Restriction map for
pFN21AE3143
(Circular) (Six-base) MAPSORT of: pFN21AE3143.seq Check: 4063 from: 1 to: 5764
pFN21AE3143 5764 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2243 2435 2243
Size: 192 5572
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 1937
Size: 1170 4594
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3827 5211 5479 1632
Size: 2195 1384 268 1917
Fragments arranged by size:
2195 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 2411 2646 4663 5640 1203
Size: 1208 235 2017 977 1327
Fragments arranged by size:
2017 1327 1208 977 235
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5764
AlwNI CAG_nnn'CTG
Cuts at: 2803 3507 5079 2803
Size: 704 1572 3488
Fragments arranged by size:
3488 1572 704
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 2999 3073 3299 3451 5560 1493
Size: 1506 74 226 152 2109 1697
Fragments arranged by size:
2109 1697 1506 226 152 74
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 3089 1870
Size: 73 1146 4545
Fragments arranged by size:
4545 1146 73
BamHI G'GATC_C
Cuts at: 2451 3094 3461 2451
Size: 643 367 4754
Fragments arranged by size:
4754 643 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2419 3085 4540 618
Size: 325 217 627 632 666 1455 1842
Fragments arranged by size:
1842 1455 666 632 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3083 1391
Size: 1692 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2482 2900 928
Size: 971 583 418 3792
Fragments arranged by size:
3792 971 583 418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3955 3989 1903
Size: 34 2018 34 3678
Fragments arranged by size:
3678 2018 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 2250 1548
Size: 702 5062
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4375 136
Size: 107 122 71 631 605 2703 1525
Fragments arranged by size:
2703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 2630 2720 3181 2630
Size: 90 461 5213
Fragments arranged by size:
5213 461 90
Bme1580I G_kGCm'C
Cuts at: 1790 2424 3825 4981 1790
Size: 634 1401 1156 2573
Fragments arranged by size:
2573 1401 1156 634
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3495 4453 442
Size: 416 53 83 399 24 2078 958 1753
Fragments arranged by size:
2078 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2232 2317 2597 4424 1239
Size: 866 127 85 280 1827 2579
Fragments arranged by size:
2579 1827 866 280 127 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3889 4754 5052 3889
Size: 865 298 4601
Fragments arranged by size:
4601 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4427 882
Size: 617 2928 2219
Fragments arranged by size:
2928 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2412 3555 493
Size: 711 1208 1143 2702
Fragments arranged by size:
2702 1208 1143 711
BsaBI GATnn'nnATC
Cuts at: 2037 5758 2037
Size: 3721 2043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2955 3571 3953 4588
Size: 53 83 186 936 1422 616 382 635
Cuts at: 4588 275
Size: 1451
Fragments arranged by size:
1451 1422 936 635 616 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4197 4595 4869 5016 5427 4197
Size: 398 274 147 411 4534
Fragments arranged by size:
4534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2510 2540 1178
Size: 30 165 30 1107 30 4402
Fragments arranged by size:
4402 1107 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5764
BseYI C'CCAG_C
Cuts at: 2887 4967 5463 2887
Size: 2080 496 3188
Fragments arranged by size:
3188 2080 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2576 1163
Size: 573 840 4351
Fragments arranged by size:
4351 840 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2342 3214 3975 5003 1540
Size: 275 186 341 872 761 1028 2301
Fragments arranged by size:
2301 1028 872 761 341 275 186
BsiHKAI G_wGCw'C
Cuts at: 2333 2379 2946 3825 3910 4981 2333
Size: 46 567 879 85 1071 3116
Fragments arranged by size:
3116 1071 879 567 85 46
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 2379 2424 2946 3083 3825 3910
Size: 399 543 46 45 522 137 742 85
Cuts at: 3910 4981 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 543 522 399 137 85
46 45
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 3653 5383 3653
Size: 1730 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2243 2298 2435 3119 844
Size: 1399 55 137 684 3489
Fragments arranged by size:
3489 1399 684 137 55
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 1394
Size: 429 1828 3507
Fragments arranged by size:
3507 1828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2477 4259 4433 65
Size: 1183 1229 1782 174 1396
Fragments arranged by size:
1782 1396 1229 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4408 4595 1700
Size: 110 2598 187 2869
Fragments arranged by size:
2869 2598 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 2243 3824 4836 2243
Size: 1581 1012 3171
Fragments arranged by size:
3171 1581 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5764
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5764
BstXI CCAn_nnnn'nTGG
Cuts at: 2104 2536 2779 2104
Size: 432 243 5089
Fragments arranged by size:
5089 432 243
BstYI r'GATC_y
Cuts at: 1972 2451 3094 3461 3846 3863 5315 5401 5413
Size: 479 643 367 385 17 1452 86 12
Cuts at: 5413 5759 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 643 479 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 513
Size: 552 939 39 4234
Fragments arranged by size:
4234 939 552 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5025
Fragments arranged by size:
5025 683 56
BtsI GCAGTG_nn'
Cuts at: 3280 4074 4094 3280
Size: 794 20 4950
Fragments arranged by size:
4950 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2763 2798 529
Size: 35 2199 35 3495
Fragments arranged by size:
3495 2199 35 35
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DraIII CAC_nnn'GTG
Cuts at: 2248 2269 2248
Size: 21 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2339 3211 4100
Size: 54 1425 50 275 18 509 872 889
Cuts at: 4100 5729 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 872 509 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2339 3211 1537
Size: 275 527 872 4090
Fragments arranged by size:
4090 872 527 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2619 2985 3018 3024 3694 1353
Size: 1266 366 33 6 670 3423
Fragments arranged by size:
3423 1266 670 366 33 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2232 2317 2597 3827 4424 5211
Size: 393 473 127 85 280 1230 597 787
Cuts at: 5211 5479 1239
Size: 268 1524
Fragments arranged by size:
1524 1230 787 597 473 393 280 268
127 85
EcoNI CCTnn'n_nnAGG
Cuts at: 2027 2027
Size: 5764
EcoO109I rG'GnC_Cy
Cuts at: 2734 2734
Size: 5764
EcoRI G'AATT_C
Cuts at: 2999 3073 3451 2999
Size: 74 378 5312
Fragments arranged by size:
5312 378 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 5764
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 4911 1678
Size: 3233 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2242 2274 3017
Size: 32 135 32 428 32 210 32 743
Cuts at: 3017 3049 4405 4437 4479 4511 1373
Size: 32 1356 32 42 32 2626
Fragments arranged by size:
2626 1356 743 428 210 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3108 3354 669
Size: 988 281 1170 246 3079
Fragments arranged by size:
3079 1170 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2717 2868 748
Size: 1969 151 3644
Fragments arranged by size:
3644 1969 151
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4878 5062 1305
Size: 3573 184 2007
Fragments arranged by size:
3573 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3942
Fragments arranged by size:
3942 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2382 2534 3722 4081 4240 518
Size: 648 1216 152 1188 359 159 2042
Fragments arranged by size:
2042 1216 1188 648 359 159 152
MspA1I CmG'CkG
Cuts at: 1606 2065 2226 2344 2575 2692 2761 2887 3180
Size: 459 161 118 231 117 69 126 293
Cuts at: 3180 3857 5005 5250 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 459 293 245 231 161
126 118 117 69
NcoI C'CATG_G
Cuts at: 513 1065 2004 2043 513
Size: 552 939 39 4234
Fragments arranged by size:
4234 939 552 39
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 3122 4667 1132
Size: 97 1893 1545 2229
Fragments arranged by size:
2229 1893 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2803 1108
Size: 1695 4069
PfoI T'CCnGG_A
Cuts at: 2028 2661 2028
Size: 633 5131
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PmlI CAC'GTG
Cuts at: 2412 2412
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5764
PsiI TTA'TAA
Cuts at: 2013 3334 2013
Size: 1321 4443
PstI C_TGCA'G
Cuts at: 830 2283 2349 2691 3116 830
Size: 1453 66 342 425 3478
Fragments arranged by size:
3478 1453 425 342 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
PvuII CAG'CTG
Cuts at: 2065 2575 2761 2887 2065
Size: 510 186 126 4942
Fragments arranged by size:
4942 510 186 126
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SapI GCTCTTCn'nnn_
Cuts at: 2619 2619
Size: 5764
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SexAI A'CCwGG_T
Cuts at: 2613 3060 2613
Size: 447 5317
SfcI C'TryA_G
Cuts at: 826 1045 2279 2345 2687 3112 4247 4928 5119
Size: 219 1234 66 342 425 1135 681 191
Cuts at: 5119 826
Size: 1471
Fragments arranged by size:
1471 1234 1135 681 425 342 219 191
66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 3091 1872
Size: 1219 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3868 4769 5031 820
Size: 197 926 1925 901 262 1553
Fragments arranged by size:
1925 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 2462 1517
Size: 945 4819
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 2598 2874 3203 513
Size: 552 939 39 555 276 329 3074
Fragments arranged by size:
3074 939 555 552 329 276 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3791 3808 3961 4146 4591 325
Size: 1255 2211 17 153 185 445 1498
Fragments arranged by size:
2211 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4010 5722 96
Size: 274 80 33 51 1443 2033 1712 138
Fragments arranged by size:
2033 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2536 2568 5724 5756 2536
Size: 32 3156 32 2544
Fragments arranged by size:
3156 2544 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 2779 2800 2050
Size: 729 21 5014
Fragments arranged by size:
5014 729 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 3089 1870
Size: 1219 4545
XmnI GAAnn'nnTTC
Cuts at: 3893 3893
Size: 5764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI