Restriction map for
pFN21AE3152
(Circular) (Six-base) MAPSORT of: pFN21AE3152.seq Check: 8322 from: 1 to: 5797
pFN21AE3152 5797 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 2315 3118 2315
Size: 803 4994
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2591 2709 2773 3051 3860 5244 5512 1632
Size: 959 118 64 278 809 1384 268 1917
Fragments arranged by size:
1917 1384 959 809 278 268 118 64
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 4696 5673 1203
Size: 3493 977 1327
Fragments arranged by size:
3493 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 2247 4526 2247
Size: 2279 3518
AleI CACnn'nnGTG
Cuts at: 1166 2114 1166
Size: 948 4849
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AlwNI CAG_nnn'CTG
Cuts at: 3540 5112 3540
Size: 1572 4225
ApaI G_GGCC'C
Cuts at: 2203 3040 2203
Size: 837 4960
ApaLI G'TGCA_C
Cuts at: 3020 3854 5010 3020
Size: 834 1156 3807
Fragments arranged by size:
3807 1156 834
ApoI r'AATT_y
Cuts at: 1493 2714 3106 3332 3484 5593 1493
Size: 1221 392 226 152 2109 1697
Fragments arranged by size:
2109 1697 1221 392 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2153 2234 2328 3122 1870
Size: 73 127 83 81 94 794 4545
Fragments arranged by size:
4545 794 127 94 83 81 73
BamHI G'GATC_C
Cuts at: 2413 3127 3494 2413
Size: 714 367 4716
Fragments arranged by size:
4716 714 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2126 2315 2907 2983 3118
Size: 325 217 627 339 189 592 76 135
Cuts at: 3118 4573 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 592 339 325 217 189
135 76
BanII G_rGCy'C
Cuts at: 1391 2203 2970 3040 3116 1391
Size: 812 767 70 76 4072
Fragments arranged by size:
4072 812 767 76 70
BbeI G_GCGC'C
Cuts at: 2130 2911 2987 2130
Size: 781 76 4940
Fragments arranged by size:
4940 781 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2274 928
Size: 971 375 4451
Fragments arranged by size:
4451 971 375
BbvCI CC'TCA_GC
Cuts at: 2516 2516
Size: 5797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2609 2643 3988 4022 1903
Size: 34 50 34 588 34 1345 34 3678
Fragments arranged by size:
3678 1345 588 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4408 136
Size: 107 122 71 631 605 2736 1525
Fragments arranged by size:
2736 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2203 3024 3040 3858 5014 1790
Size: 378 35 821 16 818 1156 2573
Fragments arranged by size:
2573 1156 821 818 378 35 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5797
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3528 4486 442
Size: 416 53 83 399 24 2111 958 1753
Fragments arranged by size:
2111 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2864 1056
Size: 1808 3989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2244 2844 2892 4457 1239
Size: 1005 600 48 1565 2579
Fragments arranged by size:
2579 1565 1005 600 48
Bpu10I CC'TnA_GC
Cuts at: 2516 2921 2516
Size: 405 5392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2657 3922 4787 5085 2657
Size: 1265 865 298 3369
Fragments arranged by size:
3369 1265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2234 2360 4460 882
Size: 617 735 126 2100 2219
Fragments arranged by size:
2219 2100 735 617 126
BsaAI yAC'GTr
Cuts at: 493 1204 3588 493
Size: 711 2384 2702
Fragments arranged by size:
2702 2384 711
BsaBI GATnn'nnATC
Cuts at: 2600 5791 2600
Size: 3191 2606
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2127 2231 2908 2984
Size: 53 83 186 936 594 104 677 76
Cuts at: 2984 3604 3986 4621 275
Size: 620 382 635 1451
Fragments arranged by size:
1451 936 677 635 620 594 382 186
104 83 76 53
BsaWI w'CCGG_w
Cuts at: 2931 4230 4628 4902 5049 5460 2931
Size: 1299 398 274 147 411 3268
Fragments arranged by size:
3268 1299 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5572
Fragments arranged by size:
5572 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2533 3068 1186
Size: 1347 535 3915
Fragments arranged by size:
3915 1347 535
BseYI C'CCAG_C
Cuts at: 2611 2699 2846 2876 3039 5000 5496 2611
Size: 88 147 30 163 1961 496 2912
Fragments arranged by size:
2912 1961 496 163 147 88 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2729 1163
Size: 573 993 4231
Fragments arranged by size:
4231 993 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2191 2563 2693 3247 4008 5036
Size: 275 186 190 372 130 554 761 1028
Cuts at: 5036 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 554 372 275 190 186
130
BsiHKAI G_wGCw'C
Cuts at: 2970 3024 3858 3943 5014 2970
Size: 54 834 85 1071 3753
Fragments arranged by size:
3753 1071 834 85 54
BsiWI C'GTAC_G
Cuts at: 2245 2245
Size: 5797
BsmI GAATG_Cn'
Cuts at: 2824 3308 3401 2824
Size: 484 93 5220
Fragments arranged by size:
5220 484 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2203 2970 3024 3040 3116 3858
Size: 399 378 35 767 54 16 76 742
Cuts at: 3858 3943 5014 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 767 742 399 378 85 76
54 35 16
BspEI T'CCGG_A
Cuts at: 2931 5460 2931
Size: 2529 3268
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3152 844
Size: 2308 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3684 1394
Size: 429 1861 3507
Fragments arranged by size:
3507 1861 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4292 4466 65
Size: 1183 3044 174 1396
Fragments arranged by size:
3044 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2123 2190 2562 2976 4441 4628 1700
Size: 110 313 67 372 414 1465 187 2869
Fragments arranged by size:
2869 1465 414 372 313 187 110 67
BsrGI T'GTAC_A
Cuts at: 96 2529 96
Size: 2433 3364
BssHII G'CGCG_C
Cuts at: 1925 2441 2443 1925
Size: 516 2 5279
Fragments arranged by size:
5279 516 2
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 2413 3127 3494 3879 3896 5348 5434 5446
Size: 441 714 367 385 17 1452 86 12
Cuts at: 5446 5792 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 714 441 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2185 2553 2632 513
Size: 552 1120 368 79 3678
Fragments arranged by size:
3678 1120 552 368 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 2251 2712 3313 4107 4127 2251
Size: 461 601 794 20 3921
Fragments arranged by size:
3921 794 601 461 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2188 2556
Size: 54 1425 50 275 18 288 70 368
Cuts at: 2556 2686 3244 4133 5762 8
Size: 130 558 889 1629 43
Fragments arranged by size:
1629 1425 889 558 368 288 275 130
70 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2188 3244 1537
Size: 275 376 1056 4090
Fragments arranged by size:
4090 1056 376 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2293 2339 2424 3727 1353
Size: 940 46 85 1303 3423
Fragments arranged by size:
3423 1303 940 85 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2226 2404 3071 4384 4758 4904
Size: 1064 138 637 178 667 1313 374 146
Cuts at: 4904 387
Size: 1280
Fragments arranged by size:
1313 1280 1064 667 637 374 178 146
138
EcoICRI GAG'CTC
Cuts at: 2968 2968
Size: 5797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2591 2709 2773 2844 2892 3051
Size: 393 612 347 118 64 71 48 159
Cuts at: 3051 3860 4457 5244 5512 1239
Size: 809 597 787 268 1524
Fragments arranged by size:
1524 809 787 612 597 393 347 268
159 118 71 64 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2211 2489 2211
Size: 278 5519
EcoO109I rG'GnC_Cy
Cuts at: 2199 2399 2746 3036 2199
Size: 200 347 290 4960
Fragments arranged by size:
4960 347 290 200
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2130 2911 2987 4944 1678
Size: 452 781 76 1957 2531
Fragments arranged by size:
2531 1957 781 452 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2233 2265 2420 2452 3041
Size: 32 135 32 661 32 155 32 589
Cuts at: 3041 3073 4438 4470 4512 4544 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1365 661 589 155 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2107 3141 3387 669
Size: 988 281 169 1034 246 3079
Fragments arranged by size:
3079 1034 988 281 246 169
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KasI G'GCGC_C
Cuts at: 2126 2907 2983 2126
Size: 781 76 4940
Fragments arranged by size:
4940 781 76
KpnI G_GTAC'C
Cuts at: 2319 3122 2319
Size: 803 4994
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 4911 5095 1305
Size: 1086 2520 184 2007
Fragments arranged by size:
2520 2007 1086 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 10
Size: 54 1425 343 288 3687
Fragments arranged by size:
3687 1425 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 3755 4114 4273 518
Size: 648 948 1641 359 159 2042
Fragments arranged by size:
2042 1641 948 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2187 2470 2569 2611 3213 3890 5038 5283
Size: 581 283 99 42 602 677 1148 245
Cuts at: 5283 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 602 581 283 245 99
42
NaeI GCC'GGC
Cuts at: 2192 2978 2192
Size: 786 5011
NarI GG'CG_CC
Cuts at: 2127 2908 2984 2127
Size: 781 76 4940
Fragments arranged by size:
4940 781 76
NcoI C'CATG_G
Cuts at: 513 1065 2632 513
Size: 552 1567 3678
Fragments arranged by size:
3678 1567 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NgoMIV G'CCGG_C
Cuts at: 2190 2976 2190
Size: 786 5011
NheI G'CTAG_C
Cuts at: 1052 2860 1052
Size: 1808 3989
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 3155 4700 1132
Size: 97 1926 1545 2229
Fragments arranged by size:
2229 1926 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2741 3046 1108
Size: 1633 305 3859
Fragments arranged by size:
3859 1633 305
PfoI T'CCnGG_A
Cuts at: 2361 2361
Size: 5797
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2746 2746
Size: 5797
PshAI GACnn'nnGTC
Cuts at: 1613 2666 1613
Size: 1053 4744
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PspOMI G'GGCC_C
Cuts at: 2199 3036 2199
Size: 837 4960
PstI C_TGCA'G
Cuts at: 830 3149 830
Size: 2319 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2470 2569 2611 2470
Size: 99 42 5656
Fragments arranged by size:
5656 99 42
SacI G_AGCT'C
Cuts at: 2970 2970
Size: 5797
SacII CC_GC'GG
Cuts at: 2188 2188
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 2730 2894 4045 5757 2730
Size: 164 1151 1712 2770
Fragments arranged by size:
2770 1712 1151 164
SfcI C'TryA_G
Cuts at: 826 1045 2656 3145 4280 4961 5152 826
Size: 219 1611 489 1135 681 191 1471
Fragments arranged by size:
1611 1471 1135 681 489 219 191
SfoI GGC'GCC
Cuts at: 2128 2909 2985 2128
Size: 781 76 4940
Fragments arranged by size:
4940 781 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 2123 1700
Size: 423 5374
SmaI CCC'GGG
Cuts at: 1872 2072 2155 2236 2330 3124 1872
Size: 200 83 81 94 794 4545
Fragments arranged by size:
4545 794 200 94 83 81
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2672 3901 4802 5064 820
Size: 197 926 729 1229 901 262 1553
Fragments arranged by size:
1553 1229 926 901 729 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 2484 2678 1517
Size: 967 194 4636
Fragments arranged by size:
4636 967 194
StyI C'CwwG_G
Cuts at: 513 1065 2168 2632 3236 513
Size: 552 1103 464 604 3074
Fragments arranged by size:
3074 1103 604 552 464
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3824 3841 3994 4179 4624 325
Size: 1255 2244 17 153 185 445 1498
Fragments arranged by size:
2244 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2529 2728 2892
Size: 274 80 33 51 1443 552 199 164
Cuts at: 2892 4043 5755 96
Size: 1151 1712 138
Fragments arranged by size:
1712 1443 1151 552 274 199 164 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5797
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 2070 2153 2234 2328 3122 1870
Size: 200 83 81 94 794 4545
Fragments arranged by size:
4545 794 200 94 83 81
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SanDI SapI SexAI SfiI SrfI SwaI XcmI