Restriction map for
pFN21AE3139
(Circular) (Six-base) MAPSORT of: pFN21AE3139.seq Check: 2847 from: 1 to: 5755
pFN21AE3139 5755 bp
With 182 enzymes: SgfI *
March 28, 2012 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2780 2811 3565 278
Size: 53 83 186 936 1244 31 754 2468
Fragments arranged by size:
2468 1244 936 754 186 83 53 31
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3818 5202 5470 1632
Size: 2186 1384 268 1917
Fragments arranged by size:
2186 1917 1384 268
AfeI AGC'GCT
Cuts at: 2606 2606
Size: 5755
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 2220 4654 5631 1203
Size: 1017 2434 977 1327
Fragments arranged by size:
2434 1327 1017 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2240 1184
Size: 1056 4699
AlwNI CAG_nnn'CTG
Cuts at: 2380 2751 2847 3498 5070 2380
Size: 371 96 651 1572 3065
Fragments arranged by size:
3065 1572 651 371 96
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2990 3064 3290 3442 5551 1493
Size: 1497 74 226 152 2109 1697
Fragments arranged by size:
2109 1697 1497 226 152 74
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2192 2485 2915 3080 1870
Size: 73 249 293 430 165 4545
Fragments arranged by size:
4545 430 293 249 165 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2380 2413 2380
Size: 33 5722
BamHI G'GATC_C
Cuts at: 2625 3085 3452 2625
Size: 460 367 4928
Fragments arranged by size:
4928 460 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 3076 4531 618
Size: 325 217 627 324 965 1455 1842
Fragments arranged by size:
1842 1455 965 627 325 324 217
BanII G_rGCy'C
Cuts at: 1391 2922 3074 1391
Size: 1531 152 4072
Fragments arranged by size:
4072 1531 152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2803 2989 928
Size: 971 904 186 3694
Fragments arranged by size:
3694 971 904 186
BbvCI CC'TCA_GC
Cuts at: 2025 2840 2025
Size: 815 4940
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3946 3980 1903
Size: 34 2009 34 3678
Fragments arranged by size:
3678 2009 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2408 3647 4857 2408
Size: 1239 1210 3306
Fragments arranged by size:
3306 1239 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4366 136
Size: 107 122 71 631 605 337 2357 1525
Fragments arranged by size:
2357 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2946 5750 2946
Size: 2804 2951
BlpI GC'TnA_GC
Cuts at: 2672 3172 2672
Size: 500 5255
Bme1580I G_kGCm'C
Cuts at: 1790 2345 2499 3816 4972 1790
Size: 555 154 1317 1156 2573
Fragments arranged by size:
2573 1317 1156 555 154
BmgBI CAC'GTC
Cuts at: 1318 2512 2938 2964 1318
Size: 1194 426 26 4109
Fragments arranged by size:
4109 1194 426 26
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2437 2053
Size: 384 5371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2023 2489 4415 1239
Size: 784 466 1926 2579
Fragments arranged by size:
2579 1926 784 466
Bpu10I CC'TnA_GC
Cuts at: 2025 2840 2025
Size: 815 4940
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3880 4745 5043 3880
Size: 865 298 4592
Fragments arranged by size:
4592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2494 3546 493
Size: 711 1290 1052 2702
Fragments arranged by size:
2702 1290 1052 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2301 2777 2808 2886
Size: 53 83 186 936 768 476 31 78
Cuts at: 2886 3562 3944 4579 275
Size: 676 382 635 1451
Fragments arranged by size:
1451 936 768 676 635 476 382 186
83 78 53 31
BsaWI w'CCGG_w
Cuts at: 2393 2580 4188 4586 4860 5007 5418 2393
Size: 187 1608 398 274 147 411 2730
Fragments arranged by size:
2730 1608 411 398 274 187 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 1178
Size: 30 165 30 1041 30 4459
Fragments arranged by size:
4459 1041 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2351 1186
Size: 856 309 4590
Fragments arranged by size:
4590 856 309
BseYI C'CCAG_C
Cuts at: 2012 4958 5454 2012
Size: 2946 496 2313
Fragments arranged by size:
2946 2313 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2271 2717 2927 1163
Size: 573 535 446 210 3991
Fragments arranged by size:
3991 573 535 446 210
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2135 2790 2922 3816 3901 4972 2135
Size: 655 132 894 85 1071 2918
Fragments arranged by size:
2918 1071 894 655 132 85
BsiWI C'GTAC_G
Cuts at: 2245 2245
Size: 5755
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
BsmBI CGTCTCn'nnnn_
Cuts at: 2543 2543
Size: 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2345 2499 2790 2922 3074 3816
Size: 399 345 210 154 291 132 152 742
Cuts at: 3816 3901 4972 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 345 291 210 154
152 132 85
BspEI T'CCGG_A
Cuts at: 2580 5418 2580
Size: 2838 2917
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2657 3110 844
Size: 1813 453 3489
Fragments arranged by size:
3489 1813 453
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4250 4424 65
Size: 1183 3002 174 1396
Fragments arranged by size:
3002 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2114 2888 4399 4586 1700
Size: 110 304 774 1511 187 2869
Fragments arranged by size:
2869 1511 774 304 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 2416 1925
Size: 491 5264
BssSI C'ACGA_G
Cuts at: 2723 3815 4827 2723
Size: 1092 1012 3651
Fragments arranged by size:
3651 1092 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2512 794
Size: 1718 4037
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 2479 1184
Size: 1295 4460
BstXI CCAn_nnnn'nTGG
Cuts at: 2047 2047
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2569 2625 2946 3085 3452 3837 3854 5306
Size: 597 56 321 139 367 385 17 1452
Cuts at: 5306 5392 5404 5750 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 597 385 367 346 321 139
86 56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4264
Fragments arranged by size:
4264 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2647 487
Size: 683 56 1421 3595
Fragments arranged by size:
3595 1421 683 56
BtsI GCAGTG_nn'
Cuts at: 2629 3271 4065 4085 2629
Size: 642 794 20 4299
Fragments arranged by size:
4299 794 642 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3025 3060 529
Size: 35 2461 35 3224
Fragments arranged by size:
3224 2461 35 35
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 3202 4091
Size: 54 1425 50 275 18 408 964 889
Cuts at: 4091 5720 8
Size: 1629 43
Fragments arranged by size:
1629 1425 964 889 408 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2123 2178 2795 3056 3685 1353
Size: 770 55 617 261 629 3423
Fragments arranged by size:
3423 770 629 617 261 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2920 2920
Size: 5755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 2489 3818 4415 5202 5470 1239
Size: 393 391 466 1329 597 787 268 1524
Fragments arranged by size:
1524 1329 787 597 466 393 391 268
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 5755
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 2476 2608 2672 2679 4902 1678
Size: 798 132 64 7 2223 2531
Fragments arranged by size:
2531 2223 798 132 64 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2159 2191 4396 4428 4470
Size: 32 135 32 587 32 2205 32 42
Cuts at: 4470 4502 1373
Size: 32 2626
Fragments arranged by size:
2626 2205 587 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2343 2487 4869 5053 1305
Size: 1038 144 2382 184 2007
Fragments arranged by size:
2382 2007 1038 184 144
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2240 10
Size: 54 1425 343 408 3525
Fragments arranged by size:
3525 1425 408 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2903 3713 4072 4231 518
Size: 648 1737 810 359 159 2042
Fragments arranged by size:
2042 1737 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2377 3171 3848 4996 5241 1606
Size: 771 794 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 771 677 245
NaeI GCC'GGC
Cuts at: 2890 2890
Size: 5755
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4264
Fragments arranged by size:
4264 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NgoMIV G'CCGG_C
Cuts at: 2888 2888
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NruI TCG'CGA
Cuts at: 2524 2524
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2722 2929 3113 4658 1132
Size: 97 1493 207 184 1545 2229
Fragments arranged by size:
2229 1545 1493 207 184 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5755
PfoI T'CCnGG_A
Cuts at: 2123 2853 2123
Size: 730 5025
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2540 2572 3824 3856 5337 5369 2540
Size: 32 1252 32 1481 32 2926
Fragments arranged by size:
2926 1481 1252 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PspXI vC'TCGA_Gb
Cuts at: 2192 2915 2192
Size: 723 5032
PstI C_TGCA'G
Cuts at: 830 3107 830
Size: 2277 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
SacI G_AGCT'C
Cuts at: 2922 2922
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SapI GCTCTTCn'nnn_
Cuts at: 2123 2795 2123
Size: 672 5083
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 3103 4238 4919 5110 826
Size: 219 2058 1135 681 191 1471
Fragments arranged by size:
2058 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 2487 3082 1872
Size: 615 595 4545
Fragments arranged by size:
4545 615 595
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 2915 3859 4760 5022 820
Size: 197 926 249 723 944 901 262 1553
Fragments arranged by size:
1553 944 926 901 723 262 249 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 2722 3113 1132
Size: 1590 391 3774
Fragments arranged by size:
3774 1590 391
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 2405 1517
Size: 888 4867
StyI C'CwwG_G
Cuts at: 513 1065 2004 2352 3194 513
Size: 552 939 348 842 3074
Fragments arranged by size:
3074 939 842 552 348
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2099 3782 3799 3952 4137 4582 325
Size: 1255 519 1683 17 153 185 445 1498
Fragments arranged by size:
1683 1498 1255 519 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4001 5713 96
Size: 274 80 33 51 1443 2024 1712 138
Fragments arranged by size:
2024 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2253 2285 5715 5747 2253
Size: 32 3430 32 2261
Fragments arranged by size:
3430 2261 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3005 1316
Size: 1689 4066
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 2192 2915 1943
Size: 249 723 4783
Fragments arranged by size:
4783 723 249
XmaI C'CCGG_G
Cuts at: 1870 2485 3080 1870
Size: 615 595 4545
Fragments arranged by size:
4545 615 595
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2778 2809 3563 276
Size: 53 83 186 936 1244 31 754 2468
Fragments arranged by size:
2468 1244 936 754 186 83 53 31
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BfrBI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI
NarI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI