Restriction map for
pFN21AE3167
(Circular) (Six-base) MAPSORT of: pFN21AE3167.seq Check: 5465 from: 1 to: 5881
pFN21AE3167 5881 bp
With 182 enzymes: SgfI *
March 28, 2012 13:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3224 1937
Size: 1287 4594
Acc65I G'GTAC_C
Cuts at: 2333 3202 2333
Size: 869 5012
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3944 5328 5596 1632
Size: 2312 1384 268 1917
Fragments arranged by size:
2312 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5684
AflIII A'CryG_T
Cuts at: 1203 4780 5757 1203
Size: 3577 977 1327
Fragments arranged by size:
3577 1327 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 2285 1166
Size: 1119 4762
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 2353 3624 5196 2353
Size: 1271 1572 3038
Fragments arranged by size:
3038 1572 1271
ApaI G_GGCC'C
Cuts at: 2074 2074
Size: 5881
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 2028 2744 3190 3416 3568 5677 1493
Size: 535 716 446 226 152 2109 1697
Fragments arranged by size:
2109 1697 716 535 446 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 3206 1870
Size: 73 1263 4545
Fragments arranged by size:
4545 1263 73
AvrII C'CTAG_G
Cuts at: 2191 2191
Size: 5881
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2317 2350 2317
Size: 33 5848
BamHI G'GATC_C
Cuts at: 2607 3211 3578 2607
Size: 604 367 4910
Fragments arranged by size:
4910 604 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2333 3202 4657 618
Size: 325 217 627 463 83 869 1455 1842
Fragments arranged by size:
1842 1455 869 627 463 325 217 83
BanII G_rGCy'C
Cuts at: 1391 2074 2466 2664 3200 1391
Size: 683 392 198 536 4072
Fragments arranged by size:
4072 683 536 392 198
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4910
BbvCI CC'TCA_GC
Cuts at: 2115 2826 2115
Size: 711 5170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4072 4106 1903
Size: 34 2135 34 3678
Fragments arranged by size:
3678 2135 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 2111 4492
Size: 107 122 71 631 605 406 33 2381
Cuts at: 4492 136
Size: 1525
Fragments arranged by size:
2381 1525 631 605 406 122 107 71
33
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 3298 3298
Size: 5881
Bme1580I G_kGCm'C
Cuts at: 1790 2074 2253 3942 5098 1790
Size: 284 179 1689 1156 2573
Fragments arranged by size:
2573 1689 1156 284 179
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3612 4570 442
Size: 416 53 83 399 24 2195 958 1753
Fragments arranged by size:
2195 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 4541 1239
Size: 998 2304 2579
Fragments arranged by size:
2579 2304 998
Bpu10I CC'TnA_GC
Cuts at: 2115 2826 2115
Size: 711 5170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2678 4006 4871 5169 2678
Size: 1328 865 298 3390
Fragments arranged by size:
3390 1328 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3002 4544 882
Size: 617 1503 1542 2219
Fragments arranged by size:
2219 1542 1503 617
BsaAI yAC'GTr
Cuts at: 493 1204 3672 493
Size: 711 2468 2702
Fragments arranged by size:
2702 2468 711
BsaBI GATnn'nnATC
Cuts at: 2558 5875 2558
Size: 3317 2564
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3688 4070 4705 275
Size: 53 83 186 936 2155 382 635 1451
Fragments arranged by size:
2155 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2793 4314 4712 4986 5133 5544 2793
Size: 1521 398 274 147 411 3130
Fragments arranged by size:
3130 1521 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5656
Fragments arranged by size:
5656 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2003 2156 2780 1186
Size: 817 153 624 4287
Fragments arranged by size:
4287 817 624 153
BseYI C'CCAG_C
Cuts at: 2911 5084 5580 2911
Size: 2173 496 3212
Fragments arranged by size:
3212 2173 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2492 1163
Size: 573 756 4552
Fragments arranged by size:
4552 756 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3331 4092 5120 1540
Size: 275 186 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2664 3942 4027 5098 2664
Size: 1278 85 1071 3447
Fragments arranged by size:
3447 1278 1071 85
BsmI GAATG_Cn'
Cuts at: 2197 2730 3392 3485 2197
Size: 533 662 93 4593
Fragments arranged by size:
4593 662 533 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2253 2466 2664 3200 3942 4027
Size: 399 284 179 213 198 536 742 85
Cuts at: 4027 5098 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 536 399 284 213 198
179 85
BspEI T'CCGG_A
Cuts at: 2793 5544 2793
Size: 2751 3130
BspHI T'CATG_A
Cuts at: 3770 5500 3770
Size: 1730 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3236 844
Size: 2392 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3768 1394
Size: 429 1945 3507
Fragments arranged by size:
3507 1945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2688 2931 4376 4550 65
Size: 1183 1440 243 1445 174 1396
Fragments arranged by size:
1445 1440 1396 1183 243 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2226 4525 4712 1700
Size: 110 416 2299 187 2869
Fragments arranged by size:
2869 2299 416 187 110
BsrGI T'GTAC_A
Cuts at: 96 3135 3170 96
Size: 3039 35 2807
Fragments arranged by size:
3039 2807 35
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 2048 3941 4953 2048
Size: 1893 1012 2976
Fragments arranged by size:
2976 1893 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2203 794
Size: 1409 4472
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstXI CCAn_nnnn'nTGG
