Restriction map for
pFN21AE3150
(Circular) (Six-base) MAPSORT of: pFN21AE3150.seq Check: 6677 from: 1 to: 5779
pFN21AE3150 5779 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2854 3122 1937
Size: 917 268 4594
Fragments arranged by size:
4594 917 268
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2636 2897 3842 5226 5494 1632
Size: 1004 261 945 1384 268 1917
Fragments arranged by size:
1917 1384 1004 945 268 261
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 4678 5655 1203
Size: 3475 977 1327
Fragments arranged by size:
3475 1327 977
AgeI A'CCGG_T
Cuts at: 2727 4610 2727
Size: 1883 3896
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 3041 1166
Size: 1875 3904
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2205 2415 1184
Size: 1021 210 4548
Fragments arranged by size:
4548 1021 210
AlwNI CAG_nnn'CTG
Cuts at: 3522 5094 3522
Size: 1572 4207
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 3104 1870
Size: 73 1161 4545
Fragments arranged by size:
4545 1161 73
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3100 4555 618
Size: 325 217 627 1313 1455 1842
Fragments arranged by size:
1842 1455 1313 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3098 1391
Size: 1707 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2802 928
Size: 971 903 3905
Fragments arranged by size:
3905 971 903
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2945 2979 3970 4004 1903
Size: 34 50 34 924 34 991 34 3678
Fragments arranged by size:
3678 991 924 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BfrBI ATG'CAT
Cuts at: 2192 2192
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4390 136
Size: 107 122 71 631 605 2718 1525
Fragments arranged by size:
2718 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2030 2515 5774 2030
Size: 485 3259 2035
Fragments arranged by size:
3259 2035 485
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 3840 4996 1790
Size: 2050 1156 2573
Fragments arranged by size:
2573 2050 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2404 3510 4468
Size: 416 53 83 399 24 987 1106 958
Cuts at: 4468 442
Size: 1753
Fragments arranged by size:
1753 1106 987 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2047 4439 1239
Size: 808 2392 2579
Fragments arranged by size:
2579 2392 808
Bpu10I CC'TnA_GC
Cuts at: 2531 2531
Size: 5779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2326 3904 4769 5067 2326
Size: 1578 865 298 3038
Fragments arranged by size:
3038 1578 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4442 882
Size: 617 2943 2219
Fragments arranged by size:
2943 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3570 493
Size: 711 2366 2702
Fragments arranged by size:
2702 2366 711
BsaBI GATnn'nnATC
Cuts at: 2080 5773 2080
Size: 3693 2086
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3586 3968 4603 275
Size: 53 83 186 936 2053 382 635 1451
Fragments arranged by size:
2053 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2727 4212 4610 4884 5031 5442 2727
Size: 1485 398 274 147 411 3064
Fragments arranged by size:
3064 1485 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5554
Fragments arranged by size:
5554 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2574 1186
Size: 1388 4391
BseYI C'CCAG_C
Cuts at: 2926 4982 5478 2926
Size: 2056 496 3227
Fragments arranged by size:
3227 2056 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2687 2992 1163
Size: 573 951 305 3950
Fragments arranged by size:
3950 951 573 305
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3018 3229 3990 5018 1540
Size: 275 186 1017 211 761 1028 2301
Fragments arranged by size:
2301 1028 1017 761 275 211 186
BsiHKAI G_wGCw'C
Cuts at: 3840 3925 4996 3840
Size: 85 1071 4623
Fragments arranged by size:
4623 1071 85
BsmI GAATG_Cn'
Cuts at: 2547 2976 3290 3383 2547
Size: 429 314 93 4943
Fragments arranged by size:
4943 429 314 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3098 3840 3925 4996 1391
Size: 399 1308 742 85 1071 2174
Fragments arranged by size:
2174 1308 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3134 844
Size: 2290 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 1394
Size: 429 1843 3507
Fragments arranged by size:
3507 1843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2727 4423 4610 1700
Size: 110 917 1696 187 2869
Fragments arranged by size:
2869 1696 917 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 3839 4851 3839
Size: 1012 4767
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5779
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 2767 1184
Size: 1583 4196
BstYI r'GATC_y
Cuts at: 1972 2030 2262 2515 2647 3109 3476 3861 3878
Size: 58 232 253 132 462 367 385 17
Cuts at: 3878 5330 5416 5428 5774 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 462 385 367 346 253 232
132 86 58 17 12
Bsu36I CC'TnA_GG
Cuts at: 2758 2758
Size: 5779
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 2951 2968 3295 4089 4109 2951
Size: 17 327 794 20 4621
Fragments arranged by size:
4621 794 327 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3015 3226 4115
Size: 54 1425 50 275 18 1185 211 889
Cuts at: 4115 5744 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1185 889 275 211 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3015 3226 1537
Size: 275 1203 211 4090
Fragments arranged by size:
4090 1203 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2172 3074 3709 1353
Size: 713 106 902 635 3423
Fragments arranged by size:
3423 902 713 635 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4366 4740 4886 387
Size: 1064 138 2777 374 146 1280
Fragments arranged by size:
2777 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2636 2897 3842 4439 5226 5494
Size: 393 415 589 261 945 597 787 268
Cuts at: 5494 1239
Size: 1524
Fragments arranged by size:
1524 945 787 597 589 415 393 268
261
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
EcoRV GAT'ATC
Cuts at: 2359 2359
Size: 5779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 5779
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 4926 1678
Size: 3248 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2519 2551 2733
Size: 32 135 32 443 32 472 32 182
Cuts at: 2733 2765 2847 2879 4420 4452 4494 4526 1373
Size: 32 82 32 1541 32 42 32 2626
Fragments arranged by size:
2626 1541 472 443 182 135 82 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2415 3123 3369 669
Size: 988 281 477 708 246 3079
Fragments arranged by size:
3079 988 708 477 281 246
HindIII A'AGCT_T
Cuts at: 748 2816 748
Size: 2068 3711
HpaI GTT'AAC
Cuts at: 2415 3369 2415
Size: 954 4825
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2027 4893 5077 1305
Size: 722 2866 184 2007
Fragments arranged by size:
2866 2007 722 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3957
Fragments arranged by size:
3957 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 3041 3737 4096 4255 518
Size: 648 1021 854 696 359 159 2042
Fragments arranged by size:
2042 1021 854 696 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2806 3030 3195 3872 5020 5265 1606
Size: 1200 224 165 677 1148 245 2120
Fragments arranged by size:
2120 1200 1148 677 245 224 165
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5227
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NsiI A_TGCA'T
Cuts at: 2194 2194
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2710 3137 4682 1132
Size: 97 1481 427 1545 2229
Fragments arranged by size:
2229 1545 1481 427 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5779
PfoI T'CCnGG_A
Cuts at: 2035 2035
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PstI C_TGCA'G
Cuts at: 830 2975 3131 830
Size: 2145 156 3478
Fragments arranged by size:
3478 2145 156
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2806 3030 2806
Size: 224 5555
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SapI GCTCTTCn'nnn_
Cuts at: 2172 2172
Size: 5779
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SfcI C'TryA_G
Cuts at: 826 1045 2642 2971 3127 4262 4943 5134 826
Size: 219 1597 329 156 1135 681 191 1471
Fragments arranged by size:
1597 1471 1135 681 329 219 191 156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2341 3883 4784 5046 820
Size: 197 926 398 1542 901 262 1553
Fragments arranged by size:
1553 1542 926 901 398 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 2166 2596 3703 5
Size: 47 2114 430 1107 2081
Fragments arranged by size:
2114 2081 1107 430 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5779
StyI C'CwwG_G
Cuts at: 513 1065 2310 3218 513
Size: 552 1245 908 3074
Fragments arranged by size:
3074 1245 908 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3806 3823 3976 4161 4606 325
Size: 1255 2226 17 153 185 445 1498
Fragments arranged by size:
2226 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2901 4025 5737
Size: 274 80 33 51 1443 924 1124 1712
Cuts at: 5737 96
Size: 138
Fragments arranged by size:
1712 1443 1124 924 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2062 2094 5739 5771 2062
Size: 32 3645 32 2070
Fragments arranged by size:
3645 2070 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 2447 2619 3908 2447
Size: 172 1289 4318
Fragments arranged by size:
4318 1289 172
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI