Restriction map for
pFN21AE3151
(Circular) (Six-base) MAPSORT of: pFN21AE3151.seq Check: 6900 from: 1 to: 5806
pFN21AE3151 5806 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2414 2485 2804 2414
Size: 71 319 5416
Fragments arranged by size:
5416 319 71
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3616 278
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3149 1937
Size: 1212 4594
Acc65I G'GTAC_C
Cuts at: 3127 3127
Size: 5806
AclI AA'CG_TT
Cuts at: 1563 3933 4306 1563
Size: 2370 373 3063
Fragments arranged by size:
3063 2370 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3869 5253 5521 1632
Size: 2237 1384 268 1917
Fragments arranged by size:
2237 1917 1384 268
AfeI AGC'GCT
Cuts at: 2879 2891 2879
Size: 12 5794
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5609
AflIII A'CryG_T
Cuts at: 1203 4705 5682 1203
Size: 3502 977 1327
Fragments arranged by size:
3502 1327 977
AgeI A'CCGG_T
Cuts at: 4637 4637
Size: 5806
AhdI GACnn_n'nnGTC
Cuts at: 4535 4535
Size: 5806
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5806
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5806
AlwNI CAG_nnn'CTG
Cuts at: 2929 3549 5121 2929
Size: 620 1572 3614
Fragments arranged by size:
3614 1572 620
ApaI G_GGCC'C
Cuts at: 2279 3017 2279
Size: 738 5068
ApaLI G'TGCA_C
Cuts at: 3863 5019 3863
Size: 1156 4650
ApoI r'AATT_y
Cuts at: 1493 3115 3341 3493 5602 1493
Size: 1622 226 152 2109 1697
Fragments arranged by size:
2109 1697 1622 226 152
AscI GG'CGCG_CC
Cuts at: 3030 3030
Size: 5806
AseI AT'TA_AT
Cuts at: 160 1032 4360 160
Size: 872 3328 1606
Fragments arranged by size:
3328 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2452 2620 3131 1870
Size: 73 126 383 168 511 4545
Fragments arranged by size:
4545 511 383 168 126 73
BamHI G'GATC_C
Cuts at: 2332 3136 3503 2332
Size: 804 367 4635
Fragments arranged by size:
4635 804 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2086 2125 2351 2371 2500
Size: 325 217 627 299 39 226 20 129
Cuts at: 2500 2509 3127 4582 618
Size: 9 618 1455 1842
Fragments arranged by size:
1842 1455 627 618 325 299 226 217
129 39 20 9
BanII G_rGCy'C
Cuts at: 1391 2279 2453 2621 2868 3017 3125 1391
Size: 888 174 168 247 149 108 4072
Fragments arranged by size:
4072 888 247 174 168 149 108
BbeI G_GCGC'C
Cuts at: 2090 2129 2504 2090
Size: 39 375 5392
Fragments arranged by size:
5392 375 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4835
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3997 4031 1903
Size: 34 2060 34 3678
Fragments arranged by size:
3678 2060 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3698 4908 3698
Size: 1210 4596
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5806
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2062 3047
Size: 107 122 71 631 605 367 23 985
Cuts at: 3047 4417 136
Size: 1370 1525
Fragments arranged by size:
1525 1370 985 631 605 367 122 107
71 23
BglII A'GATC_T
Cuts at: 2832 5801 2832
Size: 2969 2837
BlpI GC'TnA_GC
Cuts at: 2439 2615 2915 3223 2439
Size: 176 300 308 5022
Fragments arranged by size:
5022 308 300 176
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2279 2382 2391 3017 3867 5023 1790
Size: 222 267 103 9 626 850 1156 2573
Fragments arranged by size:
2573 1156 850 626 267 222 103 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5806
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3537 4495 442
Size: 416 53 83 399 24 2120 958 1753
Fragments arranged by size:
2120 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5806
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4466 1239
Size: 3227 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2150 2591 2831 2963 3931 4796 5094 2150
Size: 441 240 132 968 865 298 2862
Fragments arranged by size:
2862 968 865 441 298 240 132
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4469 882
Size: 617 2970 2219
Fragments arranged by size:
2970 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3597 493
Size: 711 2393 2702
Fragments arranged by size:
2702 2393 711
BsaBI GATnn'nnATC
Cuts at: 5800 5800
Size: 5806
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2087 2126 2501 2546
Size: 53 83 186 936 554 39 375 45
Cuts at: 2546 2762 3613 3995 4630 275
Size: 216 851 382 635 1451
Fragments arranged by size:
1451 936 851 635 554 382 375 216
186 83 53 45 39
BsaWI w'CCGG_w
Cuts at: 4239 4637 4911 5058 5469 4239
Size: 398 274 147 411 4576
Fragments arranged by size:
4576 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2819 2849 1178
Size: 30 165 30 1416 30 4135
Fragments arranged by size:
4135 1416 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2087 3034 1186
Size: 840 61 947 3958
Fragments arranged by size:
3958 947 840 61
BseYI C'CCAG_C
Cuts at: 2110 2485 5009 5505 2110
Size: 375 2524 496 2411
Fragments arranged by size:
2524 2411 496 375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2561 1163
Size: 573 825 4408
Fragments arranged by size:
4408 825 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2174 2436 2769 3051 3072 3256
Size: 275 186 173 262 333 282 21 184
Cuts at: 3256 4017 5045 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 333 282 275 262 186
184 173 21
BsiHKAI G_wGCw'C
Cuts at: 2184 3867 3952 5023 2184
Size: 1683 85 1071 2967
Fragments arranged by size:
2967 1683 1071 85
BsmI GAATG_Cn'
Cuts at: 3317 3410 3317
Size: 93 5713
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2184 2279 2382 2391 2453 2621
Size: 399 222 172 95 103 9 62 168
Cuts at: 2621 2868 3017 3125 3867 3952 5023 1391
Size: 247 149 108 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 247 222 172 168
149 108 103 95 85 62 9
BspEI T'CCGG_A
Cuts at: 5469 5469
Size: 5806
BspHI T'CATG_A
Cuts at: 3695 5425 3695
Size: 1730 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2414 2485 2733 2804 2953 3161 844
Size: 1570 71 248 71 149 208 3489
Fragments arranged by size:
3489 1570 248 208 149 71 71
BsrBI CCG'CTC
Cuts at: 1394 1823 2438 2658 3024 3693 1394
Size: 429 615 220 366 669 3507
Fragments arranged by size:
3507 669 615 429 366 220
BsrDI GCAATG_nn'
Cuts at: 65 1248 4301 4475 65
Size: 1183 3053 174 1396
Fragments arranged by size:
3053 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2263 2506 2552 4450 4637 1700
Size: 110 453 243 46 1898 187 2869
Fragments arranged by size:
2869 1898 453 243 187 110 46
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5806
BssHII G'CGCG_C
Cuts at: 1925 3030 1925
Size: 1105 4701
BssSI C'ACGA_G
Cuts at: 2858 3866 4878 2858
Size: 1008 1012 3786
Fragments arranged by size:
3786 1012 1008
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2929 794
Size: 2135 3671
BstBI TT'CG_AA
Cuts at: 4610 4610
Size: 5806
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5806
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 2365
Size: 5806
BstYI r'GATC_y
Cuts at: 1972 2332 2832 3136 3503 3888 3905 5357 5443
Size: 360 500 304 367 385 17 1452 86
Cuts at: 5443 5455 5801 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 500 385 367 360 346 304
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 2271 2391 2525 2644 513
Size: 552 939 90 177 120 134 119 3675
Fragments arranged by size:
3675 939 552 177 134 120 119 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5067
Fragments arranged by size:
5067 683 56
BtsI GCAGTG_nn'
Cuts at: 3322 4116 4136 3322
Size: 794 20 4992
Fragments arranged by size:
4992 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5806
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5771
DraI TTT'AAA
Cuts at: 3110 3110
Size: 5806
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2288 4813 809
Size: 507 972 2525 1802
Fragments arranged by size:
2525 1802 972 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2171 2214
Size: 54 1425 50 275 18 210 131 43
Cuts at: 2214 2433 2766 3048 3069 3253 4142 5771 8
Size: 219 333 282 21 184 889 1629 43
Fragments arranged by size:
1629 1425 889 333 282 275 219 210
184 131 54 50 43 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2171 2433 2766 3048 3069 3253 1537
Size: 275 359 262 333 282 21 184 4090
Fragments arranged by size:
4090 359 333 282 275 262 184 21
EarI CTCTTCn'nnn_
Cuts at: 1353 3736 1353
Size: 2383 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2212 2460 4393 4767 4913 387
Size: 1064 138 623 248 1933 374 146 1280
Fragments arranged by size:
1933 1280 1064 623 374 248 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3869 4466 5253 5521 1239
Size: 393 2237 597 787 268 1524
Fragments arranged by size:
2237 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2276 3013 2276
Size: 737 5069
EcoRI G'AATT_C
Cuts at: 3115 3493 3115
Size: 378 5428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 5806
FspI TGC'GCA
Cuts at: 4312 4312
Size: 5806
HaeII r_GCGC'y
Cuts at: 1678 2090 2111 2129 2195 2209 2504 2881 2893
Size: 412 21 18 66 14 295 377 12
Cuts at: 2893 4953 1678
Size: 2060 2531
Fragments arranged by size:
2531 2060 412 377 295 66 21 18
14 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2402 2434 2747 2779 4447
Size: 32 135 32 830 32 313 32 1668
Cuts at: 4447 4479 4521 4553 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1668 830 313 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3150 3396 669
Size: 988 281 1212 246 3079
Fragments arranged by size:
3079 1212 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5806
HpaI GTT'AAC
Cuts at: 3396 3396
Size: 5806
KasI G'GCGC_C
Cuts at: 2086 2125 2500 2086
Size: 39 375 5392
Fragments arranged by size:
5392 375 39
KpnI G_GTAC'C
Cuts at: 3131 3131
Size: 5806
MfeI C'AATT_G
Cuts at: 3405 3405
Size: 5806
MluI A'CGCG_T
Cuts at: 5682 5682
Size: 5806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4920 5104 1305
Size: 3615 184 2007
Fragments arranged by size:
3615 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3984
Fragments arranged by size:
3984 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3764 4123 4282 518
Size: 648 2598 359 159 2042
Fragments arranged by size:
2598 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2096 2393 2527 2646 2911 3006 3063 3222
Size: 490 297 134 119 265 95 57 159
Cuts at: 3222 3899 5047 5292 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 490 297 265 245 159
134 119 95 57
NaeI GCC'GGC
Cuts at: 2265 2265
Size: 5806
NarI GG'CG_CC
Cuts at: 2087 2126 2501 2087
Size: 39 375 5392
Fragments arranged by size:
5392 375 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4315
Fragments arranged by size:
4315 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5806
NgoMIV G'CCGG_C
Cuts at: 2263 2263
Size: 5806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5806
NotI GC'GGCC_GC
Cuts at: 2433 3253 2433
Size: 820 4986
NspI r_CATG'y
Cuts at: 1132 1229 3164 4709 1132
Size: 97 1935 1545 2229
Fragments arranged by size:
2229 1935 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5806
PciI A'CATG_T
Cuts at: 4705 4705
Size: 5806
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3084 1108
Size: 1976 3830
PmeI GTTT'AAAC
Cuts at: 3110 3110
Size: 5806
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3875 3907 5388 5420 3875
Size: 32 1481 32 4261
Fragments arranged by size:
4261 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5806
PsiI TTA'TAA
Cuts at: 3376 3376
Size: 5806
PspOMI G'GGCC_C
Cuts at: 2275 3013 2275
Size: 738 5068
PstI C_TGCA'G
Cuts at: 830 2673 2781 3158 830
Size: 1843 108 377 3478
Fragments arranged by size:
3478 1843 377 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5806
PvuII CAG'CTG
Cuts at: 3063 3063
Size: 5806
SacII CC_GC'GG
Cuts at: 2097 2394 2528 2647 2097
Size: 297 134 119 5256
Fragments arranged by size:
5256 297 134 119
SalI G'TCGA_C
Cuts at: 1936 3148 1936
Size: 1212 4594
SbfI CC_TGCA'GG
Cuts at: 3158 3158
Size: 5806
ScaI AGT'ACT
Cuts at: 4054 5766 4054
Size: 1712 4094
SexAI A'CCwGG_T
Cuts at: 2709 2709
Size: 5806
SfcI C'TryA_G
Cuts at: 826 1045 2669 2777 3154 4289 4970 5161 826
Size: 219 1624 108 377 1135 681 191 1471
Fragments arranged by size:
1624 1471 1135 681 377 219 191 108
SfoI GGC'GCC
Cuts at: 2088 2127 2502 2088
Size: 39 375 5392
Fragments arranged by size:
5392 375 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5806
SgrAI Cr'CCGG_yG
Cuts at: 1700 2506 2552 1700
Size: 806 46 4954
Fragments arranged by size:
4954 806 46
SmaI CCC'GGG
Cuts at: 1872 2622 3133 1872
Size: 750 511 4545
Fragments arranged by size:
4545 750 511
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2165 2606 2846 2978 3910 4811
Size: 197 926 222 441 240 132 932 901
Cuts at: 4811 5073 820
Size: 262 1553
Fragments arranged by size:
1553 932 926 901 441 262 240 222
197 132
SnaBI TAC'GTA
Cuts at: 493 3597 493
Size: 3104 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5806
SphI G_CATG'C
Cuts at: 1132 3164 1132
Size: 2032 3774
SrfI GCCC'GGGC
Cuts at: 2622 2622
Size: 5806
SspI AAT'ATT
Cuts at: 5 52 3730 5
Size: 47 3678 2081
Fragments arranged by size:
3678 2081 47
StuI AGG'CCT
Cuts at: 1517 2690 1517
Size: 1173 4633
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 3245 513
Size: 552 939 39 1202 3074
Fragments arranged by size:
3074 1202 939 552 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2272 3833 3850 4003 4188 4633 325
Size: 1255 692 1561 17 153 185 445 1498
Fragments arranged by size:
1561 1498 1255 692 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4052 5764 96
Size: 274 80 33 51 1443 2075 1712 138
Fragments arranged by size:
2075 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5766 5798 5766
Size: 32 5774
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5806
XbaI T'CTAG_A
Cuts at: 3142 3142
Size: 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5806
XmaI C'CCGG_G
Cuts at: 1870 2620 3131 1870
Size: 750 511 4545
Fragments arranged by size:
4545 750 511
XmnI GAAnn'nnTTC
Cuts at: 3935 3935
Size: 5806
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3614 276
Size: 53 83 186 936 2080 2468
Fragments arranged by size:
2468 2080 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SfiI SwaI XcmI