Restriction map for
pFN21AE3165
(Circular) (Six-base) MAPSORT of: pFN21AE3165.seq Check: 9028 from: 1 to: 5866
pFN21AE3165 5866 bp
With 182 enzymes: SgfI *
March 28, 2012 13:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2785 3209 1937
Size: 848 424 4594
Fragments arranged by size:
4594 848 424
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2038 2586 2910 3929 5313 5581 1632
Size: 406 548 324 1019 1384 268 1917
Fragments arranged by size:
1917 1384 1019 548 406 324 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 2350 4595 2350
Size: 2245 3621
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2778 1184
Size: 1594 4272
AlwNI CAG_nnn'CTG
Cuts at: 2061 2094 3095 3609 5181 2061
Size: 33 1001 514 1572 2746
Fragments arranged by size:
2746 1572 1001 514 33
ApaLI G'TGCA_C
Cuts at: 3923 5079 3923
Size: 1156 4710
ApoI r'AATT_y
Cuts at: 1493 2693 2921 3175 3401 3553 5662 1493
Size: 1200 228 254 226 152 2109 1697
Fragments arranged by size:
2109 1697 1200 254 228 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 3191 1870
Size: 73 1248 4545
Fragments arranged by size:
4545 1248 73
BamHI G'GATC_C
Cuts at: 3196 3563 3196
Size: 367 5499
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3187 4642 618
Size: 325 217 627 1400 1455 1842
Fragments arranged by size:
1842 1455 1400 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3185 1391
Size: 1794 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2105 2328 2592 2955 2977 928
Size: 971 206 223 264 363 22 3817
Fragments arranged by size:
3817 971 363 264 223 206 22
BbvCI CC'TCA_GC
Cuts at: 2087 2087
Size: 5866
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3079 3758 4968 3079
Size: 679 1210 3977
Fragments arranged by size:
3977 1210 679
BclI T'GATC_A
Cuts at: 1548 2517 2803 1548
Size: 969 286 4611
Fragments arranged by size:
4611 969 286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2638 4477 136
Size: 107 122 71 631 605 966 1839 1525
Fragments arranged by size:
1839 1525 966 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2362 5861 2362
Size: 3499 2367
BlpI GC'TnA_GC
Cuts at: 3283 3283
Size: 5866
Bme1580I G_kGCm'C
Cuts at: 1790 3927 5083 1790
Size: 2137 1156 2573
Fragments arranged by size:
2573 2137 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2071 3597 4555
Size: 416 53 83 399 24 654 1526 958
Cuts at: 4555 442
Size: 1753
Fragments arranged by size:
1753 1526 958 654 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 4526 1239
Size: 854 2433 2579
Fragments arranged by size:
2579 2433 854
Bpu10I CC'TnA_GC
Cuts at: 2087 2087
Size: 5866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2985 3991 4856 5154 2985
Size: 1006 865 298 3697
Fragments arranged by size:
3697 1006 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4529 882
Size: 617 3030 2219
Fragments arranged by size:
3030 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 5860 5860
Size: 5866
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 4055 4690 275
Size: 53 83 186 936 2140 382 635 1451
Fragments arranged by size:
2140 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4299 4697 4971 5118 5529 4299
Size: 398 274 147 411 4636
Fragments arranged by size:
4636 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5641
Fragments arranged by size:
5641 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 1186
Size: 982 4884
BseYI C'CCAG_C
Cuts at: 5069 5565 5069
Size: 496 5370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2784 1163
Size: 573 1048 4245
Fragments arranged by size:
4245 1048 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 4077 5105 1540
Size: 275 186 1315 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3927 4012 5083 3927
Size: 85 1071 4710
Fragments arranged by size:
4710 1071 85
BsiWI C'GTAC_G
Cuts at: 2138 2138
Size: 5866
BsmI GAATG_Cn'
Cuts at: 3024 3377 3470 3024
Size: 353 93 5420
Fragments arranged by size:
5420 353 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3185 3927 4012 5083 1391
Size: 399 1395 742 85 1071 2174
Fragments arranged by size:
2174 1395 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 2233 3040 3755 5485 2233
Size: 807 715 1730 2614
Fragments arranged by size:
2614 1730 807 715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3221 844
Size: 2377 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3753 1394
Size: 429 1930 3507
Fragments arranged by size:
3507 1930 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2946 4361 4535 65
Size: 1183 1698 1415 174 1396
Fragments arranged by size:
1698 1415 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2817 4510 4697 1700
Size: 110 1007 1693 187 2869
Fragments arranged by size:
2869 1693 1007 187 110
BsrGI T'GTAC_A
Cuts at: 96 2200 96
Size: 2104 3762
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 3926 4938 3926
Size: 1012 4854
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5866
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5866
BstXI CCAn_nnnn'nTGG
Cuts at: 2298 2298
Size: 5866
BstYI r'GATC_y
Cuts at: 1972 2287 2320 2362 2947 3196 3563 3948 3965
Size: 315 33 42 585 249 367 385 17
Cuts at: 3965 5417 5503 5515 5861 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 585 385 367 346 315 249
86 42 33 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3071 513
Size: 552 939 1067 3308
Fragments arranged by size:
3308 1067 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 3382 4176 4196 3382
Size: 794 20 5052
Fragments arranged by size:
5052 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4873 809
Size: 507 3557 1802
Fragments arranged by size:
3557 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2169 3313
Size: 54 1425 50 275 18 249 90 1144
Cuts at: 3313 4202 5831 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1144 889 275 249 90 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 1537
Size: 275 1501 4090
Fragments arranged by size:
4090 1501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2361 2452 2745 3796 1353
Size: 1008 91 293 1051 3423
Fragments arranged by size:
3423 1051 1008 293 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4453 4827 4973 387
Size: 1064 138 2864 374 146 1280
Fragments arranged by size:
2864 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2093 2586 2910 3929 4526 5313
Size: 393 406 55 493 324 1019 597 787
Cuts at: 5313 5581 1239
Size: 268 1524
Fragments arranged by size:
1524 1019 787 597 493 406 393 324
268 55
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2478 2478
Size: 5866
FspI TGC'GCA
Cuts at: 4372 4372
Size: 5866
HaeII r_GCGC'y
Cuts at: 1678 5013 1678
Size: 3335 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2336 2368 2649 2681 2767
Size: 32 135 32 764 32 281 32 86
Cuts at: 2767 2799 4507 4539 4581 4613 1373
Size: 32 1708 32 42 32 2626
Fragments arranged by size:
2626 1708 764 281 135 86 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2344 2770 2786 3210 3456 669
Size: 988 281 406 426 16 424 246 3079
Fragments arranged by size:
3079 988 426 424 406 281 246 16
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 2770 3456 2770
Size: 686 5180
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4980 5164 1305
Size: 3675 184 2007
Fragments arranged by size:
3675 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4044
Fragments arranged by size:
4044 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2760 3824 4183 4342 518
Size: 648 1594 1064 359 159 2042
Fragments arranged by size:
2042 1594 1064 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2028 2091 2624 3282 3959 5107 5352 1606
Size: 422 63 533 658 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 658 533 422 245 63
NcoI C'CATG_G
Cuts at: 513 1065 2004 3071 513
Size: 552 939 1067 3308
Fragments arranged by size:
3308 1067 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 3224 4769 1132
Size: 97 1995 1545 2229
Fragments arranged by size:
2229 1995 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5866
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2624 2957 1108
Size: 1516 333 4017
Fragments arranged by size:
4017 1516 333
PfoI T'CCnGG_A
Cuts at: 3081 3081
Size: 5866
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PstI C_TGCA'G
Cuts at: 830 3218 830
Size: 2388 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
PvuII CAG'CTG
Cuts at: 2624 2624
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 2784 3208 1936
Size: 848 424 4594
Fragments arranged by size:
4594 848 424
SapI GCTCTTCn'nnn_
Cuts at: 2452 2745 2452
Size: 293 5573
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 4114 5826 4114
Size: 1712 4154
SexAI A'CCwGG_T
Cuts at: 2554 2554
Size: 5866
SfcI C'TryA_G
Cuts at: 826 1045 2431 3214 4349 5030 5221 826
Size: 219 1386 783 1135 681 191 1471
Fragments arranged by size:
1471 1386 1135 783 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 3193 1872
Size: 1321 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3000 3970 4871 5133 820
Size: 197 926 1057 970 901 262 1553
Fragments arranged by size:
1553 1057 970 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 3224 1132
Size: 2092 3774
SspI AAT'ATT
Cuts at: 5 52 3790 5
Size: 47 3738 2081
Fragments arranged by size:
3738 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5866
StyI C'CwwG_G
Cuts at: 513 1065 2004 3071 3305 513
Size: 552 939 1067 234 3074
Fragments arranged by size:
3074 1067 939 552 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3084 3893 3910 4063 4248 4693 325
Size: 1255 1504 809 17 153 185 445 1498
Fragments arranged by size:
1504 1498 1255 809 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2200 2255 4112
Size: 274 80 33 51 1443 223 55 1857
Cuts at: 4112 5824 96
Size: 1712 138
Fragments arranged by size:
1857 1712 1443 274 223 138 80 55
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5826 5858 5826
Size: 32 5834
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 2365 3202 2365
Size: 837 5029
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5866
XmaI C'CCGG_G
Cuts at: 1870 3191 1870
Size: 1321 4545
XmnI GAAnn'nnTTC
Cuts at: 2102 2461 3995 2102
Size: 359 1534 3973
Fragments arranged by size:
3973 1534 359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI
XcmI