Restriction map for
pFN21AE3148
(Circular) (Six-base) MAPSORT of: pFN21AE3148.seq Check: 9788 from: 1 to: 5791
pFN21AE3148 5791 bp
With 182 enzymes: SgfI *
November 18, 2016 17:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3601 278
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2034 3134 1937
Size: 97 1100 4594
Fragments arranged by size:
4594 1100 97
Acc65I G'GTAC_C
Cuts at: 3112 3112
Size: 5791
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2972 3854 5238 5506 1632
Size: 1340 882 1384 268 1917
Fragments arranged by size:
1917 1384 1340 882 268
AflII C'TTAA_G
Cuts at: 820 1017 2437 820
Size: 197 1420 4174
Fragments arranged by size:
4174 1420 197
AflIII A'CryG_T
Cuts at: 1203 4690 5667 1203
Size: 3487 977 1327
Fragments arranged by size:
3487 1327 977
AgeI A'CCGG_T
Cuts at: 2641 4622 2641
Size: 1981 3810
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5791
AlwNI CAG_nnn'CTG
Cuts at: 2210 2593 3534 5106 2210
Size: 383 941 1572 2895
Fragments arranged by size:
2895 1572 941 383
ApaLI G'TGCA_C
Cuts at: 3848 5004 3848
Size: 1156 4635
ApoI r'AATT_y
Cuts at: 1493 2171 2677 2769 3028 3100 3326 3478 5587
Size: 678 506 92 259 72 226 152 2109
Cuts at: 5587 1493
Size: 1697
Fragments arranged by size:
2109 1697 678 506 259 226 152 92
72
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 3116 1870
Size: 73 1173 4545
Fragments arranged by size:
4545 1173 73
BamHI G'GATC_C
Cuts at: 3121 3488 3121
Size: 367 5424
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 2702 3112 4567 618
Size: 325 217 627 360 555 410 1455 1842
Fragments arranged by size:
1842 1455 627 555 410 360 325 217
BanII G_rGCy'C
Cuts at: 1391 2044 2364 3110 1391
Size: 653 320 746 4072
Fragments arranged by size:
4072 746 653 320
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3982 4016 1903
Size: 34 2045 34 3678
Fragments arranged by size:
3678 2045 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2441 3683 4893 2441
Size: 1242 1210 3339
Fragments arranged by size:
3339 1242 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4402 136
Size: 107 122 71 631 605 2730 1525
Fragments arranged by size:
2730 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5786 5786
Size: 5791
BlpI GC'TnA_GC
Cuts at: 2043 3208 2043
Size: 1165 4626
Bme1580I G_kGCm'C
Cuts at: 1790 3852 5008 1790
Size: 2062 1156 2573
Fragments arranged by size:
2573 2062 1156
BmgBI CAC'GTC
Cuts at: 1318 2799 1318
Size: 1481 4310
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2047 2876 3522
Size: 416 53 83 399 24 630 829 646
Cuts at: 3522 4480 442
Size: 958 1753
Fragments arranged by size:
1753 958 829 646 630 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 4451 1239
Size: 962 2250 2579
Fragments arranged by size:
2579 2250 962
Bpu10I CC'TnA_GC
Cuts at: 2370 2370
Size: 5791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2121 3916 4781 5079 2121
Size: 1795 865 298 2833
Fragments arranged by size:
2833 1795 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2915 4454 882
Size: 617 1416 1539 2219
Fragments arranged by size:
2219 1539 1416 617
BsaAI yAC'GTr
Cuts at: 493 1204 3582 493
Size: 711 2378 2702
Fragments arranged by size:
2702 2378 711
BsaBI GATnn'nnATC
Cuts at: 5785 5785
Size: 5791
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2024 3598 3980 4615
Size: 53 83 186 936 491 1574 382 635
Cuts at: 4615 275
Size: 1451
Fragments arranged by size:
1574 1451 936 635 491 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2641 2924 4224 4622 4896 5043 5454 2641
Size: 283 1300 398 274 147 411 2978
Fragments arranged by size:
2978 1300 411 398 283 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2148 2178 1178
Size: 30 165 30 745 30 4791
Fragments arranged by size:
4791 745 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5791
BseYI C'CCAG_C
Cuts at: 4994 5490 4994
Size: 496 5295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5218
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4002 5030 1540
Size: 275 186 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 2826 3852 3937 5008 2364
Size: 462 1026 85 1071 3147
Fragments arranged by size:
3147 1071 1026 462 85
BsmI GAATG_Cn'
Cuts at: 3302 3395 3302
Size: 93 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2364 2826 3110 3852 3937 5008
Size: 399 254 320 462 284 742 85 1071
Cuts at: 5008 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 462 399 320 284 254
85
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 3680 5410 3680
Size: 1730 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2224 3146 844
Size: 1380 922 3489
Fragments arranged by size:
3489 1380 922
BsrBI CCG'CTC
Cuts at: 1394 1823 3678 1394
Size: 429 1855 3507
Fragments arranged by size:
3507 1855 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2942 4286 4460 65
Size: 1183 1694 1344 174 1396
Fragments arranged by size:
1694 1396 1344 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2641 4435 4622 1700
Size: 110 831 1794 187 2869
Fragments arranged by size:
2869 1794 831 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5791
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5791
BssSI C'ACGA_G
Cuts at: 3851 4863 3851
Size: 1012 4779
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5791
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2669 2669
Size: 5791
BstYI r'GATC_y
Cuts at: 1972 2493 3084 3121 3488 3873 3890 5342 5428
Size: 521 591 37 367 385 17 1452 86
Cuts at: 5428 5440 5786 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 591 521 385 367 346 86
37 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2706 513
Size: 552 939 702 3598
Fragments arranged by size:
3598 939 702 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5052
Fragments arranged by size:
5052 683 56
BtsI GCAGTG_nn'
Cuts at: 3307 4101 4121 3307
Size: 794 20 4977
Fragments arranged by size:
4977 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2292 2327 529
Size: 35 1728 35 3993
Fragments arranged by size:
3993 1728 35 35
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DraIII CAC_nnn'GTG
Cuts at: 2847 2847
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4798 809
Size: 507 3482 1802
Fragments arranged by size:
3482 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3238 4127 5756
Size: 54 1425 50 275 18 1408 889 1629
Cuts at: 5756 8
Size: 43
Fragments arranged by size:
1629 1425 1408 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2373 3721 1353
Size: 1020 1348 3423
Fragments arranged by size:
3423 1348 1020
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2894 4378 4752 4898 387
Size: 1064 138 1305 1484 374 146 1280
Fragments arranged by size:
1484 1305 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2362 2362
Size: 5791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2972 3854 4451 5238 5506 1239
Size: 393 569 771 882 597 787 268 1524
Fragments arranged by size:
1524 882 787 771 597 569 393 268
EcoRI G'AATT_C
Cuts at: 3100 3478 3100
Size: 378 5413
FspI TGC'GCA
Cuts at: 4297 4297
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 2386 3064 4938 1678
Size: 708 678 1874 2531
Fragments arranged by size:
2531 1874 708 678
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2148 2180 2508 2540 4432
Size: 32 135 32 576 32 328 32 1892
Cuts at: 4432 4464 4506 4538 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1892 576 328 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2022 2503 3135 3381 669
Size: 988 281 84 481 632 246 3079
Fragments arranged by size:
3079 988 632 481 281 246 84
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5791
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KpnI G_GTAC'C
Cuts at: 3116 3116
Size: 5791
MfeI C'AATT_G
Cuts at: 2622 2763 3390 2622
Size: 141 627 5023
Fragments arranged by size:
5023 627 141
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2422 4905 5089 1305
Size: 1117 2483 184 2007
Fragments arranged by size:
2483 2007 1117 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3969
Fragments arranged by size:
3969 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2807 2869 3749 4108 4267 518
Size: 648 1641 62 880 359 159 2042
Fragments arranged by size:
2042 1641 880 648 359 159 62
MspA1I CmG'CkG
Cuts at: 1606 3207 3884 5032 5277 1606
Size: 1601 677 1148 245 2120
Fragments arranged by size:
2120 1601 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2706 513
Size: 552 939 702 3598
Fragments arranged by size:
3598 939 702 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 2033 2794 3149 4694 1132
Size: 97 804 761 355 1545 2229
Fragments arranged by size:
2229 1545 804 761 355 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5791
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2563 2593 1108
Size: 1455 30 4306
Fragments arranged by size:
4306 1455 30
PfoI T'CCnGG_A
Cuts at: 2489 2489
Size: 5791
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3002 3045 3361 3002
Size: 43 316 5432
Fragments arranged by size:
5432 316 43
PstI C_TGCA'G
Cuts at: 830 3143 830
Size: 2313 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
SacI G_AGCT'C
Cuts at: 2364 2364
Size: 5791
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SapI GCTCTTCn'nnn_
Cuts at: 2373 2373
Size: 5791
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SfcI C'TryA_G
Cuts at: 826 1045 3139 4274 4955 5146 826
Size: 219 2094 1135 681 191 1471
Fragments arranged by size:
2094 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 2641 1700
Size: 941 4850
SmaI CCC'GGG
Cuts at: 1872 3118 1872
Size: 1246 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2136 2437 3895 4796 5058 820
Size: 197 926 193 301 1458 901 262 1553
Fragments arranged by size:
1553 1458 926 901 301 262 197 193
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 3149 1132
Size: 2017 3774
SspI AAT'ATT
Cuts at: 5 52 2913 3715 5
Size: 47 2861 802 2081
Fragments arranged by size:
2861 2081 802 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5791
StyI C'CwwG_G
Cuts at: 513 1065 2004 2706 3230 513
Size: 552 939 702 524 3074
Fragments arranged by size:
3074 939 702 552 524
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3818 3835 3988 4173 4618 325
Size: 1255 2238 17 153 185 445 1498
Fragments arranged by size:
2238 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2248 4037 5749
Size: 274 80 33 51 1443 271 1789 1712
Cuts at: 5749 96
Size: 138
Fragments arranged by size:
1789 1712 1443 274 271 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5751 5783 5751
Size: 32 5759
Tth111I GACn'n_nGTC
Cuts at: 1316 2162 1316
Size: 846 4945
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 3116 1870
Size: 1246 4545
XmnI GAAnn'nnTTC
Cuts at: 3920 3920
Size: 5791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3599 276
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI