Restriction map for
pFN21AE3178
(Circular) (Six-base) MAPSORT of: pFN21AE3178.seq Check: 2598 from: 1 to: 5950
pFN21AE3178 5950 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3293 1937
Size: 1356 4594
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2214 3177 4013 5397 5665 1632
Size: 416 166 963 836 1384 268 1917
Fragments arranged by size:
1917 1384 963 836 416 268 166
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 2913 4849 5826 1203
Size: 1710 1936 977 1327
Fragments arranged by size:
1936 1710 1327 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AlwNI CAG_nnn'CTG
Cuts at: 3693 5265 3693
Size: 1572 4378
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 2669 3259 3485 3637 5746 1493
Size: 1176 590 226 152 2109 1697
Fragments arranged by size:
2109 1697 1176 590 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2499 4504 160
Size: 872 1467 2005 1606
Fragments arranged by size:
2005 1606 1467 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 3275 1870
Size: 73 1332 4545
Fragments arranged by size:
4545 1332 73
AvrII C'CTAG_G
Cuts at: 2866 2866
Size: 5950
BamHI G'GATC_C
Cuts at: 2305 3280 3647 2305
Size: 975 367 4608
Fragments arranged by size:
4608 975 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 3135 3271 4726 618
Size: 325 217 627 251 1097 136 1455 1842
Fragments arranged by size:
1842 1455 1097 627 325 251 217 136
BanII G_rGCy'C
Cuts at: 1391 2300 3269 1391
Size: 909 969 4072
Fragments arranged by size:
4072 969 909
BbeI G_GCGC'C
Cuts at: 3139 3139
Size: 5950
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2793 2847 928
Size: 971 894 54 4031
Fragments arranged by size:
4031 971 894 54
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4141 4175 1903
Size: 34 50 34 2120 34 3678
Fragments arranged by size:
3678 2120 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BfrBI ATG'CAT
Cuts at: 2123 2603 2123
Size: 480 5470
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2966 4561 136
Size: 107 122 71 631 605 1294 1595 1525
Fragments arranged by size:
1595 1525 1294 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3025 5945 3025
Size: 2920 3030
BlpI GC'TnA_GC
Cuts at: 3367 3367
Size: 5950
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2718 4011 5167 1790
Size: 253 675 1293 1156 2573
Fragments arranged by size:
2573 1293 1156 675 253
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5950
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 4639 442
Size: 416 53 83 399 24 2264 958 1753
Fragments arranged by size:
2264 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2634 2963 4610 1239
Size: 1395 329 1647 2579
Fragments arranged by size:
2579 1647 1395 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4613 882
Size: 617 3114 2219
Fragments arranged by size:
3114 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3741 493
Size: 711 2537 2702
Fragments arranged by size:
2702 2537 711
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3136 3757 4139 4774
Size: 53 83 186 936 1603 621 382 635
Cuts at: 4774 275
Size: 1451
Fragments arranged by size:
1603 1451 936 635 621 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2016 4383 4781 5055 5202 5613 2016
Size: 2367 398 274 147 411 2353
Fragments arranged by size:
2367 2353 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5725
Fragments arranged by size:
5725 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5950
BseYI C'CCAG_C
Cuts at: 2936 3179 5153 5649 2936
Size: 243 1974 496 3237
Fragments arranged by size:
3237 1974 496 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5377
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4011 4096 5167 4011
Size: 85 1071 4794
Fragments arranged by size:
4794 1071 85
BsmI GAATG_Cn'
Cuts at: 3461 3554 3461
Size: 93 5857
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2300 2718 3269 4011 4096 5167
Size: 399 253 257 418 551 742 85 1071
Cuts at: 5167 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 551 418 399 257 253
85
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3305 844
Size: 2461 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2083 3837 1394
Size: 429 260 1754 3507
Fragments arranged by size:
3507 1754 429 260
BsrDI GCAATG_nn'
Cuts at: 65 1248 4445 4619 65
Size: 1183 3197 174 1396
Fragments arranged by size:
3197 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 4594 4781 1700
Size: 110 225 2559 187 2869
Fragments arranged by size:
2869 2559 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2038 794
Size: 1244 4706
BstBI TT'CG_AA
Cuts at: 2887 4754 2887
Size: 1867 4083
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstYI r'GATC_y
Cuts at: 1972 2305 3025 3280 3647 4032 4049 5501 5587
Size: 333 720 255 367 385 17 1452 86
Cuts at: 5587 5599 5945 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 720 385 367 346 333 255
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2301 2301
Size: 5950
BtgI C'CryG_G
Cuts at: 513 1065 3042 513
Size: 552 1977 3421
Fragments arranged by size:
3421 1977 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 3190 3466 4260 4280 3190
Size: 276 794 20 4860
Fragments arranged by size:
4860 794 276 20
ClaI AT'CG_AT
Cuts at: 1554 2565 1554
Size: 1011 4939
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2298 2333 529
Size: 35 1734 35 4146
Fragments arranged by size:
4146 1734 35 35
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3397 4286 5915
Size: 54 1425 50 275 18 1567 889 1629
Cuts at: 5915 8
Size: 43
Fragments arranged by size:
1629 1567 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3880 1353
Size: 2527 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2600 4537 4911 5057 387
Size: 1064 138 1011 1937 374 146 1280
Fragments arranged by size:
1937 1280 1064 1011 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2214 2634 2963 3177 4013 4610
Size: 393 416 166 420 329 214 836 597
Cuts at: 4610 5397 5665 1239
Size: 787 268 1524
Fragments arranged by size:
1524 836 787 597 420 416 393 329
268 214 166
EcoRI G'AATT_C
Cuts at: 2669 3259 3637 2669
Size: 590 378 4982
Fragments arranged by size:
4982 590 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2216 2216
Size: 5950
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 2687 3139 5097 1678
Size: 1009 452 1958 2531
Fragments arranged by size:
2531 1958 1009 452
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4591 4623 4665 4697 1373
Size: 32 135 32 3019 32 42 32 2626
Fragments arranged by size:
3019 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 3540 669
Size: 988 281 1356 246 3079
Fragments arranged by size:
3079 1356 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KasI G'GCGC_C
Cuts at: 3135 3135
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5064 5248 1305
Size: 3759 184 2007
Fragments arranged by size:
3759 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4128
Fragments arranged by size:
4128 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2364 3908 4267 4426 518
Size: 648 1198 1544 359 159 2042
Fragments arranged by size:
2042 1544 1198 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3366 4043 5191 5436 1606
Size: 1760 677 1148 245 2120
Fragments arranged by size:
2120 1760 1148 677 245
NarI GG'CG_CC
Cuts at: 3136 3136
Size: 5950
NcoI C'CATG_G
Cuts at: 513 1065 3042 513
Size: 552 1977 3421
Fragments arranged by size:
3421 1977 552
NdeI CA'TA_TG
Cuts at: 387 2605 2733 387
Size: 2218 128 3604
Fragments arranged by size:
3604 2218 128
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NsiI A_TGCA'T
Cuts at: 2125 2605 2125
Size: 480 5470
NspI r_CATG'y
Cuts at: 1132 1229 2127 2494 2917 3308 4853 1132
Size: 97 898 367 423 391 1545 2229
Fragments arranged by size:
2229 1545 898 423 391 367 97
PasI CC'CwG_GG
Cuts at: 1348 3192 1348
Size: 1844 4106
PciI A'CATG_T
Cuts at: 2913 4849 2913
Size: 1936 4014
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5950
PfoI T'CCnGG_A
Cuts at: 2100 2100
Size: 5950
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PstI C_TGCA'G
Cuts at: 830 3302 830
Size: 2472 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SexAI A'CCwGG_T
Cuts at: 2640 2640
Size: 5950
SfcI C'TryA_G
Cuts at: 826 1045 3298 4433 5114 5305 826
Size: 219 2253 1135 681 191 1471
Fragments arranged by size:
2253 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3137 3137
Size: 5950
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 2494 3308 1132
Size: 1362 814 3774
Fragments arranged by size:
3774 1362 814
SspI AAT'ATT
Cuts at: 5 52 2450 3874 5
Size: 47 2398 1424 2081
Fragments arranged by size:
2398 2081 1424 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5950
StyI C'CwwG_G
Cuts at: 513 1065 2866 3042 3389 513
Size: 552 1801 176 347 3074
Fragments arranged by size:
3074 1801 552 347 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3977 3994 4147 4332 4777 325
Size: 1255 2397 17 153 185 445 1498
Fragments arranged by size:
2397 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4196 5908 96
Size: 274 80 33 51 1443 2219 1712 138
Fragments arranged by size:
2219 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5910 5942 5910
Size: 32 5918
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2578 3049 2578
Size: 471 5479
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 4079 4079
Size: 5950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SrfI SwaI