Restriction map for
pFN21AE3140
(Circular) (Six-base) MAPSORT of: pFN21AE3140.seq Check: 9490 from: 1 to: 5755
pFN21AE3140 5755 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2463 3098 1937
Size: 526 635 4594
Fragments arranged by size:
4594 635 526
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3818 5202 5470 1632
Size: 2186 1384 268 1917
Fragments arranged by size:
2186 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 2769 3498 5070 2769
Size: 729 1572 3454
Fragments arranged by size:
3454 1572 729
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2347 3064 3290 3442 5551 1493
Size: 854 717 226 152 2109 1697
Fragments arranged by size:
2109 1697 854 717 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2478 3080 1870
Size: 73 127 408 602 4545
Fragments arranged by size:
4545 602 408 127 73
BamHI G'GATC_C
Cuts at: 2406 3085 3452 2406
Size: 679 367 4709
Fragments arranged by size:
4709 679 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2324 3076 4531 618
Size: 325 217 627 537 752 1455 1842
Fragments arranged by size:
1842 1455 752 627 537 325 217
BanII G_rGCy'C
Cuts at: 1391 2079 2479 3074 1391
Size: 688 400 595 4072
Fragments arranged by size:
4072 688 595 400
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2807 2856 928
Size: 971 908 49 3827
Fragments arranged by size:
3827 971 908 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3946 3980 1903
Size: 34 50 34 1925 34 3678
Fragments arranged by size:
3678 1925 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2603 3647 4857 2603
Size: 1044 1210 3501
Fragments arranged by size:
3501 1210 1044
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2160 2312 4366
Size: 107 122 71 631 605 488 152 2054
Cuts at: 4366 136
Size: 1525
Fragments arranged by size:
2054 1525 631 605 488 152 122 107
71
BglII A'GATC_T
Cuts at: 2400 5750 2400
Size: 3350 2405
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2455 3816 4972 1790
Size: 665 1361 1156 2573
Fragments arranged by size:
2573 1361 1156 665
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2597 4415 1239
Size: 911 447 1818 2579
Fragments arranged by size:
2579 1818 911 447
Bpu10I CC'TnA_GC
Cuts at: 2974 2974
Size: 5755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2170 2276 2853 3880 4745 5043 2170
Size: 106 577 1027 865 298 2882
Fragments arranged by size:
2882 1027 865 577 298 106
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2670 4418 882
Size: 617 1171 1748 2219
Fragments arranged by size:
2219 1748 1171 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 2405 5749 2405
Size: 3344 2411
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3562 3944 4579 275
Size: 53 83 186 936 2029 382 635 1451
Fragments arranged by size:
2029 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2601 4188 4586 4860 5007 5418 2601
Size: 1587 398 274 147 411 2938
Fragments arranged by size:
2938 1587 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2894 1186
Size: 1708 4047
BseYI C'CCAG_C
Cuts at: 2227 2980 4958 5454 2227
Size: 753 1978 496 2528
Fragments arranged by size:
2528 1978 753 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5182
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2752 3816 3901 4972 2752
Size: 1064 85 1071 3535
Fragments arranged by size:
3535 1071 1064 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2455 2479 2752 3074 3816 3901
Size: 399 289 376 24 273 322 742 85
Cuts at: 3901 4972 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 376 322 289 273
85 24
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2454 2813 3110 844
Size: 1610 359 297 3489
Fragments arranged by size:
3489 1610 359 297
BsrBI CCG'CTC
Cuts at: 1394 1823 2060 3642 1394
Size: 429 237 1582 3507
Fragments arranged by size:
3507 1582 429 237
BsrDI GCAATG_nn'
Cuts at: 65 1248 2211 4250 4424 65
Size: 1183 963 2039 174 1396
Fragments arranged by size:
2039 1396 1183 963 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 2266 4559 2266
Size: 2293 3462
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstXI CCAn_nnnn'nTGG
Cuts at: 2315 2704 2315
Size: 389 5366
BstYI r'GATC_y
Cuts at: 1972 2400 2406 3013 3085 3452 3837 3854 5306
Size: 428 6 607 72 367 385 17 1452
Cuts at: 5306 5392 5404 5750 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 607 428 385 367 346 86
72 17 12 6
BtgI C'CryG_G
Cuts at: 513 1065 2565 513
Size: 552 1500 3703
Fragments arranged by size:
3703 1500 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 3271 4065 4085 3271
Size: 794 20 4941
Fragments arranged by size:
4941 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2947 2982 529
Size: 35 2383 35 3302
Fragments arranged by size:
3302 2383 35 35
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DraIII CAC_nnn'GTG
Cuts at: 2344 2344
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2294 4762 809
Size: 507 978 2468 1802
Fragments arranged by size:
2468 1802 978 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2562 3202
Size: 54 1425 50 275 18 210 522 640
Cuts at: 3202 4091 5720 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 640 522 275 210 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2958 3040 3685 1353
Size: 1605 82 645 3423
Fragments arranged by size:
3423 1605 645 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2597 3818 4415 5202 5470 1239
Size: 393 518 447 1221 597 787 268 1524
Fragments arranged by size:
1524 1221 787 597 518 447 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2180 2535 2920 2180
Size: 355 385 5015
Fragments arranged by size:
5015 385 355
EcoO109I rG'GnC_Cy
Cuts at: 2161 2296 2837 2925 2161
Size: 135 541 88 4991
Fragments arranged by size:
4991 541 135 88
EcoRI G'AATT_C
Cuts at: 2347 3064 3442 2347
Size: 717 378 4660
Fragments arranged by size:
4660 717 378
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 4902 1678
Size: 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4396 4428 4470 4502 1373
Size: 32 135 32 2824 32 42 32 2626
Fragments arranged by size:
2824 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2564 10
Size: 54 1425 343 732 3201
Fragments arranged by size:
3201 1425 732 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3713 4072 4231 518
Size: 648 2547 359 159 2042
Fragments arranged by size:
2547 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2330 2617 2769 3171 3848 4996 5241
Size: 439 285 287 152 402 677 1148 245
Cuts at: 5241 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 439 402 287 285 245
152
NcoI C'CATG_G
Cuts at: 513 1065 2565 513
Size: 552 1500 3703
Fragments arranged by size:
3703 1500 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 3113 4658 1132
Size: 97 1884 1545 2229
Fragments arranged by size:
2229 1884 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5755
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2296 2837 2925 2296
Size: 541 88 5126
Fragments arranged by size:
5126 541 88
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PstI C_TGCA'G
Cuts at: 830 2017 2207 3107 830
Size: 1187 190 900 3478
Fragments arranged by size:
3478 1187 900 190
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 2013 2203 2331 3103 4238 4919 5110
Size: 219 968 190 128 772 1135 681 191
Cuts at: 5110 826
Size: 1471
Fragments arranged by size:
1471 1135 968 772 681 219 191 190
128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 2072 3082 1872
Size: 200 1010 4545
Fragments arranged by size:
4545 1010 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2149 2291 2868 3859 4760 5022
Size: 197 926 206 142 577 991 901 262
Cuts at: 5022 820
Size: 1553
Fragments arranged by size:
1553 991 926 901 577 262 206 197
142
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2511 2565 2900 3194 513
Size: 552 1446 54 335 294 3074
Fragments arranged by size:
3074 1446 552 335 294 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2205 3782 3799 3952 4137 4582 325
Size: 1255 625 1577 17 153 185 445 1498
Fragments arranged by size:
1577 1498 1255 625 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2335 4001 5713
Size: 274 80 33 51 1443 358 1666 1712
Cuts at: 5713 96
Size: 138
Fragments arranged by size:
1712 1666 1443 358 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 2070 3080 1870
Size: 200 1010 4545
Fragments arranged by size:
4545 1010 200
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI