: 161 1138 39 367 385 17 1452 86
Cuts at: 5617 5629 5975 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1138 385 367 346 161 86
39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2490 513
Size: 552 939 486 4003
Fragments arranged by size:
4003 939 552 486
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 2424 3111 3227 3496 4290 4310 2424
Size: 687 116 269 794 20 4094
Fragments arranged by size:
4094 794 687 269 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2029 2064 529
Size: 35 1465 35 4445
Fragments arranged by size:
4445 1465 35 35
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 3427 4316
Size: 54 1425 50 275 18 403 1194 889
Cuts at: 4316 5945 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1194 889 403 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2233 3427 1537
Size: 275 421 1194 4090
Fragments arranged by size:
4090 1194 421 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2007 2331 2523 3910 1353
Size: 654 324 192 1387 3423
Fragments arranged by size:
3423 1387 654 324 192
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4567 4941 5087 387
Size: 1064 138 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3198 3206 3296 4043 4640 5427 5695
Size: 393 1566 8 90 747 597 787 268
Cuts at: 5695 1239
Size: 1524
Fragments arranged by size:
1566 1524 787 747 597 393 268 90
8
EcoO109I rG'GnC_Cy
Cuts at: 2345 2366 2496 2497 2548 2809 2345
Size: 21 130 1 51 261 5516
Fragments arranged by size:
5516 261 130 51 21 1
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
EcoRV GAT'ATC
Cuts at: 3259 3259
Size: 5980
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2869 5127 1678
Size: 1191 2258 2531
Fragments arranged by size:
2531 2258 1191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2602 2634 3122 3154 4621
Size: 32 135 32 1030 32 488 32 1467
Cuts at: 4621 4653 4695 4727 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1467 1030 488 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2118 3324 3570 669
Size: 988 281 180 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246 180
HindIII A'AGCT_T
Cuts at: 748 2597 748
Size: 1849 4131
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 2490 3305 2490
Size: 815 5165
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2088 5094 5278 1305
Size: 783 3006 184 2007
Fragments arranged by size:
3006 2007 783 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4158
Fragments arranged by size:
4158 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 2270 2375 2444 3167 3938 4297
Size: 648 1077 27 105 69 723 771 359
Cuts at: 4297 4456 518
Size: 159 2042
Fragments arranged by size:
2042 1077 771 723 648 359 159 105
69 27
MspA1I CmG'CkG
Cuts at: 1606 3396 4073 5221 5466 1606
Size: 1790 677 1148 245 2120
Fragments arranged by size:
2120 1790 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2490 513
Size: 552 939 486 4003
Fragments arranged by size:
4003 939 552 486
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 2233 3427 2233
Size: 1194 4786
NspI r_CATG'y
Cuts at: 1132 1229 2738 3034 3338 4883 1132
Size: 97 1509 296 304 1545 2229
Fragments arranged by size:
2229 1545 1509 304 296 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2728 1108
Size: 1620 4360
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2345 2809 2345
Size: 464 5516
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 2604 3550 2604
Size: 946 5034
PspOMI G'GGCC_C
Cuts at: 2365 2496 2365
Size: 131 5849
PstI C_TGCA'G
Cuts at: 830 2521 3332 830
Size: 1691 811 3478
Fragments arranged by size:
3478 1691 811
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SapI GCTCTTCn'nnn_
Cuts at: 2331 2331
Size: 5980
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 2388 2517 3328 4463 5144 5335 826
Size: 219 1343 129 811 1135 681 191 1471
Fragments arranged by size:
1471 1343 1135 811 681 219 191 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3307 1872
Size: 1435 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3036 3053 4084 4985 5247 820
Size: 197 926 1093 17 1031 901 262 1553
Fragments arranged by size:
1553 1093 1031 926 901 262 197 17
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 2738 3338 1132
Size: 1606 600 3774
Fragments arranged by size:
3774 1606 600
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 2187 2259 2301 2490 2871 3419
Size: 552 939 183 72 42 189 381 548
Cuts at: 3419 513
Size: 3074
Fragments arranged by size:
3074 939 552 548 381 189 183 72
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3013 4007 4024 4177 4362 4807 325
Size: 1255 1433 994 17 153 185 445 1498
Fragments arranged by size:
1498 1433 1255 994 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2790 4226 5938
Size: 274 80 33 51 1443 813 1436 1712
Cuts at: 5938 96
Size: 138
Fragments arranged by size:
1712 1443 1436 813 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2988 3020 5940 5972 2988
Size: 32 2920 32 2996
Fragments arranged by size:
2996 2920 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3181 3181
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 3305 1870
Size: 1435 4545
XmnI GAAnn'nnTTC
Cuts at: 4109 4109
Size: 5980
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI