Restriction map for
pFN21AE3133
(Circular) (Six-base) MAPSORT of: pFN21AE3133.seq Check: 5083 from: 1 to: 5722
pFN21AE3133 5722 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3532 278
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3065 1937
Size: 1128 4594
Acc65I G'GTAC_C
Cuts at: 3043 3043
Size: 5722
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2606 3785 5169 5437 1632
Size: 974 1179 1384 268 1917
Fragments arranged by size:
1917 1384 1179 974 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 2275 3019 4621 5598 1203
Size: 1072 744 1602 977 1327
Fragments arranged by size:
1602 1327 1072 977 744
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 2881 4451 2881
Size: 1570 4152
AleI CACnn'nnGTG
Cuts at: 1166 2526 1166
Size: 1360 4362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5722
AlwNI CAG_nnn'CTG
Cuts at: 3465 5037 3465
Size: 1572 4150
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 2710 3031 3257 3409 5518 1493
Size: 1217 321 226 152 2109 1697
Fragments arranged by size:
2109 1697 1217 321 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4276 160
Size: 872 3244 1606
Fragments arranged by size:
3244 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 3047 1870
Size: 73 1104 4545
Fragments arranged by size:
4545 1104 73
BamHI G'GATC_C
Cuts at: 3052 3419 3052
Size: 367 5355
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3043 4498 618
Size: 325 217 627 1256 1455 1842
Fragments arranged by size:
1842 1455 1256 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3041 1391
Size: 1650 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2553 928
Size: 971 654 4097
Fragments arranged by size:
4097 971 654
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 1903
Size: 34 1976 34 3678
Fragments arranged by size:
3678 1976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3614 4824 3614
Size: 1210 4512
BclI T'GATC_A
Cuts at: 1548 2332 1548
Size: 784 4938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2445 4333 136
Size: 107 122 71 631 605 773 1888 1525
Fragments arranged by size:
1888 1525 773 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5717 5717
Size: 5722
BlpI GC'TnA_GC
Cuts at: 3139 3139
Size: 5722
Bme1580I G_kGCm'C
Cuts at: 1790 2410 3783 4939 1790
Size: 620 1373 1156 2573
Fragments arranged by size:
2573 1373 1156 620
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3453 4411 442
Size: 416 53 83 399 24 2036 958 1753
Fragments arranged by size:
2036 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4382 1239
Size: 3143 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3847 4712 5010 3847
Size: 865 298 4559
Fragments arranged by size:
4559 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4385 882
Size: 617 2886 2219
Fragments arranged by size:
2886 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3513 493
Size: 711 2309 2702
Fragments arranged by size:
2702 2309 711
BsaBI GATnn'nnATC
Cuts at: 5716 5716
Size: 5722
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3529 3911 4546 275
Size: 53 83 186 936 1996 382 635 1451
Fragments arranged by size:
1996 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4155 4553 4827 4974 5385 4155
Size: 398 274 147 411 4492
Fragments arranged by size:
4492 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2166 2196 2613 2643 1178
Size: 30 165 30 763 30 417 30 4257
Fragments arranged by size:
4257 763 417 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2942 1186
Size: 910 846 3966
Fragments arranged by size:
3966 910 846
BseYI C'CCAG_C
Cuts at: 4925 5421 4925
Size: 496 5226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 1163
Size: 573 705 4444
Fragments arranged by size:
4444 705 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3172 3933 4961 1540
Size: 275 186 1171 761 1028 2301
Fragments arranged by size:
2301 1171 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2523 3783 3868 4939 2523
Size: 1260 85 1071 3306
Fragments arranged by size:
3306 1260 1071 85
BsmI GAATG_Cn'
Cuts at: 3233 3326 3233
Size: 93 5629
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2410 2523 3041 3783 3868 4939 1391
Size: 399 620 113 518 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 620 518 399 113 85
BspEI T'CCGG_A
Cuts at: 5385 5385
Size: 5722
BspHI T'CATG_A
Cuts at: 2872 3611 5341 2872
Size: 739 1730 3253
Fragments arranged by size:
3253 1730 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3077 844
Size: 2233 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3609 1394
Size: 429 1786 3507
Fragments arranged by size:
3507 1786 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4217 4391 65
Size: 1183 2969 174 1396
Fragments arranged by size:
2969 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4366 4553 1700
Size: 110 2556 187 2869
Fragments arranged by size:
2869 2556 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5722
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 3782 4794 3782
Size: 1012 4710
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2419 794
Size: 1625 4097
BstBI TT'CG_AA
Cuts at: 2281 4526 2281
Size: 2245 3477
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 3052 3419 3804 3821 5273 5359 5371 5717
Size: 1080 367 385 17 1452 86 12 346
Cuts at: 5717 1972
Size: 1977
Fragments arranged by size:
1977 1452 1080 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2440 513
Size: 552 1375 3795
Fragments arranged by size:
3795 1375 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4983
Fragments arranged by size:
4983 683 56
BtsI GCAGTG_nn'
Cuts at: 2408 3238 4032 4052 2408
Size: 830 794 20 4078
Fragments arranged by size:
4078 830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5687
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4729 809
Size: 507 3413 1802
Fragments arranged by size:
3413 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3169 4058 5687
Size: 54 1425 50 275 18 1339 889 1629
Cuts at: 5687 8
Size: 43
Fragments arranged by size:
1629 1425 1339 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3169 1537
Size: 275 1357 4090
Fragments arranged by size:
4090 1357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2163 2278 3652 1353
Size: 810 115 1374 3423
Fragments arranged by size:
3423 1374 810 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4309 4683 4829 387
Size: 1064 138 2720 374 146 1280
Fragments arranged by size:
2720 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2606 3785 4382 5169 5437 1239
Size: 393 974 1179 597 787 268 1524
Fragments arranged by size:
1524 1179 974 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3031 3409 3031
Size: 378 5344
EcoRV GAT'ATC
Cuts at: 2503 2503
Size: 5722
FspI TGC'GCA
Cuts at: 4228 4228
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 4869 1678
Size: 3191 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2713 2745 2867 2899 4363
Size: 32 135 32 1141 32 122 32 1464
Cuts at: 4363 4395 4437 4469 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1464 1141 135 122 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2050 2491 3066 3312 669
Size: 988 281 112 441 575 246 3079
Fragments arranged by size:
3079 988 575 441 281 246 112
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5722
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KpnI G_GTAC'C
Cuts at: 3047 3047
Size: 5722
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4836 5020 1305
Size: 3531 184 2007
Fragments arranged by size:
3531 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3900
Fragments arranged by size:
3900 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2526 3680 4039 4198 518
Size: 648 1360 1154 359 159 2042
Fragments arranged by size:
2042 1360 1154 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2452 3138 3815 4963 5208 1606
Size: 846 686 677 1148 245 2120
Fragments arranged by size:
2120 1148 846 686 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5170
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5722
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 3080 4625 1132
Size: 97 1851 1545 2229
Fragments arranged by size:
2229 1851 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5722
PciI A'CATG_T
Cuts at: 4621 4621
Size: 5722
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5722
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2277 2309 3791 3823 5304 5336 2277
Size: 32 1482 32 1481 32 2663
Fragments arranged by size:
2663 1482 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PstI C_TGCA'G
Cuts at: 830 3074 830
Size: 2244 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
PvuII CAG'CTG
Cuts at: 2452 2452
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SapI GCTCTTCn'nnn_
Cuts at: 2163 2163
Size: 5722
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SfcI C'TryA_G
Cuts at: 826 1045 2323 2524 3000 3070 4205 4886 5077
Size: 219 1278 201 476 70 1135 681 191
Cuts at: 5077 826
Size: 1471
Fragments arranged by size:
1471 1278 1135 681 476 219 201 191
70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5722
SmaI CCC'GGG
Cuts at: 1872 3049 1872
Size: 1177 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3826 4727 4989 820
Size: 197 926 1883 901 262 1553
Fragments arranged by size:
1883 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 3080 1132
Size: 1948 3774
SspI AAT'ATT
Cuts at: 5 52 3646 5
Size: 47 3594 2081
Fragments arranged by size:
3594 2081 47
StuI AGG'CCT
Cuts at: 1517 2609 1517
Size: 1092 4630
StyI C'CwwG_G
Cuts at: 513 1065 3161 513
Size: 552 2096 3074
Fragments arranged by size:
3074 2096 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3749 3766 3919 4104 4549 325
Size: 1255 2169 17 153 185 445 1498
Fragments arranged by size:
2169 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3968 5680 96
Size: 274 80 33 51 1443 1991 1712 138
Fragments arranged by size:
1991 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2700 2732 5682 5714 2700
Size: 32 2950 32 2708
Fragments arranged by size:
2950 2708 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2235 1316
Size: 919 4803
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5722
XmaI C'CCGG_G
Cuts at: 1870 3047 1870
Size: 1177 4545
XmnI GAAnn'nnTTC
Cuts at: 2766 3851 2766
Size: 1085 4637
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3530 276
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI