Restriction map for
pFN21AE3156
(Circular) (Six-base) MAPSORT of: pFN21AE3156.seq Check: 5538 from: 1 to: 5830
pFN21AE3156 5830 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2247 2400 2469 2604 2756 3893 5277
Size: 422 193 153 69 135 152 1137 1384
Cuts at: 5277 5545 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1137 422 268 193 153 152
135 69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 4729 5706 1203
Size: 3526 977 1327
Fragments arranged by size:
3526 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 4559 4559
Size: 5830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3000 1184
Size: 1816 4014
AlwNI CAG_nnn'CTG
Cuts at: 2119 2603 3573 5145 2119
Size: 484 970 1572 2804
Fragments arranged by size:
2804 1572 970 484
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 2539 3139 3365 3517 5626 1493
Size: 1046 600 226 152 2109 1697
Fragments arranged by size:
2109 1697 1046 600 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 3155 1870
Size: 73 1212 4545
Fragments arranged by size:
4545 1212 73
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 3151 4606 618
Size: 325 217 627 335 1029 1455 1842
Fragments arranged by size:
1842 1455 1029 627 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2022 2643 3058 3149 1391
Size: 631 621 415 91 4072
Fragments arranged by size:
4072 631 621 415 91
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2235 2466 2573 928
Size: 971 336 231 107 4185
Fragments arranged by size:
4185 971 336 231 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4021 4055 1903
Size: 34 2084 34 3678
Fragments arranged by size:
3678 2084 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4441 136
Size: 107 122 71 631 605 2769 1525
Fragments arranged by size:
2769 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2775 5825 2775
Size: 3050 2780
BlpI GC'TnA_GC
Cuts at: 3247 3247
Size: 5830
Bme1580I G_kGCm'C
Cuts at: 1790 3891 5047 1790
Size: 2101 1156 2573
Fragments arranged by size:
2573 2101 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3561 4519 442
Size: 416 53 83 399 24 2144 958 1753
Fragments arranged by size:
2144 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2962 4490 1239
Size: 1723 1528 2579
Fragments arranged by size:
2579 1723 1528
Bpu10I CC'TnA_GC
Cuts at: 2343 2642 2343
Size: 299 5531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 3955 4820 5118 2006
Size: 1949 865 298 2718
Fragments arranged by size:
2718 1949 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4493 882
Size: 617 2994 2219
Fragments arranged by size:
2994 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3621 493
Size: 711 2417 2702
Fragments arranged by size:
2702 2417 711
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 3637 4019 4654
Size: 53 83 186 936 512 1592 382 635
Cuts at: 4654 275
Size: 1451
Fragments arranged by size:
1592 1451 936 635 512 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4263 4661 4935 5082 5493 4263
Size: 398 274 147 411 4600
Fragments arranged by size:
4600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2151 2181 2877 2907 1178
Size: 30 165 30 748 30 696 30 4101
Fragments arranged by size:
4101 748 696 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2919 1186
Size: 1733 4097
BseYI C'CCAG_C
Cuts at: 5033 5529 5033
Size: 496 5334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2090 2616 3035 1163
Size: 573 354 526 419 3958
Fragments arranged by size:
3958 573 526 419 354
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3280 4041 5069 1540
Size: 275 186 1279 761 1028 2301
Fragments arranged by size:
2301 1279 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2643 3891 3976 5047 2643
Size: 1248 85 1071 3426
Fragments arranged by size:
3426 1248 1071 85
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2643 3058 3149 3891 3976 5047
Size: 399 232 621 415 91 742 85 1071
Cuts at: 5047 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 621 415 399 232 91
85
BspEI T'CCGG_A
Cuts at: 5493 5493
Size: 5830
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3185 844
Size: 2341 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4474 4661 1700
Size: 110 2664 187 2869
Fragments arranged by size:
2869 2664 187 110
BsrGI T'GTAC_A
Cuts at: 96 3079 96
Size: 2983 2847
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5830
BssSI C'ACGA_G
Cuts at: 2066 3890 4902 2066
Size: 1824 1012 2994
Fragments arranged by size:
2994 1824 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2149 794
Size: 1355 4475
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstXI CCAn_nnnn'nTGG
Cuts at: 2591 2591
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 2775 3160 3527 3912 3929 5381 5467 5479
Size: 803 385 367 385 17 1452 86 12
Cuts at: 5479 5825 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 803 385 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2382 513
Size: 552 1317 3961
Fragments arranged by size:
3961 1317 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 2107 2170 2604 2953 3346 4140 4160 2107
Size: 63 434 349 393 794 20 3777
Fragments arranged by size:
3777 794 434 393 349 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2285 2320 529
Size: 35 1721 35 4039
Fragments arranged by size:
4039 1721 35 35
DraI TTT'AAA
Cuts at: 2589 3134 2589
Size: 545 5285
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3277 4166 5795
Size: 54 1425 50 275 18 1447 889 1629
Cuts at: 5795 8
Size: 43
Fragments arranged by size:
1629 1447 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3277 1537
Size: 275 1465 4090
Fragments arranged by size:
4090 1465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2418 2433 2484 3760 1353
Size: 1065 15 51 1276 3423
Fragments arranged by size:
3423 1276 1065 51 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2641 2641
Size: 5830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2247 2400 2469 2604 2756 2962
Size: 393 422 193 153 69 135 152 206
Cuts at: 2962 3893 4490 5277 5545 1239
Size: 931 597 787 268 1524
Fragments arranged by size:
1524 931 787 597 422 393 268 206
193 153 152 135 69
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 5830
FspI TGC'GCA
Cuts at: 4336 4336
Size: 5830
HaeII r_GCGC'y
Cuts at: 1678 4977 1678
Size: 3299 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2064 2096 2353 2385 4471
Size: 32 135 32 492 32 257 32 2086
Cuts at: 4471 4503 4545 4577 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2086 492 257 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2743 3174 3420 669
Size: 988 281 805 431 246 3079
Fragments arranged by size:
3079 988 805 431 281 246
HindIII A'AGCT_T
Cuts at: 748 2616 748
Size: 1868 3962
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 2297 3429 2297
Size: 1132 4698
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2069 4944 5128 1305
Size: 764 2875 184 2007
Fragments arranged by size:
2875 2007 764 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4008
Fragments arranged by size:
4008 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2209 2352 2589 3788 4147 4306
Size: 648 843 200 143 237 1199 359 159
Cuts at: 4306 518
Size: 2042
Fragments arranged by size:
2042 1199 843 648 359 237 200 159
143
MspA1I CmG'CkG
Cuts at: 1606 3246 3923 5071 5316 1606
Size: 1640 677 1148 245 2120
Fragments arranged by size:
2120 1640 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5278
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 2726 3188 4733 1132
Size: 97 1497 462 1545 2229
Fragments arranged by size:
2229 1545 1497 462 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5830
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2119 1108
Size: 1011 4819
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2021 2053 3899 3931 5412 5444 2021
Size: 32 1846 32 1481 32 2407
Fragments arranged by size:
2407 1846 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 2670 3400 2670
Size: 730 5100
PstI C_TGCA'G
Cuts at: 830 2327 2736 3182 830
Size: 1497 409 446 3478
Fragments arranged by size:
3478 1497 446 409
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
SacI G_AGCT'C
Cuts at: 2643 2643
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2484 2484
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2323 2732 3178 4313 4994 5185 826
Size: 219 1278 409 446 1135 681 191 1471
Fragments arranged by size:
1471 1278 1135 681 446 409 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 3934 4835 5097 820
Size: 197 926 78 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197 78
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 2550 3754 5
Size: 47 2498 1204 2081
Fragments arranged by size:
2498 2081 1204 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5830
StyI C'CwwG_G
Cuts at: 513 1065 3269 513
Size: 552 2204 3074
Fragments arranged by size:
3074 2204 552
SwaI ATTT'AAAT
Cuts at: 2589 2589
Size: 5830
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2888 2956 3857 3874 4027 4212 4657
Size: 1255 1308 68 901 17 153 185 445
Cuts at: 4657 325
Size: 1498
Fragments arranged by size:
1498 1308 1255 901 445 185 153 68
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3079 4076 5788
Size: 274 80 33 51 1443 1102 997 1712
Cuts at: 5788 96
Size: 138
Fragments arranged by size:
1712 1443 1102 997 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2151 2183 5790 5822 2151
Size: 32 3607 32 2159
Fragments arranged by size:
3607 2159 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 2260 3166 2260
Size: 906 4924
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI XcmI