Restriction map for
pFN21AE3134
(Circular) (Six-base) MAPSORT of: pFN21AE3134.seq Check: 4780 from: 1 to: 5725
pFN21AE3134 5725 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2787 3068 1937
Size: 850 281 4594
Fragments arranged by size:
4594 850 281
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2292 3788 5172 5440 1632
Size: 660 1496 1384 268 1917
Fragments arranged by size:
1917 1496 1384 660 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2911 1184
Size: 1727 3998
AlwNI CAG_nnn'CTG
Cuts at: 3468 5040 3468
Size: 1572 4153
ApaI G_GGCC'C
Cuts at: 2978 2978
Size: 5725
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 2716 2838 3034 3260 3412 5521 1493
Size: 1223 122 196 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 196 152 122
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 3050 1870
Size: 73 1107 4545
Fragments arranged by size:
4545 1107 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3046 4501 618
Size: 325 217 627 1259 1455 1842
Fragments arranged by size:
1842 1455 1259 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2943 2978 3044 1391
Size: 1552 35 66 4072
Fragments arranged by size:
4072 1552 66 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3916 3950 1903
Size: 34 50 34 1895 34 3678
Fragments arranged by size:
3678 1895 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2772 3617 4827 2772
Size: 845 1210 3670
Fragments arranged by size:
3670 1210 845
BclI T'GATC_A
Cuts at: 1548 2829 1548
Size: 1281 4444
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4336 136
Size: 107 122 71 631 605 2664 1525
Fragments arranged by size:
2664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5720 5720
Size: 5725
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 2978 3786 4942 1790
Size: 1188 808 1156 2573
Fragments arranged by size:
2573 1188 1156 808
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2366 3456 4414
Size: 416 53 83 399 24 949 1090 958
Cuts at: 4414 442
Size: 1753
Fragments arranged by size:
1753 1090 958 949 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2451 2793 4385 1239
Size: 1212 342 1592 2579
Fragments arranged by size:
2579 1592 1212 342
Bpu10I CC'TnA_GC
Cuts at: 2282 2516 2282
Size: 234 5491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3850 4715 5013 3850
Size: 865 298 4562
Fragments arranged by size:
4562 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4388 882
Size: 617 2889 2219
Fragments arranged by size:
2889 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3532 3914 4549 275
Size: 53 83 186 936 1999 382 635 1451
Fragments arranged by size:
1999 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4158 4556 4830 4977 5388 4158
Size: 398 274 147 411 4495
Fragments arranged by size:
4495 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2902 2932 1178
Size: 30 165 30 1499 30 3971
Fragments arranged by size:
3971 1499 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5725
BseYI C'CCAG_C
Cuts at: 2013 2604 4928 5424 2013
Size: 591 2324 496 2314
Fragments arranged by size:
2324 2314 591 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3175 3936 4964 1540
Size: 275 186 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3014 3786 3871 4942 3014
Size: 772 85 1071 3797
Fragments arranged by size:
3797 1071 772 85
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2943 2978 3014 3044 3786 3871 4942
Size: 399 1153 35 36 30 742 85 1071
Cuts at: 4942 1391
Size: 2174
Fragments arranged by size:
2174 1153 1071 742 399 85 36 35
30
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3080 844
Size: 2236 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 1394
Size: 429 1789 3507
Fragments arranged by size:
3507 1789 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4220 4394 65
Size: 1183 2972 174 1396
Fragments arranged by size:
2972 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4369 4556 1700
Size: 110 2559 187 2869
Fragments arranged by size:
2869 2559 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 3785 4797 3785
Size: 1012 4713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstXI CCAn_nnnn'nTGG
Cuts at: 2151 2936 2151
Size: 785 4940
BstYI r'GATC_y
Cuts at: 1972 2243 2413 3055 3422 3807 3824 5276 5362
Size: 271 170 642 367 385 17 1452 86
Cuts at: 5362 5374 5720 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 642 385 367 346 271 170
86 17 12
BstZ17I GTA'TAC
Cuts at: 2788 2788
Size: 5725
BtgI C'CryG_G
Cuts at: 513 1065 2851 513
Size: 552 1786 3387
Fragments arranged by size:
3387 1786 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 2462 3241 4035 4055 2462
Size: 779 794 20 4132
Fragments arranged by size:
4132 794 779 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2532 2567 529
Size: 35 1968 35 3687
Fragments arranged by size:
3687 1968 35 35
DraI TTT'AAA
Cuts at: 2844 3029 2844
Size: 185 5540
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2926 3172 4061
Size: 54 1425 50 275 18 1096 246 889
Cuts at: 4061 5690 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1096 889 275 246 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2370 2730 3655 1353
Size: 1017 360 925 3423
Fragments arranged by size:
3423 1017 925 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2292 2451 2793 3788 4385 5172 5440
Size: 393 660 159 342 995 597 787 268
Cuts at: 5440 1239
Size: 1524
Fragments arranged by size:
1524 995 787 660 597 393 342 268
159
EcoO109I rG'GnC_Cy
Cuts at: 2974 2974
Size: 5725
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 4872 1678
Size: 3194 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373
Size: 32 135 32 2794 32 42 32 2626
Fragments arranged by size:
2794 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2039 748
Size: 1291 4434
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2049 2508 4839 5023 1305
Size: 744 459 2331 184 2007
Fragments arranged by size:
2331 2007 744 459 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2928 10
Size: 54 1425 343 1096 2807
Fragments arranged by size:
2807 1425 1096 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2484 2867 3683 4042 4201 518
Size: 648 1318 383 816 359 159 2042
Fragments arranged by size:
2042 1318 816 648 383 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 3141 3818 4966 5211 1606
Size: 411 1124 677 1148 245 2120
Fragments arranged by size:
2120 1148 1124 677 411 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5173
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 2799 3083 4628 1132
Size: 97 1570 284 1545 2229
Fragments arranged by size:
2229 1570 1545 284 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5725
PfoI T'CCnGG_A
Cuts at: 2428 2770 2428
Size: 342 5383
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PspOMI G'GGCC_C
Cuts at: 2974 2974
Size: 5725
PstI C_TGCA'G
Cuts at: 830 2457 3077 830
Size: 1627 620 3478
Fragments arranged by size:
3478 1627 620
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 2017 2017
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 2453 2695 2729 3073 4208 4889 5080
Size: 219 1408 242 34 344 1135 681 191
Cuts at: 5080 826
Size: 1471
Fragments arranged by size:
1471 1408 1135 681 344 242 219 191
34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3829 4730 4992 820
Size: 197 926 1886 901 262 1553
Fragments arranged by size:
1886 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 2342 3649 5
Size: 47 2290 1307 2081
Fragments arranged by size:
2290 2081 1307 47
StuI AGG'CCT
Cuts at: 1517 2762 1517
Size: 1245 4480
StyI C'CwwG_G
Cuts at: 513 1065 2808 3164 513
Size: 552 1743 356 3074
Fragments arranged by size:
3074 1743 552 356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3752 3769 3922 4107 4552 325
Size: 1255 2172 17 153 185 445 1498
Fragments arranged by size:
2172 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3971 5683 96
Size: 274 80 33 51 1443 1994 1712 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2627 2659 2902 2934 5685 5717 2627
Size: 32 243 32 2751 32 2635
Fragments arranged by size:
2751 2635 243 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 2305 3854 2305
Size: 1549 4176
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI