Restriction map for
pFN21AE3158
(Circular) (Six-base) MAPSORT of: pFN21AE3158.seq Check: 1282 from: 1 to: 5833
pFN21AE3158 5833 bp
With 182 enzymes: SgfI *
March 28, 2012 12:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3176 1937
Size: 1239 4594
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 2397 3960 4333 1563
Size: 834 1563 373 3063
Fragments arranged by size:
3063 1563 834 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2678 3896 5280 5548 1632
Size: 1046 1218 1384 268 1917
Fragments arranged by size:
1917 1384 1218 1046 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5636
AflIII A'CryG_T
Cuts at: 1203 4732 5709 1203
Size: 3529 977 1327
Fragments arranged by size:
3529 1327 977
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AlwNI CAG_nnn'CTG
Cuts at: 2408 3576 5148 2408
Size: 1168 1572 3093
Fragments arranged by size:
3093 1572 1168
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 2490 3142 3368 3520 5629 1493
Size: 997 652 226 152 2109 1697
Fragments arranged by size:
2109 1697 997 652 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 1870
Size: 73 1215 4545
Fragments arranged by size:
4545 1215 73
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3154 4609 618
Size: 325 217 627 1367 1455 1842
Fragments arranged by size:
1842 1455 1367 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3152 1391
Size: 1761 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4862
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4024 4058 1903
Size: 34 50 34 2003 34 3678
Fragments arranged by size:
3678 2003 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 2385 1548
Size: 837 4996
BfrBI ATG'CAT
Cuts at: 2008 2258 2008
Size: 250 5583
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2689 5828 2689
Size: 3139 2694
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 3894 5050 1790
Size: 2104 1156 2573
Fragments arranged by size:
2573 2104 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5833
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2202 4493 1239
Size: 963 2291 2579
Fragments arranged by size:
2579 2291 963
Bpu10I CC'TnA_GC
Cuts at: 2608 2608
Size: 5833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2000 3958 4823 5121 2000
Size: 1958 865 298 2712
Fragments arranged by size:
2712 1958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2294 2584 4496 882
Size: 617 795 290 1912 2219
Fragments arranged by size:
2219 1912 795 617 290
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 3120 5827 3120
Size: 2707 3126
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3640 4022 4657 275
Size: 53 83 186 936 2107 382 635 1451
Fragments arranged by size:
2107 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2664 4266 4664 4938 5085 5496 2664
Size: 1602 398 274 147 411 3001
Fragments arranged by size:
3001 1602 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2214 2244 1178
Size: 30 165 30 811 30 4767
Fragments arranged by size:
4767 811 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3020 3077 1186
Size: 1834 57 3942
Fragments arranged by size:
3942 1834 57
BseYI C'CCAG_C
Cuts at: 5036 5532 5036
Size: 496 5337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3283 4044 5072 1540
Size: 275 186 1282 761 1028 2301
Fragments arranged by size:
2301 1282 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3894 3979 5050 3894
Size: 85 1071 4677
Fragments arranged by size:
4677 1071 85
BsmI GAATG_Cn'
Cuts at: 3344 3437 3344
Size: 93 5740
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3152 3894 3979 5050 1391
Size: 399 1362 742 85 1071 2174
Fragments arranged by size:
2174 1362 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3722 5452 3722
Size: 1730 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3188 844
Size: 2344 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4477 4664 1700
Size: 110 2667 187 2869
Fragments arranged by size:
2869 2667 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5833
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 2689 3163 3530 3915 3932 5384 5470 5482
Size: 717 474 367 385 17 1452 86 12
Cuts at: 5482 5828 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 717 474 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2529 2872 513
Size: 552 1464 343 3474
Fragments arranged by size:
3474 1464 552 343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 2040 3349 4143 4163 2040
Size: 1309 794 20 3710
Fragments arranged by size:
3710 1309 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2828 2863 529
Size: 35 2264 35 3499
Fragments arranged by size:
3499 2264 35 35
DraI TTT'AAA
Cuts at: 3137 3137
Size: 5833
DraIII CAC_nnn'GTG
Cuts at: 2999 2999
Size: 5833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3280 4169 5798
Size: 54 1425 50 275 18 1450 889 1629
Cuts at: 5798 8
Size: 43
Fragments arranged by size:
1629 1450 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2053 2204 2997 3763 1353
Size: 700 151 793 766 3423
Fragments arranged by size:
3423 793 766 700 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2202 2678 3896 4493 5280 5548 1239
Size: 393 570 476 1218 597 787 268 1524
Fragments arranged by size:
1524 1218 787 597 570 476 393 268
EcoRI G'AATT_C
Cuts at: 3142 3520 3142
Size: 378 5455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2220 2220
Size: 5833
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 4980 1678
Size: 3302 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2373 2405 4474 4506 4548
Size: 32 135 32 801 32 2069 32 42
Cuts at: 4548 4580 1373
Size: 32 2626
Fragments arranged by size:
2626 2069 801 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 3432 3432
Size: 5833
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2740 4947 5131 1305
Size: 1435 2207 184 2007
Fragments arranged by size:
2207 2007 1435 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2561 3791 4150 4309 518
Size: 648 1395 1230 359 159 2042
Fragments arranged by size:
2042 1395 1230 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3249 3926 5074 5319 1606
Size: 1643 677 1148 245 2120
Fragments arranged by size:
2120 1643 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2872 513
Size: 552 1807 3474
Fragments arranged by size:
3474 1807 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NsiI A_TGCA'T
Cuts at: 2010 2260 2010
Size: 250 5583
NspI r_CATG'y
Cuts at: 1132 1229 2262 2883 3191 4736 1132
Size: 97 1033 621 308 1545 2229
Fragments arranged by size:
2229 1545 1033 621 308 97
PacI TTA_AT'TAA
Cuts at: 2505 2505
Size: 5833
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2408 1108
Size: 1300 4533
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 5415 5447 3902
Size: 32 1481 32 4288
Fragments arranged by size:
4288 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2183 1613
Size: 570 5263
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PstI C_TGCA'G
Cuts at: 830 3034 3185 830
Size: 2204 151 3478
Fragments arranged by size:
3478 2204 151
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3034 3185 3034
Size: 151 5682
ScaI AGT'ACT
Cuts at: 2294 4081 5793 2294
Size: 1787 1712 2334
Fragments arranged by size:
2334 1787 1712
SexAI A'CCwGG_T
Cuts at: 2415 2415
Size: 5833
SfcI C'TryA_G
Cuts at: 826 1045 3030 3181 4316 4997 5188 826
Size: 219 1985 151 1135 681 191 1471
Fragments arranged by size:
1985 1471 1135 681 219 191 151
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 3937 4838 5100 820
Size: 197 926 72 1922 901 262 1553
Fragments arranged by size:
1922 1553 926 901 262 197 72
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 3191 1132
Size: 2059 3774
SspI AAT'ATT
Cuts at: 5 52 3757 5
Size: 47 3705 2081
Fragments arranged by size:
3705 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2872 3272 513
Size: 552 1807 400 3074
Fragments arranged by size:
3074 1807 552 400
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3860 3877 4030 4215 4660 325
Size: 1255 2280 17 153 185 445 1498
Fragments arranged by size:
2280 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2292 4079 5791
Size: 274 80 33 51 1443 315 1787 1712
Cuts at: 5791 96
Size: 138
Fragments arranged by size:
1787 1712 1443 315 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2214 2246 5793 5825 2214
Size: 32 3547 32 2222
Fragments arranged by size:
3547 2222 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 3117 3962 3117
Size: 845 4988
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PacI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI