Restriction map for
pFN21AE3161
(Circular) (Six-base) MAPSORT of: pFN21AE3161.seq Check: 9563 from: 1 to: 5830
pFN21AE3161 5830 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2724 3049 3173 1937
Size: 787 325 124 4594
Fragments arranged by size:
4594 787 325 124
Acc65I G'GTAC_C
Cuts at: 2954 3151 2954
Size: 197 5633
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2154 2168 2386 3893 5277 5545 1632
Size: 522 14 218 1507 1384 268 1917
Fragments arranged by size:
1917 1507 1384 522 268 218 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 3127 4729 5706 1203
Size: 1924 1602 977 1327
Fragments arranged by size:
1924 1602 1327 977
AgeI A'CCGG_T
Cuts at: 2061 4661 2061
Size: 2600 3230
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AlwNI CAG_nnn'CTG
Cuts at: 3573 5145 3573
Size: 1572 4258
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 2851 2928 3139 3365 3517 5626 1493
Size: 1358 77 211 226 152 2109 1697
Fragments arranged by size:
2109 1697 1358 226 211 152 77
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 3155 1870
Size: 73 1212 4545
Fragments arranged by size:
4545 1212 73
BamHI G'GATC_C
Cuts at: 2827 3160 3527 2827
Size: 333 367 5130
Fragments arranged by size:
5130 367 333
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2954 3151 4606 618
Size: 325 217 627 1167 197 1455 1842
Fragments arranged by size:
1842 1455 1167 627 325 217 197
BanII G_rGCy'C
Cuts at: 1391 2549 3149 1391
Size: 1158 600 4072
Fragments arranged by size:
4072 1158 600
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2639 928
Size: 971 740 4119
Fragments arranged by size:
4119 971 740
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2011 2045 4021 4055 1903
Size: 34 74 34 1976 34 3678
Fragments arranged by size:
3678 1976 74 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2192 2386 2487 3722 4932 2192
Size: 194 101 1235 1210 3090
Fragments arranged by size:
3090 1235 1210 194 101
BclI T'GATC_A
Cuts at: 1548 2965 1548
Size: 1417 4413
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4441 136
Size: 107 122 71 631 605 2769 1525
Fragments arranged by size:
2769 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 2804 3247 2804
Size: 443 5387
Bme1580I G_kGCm'C
Cuts at: 1790 2042 3891 5047 1790
Size: 252 1849 1156 2573
Fragments arranged by size:
2573 1849 1156 252
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2323 2980 3561
Size: 416 53 83 399 24 906 657 581
Cuts at: 3561 4519 442
Size: 958 1753
Fragments arranged by size:
1753 958 906 657 581 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4490 1239
Size: 3251 2579
Bpu10I CC'TnA_GC
Cuts at: 2097 2097
Size: 5830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2323 2444 2666 3955 4820 5118 2323
Size: 121 222 1289 865 298 3035
Fragments arranged by size:
3035 1289 865 298 222 121
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4493 882
Size: 617 2994 2219
Fragments arranged by size:
2994 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3637 4019 4654 275
Size: 53 83 186 936 2104 382 635 1451
Fragments arranged by size:
2104 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2061 4263 4661 4935 5082 5493 2061
Size: 2202 398 274 147 411 2398
Fragments arranged by size:
2398 2202 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5605
Fragments arranged by size:
5605 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2310 2313 1186
Size: 1124 3 4703
Fragments arranged by size:
4703 1124 3
BseYI C'CCAG_C
Cuts at: 5033 5529 5033
Size: 496 5334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2066 3280 4041 5069 1540
Size: 275 186 65 1214 761 1028 2301
Fragments arranged by size:
2301 1214 1028 761 275 186 65
BsiHKAI G_wGCw'C
Cuts at: 2549 3891 3976 5047 2549
Size: 1342 85 1071 3332
Fragments arranged by size:
3332 1342 1071 85
BsmI GAATG_Cn'
Cuts at: 2158 2229 2600 3341 3434 2158
Size: 71 371 741 93 4554
Fragments arranged by size:
4554 741 371 93 71
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2549 3149 3891 3976 5047 1391
Size: 399 252 507 600 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 600 507 399 252 85
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2405 3185 844
Size: 1561 780 3489
Fragments arranged by size:
3489 1561 780
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2061 4474 4661 1700
Size: 110 251 2413 187 2869
Fragments arranged by size:
2869 2413 251 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 2886 3890 4902 2886
Size: 1004 1012 3814
Fragments arranged by size:
3814 1012 1004
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2429 794
Size: 1635 4195
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 2827 3160 3527 3912 3929 5381 5467 5479
Size: 855 333 367 385 17 1452 86 12
Cuts at: 5479 5825 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 855 385 367 346 333 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2117 2117
Size: 5830
BtgI C'CryG_G
Cuts at: 513 1065 2004 2663 513
Size: 552 939 659 3680
Fragments arranged by size:
3680 939 659 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 3346 4140 4160 3346
Size: 794 20 5016
Fragments arranged by size:
5016 794 20
ClaI AT'CG_AT
Cuts at: 1554 2625 1554
Size: 1071 4759
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DraIII CAC_nnn'GTG
Cuts at: 2669 2669
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3277 4166 5795
Size: 54 1425 50 275 18 1447 889 1629
Cuts at: 5795 8
Size: 43
Fragments arranged by size:
1629 1447 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2098 2346 3760 1353
Size: 745 248 1414 3423
Fragments arranged by size:
3423 1414 745 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2547 2547
Size: 5830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2168 2386 3893 4490 5277 5545
Size: 393 522 14 218 1507 597 787 268
Cuts at: 5545 1239
Size: 1524
Fragments arranged by size:
1524 1507 787 597 522 393 268 218
14
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 4977 1678
Size: 3299 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2347 2379 4471 4503 4545
Size: 32 135 32 775 32 2092 32 42
Cuts at: 4545 4577 1373
Size: 32 2626
Fragments arranged by size:
2626 2092 775 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 2958 3155 2958
Size: 197 5633
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4944 5128 1305
Size: 3639 184 2007
Fragments arranged by size:
3639 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4008
Fragments arranged by size:
4008 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3788 4147 4306 518
Size: 648 843 1779 359 159 2042
Fragments arranged by size:
2042 1779 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2179 3246 3923 5071 5316 1606
Size: 573 1067 677 1148 245 2120
Fragments arranged by size:
2120 1148 1067 677 573 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4339
Fragments arranged by size:
4339 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 3188 4733 1132
Size: 97 1959 1545 2229
Fragments arranged by size:
2229 1959 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2791 1348
Size: 1443 4387
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5830
PfoI T'CCnGG_A
Cuts at: 2531 2895 2531
Size: 364 5466
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PstI C_TGCA'G
Cuts at: 830 2485 3182 830
Size: 1655 697 3478
Fragments arranged by size:
3478 1655 697
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
PvuII CAG'CTG
Cuts at: 2179 2179
Size: 5830
SacI G_AGCT'C
Cuts at: 2549 2549
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2481 3178 4313 4994 5185 826
Size: 219 1436 697 1135 681 191 1471
Fragments arranged by size:
1471 1436 1135 697 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2338 2459 2645 3934 4835 5097
Size: 197 926 395 121 186 1289 901 262
Cuts at: 5097 820
Size: 1553
Fragments arranged by size:
1553 1289 926 901 395 262 197 186
121
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 3044 152
Size: 2892 2938
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5830
StyI C'CwwG_G
Cuts at: 513 1065 2004 3269 513
Size: 552 939 1265 3074
Fragments arranged by size:
3074 1265 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3857 3874 4027 4212 4657 325
Size: 1255 2277 17 153 185 445 1498
Fragments arranged by size:
2277 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2212 4076 5788
Size: 274 80 33 51 1443 235 1864 1712
Cuts at: 5788 96
Size: 138
Fragments arranged by size:
1864 1712 1443 274 235 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5790 5822 5790
Size: 32 5798
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI