Restriction map for
pFN21AE3144
(Circular) (Six-base) MAPSORT of: pFN21AE3144.seq Check: 3373 from: 1 to: 5767
pFN21AE3144 5767 bp
With 182 enzymes: SgfI *
March 28, 2012 13:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2305 3110 1937
Size: 368 805 4594
Fragments arranged by size:
4594 805 368
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3830 5214 5482 1632
Size: 2198 1384 268 1917
Fragments arranged by size:
2198 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 4666 5643 1203
Size: 3463 977 1327
Fragments arranged by size:
3463 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 2935 4496 2935
Size: 1561 4206
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AlwNI CAG_nnn'CTG
Cuts at: 2222 2650 3510 5082 2222
Size: 428 860 1572 2907
Fragments arranged by size:
2907 1572 860 428
ApaI G_GGCC'C
Cuts at: 2060 2177 2060
Size: 117 5650
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2176 2201 2493 3092 1870
Size: 73 117 116 25 292 599 4545
Fragments arranged by size:
4545 599 292 117 116 73 25
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 3088 4543 618
Size: 325 217 627 255 1046 1455 1842
Fragments arranged by size:
1842 1455 1046 627 325 255 217
BanII G_rGCy'C
Cuts at: 1391 2060 2177 2208 2609 2682 3086 1391
Size: 669 117 31 401 73 404 4072
Fragments arranged by size:
4072 669 404 401 117 73 31
BbeI G_GCGC'C
Cuts at: 2046 2046
Size: 5767
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4796
BbvCI CC'TCA_GC
Cuts at: 2215 2215
Size: 5767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3958 3992 1903
Size: 34 2021 34 3678
Fragments arranged by size:
3678 2021 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2744 3659 4869 2744
Size: 915 1210 3642
Fragments arranged by size:
3642 1210 915
BclI T'GATC_A
Cuts at: 1548 2406 1548
Size: 858 4909
BfrBI ATG'CAT
Cuts at: 2402 2402
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 2231 4378
Size: 107 122 71 631 605 379 180 2147
Cuts at: 4378 136
Size: 1525
Fragments arranged by size:
2147 1525 631 605 379 180 122 107
71
BglII A'GATC_T
Cuts at: 5762 5762
Size: 5767
BlpI GC'TnA_GC
Cuts at: 2243 2264 3184 2243
Size: 21 920 4826
Fragments arranged by size:
4826 920 21
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2177 3828 4984 1790
Size: 270 117 1651 1156 2573
Fragments arranged by size:
2573 1651 1156 270 117
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2260 2284 3498
Size: 416 53 83 399 24 843 24 1214
Cuts at: 3498 4456 442
Size: 958 1753
Fragments arranged by size:
1753 1214 958 843 416 399 83 53
24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2337 2623 2852 2999 4427 1239
Size: 1098 286 229 147 1428 2579
Fragments arranged by size:
2579 1428 1098 286 229 147
Bpu10I CC'TnA_GC
Cuts at: 2215 2215
Size: 5767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2590 3892 4757 5055 2590
Size: 1302 865 298 3302
Fragments arranged by size:
3302 1302 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2738 4430 882
Size: 617 1239 1692 2219
Fragments arranged by size:
2219 1692 1239 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 3574 3956 4591
Size: 53 83 186 936 510 1531 382 635
Cuts at: 4591 275
Size: 1451
Fragments arranged by size:
1531 1451 936 635 510 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2310 4200 4598 4872 5019 5430 2310
Size: 1890 398 274 147 411 2647
Fragments arranged by size:
2647 1890 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2340 2614 1186
Size: 853 301 274 4339
Fragments arranged by size:
4339 853 301 274
BseYI C'CCAG_C
Cuts at: 2608 4970 5466 2608
Size: 2362 496 2909
Fragments arranged by size:
2909 2362 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2858 1163
Size: 573 1122 4072
Fragments arranged by size:
4072 1122 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3217 3978 5006 1540
Size: 275 186 1216 761 1028 2301
Fragments arranged by size:
2301 1216 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2634 3828 3913 4984 2634
Size: 1194 85 1071 3417
Fragments arranged by size:
3417 1194 1071 85
BsmI GAATG_Cn'
Cuts at: 3004 3278 3371 3004
Size: 274 93 5400
Fragments arranged by size:
5400 274 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2177 2208 2609 2634 2682 3086
Size: 399 270 117 31 401 25 48 404
Cuts at: 3086 3828 3913 4984 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 404 401 399 270 117
85 48 31 25
BspEI T'CCGG_A
Cuts at: 2310 5430 2310
Size: 3120 2647
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2605 3122 844
Size: 1761 517 3489
Fragments arranged by size:
3489 1761 517
BsrBI CCG'CTC
Cuts at: 1394 1823 2184 3654 1394
Size: 429 361 1470 3507
Fragments arranged by size:
3507 1470 429 361
BsrDI GCAATG_nn'
Cuts at: 65 1248 2560 4262 4436 65
Size: 1183 1312 1702 174 1396
Fragments arranged by size:
1702 1396 1312 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4411 4598 1700
Size: 110 2601 187 2869
Fragments arranged by size:
2869 2601 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 2741 3827 4839 2741
Size: 1086 1012 3669
Fragments arranged by size:
3669 1086 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5767
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 3097 3464 3849 3866 5318 5404 5416 5762
Size: 1125 367 385 17 1452 86 12 346
Cuts at: 5762 1972
Size: 1977
Fragments arranged by size:
1977 1452 1125 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3059 3059
Size: 5767
BtgI C'CryG_G
Cuts at: 513 1065 2004 2227 513
Size: 552 939 223 4053
Fragments arranged by size:
4053 939 552 223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 2306 3283 4077 4097 2306
Size: 977 794 20 3976
Fragments arranged by size:
3976 977 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2964 4774 809
Size: 507 1648 1810 1802
Fragments arranged by size:
1810 1802 1648 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3214 4103 5732
Size: 54 1425 50 275 18 1384 889 1629
Cuts at: 5732 8
Size: 43
Fragments arranged by size:
1629 1425 1384 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3697 1353
Size: 2344 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2457 4354 4728 4874 387
Size: 1064 138 435 433 1897 374 146 1280
Fragments arranged by size:
1897 1280 1064 435 433 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2337 2623 2852 2999 3830 4427 5214
Size: 393 705 286 229 147 831 597 787
Cuts at: 5214 5482 1239
Size: 268 1524
Fragments arranged by size:
1524 831 787 705 597 393 286 268
229 147
EcoNI CCTnn'n_nnAGG
Cuts at: 2352 2433 2352
Size: 81 5686
EcoO109I rG'GnC_Cy
Cuts at: 2057 2173 2254 2899 2057
Size: 116 81 645 4925
Fragments arranged by size:
4925 645 116 81
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
EcoRV GAT'ATC
Cuts at: 2326 2326
Size: 5767
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2046 4914 1678
Size: 368 2868 2531
Fragments arranged by size:
2868 2531 368
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2745 2777 2921
Size: 32 135 32 743 32 398 32 144
Cuts at: 2921 2953 4408 4440 4482 4514 1373
Size: 32 1455 32 42 32 2626
Fragments arranged by size:
2626 1455 743 398 144 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2383 3111 3357 669
Size: 988 281 445 728 246 3079
Fragments arranged by size:
3079 988 728 445 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KasI G'GCGC_C
Cuts at: 2042 2042
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2042 2295 4881 5065 1305
Size: 737 253 2586 184 2007
Fragments arranged by size:
2586 2007 737 253 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3945
Fragments arranged by size:
3945 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3725 4084 4243 518
Size: 648 2559 359 159 2042
Fragments arranged by size:
2559 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2103 2159 2197 2222 3183 3860 5008 5253
Size: 497 56 38 25 961 677 1148 245
Cuts at: 5253 1606
Size: 2120
Fragments arranged by size:
2120 1148 961 677 497 245 56 38
25
NarI GG'CG_CC
Cuts at: 2043 2043
Size: 5767
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4276
Fragments arranged by size:
4276 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NsiI A_TGCA'T
Cuts at: 2404 2404
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 3125 4670 1132
Size: 97 1896 1545 2229
Fragments arranged by size:
2229 1896 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 4666 4666
Size: 5767
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5767
PfoI T'CCnGG_A
Cuts at: 2353 2704 2353
Size: 351 5416
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 2899 2254
Size: 645 5122
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 2340 3337 2340
Size: 997 4770
PspOMI G'GGCC_C
Cuts at: 2056 2173 2056
Size: 117 5650
PstI C_TGCA'G
Cuts at: 830 3119 830
Size: 2289 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SanDI GG'GwC_CC
Cuts at: 2254 2254
Size: 5767
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 3115 4250 4931 5122 826
Size: 219 2070 1135 681 191 1471
Fragments arranged by size:
2070 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2044 2044
Size: 5767
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 2062 2178 2203 3094 1872
Size: 190 116 25 891 4545
Fragments arranged by size:
4545 891 190 116 25
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2569 3871 4772 5034 820
Size: 197 926 626 1302 901 262 1553
Fragments arranged by size:
1553 1302 926 901 626 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SrfI GCCC'GGGC
Cuts at: 2203 2203
Size: 5767
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 2004 3206 513
Size: 552 939 1202 3074
Fragments arranged by size:
3074 1202 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2502 3794 3811 3964 4149 4594 325
Size: 1255 922 1292 17 153 185 445 1498
Fragments arranged by size:
1498 1292 1255 922 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4013 5725 96
Size: 274 80 33 51 1443 2036 1712 138
Fragments arranged by size:
2036 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2768 2800 5727 5759 2768
Size: 32 2927 32 2776
Fragments arranged by size:
2927 2776 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5767
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 2060 2176 2201 3092 1870
Size: 190 116 25 891 4545
Fragments arranged by size:
4545 891 190 116 25
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI
NaeI NgoMIV NruI PacI PmlI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SwaI XcmI