Restriction map for
pFN21AE3182
(Circular) (Six-base) MAPSORT of: pFN21AE3182.seq Check: 2060 from: 1 to: 5962
pFN21AE3182 5962 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2048 3305 1937
Size: 111 1257 4594
Fragments arranged by size:
4594 1257 111
Acc65I G'GTAC_C
Cuts at: 3283 3283
Size: 5962
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4025 5409 5677 1632
Size: 2393 1384 268 1917
Fragments arranged by size:
2393 1917 1384 268
AfeI AGC'GCT
Cuts at: 2372 2372
Size: 5962
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 2043 3186 4861 5838 1203
Size: 840 1143 1675 977 1327
Fragments arranged by size:
1675 1327 1143 977 840
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 2662 4691 2662
Size: 2029 3933
AleI CACnn'nnGTG
Cuts at: 1166 2612 1166
Size: 1446 4516
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2729 1184
Size: 1545 4417
AlwNI CAG_nnn'CTG
Cuts at: 2485 2749 3139 3705 5277 2485
Size: 264 390 566 1572 3170
Fragments arranged by size:
3170 1572 566 390 264
ApaLI G'TGCA_C
Cuts at: 2990 4019 5175 2990
Size: 1029 1156 3777
Fragments arranged by size:
3777 1156 1029
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 2079 2293 2438 3287 1870
Size: 73 136 214 145 849 4545
Fragments arranged by size:
4545 849 214 145 136 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2123 3283 4738 618
Size: 325 217 627 288 48 1160 1455 1842
Fragments arranged by size:
1842 1455 1160 627 325 288 217 48
BanII G_rGCy'C
Cuts at: 1391 2097 2250 2445 3281 1391
Size: 706 153 195 836 4072
Fragments arranged by size:
4072 836 706 195 153
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4991
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2081 2115 4153 4187 1903
Size: 34 144 34 2038 34 3678
Fragments arranged by size:
3678 2038 144 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3854 5064 3854
Size: 1210 4752
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2628 3003 4573
Size: 107 122 71 631 605 956 375 1570
Cuts at: 4573 136
Size: 1525
Fragments arranged by size:
1570 1525 956 631 605 375 122 107
71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 3164 3379 3164
Size: 215 5747
Bme1580I G_kGCm'C
Cuts at: 1790 2078 2994 4023 5179 1790
Size: 288 916 1029 1156 2573
Fragments arranged by size:
2573 1156 1029 916 288
BmgBI CAC'GTC
Cuts at: 1318 2797 1318
Size: 1479 4483
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2920 3693 4651
Size: 416 53 83 399 24 1503 773 958
Cuts at: 4651 442
Size: 1753
Fragments arranged by size:
1753 1503 958 773 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2929 3272 4622 1239
Size: 977 713 343 1350 2579
Fragments arranged by size:
2579 1350 977 713 343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4087 4952 5250 4087
Size: 865 298 4799
Fragments arranged by size:
4799 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2558 4625 882
Size: 617 1059 2067 2219
Fragments arranged by size:
2219 2067 1059 617
BsaAI yAC'GTr
Cuts at: 493 1204 3753 493
Size: 711 2549 2702
Fragments arranged by size:
2702 2549 711
BsaBI GATnn'nnATC
Cuts at: 2894 5956 2894
Size: 3062 2900
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3072 3769 4151 4786
Size: 53 83 186 936 1539 697 382 635
Cuts at: 4786 275
Size: 1451
Fragments arranged by size:
1539 1451 936 697 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5737
Fragments arranged by size:
5737 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 1186
Size: 1018 4944
BseYI C'CCAG_C
Cuts at: 2029 2069 2617 2681 3157 5165 5661 2029
Size: 40 548 64 476 2008 496 2330
Fragments arranged by size:
2330 2008 548 496 476 64 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5389
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2240 2427 3412 4173 5201 1540
Size: 275 186 239 187 985 761 1028 2301
Fragments arranged by size:
2301 1028 985 761 275 239 187 186
BsiHKAI G_wGCw'C
Cuts at: 2250 2466 2559 2802 2865 2994 4023 4108 5179
Size: 216 93 243 63 129 1029 85 1071
Cuts at: 5179 2250
Size: 3033
Fragments arranged by size:
3033 1071 1029 243 216 129 93 85
63
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
BsmBI CGTCTCn'nnnn_
Cuts at: 2264 2264
Size: 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2097 2250 2445 2466 2559 2802
Size: 399 288 19 153 195 21 93 243
Cuts at: 2802 2865 2994 3281 4023 4108 5179 1391
Size: 63 129 287 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 288 287 243 195
153 129 93 85 63 21 19
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 3851 5581 3851
Size: 1730 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2337 3317 844
Size: 1493 980 3489
Fragments arranged by size:
3489 1493 980
BsrBI CCG'CTC
Cuts at: 1394 1823 2340 2967 3015 3151 3849 1394
Size: 429 517 627 48 136 698 3507
Fragments arranged by size:
3507 698 627 517 429 136 48
BsrDI GCAATG_nn'
Cuts at: 65 1248 4457 4631 65
Size: 1183 3209 174 1396
Fragments arranged by size:
3209 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 4606 4793 1700
Size: 110 425 2371 187 2869
Fragments arranged by size:
2869 2371 425 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5962
BssHII G'CGCG_C
Cuts at: 1925 2201 1925
Size: 276 5686
BssSI C'ACGA_G
Cuts at: 2243 4022 5034 2243
Size: 1779 1012 3171
Fragments arranged by size:
3171 1779 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3174 794
Size: 2380 3582
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstXI CCAn_nnnn'nTGG
Cuts at: 2284 2791 2284
Size: 507 5455
BstYI r'GATC_y
Cuts at: 1972 2745 2889 3292 3659 4044 4061 5513 5599
Size: 773 144 403 367 385 17 1452 86
Cuts at: 5599 5611 5957 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 773 403 385 367 346 144
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2937 2937
Size: 5962
BtgI C'CryG_G
Cuts at: 513 1065 2020 2421 3258 513
Size: 552 955 401 837 3217
Fragments arranged by size:
3217 955 837 552 401
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 2557 2692 2992 3478 4272 4292 2557
Size: 135 300 486 794 20 4227
Fragments arranged by size:
4227 794 486 300 135 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2959 2994 529
Size: 35 2395 35 3497
Fragments arranged by size:
3497 2395 35 35
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2237 2274 2286
Size: 54 1425 50 275 18 407 37 12
Cuts at: 2286 2394 2424 3409 4298 5927 8
Size: 108 30 985 889 1629 43
Fragments arranged by size:
1629 1425 985 889 407 275 108 54
50 43 37 30 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2237 2424 3409 1537
Size: 275 425 187 985 4090
Fragments arranged by size:
4090 985 425 275 187
EarI CTCTTCn'nnn_
Cuts at: 1353 2255 2587 3892 1353
Size: 902 332 1305 3423
Fragments arranged by size:
3423 1305 902 332
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2248 2248
Size: 5962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2929 3272 4025 4622 5409 5677
Size: 393 584 713 343 753 597 787 268
Cuts at: 5677 1239
Size: 1524
Fragments arranged by size:
1524 787 753 713 597 584 393 343
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2318 2318
Size: 5962
EcoO109I rG'GnC_Cy
Cuts at: 2629 2629
Size: 5962
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 5962
FspI TGC'GCA
Cuts at: 2318 2636 4468 2318
Size: 318 1832 3812
Fragments arranged by size:
3812 1832 318
HaeII r_GCGC'y
Cuts at: 1678 2175 2374 2451 5109 1678
Size: 497 199 77 2658 2531
Fragments arranged by size:
2658 2531 497 199 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2648 2680 4603 4635 4677
Size: 32 135 32 1076 32 1923 32 42
Cuts at: 4677 4709 1373
Size: 32 2626
Fragments arranged by size:
2626 1923 1076 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2049 3306 3552 669
Size: 988 281 111 1257 246 3079
Fragments arranged by size:
3079 1257 988 281 246 111
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 3287 3287
Size: 5962
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 3186 5838 3186
Size: 2652 3310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2646 2679 3126 5076 5260 1305
Size: 1341 33 447 1950 184 2007
Fragments arranged by size:
2007 1950 1341 447 184 33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2288 10
Size: 54 1425 343 456 3684
Fragments arranged by size:
3684 1425 456 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2612 3920 4279 4438 518
Size: 648 1446 1308 359 159 2042
Fragments arranged by size:
2042 1446 1308 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 3136 3378 4055 5203 5448 1606
Size: 416 1114 242 677 1148 245 2120
Fragments arranged by size:
2120 1148 1114 677 416 245 242
NaeI GCC'GGC
Cuts at: 2237 2237
Size: 5962
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5410
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NgoMIV G'CCGG_C
Cuts at: 2235 2235
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5962
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2047 3320 4865 1132
Size: 97 818 1273 1545 2229
Fragments arranged by size:
2229 1545 1273 818 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5962
PciI A'CATG_T
Cuts at: 2043 4861 2043
Size: 2818 3144
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 5544 5576 4031
Size: 32 1481 32 4417
Fragments arranged by size:
4417 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2187 3314 830
Size: 1357 1127 3478
Fragments arranged by size:
3478 1357 1127
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 3136 3136
Size: 5962
RsrII CG'GwC_CG
Cuts at: 2668 2668
Size: 5962
SacI G_AGCT'C
Cuts at: 2250 2250
Size: 5962
SacII CC_GC'GG
Cuts at: 2023 2023
Size: 5962
SalI G'TCGA_C
Cuts at: 1936 2047 3304 1936
Size: 111 1257 4594
Fragments arranged by size:
4594 1257 111
SapI GCTCTTCn'nnn_
Cuts at: 2255 2587 2255
Size: 332 5630
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 2549 4210 5922 2549
Size: 1661 1712 2589
Fragments arranged by size:
2589 1712 1661
SfcI C'TryA_G
Cuts at: 826 1045 2183 2477 3310 4445 5126 5317 826
Size: 219 1138 294 833 1135 681 191 1471
Fragments arranged by size:
1471 1138 1135 833 681 294 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2628 2628
Size: 5962
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 2081 2295 3289 1872
Size: 209 214 994 4545
Fragments arranged by size:
4545 994 214 209
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4066 4967 5229 820
Size: 197 926 2123 901 262 1553
Fragments arranged by size:
2123 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 2507 1517
Size: 990 4972
StyI C'CwwG_G
Cuts at: 513 1065 2518 2775 3401 513
Size: 552 1453 257 626 3074
Fragments arranged by size:
3074 1453 626 552 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2620 2685 2854 3989 4006 4159 4344
Size: 1255 1040 65 169 1135 17 153 185
Cuts at: 4344 4789 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1135 1040 445 185 169 153
65 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 4208 5920
Size: 274 80 33 51 1443 570 1661 1712
Cuts at: 5920 96
Size: 138
Fragments arranged by size:
1712 1661 1443 570 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 3083 1316
Size: 1767 4195
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2614 2614
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 2079 2293 3287 1870
Size: 209 214 994 4545
Fragments arranged by size:
4545 994 214 209
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI SanDI SexAI SfoI SrfI
SwaI