Restriction map for
pFN21AE3147
(Circular) (Six-base) MAPSORT of: pFN21AE3147.seq Check: 6191 from: 1 to: 5785
pFN21AE3147 5785 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2409 3595 278
Size: 53 83 186 936 873 1186 2468
Fragments arranged by size:
2468 1186 936 873 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 2675 3106 2675
Size: 431 5354
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3848 5232 5500 1632
Size: 2216 1384 268 1917
Fragments arranged by size:
2216 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 2072 4514 2072
Size: 2442 3343
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 3528 5100 3528
Size: 1572 4213
ApaI G_GGCC'C
Cuts at: 2122 2122
Size: 5785
ApaLI G'TGCA_C
Cuts at: 2656 3842 4998 2656
Size: 1186 1156 3443
Fragments arranged by size:
3443 1186 1156
ApoI r'AATT_y
Cuts at: 1493 2941 3094 3320 3472 5581 1493
Size: 1448 153 226 152 2109 1697
Fragments arranged by size:
2109 1697 1448 226 153 152
AseI AT'TA_AT
Cuts at: 160 1032 2205 4339 160
Size: 872 1173 2134 1606
Fragments arranged by size:
2134 1606 1173 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 3110 1870
Size: 73 1167 4545
Fragments arranged by size:
4545 1167 73
AvrII C'CTAG_G
Cuts at: 2425 2425
Size: 5785
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2675 3106 4561 618
Size: 325 217 627 888 431 1455 1842
Fragments arranged by size:
1842 1455 888 627 431 325 217
BanII G_rGCy'C
Cuts at: 1391 2032 2051 2122 3104 1391
Size: 641 19 71 982 4072
Fragments arranged by size:
4072 982 641 71 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2121 2458 2840 928
Size: 971 222 337 382 3873
Fragments arranged by size:
3873 971 382 337 222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2078 2112 3976 4010 1903
Size: 34 141 34 1864 34 3678
Fragments arranged by size:
3678 1864 141 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 2502 1548
Size: 954 4831
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2309 4396 136
Size: 107 122 71 631 605 637 2087 1525
Fragments arranged by size:
2087 1525 637 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2650 5780 2650
Size: 3130 2655
BlpI GC'TnA_GC
Cuts at: 3021 3202 3021
Size: 181 5604
Bme1580I G_kGCm'C
Cuts at: 1790 2122 2660 3846 5002 1790
Size: 332 538 1186 1156 2573
Fragments arranged by size:
2573 1186 1156 538 332
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3516 4474 442
Size: 416 53 83 399 24 2099 958 1753
Fragments arranged by size:
2099 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 2735 4445 1239
Size: 760 736 1710 2579
Fragments arranged by size:
2579 1710 760 736
Bpu10I CC'TnA_GC
Cuts at: 2904 2904
Size: 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2794 3910 4775 5073 2794
Size: 1116 865 298 3506
Fragments arranged by size:
3506 1116 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4448 882
Size: 617 2949 2219
Fragments arranged by size:
2949 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2764 3576 493
Size: 711 1560 812 2702
Fragments arranged by size:
2702 1560 812 711
BsaBI GATnn'nnATC
Cuts at: 2550 5779 2550
Size: 3229 2556
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2406 2641 3592 3974
Size: 53 83 186 936 873 235 951 382
Cuts at: 3974 4609 275
Size: 635 1451
Fragments arranged by size:
1451 951 936 873 635 382 235 186
83 53
BsaWI w'CCGG_w
Cuts at: 2562 2634 4218 4616 4890 5037 5448 2562
Size: 72 1584 398 274 147 411 2899
Fragments arranged by size:
2899 1584 411 398 274 147 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5560
Fragments arranged by size:
5560 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2799 1186
Size: 1613 4172
BseYI C'CCAG_C
Cuts at: 2025 2683 2881 2980 4988 5484 2025
Size: 658 198 99 2008 496 2326
Fragments arranged by size:
2326 2008 658 496 198 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3023 1163
Size: 573 1287 3925
Fragments arranged by size:
3925 1287 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2036 3235 3996 5024 1540
Size: 275 186 35 1199 761 1028 2301
Fragments arranged by size:
2301 1199 1028 761 275 186 35
BsiHKAI G_wGCw'C
Cuts at: 2660 3846 3931 5002 2660
Size: 1186 85 1071 3443
Fragments arranged by size:
3443 1186 1071 85
BsmI GAATG_Cn'
Cuts at: 3296 3389 3296
Size: 93 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2051 2122 2660 3104 3846 3931
Size: 399 242 19 71 538 444 742 85
Cuts at: 3931 5002 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 538 444 399 242 85
71 19
BspEI T'CCGG_A
Cuts at: 2562 5448 2562
Size: 2886 2899
BspHI T'CATG_A
Cuts at: 2271 3674 5404 2271
Size: 1403 1730 2652
Fragments arranged by size:
2652 1730 1403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2811 3140 844
Size: 1967 329 3489
Fragments arranged by size:
3489 1967 329
BsrBI CCG'CTC
Cuts at: 1394 1823 3672 1394
Size: 429 1849 3507
Fragments arranged by size:
3507 1849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4280 4454 65
Size: 1183 3032 174 1396
Fragments arranged by size:
3032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 2259 96
Size: 2163 3622
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 2185 1184
Size: 1001 4784
BstXI CCAn_nnnn'nTGG
Cuts at: 2461 2461
Size: 5785
BstYI r'GATC_y
Cuts at: 1972 2650 3115 3482 3867 3884 5336 5422 5434
Size: 678 465 367 385 17 1452 86 12
Cuts at: 5434 5780 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 678 465 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2932 513
Size: 552 939 928 3366
Fragments arranged by size:
3366 939 928 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5046
Fragments arranged by size:
5046 683 56
BtsI GCAGTG_nn'
Cuts at: 3301 4095 4115 3301
Size: 794 20 4971
Fragments arranged by size:
4971 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DraIII CAC_nnn'GTG
Cuts at: 2415 3046 2415
Size: 631 5154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2890 3232 4121
Size: 54 1425 50 275 18 1060 342 889
Cuts at: 4121 5750 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1060 889 342 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3715 1353
Size: 2362 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2387 4372 4746 4892 387
Size: 1064 138 798 1985 374 146 1280
Fragments arranged by size:
1985 1280 1064 798 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2735 3848 4445 5232 5500 1239
Size: 393 367 736 1113 597 787 268 1524
Fragments arranged by size:
1524 1113 787 736 597 393 367 268
EcoO109I rG'GnC_Cy
Cuts at: 2118 2119 2118
Size: 1 5784
EcoRI G'AATT_C
Cuts at: 2941 3094 3472 2941
Size: 153 378 5254
Fragments arranged by size:
5254 378 153
EcoRV GAT'ATC
Cuts at: 2548 2698 2548
Size: 150 5635
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2012 4932 1678
Size: 334 2920 2531
Fragments arranged by size:
2920 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 4426 4458 4500
Size: 32 135 32 486 32 2336 32 42
Cuts at: 4500 4532 1373
Size: 32 2626
Fragments arranged by size:
2626 2336 486 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3129 3375 669
Size: 988 281 1191 246 3079
Fragments arranged by size:
3079 1191 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2135 748
Size: 1387 4398
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 2679 3110 2679
Size: 431 5354
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4899 5083 1305
Size: 3594 184 2007
Fragments arranged by size:
3594 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2892 10
Size: 54 1425 343 1060 2903
Fragments arranged by size:
2903 1425 1060 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2955 3743 4102 4261 518
Size: 648 1789 788 359 159 2042
Fragments arranged by size:
2042 1789 788 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2896 3201 3878 5026 5271 1606
Size: 1290 305 677 1148 245 2120
Fragments arranged by size:
2120 1290 1148 677 305 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 3143 4688 1132
Size: 97 1914 1545 2229
Fragments arranged by size:
2229 1914 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2734 1108
Size: 1626 4159
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PmlI CAC'GTG
Cuts at: 2764 2764
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PspOMI G'GGCC_C
Cuts at: 2118 2118
Size: 5785
PstI C_TGCA'G
Cuts at: 830 2997 3008 3137 830
Size: 2167 11 129 3478
Fragments arranged by size:
3478 2167 129 11
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2896 2896
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SexAI A'CCwGG_T
Cuts at: 2460 2460
Size: 5785
SfcI C'TryA_G
Cuts at: 826 1045 2993 3004 3133 4268 4949 5140 826
Size: 219 1948 11 129 1135 681 191 1471
Fragments arranged by size:
1948 1471 1135 681 219 191 129 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2773 3889 4790 5052 820
Size: 197 926 830 1116 901 262 1553
Fragments arranged by size:
1553 1116 926 901 830 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 2004 2425 3070 3224 513
Size: 552 939 421 645 154 3074
Fragments arranged by size:
3074 939 645 552 421 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2022 2115 2592 2697 3812 3829 3982
Size: 1255 442 93 477 105 1115 17 153
Cuts at: 3982 4167 4612 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1115 477 445 442 185 153
105 93 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 4031 5743
Size: 274 80 33 51 1443 282 1772 1712
Cuts at: 5743 96
Size: 138
Fragments arranged by size:
1772 1712 1443 282 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2407 3593 276
Size: 53 83 186 936 873 1186 2468
Fragments arranged by size:
2468 1186 936 873 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI