Restriction map for
pFN21AE3183
(Circular) (Six-base) MAPSORT of: pFN21AE3183.seq Check: 9867 from: 1 to: 5971
pFN21AE3183 5971 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 2471 2547 2676 4034 5418 5686 1632
Size: 527 312 76 129 1358 1384 268 1917
Fragments arranged by size:
1917 1384 1358 527 312 268 129 76
AfeI AGC'GCT
Cuts at: 2177 2896 2177
Size: 719 5252
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 2556 4870 5847 1203
Size: 1353 2314 977 1327
Fragments arranged by size:
2314 1353 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 3134 1166
Size: 1968 4003
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2557 3141 1184
Size: 1373 584 4014
Fragments arranged by size:
4014 1373 584
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3018 3050 3018
Size: 32 5939
AlwNI CAG_nnn'CTG
Cuts at: 2056 2196 2449 2744 3714 5286 2056
Size: 140 253 295 970 1572 2741
Fragments arranged by size:
2741 1572 970 295 253 140
ApaI G_GGCC'C
Cuts at: 2108 2121 2108
Size: 13 5958
ApaLI G'TGCA_C
Cuts at: 2542 4028 5184 2542
Size: 1486 1156 3329
Fragments arranged by size:
3329 1486 1156
ApoI r'AATT_y
Cuts at: 1493 3280 3506 3658 5767 1493
Size: 1787 226 152 2109 1697
Fragments arranged by size:
2109 1787 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 2147 3296 1870
Size: 73 164 40 1149 4545
Fragments arranged by size:
4545 1149 164 73 40
BamHI G'GATC_C
Cuts at: 2302 3301 3668 2302
Size: 999 367 4605
Fragments arranged by size:
4605 999 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 2293 3292 4747 618
Size: 325 217 627 287 219 999 1455 1842
Fragments arranged by size:
1842 1455 999 627 325 287 219 217
BanII G_rGCy'C
Cuts at: 1391 2108 2121 2784 3290 1391
Size: 717 13 663 506 4072
Fragments arranged by size:
4072 717 663 506 13
BbeI G_GCGC'C
Cuts at: 2078 2078
Size: 5971
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2553 2559 2607 3270 928
Size: 971 654 6 48 663 3629
Fragments arranged by size:
3629 971 663 654 48 6
BbvCI CC'TCA_GC
Cuts at: 2493 2640 2493
Size: 147 5824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4162 4196 1903
Size: 34 50 34 2141 34 3678
Fragments arranged by size:
3678 2141 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 3111 1548
Size: 1563 4408
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2026 2243 4582
Size: 107 122 71 631 605 354 217 2339
Cuts at: 4582 136
Size: 1525
Fragments arranged by size:
2339 1525 631 605 354 217 122 107
71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 2210 3388 2210
Size: 1178 4793
Bme1580I G_kGCm'C
Cuts at: 1790 2108 2121 2352 2546 4032 5188 1790
Size: 318 13 231 194 1486 1156 2573
Fragments arranged by size:
2573 1486 1156 318 231 194 13
BmgBI CAC'GTC
Cuts at: 1318 2557 1318
Size: 1239 4732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3702 4660 442
Size: 416 53 83 399 24 2285 958 1753
Fragments arranged by size:
2285 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2897 2997 3045 3103 4631 1239
Size: 1658 100 48 58 1528 2579
Fragments arranged by size:
2579 1658 1528 100 58 48
Bpu10I CC'TnA_GC
Cuts at: 2493 2640 2783 2493
Size: 147 143 5681
Fragments arranged by size:
5681 147 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4634 882
Size: 617 3135 2219
Fragments arranged by size:
3135 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3762 493
Size: 711 2558 2702
Fragments arranged by size:
2702 2558 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2075 3778 4160 4795
Size: 53 83 186 936 542 1703 382 635
Cuts at: 4795 275
Size: 1451
Fragments arranged by size:
1703 1451 936 635 542 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2259 2289 3018 3048 1178
Size: 30 165 30 856 30 729 30 4101
Fragments arranged by size:
4101 856 729 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2594 2864 1186
Size: 1408 270 4293
Fragments arranged by size:
4293 1408 270
BseYI C'CCAG_C
Cuts at: 2056 2231 5174 5670 2056
Size: 175 2943 496 2357
Fragments arranged by size:
2943 2357 496 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2757 2774 3176 3242 1163
Size: 573 1021 17 402 66 3892
Fragments arranged by size:
3892 1021 573 402 66 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2546 2784 3199 4032 4117 5188 2546
Size: 238 415 833 85 1071 3329
Fragments arranged by size:
3329 1071 833 415 238 85
BsmI GAATG_Cn'
Cuts at: 3482 3575 3482
Size: 93 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2121 2352 2546 2784 3199 3290
Size: 399 318 13 231 194 238 415 91
Cuts at: 3290 4032 4117 5188 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 318 238 231
194 91 85 13
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3326 844
Size: 2482 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2184 4466 4640 65
Size: 1183 936 2282 174 1396
Fragments arranged by size:
2282 1396 1183 936 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2766 4615 4802 1700
Size: 110 956 1849 187 2869
Fragments arranged by size:
2869 1849 956 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 2171 2837 3150 4031 5043 2171
Size: 666 313 881 1012 3099
Fragments arranged by size:
3099 1012 881 666 313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2319 794
Size: 1525 4446
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstXI CCAn_nnnn'nTGG
Cuts at: 3058 3058
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 2302 2916 3225 3301 3668 4053 4070 5522
