Restriction map for
pFN21AE3159
(Circular) (Six-base) MAPSORT of: pFN21AE3159.seq Check: 6112 from: 1 to: 5839
pFN21AE3159 5839 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3182 1937
Size: 1245 4594
Acc65I G'GTAC_C
Cuts at: 3011 3160 3011
Size: 149 5690
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2388 2475 3902 5286 5554 1632
Size: 756 87 1427 1384 268 1917
Fragments arranged by size:
1917 1427 1384 756 268 87
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 2142 4738 5715 1203
Size: 939 2596 977 1327
Fragments arranged by size:
2596 1327 977 939
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 2516 3582 5154 2516
Size: 1066 1572 3201
Fragments arranged by size:
3201 1572 1066
ApaLI G'TGCA_C
Cuts at: 3896 5052 3896
Size: 1156 4683
ApoI r'AATT_y
Cuts at: 1493 2023 2333 2343 2989 3148 3374 3526 5635
Size: 530 310 10 646 159 226 152 2109
Cuts at: 5635 1493
Size: 1697
Fragments arranged by size:
2109 1697 646 530 310 226 159 152
10
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 3164 1870
Size: 73 1221 4545
Fragments arranged by size:
4545 1221 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2056 2089 2056
Size: 33 5806
BamHI G'GATC_C
Cuts at: 2811 3169 3536 2811
Size: 358 367 5114
Fragments arranged by size:
5114 367 358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 3011 3160 4615 618
Size: 325 217 627 266 958 149 1455 1842
Fragments arranged by size:
1842 1455 958 627 325 266 217 149
BanII G_rGCy'C
Cuts at: 1391 2718 3158 1391
Size: 1327 440 4072
Fragments arranged by size:
4072 1327 440
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4868
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4030 4064 1903
Size: 34 2093 34 3678
Fragments arranged by size:
3678 2093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2595 3731 4941 2595
Size: 1136 1210 3493
Fragments arranged by size:
3493 1210 1136
BclI T'GATC_A
Cuts at: 1548 2356 1548
Size: 808 5031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4450 136
Size: 107 122 71 631 605 2778 1525
Fragments arranged by size:
2778 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5834 5834
Size: 5839
BlpI GC'TnA_GC
Cuts at: 3256 3256
Size: 5839
Bme1580I G_kGCm'C
Cuts at: 1790 3900 5056 1790
Size: 2110 1156 2573
Fragments arranged by size:
2573 2110 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2078 2278 2743
Size: 416 53 83 399 24 661 200 465
Cuts at: 2743 3570 4528 442
Size: 827 958 1753
Fragments arranged by size:
1753 958 827 661 465 416 399 200
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2652 2724 4499 1239
Size: 1413 72 1775 2579
Fragments arranged by size:
2579 1775 1413 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2413 3964 4829 5127 2413
Size: 1551 865 298 3125
Fragments arranged by size:
3125 1551 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4502 882
Size: 617 3003 2219
Fragments arranged by size:
3003 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3630 493
Size: 711 2426 2702
Fragments arranged by size:
2702 2426 711
BsaBI GATnn'nnATC
Cuts at: 5833 5833
Size: 5839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3646 4028 4663 275
Size: 53 83 186 936 2113 382 635 1451
Fragments arranged by size:
2113 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4272 4670 4944 5091 5502 4272
Size: 398 274 147 411 4609
Fragments arranged by size:
4609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5614
Fragments arranged by size:
5614 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3018 1186
Size: 1832 4007
BseYI C'CCAG_C
Cuts at: 2119 5042 5538 2119
Size: 2923 496 2420
Fragments arranged by size:
2923 2420 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3289 4050 5078 1540
Size: 275 186 1288 761 1028 2301
Fragments arranged by size:
2301 1288 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2718 2844 3900 3985 5056 2718
Size: 126 1056 85 1071 3501
Fragments arranged by size:
3501 1071 1056 126 85
BsmI GAATG_Cn'
Cuts at: 2439 2919 3350 3443 2439
Size: 480 431 93 4835
Fragments arranged by size:
4835 480 431 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2718 2844 3158 3900 3985 5056 1391
Size: 399 928 126 314 742 85 1071 2174
Fragments arranged by size:
2174 1071 928 742 399 314 126 85
BspEI T'CCGG_A
Cuts at: 5502 5502
Size: 5839
BspHI T'CATG_A
Cuts at: 2767 3728 5458 2767
Size: 961 1730 3148
Fragments arranged by size:
3148 1730 961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3194 844
Size: 2350 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3726 1394
Size: 429 1903 3507
Fragments arranged by size:
3507 1903 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2593 4334 4508 65
Size: 1183 1345 1741 174 1396
Fragments arranged by size:
1741 1396 1345 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4483 4670 1700
Size: 110 2673 187 2869
Fragments arranged by size:
2869 2673 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5839
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5839
BssSI C'ACGA_G
Cuts at: 3899 4911 3899
Size: 1012 4827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5839
BstBI TT'CG_AA
Cuts at: 4643 4643
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1184 2075 1184
Size: 891 4948
BstYI r'GATC_y
Cuts at: 1972 2811 3169 3536 3921 3938 5390 5476 5488
Size: 839 358 367 385 17 1452 86 12
Cuts at: 5488 5834 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 839 385 367 358 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5100
Fragments arranged by size:
5100 683 56
BtsI GCAGTG_nn'
Cuts at: 2165 3355 4149 4169 2165
Size: 1190 794 20 3835
Fragments arranged by size:
3835 1190 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 2965 3143 2965
Size: 178 5661
DraIII CAC_nnn'GTG
Cuts at: 2086 2086
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3286 4175 5804
Size: 54 1425 50 275 18 1456 889 1629
Cuts at: 5804 8
Size: 43
Fragments arranged by size:
1629 1456 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3286 1537
Size: 275 1474 4090
Fragments arranged by size:
4090 1474 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2226 2364 2851 3769 1353
Size: 873 138 487 918 3423
Fragments arranged by size:
3423 918 873 487 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4426 4800 4946 387
Size: 1064 138 2837 374 146 1280
Fragments arranged by size:
2837 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2716 2716
Size: 5839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2475 2652 2724 3902 4499 5286
Size: 393 756 87 177 72 1178 597 787
Cuts at: 5286 5554 1239
Size: 268 1524
Fragments arranged by size:
1524 1178 787 756 597 393 268 177
87 72
EcoO109I rG'GnC_Cy
Cuts at: 2555 2555
Size: 5839
EcoRI G'AATT_C
Cuts at: 3148 3526 3148
Size: 378 5461
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 4986 1678
Size: 3308 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4480 4512 4554 4586 1373
Size: 32 135 32 2908 32 42 32 2626
Fragments arranged by size:
2908 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3429 669
Size: 988 281 1245 246 3079
Fragments arranged by size:
3079 1245 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5839
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3015 3164 3015
Size: 149 5690
MfeI C'AATT_G
Cuts at: 3438 3438
Size: 5839
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2550 4953 5137 1305
Size: 1245 2403 184 2007
Fragments arranged by size:
2403 2007 1245 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4017
Fragments arranged by size:
4017 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3797 4156 4315 518
Size: 648 843 1788 359 159 2042
Fragments arranged by size:
2042 1788 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2213 3255 3932 5080 5325 1606
Size: 607 1042 677 1148 245 2120
Fragments arranged by size:
2120 1148 1042 677 607 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5287
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 2146 2158 3197 4742 1132
Size: 97 917 12 1039 1545 2229
Fragments arranged by size:
2229 1545 1039 917 97 12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 2142 4738 2142
Size: 2596 3243
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2086 1108
Size: 978 4861
PfoI T'CCnGG_A
Cuts at: 2814 2814
Size: 5839
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2555 2555
Size: 5839
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 3409 3409
Size: 5839
PstI C_TGCA'G
Cuts at: 830 3191 830
Size: 2361 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
PvuII CAG'CTG
Cuts at: 2213 2213
Size: 5839
SacI G_AGCT'C
Cuts at: 2718 2718
Size: 5839
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SapI GCTCTTCn'nnn_
Cuts at: 2226 2226
Size: 5839
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 4087 5799 4087
Size: 1712 4127
SfcI C'TryA_G
Cuts at: 826 1045 2737 3187 4322 5003 5194 826
Size: 219 1692 450 1135 681 191 1471
Fragments arranged by size:
1692 1471 1135 681 450 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 3166 1872
Size: 1294 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2428 3943 4844 5106 820
Size: 197 926 485 1515 901 262 1553
Fragments arranged by size:
1553 1515 926 901 485 262 197
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 2408 152
Size: 2256 3583
SphI G_CATG'C
Cuts at: 1132 3197 1132
Size: 2065 3774
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5839
StyI C'CwwG_G
Cuts at: 513 1065 3278 513
Size: 552 2213 3074
Fragments arranged by size:
3074 2213 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2097 3866 3883 4036 4221 4666 325
Size: 1255 517 1769 17 153 185 445 1498
Fragments arranged by size:
1769 1498 1255 517 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4085 5797 96
Size: 274 80 33 51 1443 2108 1712 138
Fragments arranged by size:
2108 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2619 2651 2857 2889 5799 5831 2619
Size: 32 206 32 2910 32 2627
Fragments arranged by size:
2910 2627 206 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5839
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 3164 1870
Size: 1294 4545
XmnI GAAnn'nnTTC
Cuts at: 2346 3968 2346
Size: 1622 4217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI