Restriction map for
pFN21AE3149
(Circular) (Six-base) MAPSORT of: pFN21AE3149.seq Check: 6415 from: 1 to: 5800
pFN21AE3149 5800 bp
With 182 enzymes: SgfI *
March 28, 2012 13:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 2874 3121 2874
Size: 247 5553
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2665 3863 5247 5515 1632
Size: 1033 1198 1384 268 1917
Fragments arranged by size:
1917 1384 1198 1033 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 2227 1166
Size: 1061 4739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 2443 2481 3543 5115 2443
Size: 38 1062 1572 3128
Fragments arranged by size:
3128 1572 1062 38
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 2216 3109 3335 3487 5596 1493
Size: 723 893 226 152 2109 1697
Fragments arranged by size:
2109 1697 893 723 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 2934 3125 1870
Size: 73 991 191 4545
Fragments arranged by size:
4545 991 191 73
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2874 3121 4576 618
Size: 325 217 627 372 715 247 1455 1842
Fragments arranged by size:
1842 1455 715 627 372 325 247 217
BanII G_rGCy'C
Cuts at: 1391 3119 1391
Size: 1728 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3087 928
Size: 971 1188 3641
Fragments arranged by size:
3641 1188 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 2292 2835 1548
Size: 744 543 4513
Fragments arranged by size:
4513 744 543
BfrBI ATG'CAT
Cuts at: 2557 2557
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4411 136
Size: 107 122 71 631 605 2739 1525
Fragments arranged by size:
2739 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 2330 3217 2330
Size: 887 4913
Bme1580I G_kGCm'C
Cuts at: 1790 3861 5017 1790
Size: 2071 1156 2573
Fragments arranged by size:
2573 2071 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2049 2431 3531
Size: 416 53 83 399 24 632 382 1100
Cuts at: 3531 4489 442
Size: 958 1753
Fragments arranged by size:
1753 1100 958 632 416 399 382 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2334 2693 2334
Size: 359 5441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4460 1239
Size: 3221 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2914 4463 882
Size: 617 1415 1549 2219
Fragments arranged by size:
2219 1549 1415 617
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3607 3989 4624 275
Size: 53 83 186 936 2074 382 635 1451
Fragments arranged by size:
2074 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5575
Fragments arranged by size:
5575 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5800
BseYI C'CCAG_C
Cuts at: 5003 5499 5003
Size: 496 5304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5227
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3099 3250 4011 5039 1540
Size: 275 186 1098 151 761 1028 2301
Fragments arranged by size:
2301 1098 1028 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 2572 3861 3946 5017 2572
Size: 1289 85 1071 3355
Fragments arranged by size:
3355 1289 1071 85
BsmI GAATG_Cn'
Cuts at: 2559 2799 3311 3404 2559
Size: 240 512 93 4955
Fragments arranged by size:
4955 512 240 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2572 3119 3861 3946 5017 1391
Size: 399 782 547 742 85 1071 2174
Fragments arranged by size:
2174 1071 782 742 547 399 85
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 2289 3689 5419 2289
Size: 1400 1730 2670
Fragments arranged by size:
2670 1730 1400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3155 844
Size: 2311 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2125 2244 2838 4295 4469 65
Size: 1183 877 119 594 1457 174 1396
Fragments arranged by size:
1457 1396 1183 877 594 174 119
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 2302 2977 96
Size: 2206 675 2919
Fragments arranged by size:
2919 2206 675
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 2229 2229
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795
Size: 1158 367 385 17 1452 86 12 346
Cuts at: 5795 1972
Size: 1977
Fragments arranged by size:
1977 1452 1158 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 3316 4110 4130 3316
Size: 794 20 4986
Fragments arranged by size:
4986 794 20
ClaI AT'CG_AT
Cuts at: 1554 2829 1554
Size: 1275 4525
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DraIII CAC_nnn'GTG
Cuts at: 2439 2439
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2886 3008 3247
Size: 54 1425 50 275 18 1056 122 239
Cuts at: 3247 4136 5765 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1056 889 275 239 122 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2684 2917 3730 1353
Size: 1331 233 813 3423
Fragments arranged by size:
3423 1331 813 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2665 3863 4460 5247 5515 1239
Size: 393 1033 1198 597 787 268 1524
Fragments arranged by size:
1524 1198 1033 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2314 2314
Size: 5800
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
EcoRV GAT'ATC
Cuts at: 2011 2011
Size: 5800
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 4947 1678
Size: 3269 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2415 2447 2643 2675 4441
Size: 32 135 32 843 32 196 32 1766
Cuts at: 4441 4473 4515 4547 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1766 843 196 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 2878 3125 2878
Size: 247 5553
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2401 4914 5098 1305
Size: 1096 2513 184 2007
Fragments arranged by size:
2513 2007 1096 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2888 10
Size: 54 1425 343 1056 2922
Fragments arranged by size:
2922 1425 1056 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2227 2522 3758 4117 4276 518
Size: 648 1061 295 1236 359 159 2042
Fragments arranged by size:
2042 1236 1061 648 359 295 159
MspA1I CmG'CkG
Cuts at: 1606 2087 2685 3013 3216 3893 5041 5286 1606
Size: 481 598 328 203 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 598 481 328 245 203
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NsiI A_TGCA'T
Cuts at: 2559 2559
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2884 1108
Size: 1776 4024
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2314 2314
Size: 5800
PshAI GACnn'nnGTC
Cuts at: 1613 2285 1613
Size: 672 5128
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PstI C_TGCA'G
Cuts at: 830 3152 830
Size: 2322 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SanDI GG'GwC_CC
Cuts at: 2314 2314
Size: 5800
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 3148 4283 4964 5155 826
Size: 219 2103 1135 681 191 1471
Fragments arranged by size:
2103 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2934 3904 4805 5067 820
Size: 197 926 991 970 901 262 1553
Fragments arranged by size:
1553 991 970 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 2732 3724 5
Size: 47 2680 992 2081
Fragments arranged by size:
2680 2081 992 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5800
StyI C'CwwG_G
Cuts at: 513 1065 2004 2322 3239 513
Size: 552 939 318 917 3074
Fragments arranged by size:
3074 939 917 552 318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2450 3827 3844 3997 4182 4627 325
Size: 1255 870 1377 17 153 185 445 1498
Fragments arranged by size:
1498 1377 1255 870 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2302 2977 4046
Size: 274 80 33 51 1443 325 675 1069
Cuts at: 4046 5758 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1069 675 325 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5760 5792 5760
Size: 32 5768
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2229 2229
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 2934 1943
Size: 991 4809
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI