Restriction map for
pFN21AE3171
(Circular) (Six-base) MAPSORT of: pFN21AE3171.seq Check: 904 from: 1 to: 5914
pFN21AE3171 5914 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2892 3724 278
Size: 53 83 186 936 1356 832 2468
Fragments arranged by size:
2468 1356 936 832 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2049 3257 1937
Size: 112 1208 4594
Fragments arranged by size:
4594 1208 112
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2646 3977 5361 5629 1632
Size: 1014 1331 1384 268 1917
Fragments arranged by size:
1917 1384 1331 1014 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 4813 5790 1203
Size: 3610 977 1327
Fragments arranged by size:
3610 1327 977
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 2776 4643 2776
Size: 1867 4047
AleI CACnn'nnGTG
Cuts at: 1166 2066 1166
Size: 900 5014
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2198 1184
Size: 1014 4900
AlwNI CAG_nnn'CTG
Cuts at: 3657 5229 3657
Size: 1572 4342
ApaLI G'TGCA_C
Cuts at: 3971 5127 3971
Size: 1156 4758
ApoI r'AATT_y
Cuts at: 1493 2596 3223 3449 3601 5710 1493
Size: 1103 627 226 152 2109 1697
Fragments arranged by size:
2109 1697 1103 627 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 2821 3239 1870
Size: 73 878 418 4545
Fragments arranged by size:
4545 878 418 73
BamHI G'GATC_C
Cuts at: 2587 3244 3611 2587
Size: 657 367 4890
Fragments arranged by size:
4890 657 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3235 4690 618
Size: 325 217 627 1448 1455 1842
Fragments arranged by size:
1842 1455 1448 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3233 1391
Size: 1842 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2909 928
Size: 971 1010 3933
Fragments arranged by size:
3933 1010 971
BbvCI CC'TCA_GC
Cuts at: 2910 2910
Size: 5914
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4105 4139 1903
Size: 34 2168 34 3678
Fragments arranged by size:
3678 2168 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5914
BfrBI ATG'CAT
Cuts at: 3035 3035
Size: 5914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4525 136
Size: 107 122 71 631 605 2853 1525
Fragments arranged by size:
2853 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5909 5909
Size: 5914
BlpI GC'TnA_GC
Cuts at: 3331 3331
Size: 5914
Bme1580I G_kGCm'C
Cuts at: 1790 3975 5131 1790
Size: 2185 1156 2573
Fragments arranged by size:
2573 2185 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5914
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3123 3645 4603
Size: 416 53 83 399 24 1706 522 958
Cuts at: 4603 442
Size: 1753
Fragments arranged by size:
1753 1706 958 522 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2778 4574 1239
Size: 1022 517 1796 2579
Fragments arranged by size:
2579 1796 1022 517
Bpu10I CC'TnA_GC
Cuts at: 2910 2910
Size: 5914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 2429 4039 4904 5202 2300
Size: 129 1610 865 298 3012
Fragments arranged by size:
3012 1610 865 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4577 882
Size: 617 3078 2219
Fragments arranged by size:
3078 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3705 493
Size: 711 2501 2702
Fragments arranged by size:
2702 2501 711
BsaBI GATnn'nnATC
Cuts at: 3156 5908 3156
Size: 2752 3162
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2889 3721 4103 4738
Size: 53 83 186 936 1356 832 382 635
Cuts at: 4738 275
Size: 1451
Fragments arranged by size:
1451 1356 936 832 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4347 4745 5019 5166 5577 4347
Size: 398 274 147 411 4684
Fragments arranged by size:
4684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2152 2182 2751 2781 1178
Size: 30 165 30 749 30 569 30 4311
Fragments arranged by size:
4311 749 569 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2032 2288 2867 1186
Size: 846 256 579 4233
Fragments arranged by size:
4233 846 579 256
BseYI C'CCAG_C
Cuts at: 5117 5613 5117
Size: 496 5418
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3364 4125 5153 1540
Size: 275 186 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2063 2106 2877 3975 4060 5131 2063
Size: 43 771 1098 85 1071 2846
Fragments arranged by size:
2846 1098 1071 771 85 43
BsmI GAATG_Cn'
Cuts at: 3425 3518 3425
Size: 93 5821
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2106 2877 3233 3975 4060 5131
Size: 399 273 43 771 356 742 85 1071
Cuts at: 5131 1391
Size: 2174
Fragments arranged by size:
2174 1071 771 742 399 356 273 85
43
BspEI T'CCGG_A
Cuts at: 5577 5577
Size: 5914
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3269 844
Size: 2425 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3801 1394
Size: 429 1978 3507
Fragments arranged by size:
3507 1978 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2248 4409 4583 65
Size: 1183 1000 2161 174 1396
Fragments arranged by size:
2161 1396 1183 1000 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 2231 4558 4745 1700
Size: 110 201 220 2327 187 2869
Fragments arranged by size:
2869 2327 220 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5914
BssSI C'ACGA_G
Cuts at: 3974 4986 3974
Size: 1012 4902
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5914
BstBI TT'CG_AA
Cuts at: 4718 4718
Size: 5914
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5914
BstXI CCAn_nnnn'nTGG
Cuts at: 2441 2441
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 2208 2587 3244 3611 3996 4013 5465 5551
Size: 236 379 657 367 385 17 1452 86
Cuts at: 5551 5563 5909 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 657 385 379 367 346 236
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2499 2499
Size: 5914
BtgI C'CryG_G
Cuts at: 513 1065 2004 2035 2054 513
Size: 552 939 31 19 4373
Fragments arranged by size:
4373 939 552 31 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2933 487
Size: 683 56 1707 3468
Fragments arranged by size:
3468 1707 683 56
BtsI GCAGTG_nn'
Cuts at: 3430 4224 4244 3430
Size: 794 20 5100
Fragments arranged by size:
5100 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 3218 3218
Size: 5914
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4921 809
Size: 507 3605 1802
Fragments arranged by size:
3605 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2233 2359 2937
Size: 54 1425 50 275 18 403 126 578
Cuts at: 2937 3361 4250 5879 8
Size: 424 889 1629 43
Fragments arranged by size:
1629 1425 889 578 424 403 275 126
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3361 1537
Size: 275 1549 4090
Fragments arranged by size:
4090 1549 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2343 3844 1353
Size: 990 1501 3423
Fragments arranged by size:
3423 1501 990
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2099 2240 4501 4875 5021 387
Size: 1064 138 510 141 2261 374 146 1280
Fragments arranged by size:
2261 1280 1064 510 374 146 141 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2646 2778 3977 4574 5361 5629
Size: 393 629 385 132 1199 597 787 268
Cuts at: 5629 1239
Size: 1524
Fragments arranged by size:
1524 1199 787 629 597 393 385 268
132
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2225 2882 2084
Size: 141 657 5116
Fragments arranged by size:
5116 657 141
EcoRI G'AATT_C
Cuts at: 3223 3601 3223
Size: 378 5536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2651 2651
Size: 5914
FseI GG_CCGG'CC
Cuts at: 2235 2235
Size: 5914
FspI TGC'GCA
Cuts at: 2174 4420 2174
Size: 2246 3668
HaeII r_GCGC'y
Cuts at: 1678 2929 5061 1678
Size: 1251 2132 2531
Fragments arranged by size:
2531 2132 1251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2604 2636 2762 2794 2841
Size: 32 135 32 1032 32 126 32 47
Cuts at: 2841 2873 4555 4587 4629 4661 1373
Size: 32 1682 32 42 32 2626
Fragments arranged by size:
2626 1682 1032 135 126 47 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3504 669
Size: 988 281 1320 246 3079
Fragments arranged by size:
3079 1320 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5914
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 3513 3513
Size: 5914
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2812 5028 5212 1305
Size: 1507 2216 184 2007
Fragments arranged by size:
2216 2007 1507 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2361 10
Size: 54 1425 343 529 3563
Fragments arranged by size:
3563 1425 529 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 3872 4231 4390 518
Size: 648 900 1806 359 159 2042
Fragments arranged by size:
2042 1806 900 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2056 3330 4007 5155 5400 1606
Size: 431 19 1274 677 1148 245 2120
Fragments arranged by size:
2120 1274 1148 677 431 245 19
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 5914
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4423
Fragments arranged by size:
4423 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5914
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 5914
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5914
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NsiI A_TGCA'T
Cuts at: 3037 3037
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 2982 3272 4817 1132
Size: 97 1753 290 1545 2229
Fragments arranged by size:
2229 1753 1545 290 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5914
PciI A'CATG_T
Cuts at: 4813 4813
Size: 5914
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5914
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 2679 3484 2679
Size: 805 5109
PstI C_TGCA'G
Cuts at: 830 2715 3266 830
Size: 1885 551 3478
Fragments arranged by size:
3478 1885 551
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
SacII CC_GC'GG
Cuts at: 2038 2057 2038
Size: 19 5895
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SbfI CC_TGCA'GG
Cuts at: 3266 3266
Size: 5914
ScaI AGT'ACT
Cuts at: 4162 5874 4162
Size: 1712 4202
SfcI C'TryA_G
Cuts at: 826 1045 2711 3262 4397 5078 5269 826
Size: 219 1666 551 1135 681 191 1471
Fragments arranged by size:
1666 1471 1135 681 551 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 3241 1872
Size: 1369 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 2408 4018 4919 5181 820
Size: 197 926 336 129 1610 901 262 1553
Fragments arranged by size:
1610 1553 926 901 336 262 197 129
SnaBI TAC'GTA
Cuts at: 493 3705 493
Size: 3212 2702
SpeI A'CTAG_T
Cuts at: 152 2384 152
Size: 2232 3682
SphI G_CATG'C
Cuts at: 1132 2982 3272 1132
Size: 1850 290 3774
Fragments arranged by size:
3774 1850 290
SspI AAT'ATT
Cuts at: 5 52 3838 5
Size: 47 3786 2081
Fragments arranged by size:
3786 2081 47
StuI AGG'CCT
Cuts at: 1517 2220 1517
Size: 703 5211
StyI C'CwwG_G
Cuts at: 513 1065 2004 2121 2440 3012 3353 513
Size: 552 939 117 319 572 341 3074
Fragments arranged by size:
3074 939 572 552 341 319 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3941 3958 4111 4296 4741 325
Size: 1255 2361 17 153 185 445 1498
Fragments arranged by size:
2361 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4160 5872 96
Size: 274 80 33 51 1443 2183 1712 138
Fragments arranged by size:
2183 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2152 2184 2451 2483 2751 2783 5874 5906 2152
Size: 32 267 32 268 32 3091 32 2160
Fragments arranged by size:
3091 2160 268 267 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5914
XbaI T'CTAG_A
Cuts at: 3250 3250
Size: 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5914
XmaI C'CCGG_G
Cuts at: 1870 3239 1870
Size: 1369 4545
XmnI GAAnn'nnTTC
Cuts at: 2417 4043 2417
Size: 1626 4288
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2890 3722 276
Size: 53 83 186 936 1356 832 2468
Fragments arranged by size:
2468 1356 936 832 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV
FspAI KasI NarI NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI