Restriction map for
pFN21AE3157
(Circular) (Six-base) MAPSORT of: pFN21AE3157.seq Check: 6610 from: 1 to: 5830
pFN21AE3157 5830 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 2558 3893 5277 5545 1632
Size: 456 470 1335 1384 268 1917
Fragments arranged by size:
1917 1384 1335 470 456 268
AfeI AGC'GCT
Cuts at: 2759 2759
Size: 5830
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 2649 1166
Size: 1483 4347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2778 2883 1184
Size: 1594 105 4131
Fragments arranged by size:
4131 1594 105
AlwNI CAG_nnn'CTG
Cuts at: 2605 2803 3573 5145 2605
Size: 198 770 1572 3290
Fragments arranged by size:
3290 1572 770 198
ApaI G_GGCC'C
Cuts at: 2320 2320
Size: 5830
ApaLI G'TGCA_C
Cuts at: 2402 3887 5043 2402
Size: 1485 1156 3189
Fragments arranged by size:
3189 1485 1156
ApoI r'AATT_y
Cuts at: 1493 3139 3365 3517 5626 1493
Size: 1646 226 152 2109 1697
Fragments arranged by size:
2109 1697 1646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 2364 3042 3155 1870
Size: 73 421 678 113 4545
Fragments arranged by size:
4545 678 421 113 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2279 2613 2851 3151 4606
Size: 325 217 627 492 334 238 300 1455
Cuts at: 4606 618
Size: 1842
Fragments arranged by size:
1842 1455 627 492 334 325 300 238
217
BanII G_rGCy'C
Cuts at: 1391 2320 2510 2577 2778 3149 1391
Size: 929 190 67 201 371 4072
Fragments arranged by size:
4072 929 371 201 190 67
BbeI G_GCGC'C
Cuts at: 2617 2855 2617
Size: 238 5592
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2543 3110 928
Size: 971 644 567 3648
Fragments arranged by size:
3648 971 644 567
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4021 4055 1903
Size: 34 2084 34 3678
Fragments arranged by size:
3678 2084 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2234 3722 4932 2234
Size: 1488 1210 3132
Fragments arranged by size:
3132 1488 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BfrBI ATG'CAT
Cuts at: 2860 2860
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2673 4441 136
Size: 107 122 71 631 605 1001 1768 1525
Fragments arranged by size:
1768 1525 1001 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 2504 2766 3247 2504
Size: 262 481 5087
Fragments arranged by size:
5087 481 262
Bme1580I G_kGCm'C
Cuts at: 1790 2282 2308 2320 2406 3891 5047 1790
Size: 492 26 12 86 1485 1156 2573
Fragments arranged by size:
2573 1485 1156 492 86 26 12
BmgBI CAC'GTC
Cuts at: 1318 2269 2407 2740 1318
Size: 951 138 333 4408
Fragments arranged by size:
4408 951 333 138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 3561 4519
Size: 416 53 83 399 24 828 1316 958
Cuts at: 4519 442
Size: 1753
Fragments arranged by size:
1753 1316 958 828 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2411 2647 2690 2825 2916 4490 1239
Size: 884 288 236 43 135 91 1574 2579
Fragments arranged by size:
2579 1574 884 288 236 135 91 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 3955 4820 5118 2051
Size: 1904 865 298 2763
Fragments arranged by size:
2763 1904 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3084 4493 882
Size: 617 1585 1409 2219
Fragments arranged by size:
2219 1585 1409 617
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2614 2852 3637 4019
Size: 53 83 186 936 1081 238 785 382
Cuts at: 4019 4654 275
Size: 635 1451
Fragments arranged by size:
1451 1081 936 785 635 382 238 186
83 53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2952 2982 1178
Size: 30 165 30 1549 30 4026
Fragments arranged by size:
4026 1549 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2960 3113 1186
Size: 1006 768 153 3903
Fragments arranged by size:
3903 1006 768 153
BseYI C'CCAG_C
Cuts at: 5033 5529 5033
Size: 496 5334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2474 2738 1163
Size: 573 738 264 4255
Fragments arranged by size:
4255 738 573 264
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2753 3280 4041 5069 1540
Size: 275 186 752 527 761 1028 2301
Fragments arranged by size:
2301 1028 761 752 527 275 186
BsiHKAI G_wGCw'C
Cuts at: 2406 2510 2577 2778 3891 3976 5047 2406
Size: 104 67 201 1113 85 1071 3189
Fragments arranged by size:
3189 1113 1071 201 104 85 67
BsmI GAATG_Cn'
Cuts at: 2286 3341 3434 2286
Size: 1055 93 4682
Fragments arranged by size:
4682 1055 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2282 2308 2320 2406 2510 2577 2778
Size: 399 492 26 12 86 104 67 201
Cuts at: 2778 3149 3891 3976 5047 1391
Size: 371 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 399 371 201 104
86 85 67 26 12
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3185 844
Size: 2341 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2089 3717 1394
Size: 429 266 1628 3507
Fragments arranged by size:
3507 1628 429 266
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2282 2560 2786 2986 4474 4661 1700
Size: 110 472 278 226 200 1488 187 2869
Fragments arranged by size:
2869 1488 472 278 226 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 2174 2492 3890 4902 2174
Size: 318 1398 1012 3102
Fragments arranged by size:
3102 1398 1012 318
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2410 794
Size: 1616 4214
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 2884 1184
Size: 1700 4130
BstYI r'GATC_y
Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825
Size: 1188 367 385 17 1452 86 12 346
Cuts at: 5825 1972
Size: 1977
Fragments arranged by size:
1977 1452 1188 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3120 513
Size: 552 2055 3223
Fragments arranged by size:
3223 2055 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 2243 3346 4140 4160 2243
Size: 1103 794 20 3913
Fragments arranged by size:
3913 1103 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2168 4837 809
Size: 507 852 2669 1802
Fragments arranged by size:
2669 1802 852 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2750 2946 3277
Size: 54 1425 50 275 18 920 196 331
Cuts at: 3277 4166 5795 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 920 889 331 275 196 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2750 3277 1537
Size: 275 938 527 4090
Fragments arranged by size:
4090 938 527 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 2582 3760 1353
Size: 711 518 1178 3423
Fragments arranged by size:
3423 1178 711 518
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2557 4417 4791 4937 387
Size: 1064 138 968 1860 374 146 1280
Fragments arranged by size:
1860 1280 1064 968 374 146 138
EcoICRI GAG'CTC
Cuts at: 2508 2575 2776 2508
Size: 67 201 5562
Fragments arranged by size:
5562 201 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2123 2411 2558 2647 2690 2825
Size: 393 456 35 288 147 89 43 135
Cuts at: 2825 2916 3893 4490 5277 5545 1239
Size: 91 977 597 787 268 1524
Fragments arranged by size:
1524 977 787 597 456 393 288 268
147 135 91 89 43 35
EcoO109I rG'GnC_Cy
Cuts at: 2316 2316
Size: 5830
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 2349 2617 2761 2827 2855 2969 4977 1678
Size: 671 268 144 66 28 114 2008 2531
Fragments arranged by size:
2531 2008 671 268 144 114 66 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373
Size: 32 135 32 2899 32 42 32 2626
Fragments arranged by size:
2899 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3174 3420 669
Size: 988 281 1236 246 3079
Fragments arranged by size:
3079 1236 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2027 748
Size: 1279 4551
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KasI G'GCGC_C
Cuts at: 2613 2851 2613
Size: 238 5592
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2886 4944 5128 1305
Size: 1581 2058 184 2007
Fragments arranged by size:
2058 2007 1581 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4008
Fragments arranged by size:
4008 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2649 3788 4147 4306 518
Size: 648 1483 1139 359 159 2042
Fragments arranged by size:
2042 1483 1139 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2140 2832 2838 3246 3923 5071 5316 1606
Size: 534 692 6 408 677 1148 245 2120
Fragments arranged by size:
2120 1148 692 677 534 408 245 6
NaeI GCC'GGC
Cuts at: 2562 2788 2562
Size: 226 5604
NarI GG'CG_CC
Cuts at: 2614 2852 2614
Size: 238 5592
NcoI C'CATG_G
Cuts at: 513 1065 3120 513
Size: 552 2055 3223
Fragments arranged by size:
3223 2055 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NgoMIV G'CCGG_C
Cuts at: 2560 2786 2560
Size: 226 5604
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NsiI A_TGCA'T
Cuts at: 2862 2862
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 3188 4733 1132
Size: 97 1959 1545 2229
Fragments arranged by size:
2229 1959 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2605 2955 1108
Size: 1497 350 3983
Fragments arranged by size:
3983 1497 350
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PspOMI G'GGCC_C
Cuts at: 2316 2316
Size: 5830
PstI C_TGCA'G
Cuts at: 830 2837 3182 830
Size: 2007 345 3478
Fragments arranged by size:
3478 2007 345
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
PvuII CAG'CTG
Cuts at: 2140 2838 2140
Size: 698 5132
RsrII CG'GwC_CG
Cuts at: 2191 2191
Size: 5830
SacI G_AGCT'C
Cuts at: 2510 2577 2778 2510
Size: 67 201 5562
Fragments arranged by size:
5562 201 67
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2582 2582
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2430 2833 3178 4313 4994 5185 826
Size: 219 1385 403 345 1135 681 191 1471
Fragments arranged by size:
1471 1385 1135 681 403 345 219 191
SfoI GGC'GCC
Cuts at: 2615 2853 2615
Size: 238 5592
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 2366 3044 3157 1872
Size: 494 678 113 4545
Fragments arranged by size:
4545 678 494 113
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2030 3934 4835 5097 820
Size: 197 926 87 1904 901 262 1553
Fragments arranged by size:
1904 1553 926 901 262 197 87
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5830
StyI C'CwwG_G
Cuts at: 513 1065 3120 3269 513
Size: 552 2055 149 3074
Fragments arranged by size:
3074 2055 552 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2158 3857 3874 4027 4212 4657 325
Size: 1255 578 1699 17 153 185 445 1498
Fragments arranged by size:
1699 1498 1255 578 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 4076 5788
Size: 274 80 33 51 1443 165 1934 1712
Cuts at: 5788 96
Size: 138
Fragments arranged by size:
1934 1712 1443 274 165 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5790 5822 5790
Size: 32 5798
Tth111I GACn'n_nGTC
Cuts at: 1316 2195 1316
Size: 879 4951
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 2364 3042 3155 1870
Size: 494 678 113 4545
Fragments arranged by size:
4545 678 494 113
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI PacI PfoI PmlI PpuMI PspXI
PsrI SacII SanDI SexAI SfiI SrfI SwaI XcmI