Restriction map for
pFN21AE3164
(Circular) (Six-base) MAPSORT of: pFN21AE3164.seq Check: 7954 from: 1 to: 5860
pFN21AE3164 5860 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2997 3203 1937
Size: 1060 206 4594
Fragments arranged by size:
4594 1060 206
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 2549 3111 3923 5307 5575 1632
Size: 731 186 562 812 1384 268 1917
Fragments arranged by size:
1917 1384 812 731 562 268 186
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 4759 5736 1203
Size: 3556 977 1327
Fragments arranged by size:
3556 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2407 2439 2407
Size: 32 5828
AlwNI CAG_nnn'CTG
Cuts at: 3043 3603 5175 3043
Size: 560 1572 3728
Fragments arranged by size:
3728 1572 560
ApaI G_GGCC'C
Cuts at: 2644 2644
Size: 5860
ApaLI G'TGCA_C
Cuts at: 2701 2762 3917 5073 2701
Size: 61 1155 1156 3488
Fragments arranged by size:
3488 1156 1155 61
ApoI r'AATT_y
Cuts at: 1493 2583 3169 3395 3547 5656 1493
Size: 1090 586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1090 586 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 3185 1870
Size: 73 1242 4545
Fragments arranged by size:
4545 1242 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2918 3181 4636 618
Size: 325 217 627 1131 263 1455 1842
Fragments arranged by size:
1842 1455 1131 627 325 263 217
BanII G_rGCy'C
Cuts at: 1391 2644 3179 1391
Size: 1253 535 4072
Fragments arranged by size:
4072 1253 535
BbeI G_GCGC'C
Cuts at: 2922 2922
Size: 5860
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2113 2351 928
Size: 971 214 238 4437
Fragments arranged by size:
4437 971 238 214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4051 4085 1903
Size: 34 50 34 2030 34 3678
Fragments arranged by size:
3678 2030 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4471 136
Size: 107 122 71 631 605 2799 1525
Fragments arranged by size:
2799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2928 5855 2928
Size: 2927 2933
BlpI GC'TnA_GC
Cuts at: 3277 3277
Size: 5860
Bme1580I G_kGCm'C
Cuts at: 1790 2644 2705 2766 3921 5077 1790
Size: 854 61 61 1155 1156 2573
Fragments arranged by size:
2573 1156 1155 854 61 61
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2638 3591 4549
Size: 416 53 83 399 24 1221 953 958
Cuts at: 4549 442
Size: 1753
Fragments arranged by size:
1753 1221 958 953 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2447 1056
Size: 1391 4469
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2795 4520 1239
Size: 1556 1725 2579
Fragments arranged by size:
2579 1725 1556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2210 4523 882
Size: 617 711 2313 2219
Fragments arranged by size:
2313 2219 711 617
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 2496 5854 2496
Size: 3358 2502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2919 3667 4049 4684
Size: 53 83 186 936 1386 748 382 635
Cuts at: 4684 275
Size: 1451
Fragments arranged by size:
1451 1386 936 748 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4293 4691 4965 5112 5523 4293
Size: 398 274 147 411 4630
Fragments arranged by size:
4630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5635
Fragments arranged by size:
5635 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2999 1186
Size: 1813 4047
BseYI C'CCAG_C
Cuts at: 5063 5559 5063
Size: 496 5364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2131 1163
Size: 573 395 4892
Fragments arranged by size:
4892 573 395
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2142 3310 4071 5099 1540
Size: 275 186 141 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186 141
BsiHKAI G_wGCw'C
Cuts at: 2705 2766 3921 4006 5077 2705
Size: 61 1155 85 1071 3488
Fragments arranged by size:
3488 1155 1071 85 61
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2644 2705 2766 3179 3921 4006 5077
Size: 399 854 61 61 413 742 85 1071
Cuts at: 5077 1391
Size: 2174
Fragments arranged by size:
2174 1071 854 742 413 399 85 61
61
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 3028 3749 5479 3028
Size: 721 1730 3409
Fragments arranged by size:
3409 1730 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2456 3215 844
Size: 1612 759 3489
Fragments arranged by size:
3489 1612 759
BsrBI CCG'CTC
Cuts at: 1394 1823 2102 3747 1394
Size: 429 279 1645 3507
Fragments arranged by size:
3507 1645 429 279
BsrDI GCAATG_nn'
Cuts at: 65 1248 3053 4355 4529 65
Size: 1183 1805 1302 174 1396
Fragments arranged by size:
1805 1396 1302 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2086 2108 4504 4691 1700
Size: 110 276 22 2396 187 2869
Fragments arranged by size:
2869 2396 276 187 110 22
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 2065 1925
Size: 140 5720
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5860
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 2491 2901 2928 3190 3557 3942 3959 5411
Size: 519 410 27 262 367 385 17 1452
Cuts at: 5411 5497 5509 5855 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 519 410 385 367 346 262
86 27 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2136 2895 2947 3123 513
Size: 552 1071 759 52 176 3250
Fragments arranged by size:
3250 1071 759 552 176 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2827 487
Size: 683 56 1601 3520
Fragments arranged by size:
3520 1601 683 56
BtsI GCAGTG_nn'
Cuts at: 3376 4170 4190 3376
Size: 794 20 5046
Fragments arranged by size:
5046 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2835 2870 529
Size: 35 2271 35 3519
Fragments arranged by size:
3519 2271 35 35
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4867 809
Size: 507 3551 1802
Fragments arranged by size:
3551 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2127 2139
Size: 54 1425 50 275 18 258 39 12
Cuts at: 2139 2157 2260 2676 2793 2940 3307 4196 5825
Size: 18 103 416 117 147 367 889 1629
Cuts at: 5825 8
Size: 43
Fragments arranged by size:
1629 1425 889 416 367 275 258 147
117 103 54 50 43 39 18 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 2139 3307 1537
Size: 275 327 1168 4090
Fragments arranged by size:
4090 1168 327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 3790 1353
Size: 1058 1379 3423
Fragments arranged by size:
3423 1379 1058
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2549 2795 3111 3923 4520 5307
Size: 393 731 186 246 316 812 597 787
Cuts at: 5307 5575 1239
Size: 268 1524
Fragments arranged by size:
1524 812 787 731 597 393 316 268
246 186
EcoNI CCTnn'n_nnAGG
Cuts at: 2356 2981 2356
Size: 625 5235
EcoO109I rG'GnC_Cy
Cuts at: 2640 2968 2640
Size: 328 5532
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
EcoRV GAT'ATC
Cuts at: 3073 3073
Size: 5860
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2179 2922 5007 1678
Size: 501 743 2085 2531
Fragments arranged by size:
2531 2085 743 501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2650 2682 2826 2858 4501
Size: 32 135 32 1078 32 144 32 1643
Cuts at: 4501 4533 4575 4607 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1643 1078 144 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KasI G'GCGC_C
Cuts at: 2918 2918
Size: 5860
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2803 2812 4974 5158 1305
Size: 1498 9 2162 184 2007
Fragments arranged by size:
2162 2007 1498 184 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2159 2262 2795 2942 10
Size: 54 1425 343 327 103 533 147 2928
Fragments arranged by size:
2928 1425 533 343 327 147 103 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3149 3818 4177 4336 518
Size: 648 1983 669 359 159 2042
Fragments arranged by size:
2042 1983 669 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2309 2897 3276 3953 5101 5346 1606
Size: 703 588 379 677 1148 245 2120
Fragments arranged by size:
2120 1148 703 677 588 379 245
NaeI GCC'GGC
Cuts at: 2088 2088
Size: 5860
NarI GG'CG_CC
Cuts at: 2919 2919
Size: 5860
NcoI C'CATG_G
Cuts at: 513 1065 3123 513
Size: 552 2058 3250
Fragments arranged by size:
3250 2058 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NgoMIV G'CCGG_C
Cuts at: 2086 2086
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 2443 1052
Size: 1391 4469
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 3218 4763 1132
Size: 97 1989 1545 2229
Fragments arranged by size:
2229 1989 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 4759 4759
Size: 5860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5860
PfoI T'CCnGG_A
Cuts at: 2574 2574
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2724 1613
Size: 1111 4749
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PspOMI G'GGCC_C
Cuts at: 2640 2640
Size: 5860
PstI C_TGCA'G
Cuts at: 830 2965 3212 830
Size: 2135 247 3478
Fragments arranged by size:
3478 2135 247
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2309 2309
Size: 5860
SacII CC_GC'GG
Cuts at: 2898 2898
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2392 2817 2961 3208 4343 5024 5215
Size: 219 1347 425 144 247 1135 681 191
Cuts at: 5215 826
Size: 1471
Fragments arranged by size:
1471 1347 1135 681 425 247 219 191
144
SfoI GGC'GCC
Cuts at: 2920 2920
Size: 5860
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3964 4865 5127 820
Size: 197 926 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 2772 1517
Size: 1255 4605
StyI C'CwwG_G
Cuts at: 513 1065 2179 3123 3299 513
Size: 552 1114 944 176 3074
Fragments arranged by size:
3074 1114 944 552 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2781 2960 3887 3904 4057 4242 4687
Size: 1255 1201 179 927 17 153 185 445
Cuts at: 4687 325
Size: 1498
Fragments arranged by size:
1498 1255 1201 927 445 185 179 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4106 5818 96
Size: 274 80 33 51 1443 2129 1712 138
Fragments arranged by size:
2129 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2684 2716 5820 5852 2684
Size: 32 3104 32 2692
Fragments arranged by size:
3104 2692 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2664 1316
Size: 1348 4512
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 2253 2583 3096 3989 2253
Size: 330 513 893 4124
Fragments arranged by size:
4124 893 513 330
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI
SwaI XcmI