Cuts at: 2761 2761
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 2349 2607 3211 3578 3963 3980 5432 5518
Size: 377 258 604 367 385 17 1452 86
Cuts at: 5518 5530 5876 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 604 385 377 367 346 258
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2283 513
Size: 552 939 279 4111
Fragments arranged by size:
4111 939 552 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3006 487
Size: 683 56 1780 3362
Fragments arranged by size:
3362 1780 683 56
BtsI GCAGTG_nn'
Cuts at: 2201 3397 4191 4211 2201
Size: 1196 794 20 3871
Fragments arranged by size:
3871 1196 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 3185 3185
Size: 5881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3056 4888 809
Size: 507 1740 1832 1802
Fragments arranged by size:
1832 1802 1740 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846
Size: 54 1425 50 275 18 1498 889 1629
Cuts at: 5846 8
Size: 43
Fragments arranged by size:
1629 1498 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3328 1537
Size: 275 1516 4090
Fragments arranged by size:
4090 1516 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2793 2845 3811 1353
Size: 1440 52 966 3423
Fragments arranged by size:
3423 1440 966 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2894 4468 4842 4988 387
Size: 1064 138 1305 1574 374 146 1280
Fragments arranged by size:
1574 1305 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2662 2662
Size: 5881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 3944 4541 5328 5596 1239
Size: 393 605 1707 597 787 268 1524
Fragments arranged by size:
1707 1524 787 605 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2138 2328 2138
Size: 190 5691
EcoO109I rG'GnC_Cy
Cuts at: 2070 2119 2070
Size: 49 5832
EcoRI G'AATT_C
Cuts at: 2028 3190 3568 2028
Size: 1162 378 4341
Fragments arranged by size:
4341 1162 378
EcoRV GAT'ATC
Cuts at: 2560 2560
Size: 5881
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 5028 1678
Size: 3350 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2592 2624 2804 2836 2920
Size: 32 135 32 1020 32 180 32 84
Cuts at: 2920 2952 4522 4554 4596 4628 1373
Size: 32 1570 32 42 32 2626
Fragments arranged by size:
2626 1570 1020 180 135 84 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5881
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 2337 3206 2337
Size: 869 5012
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2452 4995 5179 1305
Size: 1147 2543 184 2007
Fragments arranged by size:
2543 2007 1147 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4059
Fragments arranged by size:
4059 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 3839 4198 4357 518
Size: 648 1119 1554 359 159 2042
Fragments arranged by size:
2042 1554 1119 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2237 2825 2911 3297 3974 5122 5367 1606
Size: 631 588 86 386 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 631 588 386 245 86
NcoI C'CATG_G
Cuts at: 513 1065 2004 2283 513
Size: 552 939 279 4111
Fragments arranged by size:
4111 939 552 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 2215 3177 3239 4784 1132
Size: 97 986 962 62 1545 2229
Fragments arranged by size:
2229 1545 986 962 97 62
PasI CC'CwG_GG
Cuts at: 1348 2466 2754 1348
Size: 1118 288 4475
Fragments arranged by size:
4475 1118 288
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5881
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5881
PsiI TTA'TAA
Cuts at: 3451 3451
Size: 5881
PspOMI G'GGCC_C
Cuts at: 2070 2070
Size: 5881
PstI C_TGCA'G
Cuts at: 830 3233 830
Size: 2403 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 2237 2825 2911 2237
Size: 588 86 5207
Fragments arranged by size:
5207 588 86
SacI G_AGCT'C
Cuts at: 2664 2664
Size: 5881
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 2985 4129 5841 2985
Size: 1144 1712 3025
Fragments arranged by size:
3025 1712 1144
SfcI C'TryA_G
Cuts at: 826 1045 3229 4364 5045 5236 826
Size: 219 2184 1135 681 191 1471
Fragments arranged by size:
2184 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 3208 1872
Size: 1336 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2657 3985 4886 5148 820
Size: 197 926 714 1328 901 262 1553
Fragments arranged by size:
1553 1328 926 901 714 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 2392 152
Size: 2240 3641
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 2323 1517
Size: 806 5075
StyI C'CwwG_G
Cuts at: 513 1065 2004 2174 2191 2283 2682 3320 513
Size: 552 939 170 17 92 399 638 3074
Fragments arranged by size:
3074 939 638 552 399 170 92 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2458 3908 3925 4078 4263 4708 325
Size: 1255 878 1450 17 153 185 445 1498
Fragments arranged by size:
1498 1450 1255 878 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2983 3135 3170
Size: 274 80 33 51 1443 1006 152 35
Cuts at: 3170 4127 5839 96
Size: 957 1712 138
Fragments arranged by size:
1712 1443 1006 957 274 152 138 80
51 35 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2483 2515 5841 5873 2483
Size: 32 3326 32 2491
Fragments arranged by size:
3326 2491 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5881
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2290 2290
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 3206 1870
Size: 1336 4545
XmnI GAAnn'nnTTC
Cuts at: 2787 4010 2787
Size: 1223 4658
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI