Restriction map for
pFN21AE3153
(Circular) (Six-base) MAPSORT of: pFN21AE3153.seq Check: 9270 from: 1 to: 5812
pFN21AE3153 5812 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3155 1937
Size: 1218 4594
Acc65I G'GTAC_C
Cuts at: 3133 3133
Size: 5812
AclI AA'CG_TT
Cuts at: 1563 3939 4312 1563
Size: 2376 373 3063
Fragments arranged by size:
3063 2376 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2879 3875 5259 5527 1632
Size: 1247 996 1384 268 1917
Fragments arranged by size:
1917 1384 1247 996 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5615
AflIII A'CryG_T
Cuts at: 1203 4711 5688 1203
Size: 3508 977 1327
Fragments arranged by size:
3508 1327 977
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 4541 4541
Size: 5812
AleI CACnn'nnGTG
Cuts at: 1166 2771 1166
Size: 1605 4207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5812
AlwNI CAG_nnn'CTG
Cuts at: 3555 5127 3555
Size: 1572 4240
ApaLI G'TGCA_C
Cuts at: 3869 5025 3869
Size: 1156 4656
ApoI r'AATT_y
Cuts at: 1493 2086 2190 2457 3121 3347 3499 5608 1493
Size: 593 104 267 664 226 152 2109 1697
Fragments arranged by size:
2109 1697 664 593 267 226 152 104
AseI AT'TA_AT
Cuts at: 160 1032 4366 160
Size: 872 3334 1606
Fragments arranged by size:
3334 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 3137 1870
Size: 73 1194 4545
Fragments arranged by size:
4545 1194 73
BamHI G'GATC_C
Cuts at: 3057 3142 3509 3057
Size: 85 367 5360
Fragments arranged by size:
5360 367 85
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3133 4588 618
Size: 325 217 627 1346 1455 1842
Fragments arranged by size:
1842 1455 1346 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2271 3131 1391
Size: 880 860 4072
Fragments arranged by size:
4072 880 860
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2904 928
Size: 971 1005 3836
Fragments arranged by size:
3836 1005 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4003 4037 1903
Size: 34 2066 34 3678
Fragments arranged by size:
3678 2066 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 2274 1548
Size: 726 5086
BfrBI ATG'CAT
Cuts at: 2288 2288
Size: 5812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4423 136
Size: 107 122 71 631 605 2751 1525
Fragments arranged by size:
2751 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2880 5807 2880
Size: 2927 2885
BlpI GC'TnA_GC
Cuts at: 2604 3229 2604
Size: 625 5187
Bme1580I G_kGCm'C
Cuts at: 1790 2593 3005 3873 5029 1790
Size: 803 412 868 1156 2573
Fragments arranged by size:
2573 1156 868 803 412
BmgBI CAC'GTC
Cuts at: 1318 2248 1318
Size: 930 4882
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3543 4501 442
Size: 416 53 83 399 24 2126 958 1753
Fragments arranged by size:
2126 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2556 2747 2981 4472 1239
Size: 1317 191 234 1491 2579
Fragments arranged by size:
2579 1491 1317 234 191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4802 5100 3937
Size: 865 298 4649
Fragments arranged by size:
4649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2846 4475 882
Size: 617 1347 1629 2219
Fragments arranged by size:
2219 1629 1347 617
BsaAI yAC'GTr
Cuts at: 493 1204 3603 493
Size: 711 2399 2702
Fragments arranged by size:
2702 2399 711
BsaBI GATnn'nnATC
Cuts at: 2798 5806 2798
Size: 3008 2804
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2919 3619 4001
Size: 53 83 186 936 485 901 700 382
Cuts at: 4001 4636 275
Size: 635 1451
Fragments arranged by size:
1451 936 901 700 635 485 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4245 4643 4917 5064 5475 4245
Size: 398 274 147 411 4582
Fragments arranged by size:
4582 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3062 3092 1178
Size: 30 165 30 1659 30 3898
Fragments arranged by size:
3898 1659 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5812
BseYI C'CCAG_C
Cuts at: 2720 5015 5511 2720
Size: 2295 496 3021
Fragments arranged by size:
3021 2295 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2171 1163
Size: 573 435 4804
Fragments arranged by size:
4804 573 435
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3262 4023 5051 1540
Size: 275 186 1261 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2271 3873 3958 5029 2271
Size: 1602 85 1071 3054
Fragments arranged by size:
3054 1602 1071 85
BsmI GAATG_Cn'
Cuts at: 2554 2680 3323 3416 2554
Size: 126 643 93 4950
Fragments arranged by size:
4950 643 126 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2271 2593 3005 3131 3873 3958 5029
Size: 399 481 322 412 126 742 85 1071
Cuts at: 5029 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 481 412 399 322 126
85
BspEI T'CCGG_A
Cuts at: 5475 5475
Size: 5812
BspHI T'CATG_A
Cuts at: 3701 5431 3701
Size: 1730 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2143 3167 844
Size: 1299 1024 3489
Fragments arranged by size:
3489 1299 1024
BsrBI CCG'CTC
Cuts at: 1394 1823 2125 3699 1394
Size: 429 302 1574 3507
Fragments arranged by size:
3507 1574 429 302
BsrDI GCAATG_nn'
Cuts at: 65 1248 4307 4481 65
Size: 1183 3059 174 1396
Fragments arranged by size:
3059 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4456 4643 1700
Size: 110 2646 187 2869
Fragments arranged by size:
2869 2646 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5812
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5812
BssSI C'ACGA_G
Cuts at: 3872 4884 3872
Size: 1012 4800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2352 794
Size: 1558 4254
BstBI TT'CG_AA
Cuts at: 2240 4616 2240
Size: 2376 3436
BstEII G'GTnAC_C
Cuts at: 1184 2962 1184
Size: 1778 4034
BstXI CCAn_nnnn'nTGG
Cuts at: 2263 2806 2263
Size: 543 5269
BstYI r'GATC_y
Cuts at: 1972 2532 2730 2880 3057 3142 3509 3894 3911
Size: 560 198 150 177 85 367 385 17
Cuts at: 3911 5363 5449 5461 5807 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 560 385 367 346 198 177
150 86 85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2691 513
Size: 552 1626 3634
Fragments arranged by size:
3634 1626 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5073
Fragments arranged by size:
5073 683 56
BtsI GCAGTG_nn'
Cuts at: 2357 2634 3328 4122 4142 2357
Size: 277 694 794 20 4027
Fragments arranged by size:
4027 794 694 277 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5777
DraI TTT'AAA
Cuts at: 3116 3116
Size: 5812
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4819 809
Size: 507 3503 1802
Fragments arranged by size:
3503 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2562 3259 4148
Size: 54 1425 50 275 18 732 697 889
Cuts at: 4148 5777 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 732 697 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3259 1537
Size: 275 1447 4090
Fragments arranged by size:
4090 1447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2523 2579 2931 3742 1353
Size: 1170 56 352 811 3423
Fragments arranged by size:
3423 1170 811 352 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2944 4399 4773 4919 387
Size: 1064 138 1355 1455 374 146 1280
Fragments arranged by size:
1455 1355 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2269 2269
Size: 5812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2556 2747 2879 2981 3875 4472 5259
Size: 393 924 191 132 102 894 597 787
Cuts at: 5259 5527 1239
Size: 268 1524
Fragments arranged by size:
1524 924 894 787 597 393 268 191
132 102
EcoNI CCTnn'n_nnAGG
Cuts at: 3041 3041
Size: 5812
EcoRI G'AATT_C
Cuts at: 2086 3121 3499 2086
Size: 1035 378 4399
Fragments arranged by size:
4399 1035 378
EcoRV GAT'ATC
Cuts at: 3025 3025
Size: 5812
FspI TGC'GCA
Cuts at: 4318 4318
Size: 5812
HaeII r_GCGC'y
Cuts at: 1678 2611 4959 1678
Size: 933 2348 2531
Fragments arranged by size:
2531 2348 933
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2642 2674 2881 2913 3062
Size: 32 135 32 1070 32 207 32 149
Cuts at: 3062 3094 4453 4485 4527 4559 1373
Size: 32 1359 32 42 32 2626
Fragments arranged by size:
2626 1359 1070 207 149 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2485 2830 3156 3402 669
Size: 988 281 547 345 326 246 3079
Fragments arranged by size:
3079 988 547 345 326 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5812
HpaI GTT'AAC
Cuts at: 3402 3402
Size: 5812
KpnI G_GTAC'C
Cuts at: 3137 3137
Size: 5812
MfeI C'AATT_G
Cuts at: 3411 3411
Size: 5812
MluI A'CGCG_T
Cuts at: 5688 5688
Size: 5812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2640 2764 3035 4926 5110 1305
Size: 1335 124 271 1891 184 2007
Fragments arranged by size:
2007 1891 1335 271 184 124
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2564 10
Size: 54 1425 343 732 3258
Fragments arranged by size:
3258 1425 732 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 2321 2771 2804 3770 4129 4288
Size: 648 1095 60 450 33 966 359 159
Cuts at: 4288 518
Size: 2042
Fragments arranged by size:
2042 1095 966 648 450 359 159 60
33
MspA1I CmG'CkG
Cuts at: 1606 3228 3905 5053 5298 1606
Size: 1622 677 1148 245 2120
Fragments arranged by size:
2120 1622 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2691 513
Size: 552 1626 3634
Fragments arranged by size:
3634 1626 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5812
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5812
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NsiI A_TGCA'T
Cuts at: 2290 2290
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 2292 3170 4715 1132
Size: 97 1063 878 1545 2229
Fragments arranged by size:
2229 1545 1063 878 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5812
PciI A'CATG_T
Cuts at: 4711 4711
Size: 5812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5812
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 5394 5426 3881
Size: 32 1481 32 4267
Fragments arranged by size:
4267 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 3382 3382
Size: 5812
PstI C_TGCA'G
Cuts at: 830 2848 3164 830
Size: 2018 316 3478
Fragments arranged by size:
3478 2018 316
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
SacI G_AGCT'C
Cuts at: 2271 2271
Size: 5812
SalI G'TCGA_C
Cuts at: 1936 3154 1936
Size: 1218 4594
SbfI CC_TGCA'GG
Cuts at: 3164 3164
Size: 5812
ScaI AGT'ACT
Cuts at: 4060 5772 4060
Size: 1712 4100
SfcI C'TryA_G
Cuts at: 826 1045 2844 3160 4295 4976 5167 826
Size: 219 1799 316 1135 681 191 1471
Fragments arranged by size:
1799 1471 1135 681 316 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 3139 1872
Size: 1267 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3916 4817 5079 820
Size: 197 926 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3603 493
Size: 3110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 3170 1132
Size: 2038 3774
SspI AAT'ATT
Cuts at: 5 52 2476 3736 5
Size: 47 2424 1260 2081
Fragments arranged by size:
2424 2081 1260 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5812
StyI C'CwwG_G
Cuts at: 513 1065 2691 3251 513
Size: 552 1626 560 3074
Fragments arranged by size:
3074 1626 560 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2704 2765 3839 3856 4009 4194 4639
Size: 1255 1124 61 1074 17 153 185 445
Cuts at: 4639 325
Size: 1498
Fragments arranged by size:
1498 1255 1124 1074 445 185 153 61
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2185 4058 5770
Size: 274 80 33 51 1443 208 1873 1712
Cuts at: 5770 96
Size: 138
Fragments arranged by size:
1873 1712 1443 274 208 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2613 2645 2970 3002 5772 5804 2613
Size: 32 325 32 2770 32 2621
Fragments arranged by size:
2770 2621 325 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5812
XbaI T'CTAG_A
Cuts at: 3148 3148
Size: 5812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2953 2059
Size: 894 4918
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5812
XmaI C'CCGG_G
Cuts at: 1870 3137 1870
Size: 1267 4545
XmnI GAAnn'nnTTC
Cuts at: 3941 3941
Size: 5812
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI