Restriction map for
pFN21AE3154
(Circular) (Six-base) MAPSORT of: pFN21AE3154.seq Check: 9196 from: 1 to: 5821
pFN21AE3154 5821 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2249 3631 278
Size: 53 83 186 936 713 1382 2468
Fragments arranged by size:
2468 1382 936 713 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3884 5268 5536 1632
Size: 2252 1384 268 1917
Fragments arranged by size:
2252 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AlwNI CAG_nnn'CTG
Cuts at: 3564 5136 3564
Size: 1572 4249
ApaI G_GGCC'C
Cuts at: 2440 2674 2440
Size: 234 5587
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2340 2428 2537 2667 2792 2989 3146
Size: 73 397 88 109 130 125 197 157
Cuts at: 3146 1870
Size: 4545
Fragments arranged by size:
4545 397 197 157 130 125 109 88
73
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2193 2563 2603 2847
Size: 325 217 627 369 37 370 40 244
Cuts at: 2847 3142 4597 618
Size: 295 1455 1842
Fragments arranged by size:
1842 1455 627 370 369 325 295 244
217 40 37
BanII G_rGCy'C
Cuts at: 1391 2151 2440 2617 2674 3140 1391
Size: 760 289 177 57 466 4072
Fragments arranged by size:
4072 760 466 289 177 57
BbeI G_GCGC'C
Cuts at: 2197 2607 2197
Size: 410 5411
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2386 928
Size: 971 487 4363
Fragments arranged by size:
4363 971 487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4012 4046 1903
Size: 34 50 34 1991 34 3678
Fragments arranged by size:
3678 1991 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2612 2796 4432
Size: 107 122 71 631 605 940 184 1636
Cuts at: 4432 136
Size: 1525
Fragments arranged by size:
1636 1525 940 631 605 184 122 107
71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 2421 2498 3238 2421
Size: 77 740 5004
Fragments arranged by size:
5004 740 77
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2161 2440 2568 2674 3021 3882 5038
Size: 310 61 279 128 106 347 861 1156
Cuts at: 5038 1790
Size: 2573
Fragments arranged by size:
2573 1156 861 347 310 279 128 106
61
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2465 4481 1239
Size: 869 357 2016 2579
Fragments arranged by size:
2579 2016 869 357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2216 4484 882
Size: 617 717 2268 2219
Fragments arranged by size:
2268 2219 717 617
BsaAI yAC'GTr
Cuts at: 493 1204 2278 2824 3612 493
Size: 711 1074 546 788 2702
Fragments arranged by size:
2702 1074 788 711 546
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2194 2206 2246 2604
Size: 53 83 186 936 661 12 40 358
Cuts at: 2604 2766 3628 4010 4645 275
Size: 162 862 382 635 1451
Fragments arranged by size:
1451 936 862 661 635 382 358 186
162 83 53 40 12
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2213 2243 1178
Size: 30 165 30 810 30 4756
Fragments arranged by size:
4756 810 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2036 2039 2297 1186
Size: 847 3 3 258 4710
Fragments arranged by size:
4710 847 258 3 3
BseYI C'CCAG_C
Cuts at: 2107 2287 2490 2599 2853 5024 5520 2107
Size: 180 203 109 254 2171 496 2408
Fragments arranged by size:
2408 2171 496 254 203 180 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2174 2364 2756 2985 3271 4032
Size: 275 186 173 190 392 229 286 761
Cuts at: 4032 5060 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 392 286 275 229 190
186 173
BsiHKAI G_wGCw'C
Cuts at: 3048 3882 3967 5038 3048
Size: 834 85 1071 3831
Fragments arranged by size:
3831 1071 834 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2151 2161 2440 2568 2617 2674
Size: 399 310 51 10 279 128 49 57
Cuts at: 2674 3021 3048 3140 3882 3967 5038 1391
Size: 347 27 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 347 310 279 128
92 85 57 51 49 27 10
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3012 3176 844
Size: 2168 164 3489
Fragments arranged by size:
3489 2168 164
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2371 4316 4490 65
Size: 1183 1123 1945 174 1396
Fragments arranged by size:
1945 1396 1183 1123 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2173 2542 2941 4465 4652 1700
Size: 110 363 369 399 1524 187 2869
Fragments arranged by size:
2869 1524 399 369 363 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 2587 2685 2687 2808 2904 2965 2967 3023
Size: 662 98 2 121 96 61 2 56
Cuts at: 3023 1925
Size: 4723
Fragments arranged by size:
4723 662 121 98 96 61 56 2
2
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5821
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 2257 2716 1184
Size: 1073 459 4289
Fragments arranged by size:
4289 1073 459
BstYI r'GATC_y
Cuts at: 1972 3151 3518 3903 3920 5372 5458 5470 5816
Size: 1179 367 385 17 1452 86 12 346
Cuts at: 5816 1972
Size: 1977
Fragments arranged by size:
1977 1452 1179 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2326 2364 2756 513
Size: 552 1261 38 392 3578
Fragments arranged by size:
3578 1261 552 392 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2157 2845 487
Size: 683 56 931 688 3463
Fragments arranged by size:
3463 931 688 683 56
BtsI GCAGTG_nn'
Cuts at: 3337 4131 4151 3337
Size: 794 20 5007
Fragments arranged by size:
5007 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DraIII CAC_nnn'GTG
Cuts at: 2959 2959
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2171 2361
Size: 54 1425 50 275 18 202 139 190
Cuts at: 2361 2630 2694 2753 2880 2982 3268 4157 5786
Size: 269 64 59 127 102 286 889 1629
Cuts at: 5786 8
Size: 43
Fragments arranged by size:
1629 1425 889 286 275 269 202 190
139 127 102 64 59 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2171 2361 2753 2982 3268 1537
Size: 275 359 190 392 229 286 4090
Fragments arranged by size:
4090 392 359 286 275 229 190
EarI CTCTTCn'nnn_
Cuts at: 1353 3053 3751 1353
Size: 1700 698 3423
Fragments arranged by size:
3423 1700 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2257 2543 2633 4408 4782 4928
Size: 1064 138 668 286 90 1775 374 146
Cuts at: 4928 387
Size: 1280
Fragments arranged by size:
1775 1280 1064 668 374 286 146 138
90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2465 3884 4481 5268 5536 1239
Size: 393 476 357 1419 597 787 268 1524
Fragments arranged by size:
1524 1419 787 597 476 393 357 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2123 2696 2123
Size: 573 5248
EcoO109I rG'GnC_Cy
Cuts at: 2024 2212 2671 2024
Size: 188 459 5174
Fragments arranged by size:
5174 459 188
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2197 2428 2607 2654 2660 2802 2948 4968
Size: 519 231 179 47 6 142 146 2020
Cuts at: 4968 1678
Size: 2531
Fragments arranged by size:
2531 2020 519 231 179 146 142 47
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 4462 4494 4536
Size: 32 135 32 641 32 2217 32 42
Cuts at: 4536 4568 1373
Size: 32 2626
Fragments arranged by size:
2626 2217 641 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3165 3411 669
Size: 988 281 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KasI G'GCGC_C
Cuts at: 2193 2603 2193
Size: 410 5411
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4935 5119 1305
Size: 3630 184 2007
Fragments arranged by size:
3630 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2882 10
Size: 54 1425 343 1050 2949
Fragments arranged by size:
2949 1425 1050 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3041 3098 3779 4138 4297 518
Size: 648 1875 57 681 359 159 2042
Fragments arranged by size:
2042 1875 681 648 359 159 57
MspA1I CmG'CkG
Cuts at: 1606 2328 2366 2420 2758 2853 3237 3914 5062
Size: 722 38 54 338 95 384 677 1148
Cuts at: 5062 5307 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 722 677 384 338 245 95
54 38
NaeI GCC'GGC
Cuts at: 2943 2943
Size: 5821
NarI GG'CG_CC
Cuts at: 2194 2604 2194
Size: 410 5411
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2941 2941
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 2982 3268 2982
Size: 286 5535
NspI r_CATG'y
Cuts at: 1132 1229 3179 4724 1132
Size: 97 1950 1545 2229
Fragments arranged by size:
2229 1950 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2152 2252 2572 1348
Size: 804 100 320 4597
Fragments arranged by size:
4597 804 320 100
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2746 1108
Size: 1638 4183
PfoI T'CCnGG_A
Cuts at: 2319 2319
Size: 5821
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PmlI CAC'GTG
Cuts at: 2278 2278
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2024 2212 2024
Size: 188 5633
PshAI GACnn'nnGTC
Cuts at: 1613 2390 1613
Size: 777 5044
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PspOMI G'GGCC_C
Cuts at: 2436 2670 2436
Size: 234 5587
PstI C_TGCA'G
Cuts at: 830 3173 830
Size: 2343 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
RsrII CG'GwC_CG
Cuts at: 2323 2323
Size: 5821
SacII CC_GC'GG
Cuts at: 2329 2367 2759 2329
Size: 38 392 5391
Fragments arranged by size:
5391 392 38
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SanDI GG'GwC_CC
Cuts at: 2212 2212
Size: 5821
SapI GCTCTTCn'nnn_
Cuts at: 3053 3053
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SexAI A'CCwGG_T
Cuts at: 2225 2225
Size: 5821
SfcI C'TryA_G
Cuts at: 826 1045 3169 4304 4985 5176 826
Size: 219 2124 1135 681 191 1471
Fragments arranged by size:
2124 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2195 2605 2195
Size: 410 5411
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 2941 1700
Size: 1241 4580
SmaI CCC'GGG
Cuts at: 1872 2342 2539 2669 2794 2991 3148 1872
Size: 470 197 130 125 197 157 4545
Fragments arranged by size:
4545 470 197 197 157 130 125
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3925 4826 5088 820
Size: 197 926 1982 901 262 1553
Fragments arranged by size:
1982 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 2702 1517
Size: 1185 4636
StyI C'CwwG_G
Cuts at: 513 1065 3260 513
Size: 552 2195 3074
Fragments arranged by size:
3074 2195 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4067 5779 96
Size: 274 80 33 51 1443 2090 1712 138
Fragments arranged by size:
2090 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2292 2324 5781 5813 2292
Size: 32 3457 32 2300
Fragments arranged by size:
3457 2300 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2227 1316
Size: 911 4910
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 2340 2537 2667 2792 2989 3146 1870
Size: 470 197 130 125 197 157 4545
Fragments arranged by size:
4545 470 197 197 157 130 125
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2247 3629 276
Size: 53 83 186 936 713 1382 2468
Fragments arranged by size:
2468 1382 936 713 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PspXI PsrI
PvuII SacI SfiI SrfI SwaI XcmI