Size: 330 614 309 76 367 385 17 1452
Cuts at: 5522 5608 5620 5966 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 614 385 367 346 330 309
86 76 17 12
Bsu36I CC'TnA_GG
Cuts at: 2126 2589 2126
Size: 463 5508
BtgI C'CryG_G
Cuts at: 513 1065 2164 2282 513
Size: 552 1099 118 4202
Fragments arranged by size:
4202 1099 552 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 2317 2546 2745 3094 3487 4281 4301 2317
Size: 229 199 349 393 794 20 3987
Fragments arranged by size:
3987 794 393 349 229 199 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 2968 3275 2968
Size: 307 5664
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2167 2685 3418
Size: 54 1425 50 275 18 337 518 733
Cuts at: 3418 4307 5936 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 733 518 337 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2447 2625 3901 1353
Size: 1094 178 1276 3423
Fragments arranged by size:
3423 1276 1094 178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2832 3057 4558 4932 5078 387
Size: 1064 138 1243 225 1501 374 146 1280
Fragments arranged by size:
1501 1280 1243 1064 374 225 146 138
EcoICRI GAG'CTC
Cuts at: 2782 2782
Size: 5971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2471 2547 2676 2897 2997 3045
Size: 393 527 312 76 129 221 100 48
Cuts at: 3045 3103 4034 4631 5418 5686 1239
Size: 58 931 597 787 268 1524
Fragments arranged by size:
1524 931 787 597 527 393 312 268
221 129 100 76 58 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2125 2125
Size: 5971
EcoO109I rG'GnC_Cy
Cuts at: 2104 2117 2118 2104
Size: 13 1 5957
Fragments arranged by size:
5957 13 1
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 2078 2179 2196 2898 5118 1678
Size: 400 101 17 702 2220 2531
Fragments arranged by size:
2531 2220 702 400 101 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2259 2291 2503 2535 3036
Size: 32 135 32 687 32 212 32 501
Cuts at: 3036 3068 4612 4644 4686 4718 1373
Size: 32 1544 32 42 32 2626
Fragments arranged by size:
2626 1544 687 501 212 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2884 3315 3561 669
Size: 988 281 946 431 246 3079
Fragments arranged by size:
3079 988 946 431 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KasI G'GCGC_C
Cuts at: 2074 2074
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2490 5085 5269 1305
Size: 1185 2595 184 2007
Fragments arranged by size:
2595 2007 1185 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2687 10
Size: 54 1425 343 855 3294
Fragments arranged by size:
3294 1425 855 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2502 3056 3134 3929 4288 4447 518
Size: 648 1336 554 78 795 359 159 2042
Fragments arranged by size:
2042 1336 795 648 554 359 159 78
MspA1I CmG'CkG
Cuts at: 1606 2056 2166 2639 3387 4064 5212 5457 1606
Size: 450 110 473 748 677 1148 245 2120
Fragments arranged by size:
2120 1148 748 677 473 450 245 110
NarI GG'CG_CC
Cuts at: 2075 2075
Size: 5971
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5419
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 3329 4874 1132
Size: 97 2100 1545 2229
Fragments arranged by size:
2229 2100 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2096 1348
Size: 748 5223
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3018 3050 4040 4072 5553 5585 3018
Size: 32 990 32 1481 32 3404
Fragments arranged by size:
3404 1481 990 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PspOMI G'GGCC_C
Cuts at: 2104 2117 2104
Size: 13 5958
PspXI vC'TCGA_Gb
Cuts at: 2147 2147
Size: 5971
PstI C_TGCA'G
Cuts at: 830 3323 830
Size: 2493 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2056 2639 2056
Size: 583 5388
RsrII CG'GwC_CG
Cuts at: 2267 2267
Size: 5971
SacI G_AGCT'C
Cuts at: 2784 2784
Size: 5971
SacII CC_GC'GG
Cuts at: 2167 2167
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SapI GCTCTTCn'nnn_
Cuts at: 2625 2625
Size: 5971
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 3319 4454 5135 5326 826
Size: 219 2274 1135 681 191 1471
Fragments arranged by size:
2274 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2243 2243
Size: 5971
SfoI GGC'GCC
Cuts at: 2076 2076
Size: 5971
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 2109 3298 1872
Size: 237 1189 4545
Fragments arranged by size:
4545 1189 237
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 4075 4976 5238 820
Size: 197 926 204 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 204 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5971
StyI C'CwwG_G
Cuts at: 513 1065 3216 3410 513
Size: 552 2151 194 3074
Fragments arranged by size:
3074 2151 552 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3998 4015 4168 4353 4798 325
Size: 1255 2418 17 153 185 445 1498
Fragments arranged by size:
2418 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4217 5929 96
Size: 274 80 33 51 1443 2240 1712 138
Fragments arranged by size:
2240 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5931 5963 5931
Size: 32 5939
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 2362 2197
Size: 165 5806
XhoI C'TCGA_G
Cuts at: 1943 2147 1943
Size: 204 5767
XmaI C'CCGG_G
Cuts at: 1870 2107 3296 1870
Size: 237 1189 4545
Fragments arranged by size:
4545 1189 237
XmnI GAAnn'nnTTC
Cuts at: 4100 4100
Size: 5971
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PsrI SanDI
SexAI SrfI SwaI